Is shrimp farming a successful adaptation to salinity intrusion? A geospatial associative analysis of poverty in the populous Ganges–Brahmaputra–Meghna Delta of Bangladesh

The Ganges–Brahmaputra–Meghna delta of Bangladesh is one of the most populous deltas in the world, supporting as many as 140 million people. The delta is threatened by diverse environmental stressors including salinity intrusion, with adverse consequences for livelihood and health. Shrimp farming is recognised as one of the few economic adaptations to the impacts of the rapidly salinizing delta. Although salinity intrusion and shrimp farming are geographically co-located in the delta, there has been no systematic study to examine their geospatial associations with poverty. In this study, we use multiple data sources including Census, Landsat Satellite Imagery and soil salinity survey data to examine the extent of geospatial clustering of poverty within the delta and their associative relationships with salinity intensity and shrimp farming. The analysis was conducted at the union level, which is the lowest local government administrative unit in Bangladesh. The findings show a strong clustering of poverty in the delta, and whilst different intensities of salinization are significantly associated with increasing poverty, neither saline nor freshwater shrimp farming has a significant association with poverty. These findings suggest that whilst shrimp farming may produce economic growth, in its present form it has not been an effective adaptation for the poor and marginalised areas of the delta. The study demonstrates that there are a series of drivers of poverty in the delta, including salinization, water logging, wetland/mudflats, employment, education and access to roads, amongst others that are discernible spatially, indicating that poverty alleviation programmes in the delta require strengthening with area-specific targeted interventions.     

Identification of lactic acid bacteria isolated from wine using real-time PCR

Different lactic acid bacteria strains have been shown to cause wine spoilage, including the generation of substances undesirable for the health of wine consumers. The aim of this study was to investigate the occurrence of selected species of heterofermentative lactobacilli, specifically Lactobacillus brevis, Lactobacillus hilgardii, and Lactobacillus plantarum in six different Slovak red wines following the fermentation process. In order to identify the dominant Lactobacillus strain using quantitative (real time) polymerized chain reaction (qPCR) method, pure lyophilized bacterial cultures from the Czech Collection of Microorganisms were used. Six different red wine samples following malolactic fermentation were obtained from selected wineries. After collection, the samples were subjected to a classic plate dilution method for enumeration of lactobacilli cells. Real-time PCR was performed after DNA extraction from pure bacterial strains and wine samples. We used SYBR® Green master mix reagents for measuring the fluorescence in qPCR. The number of lactobacilli ranged from 3.60 to 5.02 log CFU mL^?1. Specific lactobacilli strains were confirmed by qPCR in all wine samples. The number of lactobacilli ranged from 10³ to 10⁶ CFU mL^?1. A melting curve with different melting temperatures (T_m) of DNA amplicons was obtained after PCR for the comparison of T_m of control and experimental portions, revealing that the most common species in wine samples was Lactobacillus plantarum with a T_m of 84.64°C.     

On a Failed Defense of Factory Farming

Timothy Hsiao attempts to defend industrial animal farming by arguing that it is not inherently cruel. We raise three main objections to his defense. First, his argument rests on a misunderstanding of the nature of cruelty. Second, his conclusion, though technically true, is so weak as to be of virtually no moral significance or interest. Third, his contention that animals lack moral standing, and thus that mistreating them is wrong only insofar as it makes one more disposed to mistreat other humans, is untenable on both philosophical and biological grounds.     

Comparative study of accessibility of distinctive pesticides

Interactions of micro-contaminants with soil may play a crucial role in their environmental fate and possible harmful effects. Major goals of our investigations were to model the availability of widely used pesticides and characterize adsorption capabilities of distinctive soil types by the accomplishment of extensive comparative studies and application of several extraction methods. Environmental and biological relevance of these examinations is enhanced by the fact that intrinsic features and specific details of pesticide accessibility have not been revealed so far by a comparative approach. Five different experimental methods were assessed for modelling accessibility of five selected pesticides. The applied models for regaining the pesticides showed diverse efficiency in extraction capability in cases of the different soil types (sandy, brown forest and alluvial soils). The amounts of the obtained pesticides were determined by using gas-chromatography coupled to mass spectrometry (GC-MS) and high pressure liquid chromatography coupled to mass spectrometry (HPLC-MS). Accessibility of pesticides was also compared in cases of sterilized and real soil samples in order to estimate the extent of the influence of microflora. Aqueous extraction solvents proved to be suitable for accurate assessment of the accessible amounts of pesticides, as their effectivity was at least as high as that of the applied organic solvents. In our studies pesticide-soil interactions have comprehensively been characterized, and possible influences of environmental factors on the accessibility have also been revealed. Our study might be regarded as a tentative approach to model some significant circumstances playing key roles in pesticides' possible bioavailability.     

Nanobioaerosols--reconsidering agricultural irrigation in a warming world

Nanobacteria are best described as 60-300 nm nanovesicles. In the body they collect calcium and phosphate to form apatite, adhere to cells, or invade them--processes regulated by a slime based on proteins (primordial proteins). A versatile functionality realized with a minimum of properties equips nanobacteria with a unique survival potential. They were identified in humans, animals, wastewater and the stratosphere. In South Africa they were detected in people infected with HIV. Models indicate that they boost the genetic diversity of the HIV-1 virus. Experiments showed that they are excreted via urine, explaining their presence in the environment. Eradication would be virtually impossible if they had an extraterrestrial origin, implying a permanent bombardment from space. Whereas the biological status of nanobacteria is still not clarified, we postulate here that the native habitat of nanobacteria are mammals, suggesting that at least modern species have their origin on Earth. The thesis results from mapping functions and properties of the slime, and could facilitate the localisation of nanobacterial reservoirs, identification of local distribution routes and tracking of global transport cycles. Agricultural irrigation with water containing excreta from humans infected with nanobacteria could be a central disseminator of the nanobioaerosols.     

Effects of leaching from alkaline red mud on soil biota: modelling the conditions after the Hungarian red mud disaster

A soil column experiment was set up to investigate the effect of red mud from Ajka (Hungary) on a typical soil profile from the affected area. The chemical changes caused by the red mud leachate and the effects of these changes on living organisms were assessed. Ecotoxicological tests were performed with Vibrio fischeri, Sinapis alba and Folsomia candida and the number of aerobic heterotrophic microorganisms was determined. The total, plant-available, exchangeable and water-soluble fractions of Na, Mo, Cu and Cr increased in the soil, mostly owing to their leaching from the red mud layer, but partly to the increase in the pH and DOC concentration. The chemical changes only had significant effects on the test organisms in the 0–30 cm soil layer, except for F. candida, which also had a lower survival rate in the 30–50 cm soil layer. No severe toxic effects were detected in the test organisms; in fact a stimulating effect was revealed for the aerobic heterotrophic cell number and for S. alba germination. However, the red mud itself was toxic, so the ecotoxicological tests justified the removal of red mud from the soil surface after the disaster.     

Acetone-induced photodechlorination of Aroclor 1254 in alkaline 2-propanol: Probing the mechanism by thermolysis in the presence of di-t- butyl peroxide

Aroclor 1254 (1860 mg/l) in alkaline 2-propanol and in the presence of acetone (4% v/v) was photodechlorinated to biphenyl at λ >300 nm with exceptionally high quantum yield (Φ= 18). Under similar conditions photodechlorination of extracts of Aroclor 1254 contaminated soil (730 mg/kg) proceeded with lower quantum yield Φ= 0.4. While oxygen severely quenched photolysis, dechlorination was accomplished under thermal conditions using di-t-butyl peroxide, t-BuOOBu-t, as free radical initiator. A free radical chain reaction is suggested in which acetone triplet, T₁(n,π*), or t-BuO^· radical abstracts H-atom from 2-propanol to give the ketyl radical, (CH₃)₂ OH, which after losing a proton to the alkaline medium gives the ketyl radical anion, (CH₃)₂CHO^·−. The Aroclor in turn reacts with the latter species through an electron-transfer process giving unstable aryl radical anion, , which cleaves releasing the chloride anion, Cl⁻ and the aryl radical, A^·     

Sperm traits of the quacking frog, Crinia georgiana: intra- and interpopulation variation in a species with a high risk of sperm competition

Sperm traits often show extreme variation both between and within species. Between-species variation may often be interpreted in the context of a sperm competition theory, but within-species variation has remained unexplained. Previous studies on intraspecific variation in sperm traits have focused on a limited set of variables and may have failed to explain observed variation because of potential trade-offs between different sperm traits. We report on variation in number, size, motility and longevity of sperm in the frog Crinia georgiana, a species where sperm competition is common. We found intrapopulation variation in sperm size and motility and interpopulation variation in relative sperm number and size. When we combined relative sperm number and size into one variable, and motility and longevity into another, we found significant interpopulation variation in these variables as well. We also detected considerable intra- and significant interpopulation variation in cumulative sperm quality, a combination of all four sperm traits. Furthermore, a significant effect of the interaction between population origin and male size on sperm characteristics indicated interpopulation variation in the strength of selection acting on sperm traits of males adopting different mating strategies. We discuss heterogeneity in the reproductive environment, a complex genetic background in the determination of sperm characteristics and varying levels of developmental noise as potential contributors to the observed variation in sperm traits.     

Evaluation of a diode laser based photoacoustic instrument combined with preconcentration sampling for measuring surface–atmosphere exchange of ammonia with the aerodynamic gradient method

We present here a novel instrument for measuring surface–atmosphere exchange fluxes of ammonia. The instrument is the upgraded version of a recently developed near-infrared diode laser based photoacoustic ammonia concentration monitoring instrument, i.e. the original instrument is supplemented with two additional sampling lines, an appropriate gas handling system and an advanced software controlling gradient measurements. As a result of these developments, ammonia concentration can be measured simultaneously at three different heights above the ground and ammonia fluxes can be calculated from these data using the aerodynamic gradient method. The instrument operates fully automatically, requires minimal maintenance and has a temperature controlled, waterproof housing which makes it suitable for measurements even under harsh field conditions. Preliminary tests on stability and accuracy were carried out during two two-week field measurement campaigns, with the three sampling inlets being placed at the same height together with the inlet of a reference instrument. The readings of the three channels agreed well (with correlation coefficients above 0.96). Comparison to reference instruments showed good stability of the photoacoustic instrument, there was no measurable zero-drift or change in sensitivity during the tests. Flux measurements were carried out during a three-week field campaign in southern Scotland over fertilized grassland with reference to a wet-chemical AMANDA instrument in gradient configuration. Ammonia fluxes calculated from the data of the two instruments agreed well. Fluxes up to 2500 ng m^?2 s^?1 were observed after fertilization. The minimum detectable ammonia flux was calculated on the basis of “virtual ammonia fluxes”, from measurements carried out with all inlets at the same height and was found to be ±60 ng m^?2 s^?1 which ensures reliable measurements above intensively managed grasslands or agricultural fields.