Combination effects of anticholinesterasics in acetylcholinesterase of a fish species: effects of a metallic compound,an organophosphate pesticide,and a pharmaceutical drug

The simultaneous presence of distinct compounds in the aquatic environment can be causative of various toxicological interactions. This scenario challenges ecotoxicologists, since the assessment of toxicological effects caused by the simultaneous presence of multiple substances is by far more complicated. An illustrative example can be given by mentioning the anticholinesterasic compounds: by studying the level of cholinesterase impairment of an exposed organism, it is possible to ascertain the level of exposure to all anticholinesterasics (despite their chemical classes and natures) that the organism was subjected to. In this work, we describe the effects of three chemically different, albeit mechanistically, and toxicologically similar compounds (copper, chlorfenvinphos, and pyridostigmine) on cholinesterases of the fish Gambusia holbrooki. The results showed that the combinatorial effects may be of considerable extent, even for levels of exposure that are close to the ones already reported in the wild, for each isolated compound.     

Effects of environmentally relevant concentrations of metallic compounds on the flatfish Scophthalmus maximus: biomarkers of neurotoxicity,oxidative stress and metabolism

Flatfish species, such as the turbot (Scophthalmus maximus), are common targets for toxic effects, since they are exposed through the food chain (ingestion of contaminated preys) and are in direct contact with the waterborne contaminant and sediments. Furthermore, these fish species live in close proximity to interstitial water that frequently dissolves high amounts of contaminants, including metals. Despite this significant set of characteristics, the present knowledge concerning flatfish contamination and toxicity by metals is still scarce. To attain the objective of assessing the effects of metals on a flatfish species, S. maximus specimens were chronically exposed to lead, copper and zinc, at ecologically relevant concentrations, and biochemical (oxidative stress: catalase and glutathione S-transferases activities, and lipid peroxidation; neurotoxicity: cholinesterase activity) parameters were assessed on selected tissues (gills and liver). Copper had no significant effects on all tested parameters; lead was causative of significant increases in liver GSTs activities and also in lipoperoxidation of gill tissue; exposure to zinc caused a significant increase in catalase activity of gill tissue. None of the tested metals elicited noteworthy effects in terms of neurotoxicity. The obtained results showed that only the metal lead is of some environmental importance, since it was able to cause deleterious modifications of oxidative nature at relevant concentrations.     

Biochemical effects of acetaminophen in aquatic species: edible clams Venerupis decussata and Venerupis philippinarum

Acetaminophen (paracetamol) is one of the most used pharmaceutical drugs, due to its antipyretic and analgesic properties that turn it into a primary choice in varied pathologies and conditions. However, and despite its massive use, acetaminophen is not exempt of adverse effects, especially when administered in over dosage, which are related to the formation of toxic metabolites by oxidative pathways. It is thus possible to observe that toxicity caused by acetaminophen is usually mediated by reactive oxygen species and can result in multiple effects, ranging from protein denaturation to lipid peroxidation and DNA damage. The occurrence of acetaminophen has been reported in the aquatic environment, being important to address the potential exertion of toxic effects on nontarget environmentally exposed organisms. The present study intended to characterize the effects of acute acetaminophen exposure on physiological traits (antioxidant defense, oxidative damage) of two species of bivalves, namely, the edible clams Venerupis decussata and Venerupis philippinarum. Results showed a significant increase in all oxidative stress biomarkers, evidencing the bioactivation of acetaminophen into a deleterious prooxidant, triggering the onset of deleterious effects. Furthermore, strong interspecific differences were observed among responses of the two tested species, which was a major issue due to intrinsic ecological implications when one considers that both species share the same habitat.     

Validation of a dynamic model of hydrogen permeation through Pd-based membranes

Pd-based membranes have been studied for pure hydrogen separation from syngas: in particular, a mathematical model of a Pd membrane for hydrogen separation has been developed.This model can be used in process and assessment studies of the parameters which characterize the mass transfer phenomena (such as: hydrogen permeability, surface coverage and limiting step). By coupling the permeation and water gas shift reaction kinetics, it can also be used to evaluate the performances of the membrane reactor. Further, it can be helpful to evaluate the best assembly and sizing of a H₂/CO₂ separation system.The model takes into account the kinetics of H₂ adsorption/desorption on Pd surface, the H₂ permeation into the palladium bulk and in the porous layer, and the kinetics of CO, CO₂, H₂O, O₂, H₂S competitive adsorption/desorption on Pd surface. It is also comprehensive of flux equations and bulk mass, momentum and energy balance.The results released by the model were compared to the experimental data during both the transient phase and the steady state conditions. A satisfactory agreement between model and experimental data was found.     

Vacuum pyrolysis process for remediation of hydrocarbon-contaminated soils

A vacuum pyrolysis process was used for the remediation of hydrocarbon–contaminated soils from “Les Vidangeurs de Montréal” site in Mascouche in the province of Québec, near Montréal. Ten samples were tested on a laboratory scale batch reactor, and one sample was tested on a prototype process development unit. The process is simple, efficient, reliable, and economically competitive with other existing technologies. The vacuum pyrolysis process efficiently treated soils contaminated with a variety of pollution levels and types, irrespective of the soil matrix, providing treated soils meeting the A criterion (noncontaminated, residential level) of the Ministry of Environment Québec (MENVIQ). The pyrolytic oil and the noncondensable pyrolytic gases can be used as makeup fuel in the process, because they have a high calorific value and their combustion should not pose any emission problems. The waterphase effluent must be treated before discharge.     

International survey on the education of occupational health nurses

In 1990 the Scientific Committee on Occupational Health Nursing (SCOHN) of the International Commission on Occupational Health (ICOH) carried out a survey on the education of occupational health nurses (OHN) in various countries. A questionnaire with 23 questions was mailed to 50 countries.Replies were received form altogether 37 countries (74%). The length of the general nursing education varies from two to five years, being three years in 21 countries. In two European countries it is less than three years.Some special training in occupational health is included in the general nursing education in 14 countries. However, the length of this training varies considerably. The special training is the longest in Denmark, Finland and Norway.According to the answers, special postgraduate courses are organized in 21 countries. Training in occupational health is also included in the public health nursing education of 12 countries.University level programs on occupational health nursing are available in eight countries. Joint education on occupational health at university level has been organized for various professionals in 13 countries. The length of this education varies greatly from some months to several years. In four countries the length of this joint education is one academic year.General nursing education is the basic requirement for occupational health nurses in most of the countries surveyed. However, training in occupational health is not included in this education in most of the countries. Special postgraduate courses in occupational health are organized in 21 countries to OHNs who are employed in Occupational Health Services. There is an urgent need to develop the education of OHNs in most countries surveyed.     

Demographic factors and land-use planning in the small islands of Southern Europe

Since the end of the 1970s, the southern European countries have shown an exceptional reduction in fertility rate. From the highest levels among the developed nations, these countries dropped beneath the substitution rate level: in Greece there is an average of about 1.5 children per woman, and Italy (starting three to four years ago), with 1.3 children per woman, is now the country with the lowest fecundity rate in the world. Land-use planning in southern European small islands therefore requires substantial revision. In the areas where western civilization began, which are highly populated and have a long history, cultural and ethnic aspects of tradition are fundamental to environmental management and to the defense of historical heritage. They also place a strong value on sustaining tourism, the most relevant economic activity, that allows them to survive and maintain a high welfare level. For some decades they have had populations with a marked presence of young people and high emigration rates, but now they are fast becoming dominated by the elderly and must prepare for a period of fast reduction in youth of the workforce, while the peripheral areas of Asia and Africa are entering a sudden demographic growth phase. The demographic structure has also been deeply altered both by previous migrations and by random variations, as usually happens in all small communities. Social services for younger and older people have had to be adapted rapidly, reorganizing high-school management, hospital and health-care structures, in-house assistance, and so on. There is a need to rethink the job market and favor the immigration of highly specialized workers, which is a necessity for technical evolution. Sustainable development is constrained nowadays not only by the scarcity of natural resources, but also by the quality and quantity of human resources. Proper policies for population and land-use planning are highly correlated factors; they have to be considered with respect of these new, rapid demographic changes.     

A study about remedial measures to reduce 222Rn concentration in an experimental building

Radon gas emanating from underground can spread to adjoining closed areas. It can concentrate and reach levels which represent a risk to people's health. It is well known that radon presence in most areas depends mainly on the area's geological features. Indoor radon concentrations further depend on the type of structure, construction materials and the technology used for the building. Therefore, indoor radon monitoring is of primary importance for deciding whether remedial measures are to be adopted for reducing harmful concentrations. This approach has been tried by measuring radon concentration in an experimental building situated in Milan (Italy). This building situated in a geological area that is considered at low radon risk. The results were obtained after analysing radon concentration in indoor rooms, crawl spaces, soil gas and in the atmosphere outside and by measuring before and after adoption of remedial measures. The study shows that improper building design can give rise to higher indoor radon accumulation even in an area of poor radon exhalation. Furthermore, the results enable quantification of the effectiveness of the remedial measures.     

Forest Perception and Knowledge of Hikers and Mountain Bikers in Two Different Areas in Northwestern Switzerland

Recreational activities can have major impacts on vegetation and wildlife in frequently visited forests. We assessed forest perception and knowledge (state, functions, and species diversity) among hikers and mountain bikers in a frequently visited, seminatural suburban recreation forest (Muttenz) and in a more distantly situated, naturally grown excursion forest (Wasserfallen) in northwestern Switzerland. In all, 239 hikers and 126 mountain bikers were interviewed. Mountain bikers in both forests and hikers in the more intensely used recreation forest at Muttenz assessed the state of the forest less optimistically and showed a higher awareness of the negative impact of recreational activities on the flora and fauna than hikers at Wasserfallen. Furthermore, mountain bikers seemed aware of the social conflicts caused by their activity, since they appreciated neutral or positive encounters with other forest visitors. In contrast, 57% of hikers at Muttenz reported on negative experiences with other forest visitors, particularly with mountain bikers. In general, the interviewees ecological and biological forest knowledge (forest type and function, species diversity) was rather high. A large proportion was aware of the pros and cons of different forest conditions for plants and animals, and could name or recognize at least some plant and/or animal species typical for the visited forest. The forest knowledge was neither influenced by the type of recreational activity carried out nor by any aspect of forest visit behavior (frequency and duration of forest visits, means of transportation and travel distance to forest). However, the interviewees forest knowledge was influenced by their age and educational level.