载体吸附培养黄孢原毛平革菌(Phanerochaete chrysosporium)和云芝(Trametes versicolor)对染料的脱色

比较研究了不同载体吸附培养的黄孢原毛平革菌(Phanerochaete chrysosporium)和云芝(Trametes versicolor)对染料连续脱色的效果。结果表明:(1)白腐真菌黄孢原毛平革菌和云芝分别在含有球形的木屑、玉米芯和花生壳载体的液体环境中振荡培养,菌体以膜状或团状形式大量附着生长在载体表面。(2)连续4轮脱色过程中,不论黄孢原毛平革菌还是云芝,都是木屑为载体的培养液的持续脱色和产酶能力最好,宜选择木屑为载体。其中,木屑为载体的黄孢原毛平革菌培养液经过2轮连续12d脱色后对活性黑RB5的最高脱色率仍能达到97%,在第3轮脱色中对活性红M-3BE的最终脱色率接近96%,并且能产生最高611U/L的锰依赖过氧化物酶(MnP)和1 477U/L的木质素过氧化物酶(LiP)。(3)在实际应用中应该将木屑为载体的黄孢原毛平革菌和云芝培养液都投加到含染料废水处理系统中,强化生物处理效果。     

浅谈突发溢油事件应急处置技术及装置的应用

概述了海洋溢油应急处置的技术方法,分析了溢油应急处置装置设备的应用特点,为海洋溢油应急处置工作提供理论依据。     

基于演化博弈的流域生态补偿利益冲突分析

资源合理利用与环境保护是维护当今经济平稳、健康、较快发展的重要前提。生态补偿是缓解经济快速发展与环境污染、生态环境破坏之间利益矛盾冲突的重要手段。它是为了制止生态破坏而征收或补偿的费用,所以不同于一般排污费和资源补偿费。本文根据补偿的类型不同,将生态补偿分为资源型生态补偿和环境型生态补偿。本文以流域生态补偿为例,将流域上游政府的策略简化为保护或不保护河流,下游政府针对上游的情况,选择"自主型"或"接受型",运用演化博弈的方法,分析流域生态补偿的特点和结果。结果表明:流域生态补偿需要中央政府的适度干预,而且关键点是保证上游地方政府收益始终最大化;中央政府干预的程度是使上游政府群的收益在保护的情况下比不保护的收益要大,而干预的结果则是使流域上下游的(保护,补偿)策略成为演化博弈的长期稳定均衡。最后本文针对我国现行流域管理体制,为我国流域生态补偿提供了政策建议,以推广我国生态补偿政策。     

参与式灌溉管理对农户用水行为的影响

参与式灌溉管理的核心在于保障农业高效、公平用水和农业灌溉的可持续发展.本文通过对甘肃省张掖灌区用水户协会参与灌溉管理制度对农户用水行为影响的实证分析结果表明,用水户协会的成立、排灌系统的完善、农业生产结构的调整和水权市场的建立等因素,在一定程度上提高了农户灌溉水的利用效率,缓解了张掖灌区水资源短缺的局面.然而,由于当前用水者协会"自上而下"的行政管理导致用水户在灌溉管理中并不具有真正的发言权和决策权,用水户对灌溉管理的民主参与并没有达到节约用水的目标.为此,我国应建立完善用水户参与灌溉管理的激励机制和灌溉管理中的民主决策机制,使协会成员真正参与到灌溉工程的建管、灌溉水价的制定和剩余水权的流转和分配中来,保证农户通过参与灌溉管理获得更为可观的效益,是我国未来用水户协会灌溉管理制度改革的一项重要内容.     

Real-time reverse transcription PCR analysis of trichloroethylene-regulated toluene dioxygenase expression in Pseudomonas putida F1

Toluene dioxygenase (tod) is a multicomponent enzyme system in Pseudomonas putida F1. Tod can mediate the degradation of Trichloroethylene (TCE), a widespread pollutant. In this study, we try to explore the TCE-regulated tod expression by using real-time qRT-PCR. The minimal culture media were supplemented with glucose, toluene, or a mixture of glucose/toluene respectively as carbon and energy sources. The TCE was injected into each medium after a 12-hour incubation period. The TCE injection severely affected bacterial growth when cultured with toluene or toluene/glucose mixtures. The cell density dropped 61 % for bacteria growing in toluene and 36 % for bacteria in the glucose/toluene mixture after TCE injection, but the TCE treatment had little effect on bacteria supplied with glucose alone. The decrease in cell number was caused by the cytotoxicity of the TCE metabolized by tod. The results from the real-time qRT-PCR revealed that TCE was capable of inducing tod expression in a toluene-dependent manner and that the tod expression level increased 50 times in toluene and 3 times in the toluene/glucose mixture after 6 hours of TCE treatment. Furthermore, validation of the rpoD gene as a reference gene for P. putida F1 was performed in this study, providing a valuable foundation for future studies to use real-time qRT-PCR in the analysis of the P. putida F1 strain.     

曝气对两种人工湿地污水净化效果的影响

采用复合垂直流和水平潜流2种人工湿地处理混合污水,研究了在气水比(曝气体积/湿地进水体积)为2、4、6、8和10时2种人工湿地对污水的净化效果.结果显示:(1)复合垂直流人工湿地以气水比为2进行曝气时,对污水的净化效果最好,上行池出水的COD、TN、NH4+-N、NO3--N平均去除率比不曝气时分别提高了16.47%、...     

日本发展低碳经济的政策体系综述

发展低碳经济已经成为许多国家和区域发展的重要策略。本文总结了日本发展低碳经济的背景、目标和基本理念,综述了其促进低碳经济发展的政策体系框架和主要内容,具体包括低碳能源、财政、技术、交通、绿色消费、信息公开、国际合作、宣传教育等政策措施,指出日本低碳经济政策体系中存在的不足之处,并提出我国促进低碳经济发展的政策建议。     

我国各省市报废汽车量预测

根据统计数据,采用加权平均法计算我国汽车平均使用年限为12年;根据各省市1998—2008年汽车新增消费量统计数据,推测得到2010—2020年各省市汽车报废量;分析了报废汽车中载货车、客车和轿车结构特征和报废汽车东、中、西部区域分布特征。     

Increased urinary 8-hydroxy-2��-deoxyguanosine levels in workers exposed to di-(2-ethylhexyl) phthalate in a waste plastic recycling site in China

Background

Di-(2-ethylhexyl) phthalate (DEHP) is a common plasticizer used in industrial and diverse consumer products. Animal studies indicate DEHP caused developmental, reproductive, and hepatic toxicities. However, human studies of the potential effects of DEHP are limited.

Methods

The exposed site with a history of over 20 years of waste plastic recycling was located in Hunan Province, China. The reference site without known DEHP pollution source was about 50 km far away from the exposed site. In this study, 181 workers working in plastic waste recycling and 160 gender?Cage matched farmers were recruited. DEHP concentrations in water and cultivated soil samples, serum thyroid-stimulating hormone, malondialdehyde (MDA), superoxide dismutase (SOD), urinary 8-hydroxy-2??-deoxyguanosine (8-OHdG), and micronuclei frequency in human capillary blood lymphocytes were analyzed.

Results

Mean levels of DEHP were greater in environment at the recycling site than at reference site (industry wastewater for the exposed: 42.43 ??g/l; well water: 14.20 vs. 0.79 ??g/l, pond water: 135.68 vs. 0.37 ??g/l, cultivated soil: 13.07 vs. 0.81 mg/kg, p?p?p?Conclusions The occupational DEHP exposure might contribute to oxidative deoxyribonucleic acid damage in the male workers.