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991.
992.
M. D. Vazquez-Illanes R. Barcia I. Ibarguren J. A. Villamarín J. I. Ramos-Martínez 《Marine Biology》1992,112(2):277-281
6-phosphofructo-2-kinase (PFK-2) from the mantle of the sea musselMytilus galloprovincialis Lmk, collected from the Ría de Arosa (NW Spain) in 1990, was purified 550-fold by extraction and sequential affinity chromatography on Affi-gel Blue and ATP-agarose columns. The enzyme was a dimer with a native molecular weight of 100 kilodaltons (KDa) and a subunitM
r of 53 KDa. PFK-2 activity is dependent on the presence of Pi. At physiological Pi concentrations, the enzyme exhibits hyperbolic kinetics with both ATP and Fru-6-P, withK
m values of 0.62 and 0.37 mM respectively. In vivo, PFK-2 activity is limited by the concentration of Fru-6-P which is low in comparison with theK
m for this substrate. Citrate and PEP inhibited PFK-2 activity. 相似文献
993.
The organization, general anatomy, and surface microanatomy of all regions of the gills of a representive bivalve mollusc, Placopecten magellanicus Gmelin, were studied using stereo-microscopic, histological, and scanning electron microscopic techniques. Individuals were collected in May and November 1985 from Chamcook Bay, New Brunswick, Canada. In addition to correcting earlier accounts of this structure, a number of new observations are reported. The orientation of the ciliated spurs appears to be responsible for the sinusoidal arrangement of the gill filaments. Micrographs showing the structure of the dorsal respiratory expansion are presented. The entire abfrontal surface of the principal filament, including the dorsal respiratory expansion, is densely ciliated and mucosecretory. These characteristics may aid in the establishment of a respiratory current and in the prevention of gill damage during escape responses. All nonciliated regions of the gill filaments are covered with microvilli, thus greatly increasing the surface area of the gill. The feeding mechanism is discussed in relation to the dorsal and ventral ciliated tracts. Symbiotic ciliate protozoans are constantly dislodged from the gill filaments and transported via the ventral mucus string to the buccal region. The nutritional implications of these observations are discussed. 相似文献
994.
Photosynthesis and respiration in Ahnfeltia plicata (Huds.) Fries (Gigartinales) was measured in a seawater flowthrough system at different temperatures, salinities and photon flux densities (PFD). The exchanges of dissolved oxygen and inorganic carbon were continuously recorded with an oxygen probe and a pH electrode measuring variation in CO2–HCO
3
-
equilibrium as pH changes. Highest apparent photosynthesis at moderate photon flux density (PFD 50 E m-2 s-1) was found at 15°C and 33 S. Photosynthesis was measured up to PFD 500 E m-2 s-1 and no light saturation was documented. In the present experimental set-up, with continuous supply of fresh seawater, the number of limiting factors during photosynthesis measurements is reduced. 相似文献
995.
Mesopelagic fish were collected from a cruise with the R.V. “Dr. Fridtjof Nansen” to the northern Red Sea and the interior Gulf of Aden off the Republic of Djibouti in March 1981. Off the Republic of Djibouti five species of mesopelagic fishes were caught and in the Red Sea six species. Benthosema pterotum (Alcock) were dominant in both areas. In the Red Sea Maurolicus muelleri (Gmelin) were also abundant. The mesopelagic fish caught in the Red Sea showed a pattern of vertical migration similar to that observed in other areas, in spite of the special hydrographical regime. The number of gill rakers of B. pterotum caught in the Red Sea differed significantly from that of all other populations studied. The number of photophores of M. muelleri apparently do not differ much from other populations. B. pterotum from the Red Sea mature at a larger size than other populations, while M. muelleri mature at a smaller size. The relative fecundity of M. muelleri is higher in the Red Sea than in other regions studied, while no significant difference was observed in B. pterotum. Both species appear to be opportunistic feeders. Their feeding chronology in the Red Sea seems to be similar to those observed in other areas. 相似文献
996.
997.
Hugh?DrummondEmail author Cristina?Rodríguez Adriana?Vallarino Claudia?Valderrábano Gabriela?Rogel Emilio?Tobón 《Behavioral ecology and sociobiology》2003,53(5):287-296
According to the desperado sibling hypothesis, chicks of obligately siblicidal species kill their junior broodmates as early as possible because junior broodmates face dire ecological prospects and are consequently predisposed to aggressively overthrow elder broodmates by all-out aggression. The agonistic behavior of junior chicks is virtually unknown because they die so young. We investigated the agonistic behavior of hypothetical desperados by fostering junior brown boobies (Sula leucogaster, an obligately siblicidal species) into nests of the blue-footed booby (S. nebouxii, a facultatively siblicidal species) containing an older singleton blue-foot chick. Controls were junior blue-footed boobies fostered into the same situation. Junior brown boobies were 7 times more aggressive than controls and most of them dominated their elder and larger nestmates. Four of nine brown booby juniors showed relentless aggression, delivering up to 711 pecks, bites and pushes (including "expulsion pushes") per hour, thereby overwhelming nestmates 90% heavier and permanently expelling one of them from the nest. Similarly, in natural broods of two surviving brown booby chicks, the losing chick was 13 times as aggressive as blue-foot subordinates, up to at least age 7 weeks. Contrast of the two species of booby suggests the evolution of agonistic roles within broods may be partly driven by selection on potential victims to express a level and type of aggressiveness appropriate to their status-related ecological prospects. 相似文献
998.
Copulatory plugs do not assure high first male fertilisation success: sperm displacement in a lizard
Pedro L. Moreira Vera L. Nunes José Martín Octávio S. Paulo 《Behavioral ecology and sociobiology》2007,62(2):281-288
Sperm competition selects for opposing male defensive and offensive reproductive traits, and its outcome may be determined
by the effectiveness to which one trait has evolved to out-compete the other. We tested the effectiveness of a first male
plug physical interference with a second male insemination (defence) vs the effectiveness of plug and associated sperm displacement
by a second male (offence) on the outcome of sperm competition in Iberian rock lizards. We conducted a double mating experiment
where we compared the proportion of eggs per clutch fertilised by the same second males (against the same first males) when
they copulated with females 30 min (first male plug adhered firmly inside the female cloaca) and 4 h (first male plug loosely
adhered or shed from the female cloaca) after first males. We found that second males fertilised the majority of the eggs
per clutch in the 30-min treatment, whereas fertilisations were equally shared between the two males in the 4-h treatment.
These results show that plugs have little defensive effectiveness, and thus, do not assure high first male fertilisation success.
Instead, sperm displacement appears to be associated with plug displacement. That is, because sperm embedded in first male
plugs, and displaced from competition for fertilisations by second males, is expected to increase in number with decreasing
time allowed for female sperm transport, second males thus enjoy higher fertilisation success. This study shows that offensive
plug displacement out-competes plug defensive role in Iberian rock lizards. Moreover, it reveals sperm displacement as a novel
sperm competition mechanism in reptiles. 相似文献
999.
1000.
Brian L. Murphy Amy P. Toole Paul D. Bergstrom 《Environmental geochemistry and health》1989,11(3-4):163-169
This paper describes risk assessment methods for two chronic exposure pathways involving arsenic contaminated soil, namely inhalation of fugitive dust emissions over a lifetime, and inadvertent soil/house dust ingestion. The endpoint in the first case is assumed to be lung cancer and in the second case skin cancer. In order to estimate exposures, inhalation rates and soil/dust ingestion rates are estimated for different age groups; indoor/outdoor time budgets for different age groups are developed; and indoor surface dust and air arsenic concentrations are estimated based on outdoor concentration measurements. Differences observed in indoor/outdoor ratios and arsenic containing dust particle size among different types of communities are noted, as well as possible relationship of particle size to bioavailability. Calculations of risk are presented using cancer potency factors developed by the U.S. Environmental Protection Agency, and uncertainties in these toxicity estimates are described based on: (1) evidence that arsenic may be neither a cancer initiator nor promotor, but may act instead as a late stage carcinogen and (2) evidence that the arsenic dose-response relationship for ingestion may be nonlinear at low doses due to increasing methylation of inorganic arsenic. The first of these considerations influences the relative importance ascribed to arsenic doses in different age groups. The latter consideration indicates that the risk estimates described here are probably very conservative. 相似文献