Modified CDKN2B (p15) and CDKN2A (p16) DNA methylation profiles in urban pesticide applicators

Environmental Science and Pollution Research - Gene-specific changes in DNA methylation by pesticides in occupationally exposed populations have not been studied extensively. Of particular concern...     

Rapid and Low-Cost Method for Evaluation of Nutrient Release from Controlled-Release Fertilizers Using Electrical Conductivity

Methods to determine nutrient release rates of coated fertilizers usually rely on chemical analyses, which often are time-consuming and/or expensive. Our goal was to develop an innovative and rapid low-cost method to evaluate nutrient release from polymer coated MAP or urea using conductometry. The release in water is determined by measuring the electric conductivity (EC) over time, with intervals dependent on release rates. In the case of soluble salt fertilizers, EC can be immediately determined and converted to a concentration using a calibration curve. In the case of urea, an additional step is needed to convert the neutral urea into ammonium. The release rates in water were assessed for a range of commercial and laboratory-coated fertilizers. A validation test demonstrated strong agreement with the release determined using analytical techniques. The EC method hence offers an easy way to quickly evaluate the time course of release of nutrients from controlled-release fertilizers.     

Valuing diversity: a group‐value approach to understanding the importance of organizational efforts to support diversity

Using Leventhal's rules as well as the group‐value model of procedural justice, we first examined how the negative effects of perceived racial discrimination on procedural justice judgments can be attenuated by perceived organizational efforts to support diversity. Secondly, we examine how these effects ultimately impact affective commitment and organizational citizenship behavior. We found that employees who believe some individuals in the workplace are discriminating against them on the basis of race tend to report lower levels of procedural justice from the organization. However, this negative relationship was attenuated when employees perceived that their organization was making efforts to support diversity. Results suggest that individuals' perceptions of organizational efforts to support diversity can help restore perceptions of procedural justice for employees who experience racial discrimination at work. Improving procedural justice also positively impacts affective commitment and organizational citizenship behavior directed at the organization. Copyright © 2009 John Wiley & Sons, Ltd.     

Influence of salinity and temperature on the activity of biosurfactants by polychaete-associated isolates

Influence of different parameters on biosurfactant (BS) activity was carried out on strains that were isolated from the polychaetes Megalomma claparedei, Sabella spallanzanii and Branchiomma luctuosum and additional 30 strains that were previously identified as potential BS producers from crude oil enrichments of the same polychaete specimens. The selection of BS-producing strains from polychaete natural samples was carried out by using standard screening tests. The BS activity by each isolate was evaluated for the effect of salinity and temperature on emulsion production and surface tension reduction, during incubation in mineral medium supplemented with tetradecane or diesel oil. All isolates showed a similar time course of BS activity, and the latter was more influenced by salinity rather than temperature. Some of the BS producers belonged to genera that have not (i.e. Citricoccus, Cellulophaga, Tenacibaculum and Maribacter) or have poorly been (Psychrobacter, Vibrio, and Pseudoalteromonas) reported as able to produce BSs. This is remarkable as some of them have previously been detected in hydrocarbon-enriched samples. Results confirm that filter-feeding polychaetes are an efficient source for the isolation of BS producers.     

Photocatalytic degradation of microcystin-LR in aqueous solutions

In this work, the photocatalytic degradation of aqueous microcystin-LR was studied using TiO₂ and ZnO as photocatalysts. The process was optimised and characterised at the bench scale (200 mL); both semiconductors exhibited a high degradation capacity at reaction times of 1 min (degradation greater than 95%). The transient species that were observed indicate that the degradation occurs via the multiple hydroxylation and elimination of the labile peptide residues of the molecule. When photocatalysis was applied in a continuous treatment system (20–50 L), the photocatalytic process exhibited a high degradation efficiency, which resulted in residual microcystin-LR concentrations that were less than 1 μg L^?1 (C₀ = 5 μg L^?1).     

Does seston size structure reflect fish-mediated effects on water quality in a degraded semiarid wetland?

This study evaluates whether the size structure of seston (the sum of living and nonliving particles in the water column) reflects the effects of fish on wetland water quality. Using enclosures, we measured water quality and zooplankton community structure in the presence and absence of two fish species with distinct foraging strategies [benthivorous carp (Cyprinus carpio) and planktivorous mosquitofish (Gambusia holbrooki)]. Seston collected from the enclosures was counted and sized automatically with a Coulter counter, and the size structure in the range of 1–60 μm was modelled using the underlying Pareto distribution of particles. Only Cyprinus contributed to a loss of water quality (increased nutrient levels, algal and non-algal turbidity, hypoxia), while both fish species changed zooplankton community composition compared to fishless controls. However, these changes were not reflected in parameters [goodness of fit (r ²) and parameter c (slopes)] of Pareto models. Multivariate statistics suggest that seston size structure responded more to environmental gradients related to water depth but the relationships were also weak. Our overall result contrasts with the regulation of size spectra constructed from living organisms. Although seston integrates many structural and functional features of the water column, the lack of strong relationships between Pareto model parameters and water quality suggests that a size-based approach to characterise seston structure has a limited potential for assessing biota-mediated effects in wetlands in a straightforward manner.     

A Simplified Dynamic Model for the Activated Sludge Process with High Strength Wastewaters

A simplified dynamic model for the activated sludge process with high-strength wastewaters is presented. The model is based on activated sludge models and predicts effluent chemical oxygen demand concentration using only four parameters. Experimental data for testing the model were obtained in an activated sludge plant using flax retting wastewater. The proposal of the model is described: mass balance to the system, selection of variables and behaviour of the theoretical values versus experimental results.     

Attitudes and Perceptions about Ecological Resources, Hazards, and Future Land Use of People Living near the Idaho National Engineering and Environmental Laboratory

This paper examines the attitudes and perceptions of local people about ecological resources, environmental hazards, and future land use of the Idaho National Engineering and Environmental Laboratory (INEEL). Such monitoring of attitudes is an important aspect of environmental assessment. We interviewed 262 people who attended the 42nd Annual Free Fishermen's Breakfast at St. Anthony, Idaho, on 23 March 1997. We tested the null hypotheses that there were no differences in perceptions about ecological resources, hazards and future land use of INEEL as a function of gender, age, education, and place of residence. Dumping trash in the ocean and cutting rainforests rated as the environmental problems of highest concern, and ozone, radon and high voltage lines rated the lowest; cleaning up Department of Energy sites rated intermediate. Respondents were most willing to expend government funds to make drinking water clean. Three significant differences were found: 1) women rated environmental problems as more severe than did men, and women were more willing to expend federal funds to solve these problems, 2) respondents under 30 years of age rated environmental problems as more severe than older people, and they were more willing to spend money to solve them, and, 3) respondents who had not finished high school were more concerned with environmental problems and were more willing to spend money to solve them than respondents with a high school education. Maintaining INEEL as a National Environmental Research Park (NERP) rated as the most preferred future land use, followed by continued reprocessing of nuclear materials and hunting. Using INEEL for housing and additional nuclear waste storage rated the lowest. Men rated grazing livestock and additional nuclear waste storage higher than did women. In general, respondents from 30 – 49 rated several economic uses (hunting, grazing livestock, growing crops) higher than did people in other age groups. Respondents with some college rated these economic uses higher than did respondents who had not graduated from high school. These results indicate that respondents living around INEEL believe that INEEL should continue with a reprocessing and NERP mission, but that other peripheral uses, such as hunting, hiking and grazing, should be allowed on some of the land. These views should aid in environmental assessment of the site, and in developing further management plans for INEEL.     

Recovery of Francisella tularensis from soil samples by filtration and detection by real-time PCR and cELISA

The aim of this study was to develop a specific and highly sensitive method able to detect very low concentrations of Francisella tularensis in soil samples by real-time PCR (qPCR) with SYBR Green I. tul4 gene, which encodes the 17-kDa protein (TUL4) in F. tularensis strains, was amplified using a LightCycler (LC) device. We achieved a detection limit of 0.69 fg of genomic DNA from F. tularensis subp. holarctica live vaccine strain (LVS), corresponding to a value less than 3.4 genome equivalents per reaction. The qPCR was shown to be specific, highly sensitive and reproducible. In addition, we evaluated 2 new methods for recovering bacteria from soil based on 1-step filtration using glass fiber filters and PVDF filters. These filtration methods enabled us to recover F. tularensis efficiently from soil samples. As few as 50 CFU per 0.5 g of soil were detected by qPCR. Capture enzyme-linked immunosorbent assay (cELISA) allowed us to detect and quantify the amount of bacteria recovered from soil by an immunological method. Although qPCR was more sensitive than cELISA, we did not observe substantial differences in the amount of bacteria quantified by both methods.     

Cholinesterase characterization in Corbicula fluminea and effects of relevant environmental contaminants: a pesticide (chlorfenvinphos) and a detergent (SDS)

This study characterizes the cholinesterasic forms present in the bivalve Corbicula fluminea, through the analysis of differential affinity towards various substrates and inhibitors. This approach also permitted the validation of cholinesterase inhibition in the mentioned species for monitoring purposes by evaluating the inhibitory activity of a detergent (SDS) and a pesticide (chlorfenvinphos), both in vitro and in vivo. The cholinesterasic form present in the tissues of C. fluminea showed intermediate properties in terms of its relation with substrates and inhibitors. Furthermore, it was not inhibited by the detergent SDS under any of the test conditions, but was clearly inhibited by the selected organophosphate. The data show clearly that the main cholinesterasic form in C. fluminea is responsive to the organophosphate chlorfenvinphos, thus supporting the use of this biomarker for the environmental monitoring of specific contaminants such as anticholinesterasic pesticides. Despite not being commonly attained in the wild, the levels of chlorfenvinphos required to elicit a significant ChE inhibition were only one order of magnitude above actual values of aquatic contamination by this pesticide.