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11.
Marize Pereira Miagostovich Flávia Ramos Guimarães Carmen Baur Vieira Tulio Machado Fumian Nilson Porto da Gama Matias Victoria Jaqueline Mendes de Oliveira Anna Carolina de Oliveira Mendes Ana Maria Coimbra Gaspar José Paulo Gagliardi Leite 《Food and environmental virology》2014,6(2):110-115
The preservation of water resources is one of the goals of the designation of parks that act as natural reservoirs. In order to assess the impact of the presence of humans in an environmental preservation area bordering urban areas, the presence of four pathogenic enteric viruses [group A rotavirus (RV-A), norovirus (NoV), human adenoviruses (HAdV), and hepatitis A virus (HAV)], as well as the physico-chemical parameters, and Escherichia coli levels were assessed in riverine water samples. From June 2008 to May 2009, monthly monitoring was performed along the Engenho Novo River. RV-A, NoV, and HAdV were observed in 29 % (31/108) of the water samples, with concentrations of up to 103 genome copies/liter. The natural occurrence of infectious HAdV was demonstrated by Integrated Cell Culture-PCR (ICC-PCR). This study confirms the suitability of using the detection of fecal-oral transmitted viruses as a marker of human fecal contamination in water matrices and indicates the spread of pathogenic viruses occurring in an alleged area of environmental protection. 相似文献
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Urban wastewater treatment plant efficiency, as well as biosolid quality, depends on urban wastewater quality, which can be affected by non domestic discharges (industrial, commercial etc.). The characterization of wastewater quality and non domestic discharge is complex, expensive and time consuming. However, these discharges must be controlled and reduced if possible. The development of a simple and fast methodology, namely based on alternative methods such as UV spectrophotometry, has been carried out and applied to different areas of a medium sized town of Southern Québec (Canada). Several autosamplers and on line/on site measurements have been used in critical control points of the network areas, for a dry weather campaign in four areas (industrial, commercial, hospital and university). The flow rate study, completed by the exploitation of conductivity measurements and the qualitative examination of UV spectra allows the discrimination of non domestic loads and their variability study from one point to another. The identification of critical discharges and organic shock loads has been possible with low investment, and mitigation actions have been proposed. 相似文献
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Larvae of the moth Cydalima perspectalis are specialized on box trees (Buxus spp.). Native to eastern Asia, the moth has been introduced to Europe in 2007 and is nowadays causing severe damage to box trees in private and public gardens, as well as in semi-natural box tree forests. Box trees contain highly toxic triterpenoid alkaloids which may be sequestered by specialized herbivores such as C. perspectalis. We determined the alkaloid composition in leaves of the five most common box tree varieties in Europe belonging to two Buxus species using liquid chromatography–mass spectrometry (LC–MS) metabolite profiling. We also examined whether larvae and moths of C. perspectalis accumulate alkaloids from the different box tree varieties. The differences in alkaloid composition observed between the box tree species Buxus sempervirens and Buxus microphylla were mirrored in the tissue of C. perspectalis larvae fed on either of the different box tree species, indicating uptake of alkaloids. The larvae stored large amounts of dibasic alkaloids in their body, while monobasic alkaloids were metabolized and/or excreted. Newly emerged adult moths contained no traces of alkaloids. 相似文献
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The influence of management intensity on the richness, abundance and composition of land snail species was examined in 21 calcareous, nutrient-poor cattle pastures in the northwestern Jura mountains, Switzerland. Grazing intensity was positively correlated with the extent of fertilization of the pastures. Pastures without fertilizer application and with low grazing intensity harboured more snail species and more threatened snails than pastures with annual addition of manure or pastures with manure and nitrogen fertilizer and higher grazing intensity. Fewer snail individuals, open-land species and open-land individuals were found on pastures with high than on pastures with low management intensity. To preserve the threatened snail species in dry, nutrient-poor grasslands, a network of pastures should be managed without fertilization and grazing intensity should not exceed 180 LU ha−1 d (product of livestock units per hectare and grazing days). 相似文献
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The Science of Nature - 相似文献
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Exposed limestone cliffs in central Europe harbor a highly divers flora with many rare and endangered species. During the
past few decades, there has been increasing recreational use of these cliffs, which has caused local environmental disturbances.
Successful restoration strategies hinge on identifying critical limitations. We examined the composition of aboveground forest
vegetation and density and species composition of seeds in the soil seed bank at the base of four limestone cliffs in mixed
deciduous forests that are intensively disturbed by human trampling and at four undisturbed cliffs in the Jura Mountains in
northwestern Switzerland. We found that long-term human trampling reduced total aboveground vegetation cover at the base of
cliffs and caused a significant shift in the plant-species composition. Compared with undisturbed cliffs, total seed density
was lower in disturbed cliffs. Human trampling also altered the species composition of seeds in the soil seed bank. Seeds
of unintentionally introduced, stress-tolerant, and ruderal species dominated the soil seed bank at the base of disturbed
cliffs. Our findings indicate that a restoration of degraded cliff bases from the existing soil seed bank would result in
a substantial change of the original unique plant composition. Active seed transfer, or seed flux from adjacent undisturbed
forest areas, is essential for restoration success. 相似文献
20.
Carmen Baur Vieira Adriana de Abreu Corrêa Michele Silva de Jesus Sérgio Luiz Bessa Luz Peter Wyn-Jones David Kay Mônica Simões Rocha Marize Pereira Miagostovich 《Food and environmental virology》2017,9(2):195-207
During the Amazonian flood season in 2012, the Negro River reached its highest level in 110 years, submerging residential and commercial areas which appeared associated with an elevation in the observed gastroenteritis cases in the city of Manaus. The aim of this study was to evaluate the microbiological water quality of the Negro River basin during this extreme flood to investigate this apparent association between the illness cases and the population exposed to the contaminated waters. Forty water samples were collected and analysed for classic and emerging enteric viruses. Human adenoviruses, group A rotaviruses and genogroup II noroviruses were detected in 100, 77.5 and 27.5% of the samples, respectively, in concentrations of 103–106 GC/L. All samples were compliant with local bacteriological standards. HAdV2 and 41 and RVA G2, P[6], and P[8] were characterised. Astroviruses, sapoviruses, genogroup IV noroviruses, klasseviruses, bocaviruses and aichiviruses were not detected. Statistical analyses showed correlations between river stage level and reported gastroenteritis cases and, also, significant differences between virus concentrations during this extreme event when compared with normal dry seasons and previous flood seasons of the Negro River. These findings suggest an association between the extreme flood experienced and gastrointestinal cases in the affected areas providing circumstantial evidence of causality between the elevations in enteric viruses in surface waters and reported illness. 相似文献