Terrestrische ökotoxikologische Testmethoden für die Tropen

Goal, Scope, and Background

Soil organisms play a crucial role in the terrestrial ecosystem. Plant Protection Products (PPPs) are known to affect soil organisms and might have negative impacts on soil functions influenced by these organisms. Little research has been done to day on the impact of PPs on tropical ecosystems. Therefore, in this study it was investigated whether fate and effects of pesticides differ between tropical and temperate regions and whether data generated under temperate conditions can be used for the Environmental Risk Assessment (ERA) in tropical regions.

Methods

In the first part of this study, the effects of two fungicides (Benomyl and Carbendazim) and one insecticide (lambda-Cyhalothrin) on soil invertebrates (i.e. earthworms and arthropods) were evaluated in laboratory tests modified for tropical conditions (temperature, soil, test species). Besides using some native species, the tests were done mainly with two (temperate and tropical) strains of earthworms (Eisenia fetida) and the peregrine isopod speciesPorcellionides pruinosus as standard test species. The chemicals were spiked in two natural and two artificial soils. A tropical artificial soil (TAS), containing a tropical fern product (Xaxim) or coconut coir dust as organic matter, was developed in this study.

Results and Conclusions

The results from the laboratory tests showed that all three test chemicals differed from those gained under temperate conditions. In the case of the fungicides the toxicity was lower but in the case of the insecticide higher under tropical than under temperate conditions. The native tropical earthwormPontoscolex corethrurus reacted more sensitively against Carbendazim in comparison to the standard test speciesEisenia fetida.

Recommendation and Perspective

Details of the environmental risk assessment of the three model chemicals based on the results of the laboratory described here (and including the results of higher tier tests (semi-field and field tests)) will be described in Part 2 of this series     

Short-term population dynamics of Tisbe cucumariae (Copepoda: Harpacticoida)

Population dynamics of a benthic harpacticoid copepod (Tisbe cucumariae) were studied in a seawater-system holding tank during fall-spring, 1979/1980, and January, 1981. These populations were opoortunistic scavengers, especially on the dead bodies of Ciona intestinalis, a solitary tunicate. Copepod population densities, life-history stage structures, sex ratio and percentages of females with egg sacs were determined in the absence or in the presence of dead tunicates (from death through dissolution to final disappearance). Taken together, all the data are consistent with a hypothesis for food-dependent cycling of populations of T. cucumariae through the following 4 density states: (1) a maintenance state of low density, which is the usual condition during the absence of a concentrated food source; (2) a colonization state of moderate density, which results from attraction and immigration to a new food source; (3) a bloom state at the food source, where a high density results from intensive reproduction combined with a tendency of all life stages to remain at the site; (4) a dispersal state of moderate density as the copepods emigrate from the site of the consumed food. Evidence is also presented for a facultative slowing of naupliar development in T. cucumariae in the absence of a concentrated food source such as a dead tunicate. Possible advantages afforded by delayed development are discussed in terms of risk-spreading in a stochastically hostile environment.     

Numerical modeling of simultaneous tracer release and piscicide treatment for invasive species control in the Chicago Sanitary and Ship Canal,Chicago, Illinois

In December 2009, during a piscicide treatment targeting the invasive Asian carp in the Chicago Sanitary and Ship Canal, Rhodamine WT dye was released to track and document the transport and dispersion of the piscicide. In this study, two modeling approaches are presented to reproduce the advection and dispersion of the dye tracer (and piscicide), a one-dimensional analytical solution and a three-dimensional numerical model. The two approaches were compared with field measurements of concentration and their applicability is discussed. Acoustic Doppler current profiler measurements were used to estimate the longitudinal dispersion coefficients at ten cross sections, which were taken as reference for calibrating the longitudinal dispersion coefficient in the one-dimensional analytical solution. While the analytical solution is fast, relatively simple, and can fairly accurately predict the core of the observed concentration time series at points downstream, it does not capture the tail of the breakthrough curves. These tails are well reproduced by the three-dimensional model, because it accounts for the effects of dead zones and a power plant which withdraws nearly 80 % of the water from the canal for cooling purposes before returning it back to the canal.     

Transfer of natural and anthropogenic radionuclides to ants, bryophytes and lichen in a semi-natural ecosystem

Few data are available to quantify the transfer of both natural and anthropogenic radionuclides to detritivorous invertebrates to facilitate estimation of the internal dose to such biota in models used to assess radiation exposure. To enhance the available data, activity concentrations of ¹³⁷Cs, ⁴⁰K, ⁹⁰Sr, ^239?+?240Pu, ²⁴¹Am, ²³⁵U and ²³⁸U were measured in ants (Formicidae) and corresponding undisturbed soil collected from the Zlatibor mountain in Serbia and ant/soil concentration ratios (CR) calculated. The ²⁴¹Am concentration ratios for ants were fourfold higher than those calculated for ants in a previous study whereas they are similar to the more numerous data previously reported for a range of detritivorous invertebrates in other studies. CR values for ¹³⁷Cs in ants were similar to the few other reported values and slightly lower than those for a range of detritivorous invertebrates. Those for ^239?+?240Pu were slightly higher than those for ants in two other studies but they were close to upper limit of a range of data reported for detritivorous invertebrates. All the CR values will be included in a future revision of the ERICA Tool database and will particularly improve the information available for uranium.     

Estimating life cycle greenhouse gas emissions from corn–ethanol: a critical review of current U.S. practices

This study reviews corn–ethanol life cycle assessment studies and modeling tools to identify key discrepancies between studies, and shortcomings in analysis of greenhouse gas emissions. The primary conclusions are that direct land use change modeling, soil N₂O emissions modeling, and co-product crediting were either poorly treated or treated inconsistently across most or all studies. Despite the existence of improved methods and tools proposed by institutional bodies and the broader research community, the state of the practice tends towards over-simplified and highly generalized methods that increase potential uncertainty in greenhouse gas estimates and prevents differentiation among producers of corn ethanol.     

Modelling the Choice Between Multiple-Use vs. Specialised Forest Management and its Impact on Forest Management Costs

Forests provide ecosystem services, including timber production. In some cases, private forest owners perform management actions in order to enhance the provision of such services, or they may be encouraged by public policies and payments. This paper focuses specifically on the decision to set forest land aside for biodiversity conservation, as part of the more general question of the efficiency of multiple-use vs. specialised management of forest lands. We propose an econometric analysis to identify factors in the set-aside choice and measure the impact of this decision on forest management costs. The results show that the set-aside choice depends on the landowners’ income and motivations related to forest amenities. The choice of specialised management, as reflected in the set-aside decision, has a significant and positive impact on the intensity of management in the remainder of the forest and on management costs. From a policy implications point of view, these results suggest that instruments such as forest certification, subsidies and reverse auctions for conservation will be most likely to attract the interest of those forest owners with personal motivations and forest properties that offer both benefit and cost advantages in opting for such specialised management strategies.

     

Human Bocavirus: Detection,Quantification and Molecular Characterization in Sewage and Surface Waters in Uruguay

Human bocavirus (HBoV) infections are related to respiratory and gastroenteric diseases. The aim of this study was to investigate the presence of HBoV in both sewage and surface waters in Uruguay. Sixty-eight sewage samples from the cities of Salto, Paysandú, Bella Unión, Fray Bentos, Treinta y Tres and Melo and 36 surface water samples from the cities of Salto, Florida and Santa Lucía were studied. HBoV was screened by multiplex qPCR for the detection of the four subtypes, followed by monoplex qPCRs for the independent quantification of each subtype. A qualitative PCR followed by DNA sequencing and phylogenetic analysis was carried out for molecular characterization of HBoV strains. HBoV was present in a high frequency (69%) in sewage and only one positive sample (3%) was found in surface water. Concerning sewage samples, HBoV1 was detected in 11 (23%) out of the 47 positives samples, with a mean concentration of 8.2 × 10⁴ genomic copies/Liter (gc/L), HBoV3 was detected in 35 (74%) of the positive samples with a mean concentration of 4.1 × 10⁶ gc/L and subtypes 2 and/or 4 were detected in 39 (83%) of the positive samples with a mean concentration of 7.8 × 10⁶ gc/L. After the phylogenetic analysis performed by a Bayesian approach, the four HBoV subtypes were confirmed. This is the first study determining a high frequency of HBoV and the presence of the four HBoV subtypes in aquatic matrices in Latin America, mainly in sewage. Although HBoV was scarcely detected in surface water, a waterborne transmission is likely to occur if people enter in contact with polluted surface waters for recreational activities such as fishing or swimming since an elevated frequency of HBoV was detected in raw sewage which is usually directly discharged into surface waters.     

Modeling the Impacts of Two Bark Beetle Species Under a Warming Climate in the Southwestern USA: Ecological and Economic Consequences

Predicted climate warming is expected to have profound effects on bark beetle population dynamics in the southwestern United States. Temperature-mediated effects may include increases in developmental rates, generations per year, and changes in habitat suitability. As a result, the impacts of Dendroctonus frontalis and Dendroctonus mexicanus on forest resources are likely subject to amplification. To assess the implications of such change, we evaluated the generations per year of these species under three climate scenarios using a degree-day development model. We also assessed economic impacts of increased beetle outbreaks in terms of the costs of application of preventative silvicultural treatments and potential economic revenues forgone. Across the southwestern USA, the potential number of beetle generations per year ranged from 1–3+ under historical climate, an increase of 2–4+ under the minimal warming scenario and 3–5+ under the greatest warming scenario. Economic benefits of applying basal area reduction treatments to reduce forest susceptibility to beetle outbreaks ranged from 7.75/ha (NM) to7.75/ha (NM) to 95.69/ha (AZ) under historical conditions, and 47.96/ha (NM) to47.96/ha (NM) to 174.58/ha (AZ) under simulated severe drought conditions. Basal area reduction treatments that reduce forest susceptibility to beetle outbreak result in higher net present values than no action scenarios. Coupled with other deleterious consequences associated with beetle outbreaks, such as increased wildfires, the results suggest that forest thinning treatments play a useful role in a period of climate warming.