Specificity and sensitivity of microsatellite markers for the identification of larvae

The identification of larval marine invertebrates to species or even higher taxonomic levels by morphological examination is notoriously difficult. Many diagnostic features are absent or poorly formed at early stages in development. This is particularly true for the larvae of bivalve molluscs, for which a routine and accurate method of identification would prove valuable to both ecologists and fishery managers. A simple molecular genetic method to identify specifically larvae of the European oyster, Ostrea edulis L., 1758, is presented. The test is based on PCR amplification of highly species-specific microsatellite loci and is sensitive enough to register the presence of a single larval individual (~200 µm width) in a mixed sample of 20 mg wet weight plankton (approximately 250 larval animals). This work demonstrates that microsatellite loci can be used as highly sensitive and specific taxonomic indicators, for studies of planktonic larvae. Details of three novel microsatellite loci are also given for O. edulis, increasing the suite of molecular tools available for use in population genetic studies of this commercially important species.     

A Successful and Productive Year

It is gratifying to report that 1975-1976 has been another year of increased activity for the Air Pollution Control Association, and various indicators are positive compared to previous years. Membership has increased, attendance at meetings has increased, we have a substantial surplus to report, there is increased activity in membership interest and in our overall activity. Public affairs involvement has increased somewhat, as have our international activities. Local sections and technical committees have expanded their scope of productive involvement. Clearly, APCA has had a successful and productive year.     

Displacement as a predictor of functional impairment in tsunami‐exposed children

Thirty months after the Indian Ocean tsunami of 26 December 2004, thousands of families in Aceh Province, Indonesia, remained in temporary barracks while sanitation conditions and non‐governmental organisation support deteriorated. This study sought to determine the factors associated with functional impairment in a sample of 138 displaced and non‐displaced Acehnese children. Using multivariate linear regression models, it was found that displacement distance was a consistent predictor of impairment using the Brief Impairment Scale. Exposure to tsunami‐related trauma markers was not significantly linked with impairment in the model. Paternal employment was a consistent protective factor for child functioning. These findings suggest that post‐disaster displacement and the subsequent familial economic disruption are significant predictors of impaired functioning in children's daily activities. Post‐disaster interventions should consider the disruption of familiar environments for families and children when relocating vulnerable populations to avoid deleterious impacts on children's functioning.     

Early signs of recovery of Acropora palmata in St. John,US Virgin Islands

Since the 1980s, diseases have caused significant declines in the population of the threatened Caribbean coral Acropora palmata. Yet it is largely unknown whether the population densities have recovered from these declines and whether there have been any recent shifts in size-frequency distributions toward large colonies. It is also unknown whether colony size influences the risk of disease infection, the most common stressor affecting this species. To address these unknowns, we examined A. palmata colonies at ten sites around St. John, US Virgin Islands, in 2004 and 2010. The prevalence of white-pox disease was highly variable among sites, ranging from 0 to 53 %, and this disease preferentially targeted large colonies. We found that colony density did not significantly change over the 6-year period, although six out of ten sites showed higher densities through time. The size-frequency distributions of coral colonies at all sites were positively skewed in both 2004 and 2010, however, most sites showed a temporal shift toward more large-sized colonies. This increase in large-sized colonies occurred despite the presence of white-pox disease, a severe bleaching event, and several storms. This study provides evidence of slow recovery of the A. palmata population around St. John despite the persistence of several stressors.     

Effect of impeller type and agitation on the performance of pilot scale ASBR and AnSBBR applied to sanitary wastewater treatment

The objective of this work was to assess the effect of agitation rate and impeller type in two mechanically stirred sequencing batch reactors: one containing granulated biomass (denominated ASBR) and the other immobilized biomass on polyurethane foam (denominated AnSBBR). Each configuration, with total volume of 1 m³, treated 0.65 m³ sanitary wastewater at ambient temperature in 8-h cycles. Three impeller types were assessed for each reactor configuration: flat-blade turbine impeller, 45°-inclined-blade turbine impeller and helix impeller, as well as two agitation rates: 40 and 80 rpm, resulting in a combination of six experimental conditions. In addition, the ASBR was also operated at 20 rpm with a flat-blade turbine impeller and the AnSBBR was operated with a draft tube and helix impeller at 80 and 120 rpm. To quantify how impeller type and agitation rate relate to substrate consumption rate, results obtained during monitoring at the end of the cycle, as well as the time profiles during a cycle were analyzed. Increasing agitation rate from 40 rpm to 80 rpm in the AnSBBR improved substrate consumption rate whereas in the ASBR this increase destabilized the system, likely due to granule rupture caused by the higher agitation. The AnSBBR showed highest solids and substrate removal, highest kinetic constant and highest alkalinity production when using a helix impeller, 80 rpm, and no draft tube. The best condition for the ASBR was achieved with a flat-blade turbine impeller at 20 rpm. The presence of the draft tube in the AnSBBR did not show significant improvement in reactor efficiency. Furthermore, power consumption studies in these pilot scale reactors showed that power transfer required to improve mass transfer might be technically and economically feasible.     

NON-FUEL MINERALS AND THE INTEGRATED PROGRAMME FOR COMMODITIES

The paper reviews progress in the individual commodity negotiations for non-fuel minerals covered by the Integrated Programme for Commodities (IPC). Given the minimal progress to date, the author reconsiders the objectives of the IPC to gauge the extent to which they meet the needs of countries engaged in mineral production or trade. It is suggested that the major focus of the IPC be towards price stability, with less attention to other aspects of market instability and other market problems. For this reason, the IPC discussions have failed to focus on such major issues of concern in the mineral sector as measures necessary to guarantee adequate supplies of minerals over the long-term, the financing of the required investments, the expansion of production by developing countries and the securing of price levels sufficient to generate appropriate returns on capital employed. Cet article est consacré à l'étude des progrès accomplis en matière de négociations individuelles sur les produits de base concernant les minéraux non-combustibles qui figurent au Programme intégré pour les produits de base. Etant donné les résultats peu concluants qui ont été enregistrés, ‘auteur examine à nouveau les objectifs du programme pour évaluer jusqu’ à quel point ils répondent aux besoins des pays engagés dans la production ou le commerce de minéraux. Selon cet article, ‘accent principal du programme a été mis sur’ instabilité en matière de prix au détriment d'autres aspects de 'instabilité du marché et d'autres problèmes connexes. De telles discussions ont éludé les points d'intérét majeurs du secteur minier, à savoir, les mesures nécessaires à assurer à long terme des approvisionnements suffisants en minéraux, le financement des investissements nécessaires, une participation plus active des pays en développement à 'expansion de la production et le maintien de niveaux de prix susceptibles de créer des profits suffisants sur le capital investi. Este artículo revisa los progresos alcanzados en la negociación individual de minerales no combustibles cubiertos por el programs ‘Integrated Programme for Commodities’ (IPC). Dado el progreso mínimo presente, el autor reconsidera los objectivos de la IPC para apreciar el grado en que estos objectivos se ajustan a las necesidades de los países dedicados a la producción y comercio de minerales. Se sugiero que el IPC enfoca su acción a la estabilidad de precios, con menor atención a otros aspectos de inestabilidad y otros problemas de mercado. Por esta razón las discuciones de IPC no han considerado asuntos de tanta importancia en el sector minero tales como las medidas para garantizar suministros adecuados de minerales por largos plazos, el financimiento de las inversiones requeridas, la expansión de la producción por los países en desarollo y la garantía de niveles de precios suficientes para generar retornos adecuados del capital invertido.     

Classification of Ephemeral,Intermittent, and Perennial Stream Reaches Using a TOPMODEL‐Based Approach

Whether a waterway is temporary or permanent influences regulatory protection guidelines, however, classification can be subjective due to a combination of factors, including time of year, antecedent moisture conditions, and previous experience of the field investigator. Our objective was to develop a standardized protocol using publically available spatial information to classify ephemeral, intermittent, and perennial streams. Our hypothesis was that field observations of flow along the stream channel could be compared to results from a hydrologic model, providing an objective method of how these stream reaches can be identified. Flow‐state sensors were placed at ephemeral, intermittent, and perennial stream reaches from May to December 2011 in the Appalachian coal basin of eastern Kentucky. This observed flow record was then used to calibrate the simulated saturation deficit in each channel reach based on the topographic wetness index used by TOPMODEL. Saturation deficit values were categorized as flow or no‐flow days, and the simulated record of streamflow was compared to the observed record. The hydrologic model was more accurate for simulating flow during the spring and fall seasons. However, the model effectively identified stream reaches as intermittent and perennial in each of the two basins.     

Air quality measurements for the aerosol research and inhalation epidemiology study

Measurements of pollutant gases, airborne particulate matter mass and composition, and meteorology have been made at a core site near downtown Atlanta, GA, since August 1998 in support of the Aerosol Research and Inhalation Epidemiology Study (ARIES). This site is one of eight in the Southeastern Aerosol Research and Characterization network. The measurement objective is to provide a long-term, multivariate dataset suitable for investigating statistical associations of respiratory and cardiovascular disease with airborne particulate matter composition, meteorology, and copollutant gases through epidemiologic modeling. Measurements are expected to continue through 2010. Ancillary multiyear measurements at additional sites in the Atlanta metropolitan area and in short-term exposure assessments have been used to estimate the exposure/measurement error associated with using data from a central site to approximate human exposures for the entire area. To date, 13-, 25-, and 53-month air quality datasets have been used in epidemiologic analyses.