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M. A. Garrido-Ramos M. C. Soriguer R. de la Herrán M. Jamilena C. Ruiz Rejón A. Domezain J. A. Hernando M. Ruiz Rejón 《Marine Biology》1997,129(1):33-39
Morphometric and genetic methods were used to identify two sturgeon species, Acipenser naccarii Bonaparte, 1836, and A. sturio Linnaeus, 1758, captured in some of the principal rivers of the Iberian Peninsula, including the Guadalquivir. After measuring
25 Iberian specimens from a fishery and several Spanish and Portuguese museums and applying stepwise discriminant analysis
(SDA), four specimens preserved in different museums [two specimens from the Guadalquivir river (EBD-8173 and EBD-8174), one
specimen from the Tagus river (MUC1) and one specimen from the Mondego river (MUC46B)], as well as five specimens captured
in the Guadalquivir river in the 1940s but not preserved (CM1, CM2, CM3, CM4 and CM5), were identified as A. naccarii. After cloning and characterisation of a satellite-DNA family, HindIII, from A.␣naccarii genome, its absence from the genome of A.␣sturio was determined. Using this satellite-DNA as a genetic marker and by means of dot-blotting, we demonstrate that the DNA of
the two specimens captured during the mid-1970s in the Guadalquivir river cross-hybridised with HindIII satellite-DNA sequences of A.␣naccarii. We conclude that A. naccarii is autochthonous to the Iberian Peninsula and is not, as was previously believed, endemic to the Adriatic Sea.
Received: 28 November 1996 / Accepted: 10 March 1997 相似文献
355.
Sardine (Sardina pilchardus) spawning seasonality in European waters of the northeast Atlantic 总被引:3,自引:0,他引:3
Yorgos Stratoudakis Stephen Coombs Ana Lago de Lanzós Nicholas Halliday Gersom Costas Bruno Caneco Concha Franco David Conway M. Begoña Santos Alexandra Silva Miguel Bernal 《Marine Biology》2007,152(1):201-212
Egg data from ichthyoplankton monitoring sites in the western English Channel (1988–2003) and northern Spain (1990–2000) and
macroscopic maturity data from biological samples of purse seine landings in western and southern Iberia (1980–2004) are used
to describe the spawning seasonality of sardine (Sardina pilchardus) in European waters of the northeast Atlantic using generalised additive models. The fitted models reveal a double peak in
spawning activity during early summer and autumn in the western Channel, a wider spring peak off northern Spain and a broad
winter season in the western and southern Iberian Peninsula. At all sites, a high probability of spawning activity was observed
over at least 3 months of the year, with the duration of the season increasing with both decreasing latitude and increasing
fish size. Off western and southern Iberia there are indications that the spawning season has been of longer duration in recent
years for all size classes (reaching in some cases 8 months of the year for large fish). These patterns are in general agreement
with existing literature and theoretical expectations of sardine spawning being driven locally by the seasonal cycle of water
temperature, assuming preferences for spawning at 14 –15°C and avoidance for temperatures below 12°C and above 16°C. Regional
quotient plots indicated that spawning tolerance to higher temperatures increases progressively with decreasing latitude.
Despite the weak evidence for geographical differences in temperature tolerance that may have some genetic origin, the degree
of spatio-temporal overlap in sardine-spawning activity within Atlantic European waters is unlikely to promote any reproductive
isolation in that area. 相似文献
356.
The spermatozoa of the genus Odiomaris Ng and Richer de Forges, 1996 (=Amarinus Lucas, 1980) have the components typical of eubrachyuran (Heterotremata + Thoracotremata) sperm, but differ significantly
from all other investigated eubrachyurans in at least ten characteristics: (1) presence of an epiopercular dome; (2) separation
of all but the central region of the operculum from the remainder of the acrosome by an infra-opercular rim; (3) the fact
that the acrosome is smaller in volume than the nucleus; (4) the acrosome is strongly emergent from the nucleus, being surrounded
only basally by nuclear material; (5) the cytoplasmic sheath, ending anteriorly with the nucleus, is also basal; (6) division
of the acrosome contents into an inner and outer acrosome zone is scarcely apparent in longitudinal section as the inner zone
is narrow and of doubtful homology; (7) the thin, putative inner acrosome zone is anteriorly almost septate owing to several
longitudinal corrugations; (8) basally there is a unique “fringe zone”; (9) the acrosome, including the epinuclear dome, is
longer than wide; (10) the unique helical and posterolateral disposition of the nuclear arms. From a purely spermatological
viewpoint, Odiomaris (as exemplified by O. pilosus and O. estuarius), and provisionally the Hymenosomatidae, are thus excluded from the Thora- cotremata, in which they were formerly placed,
nor are they readily placeable in the Heterotremata.
Received: 30 December 1996 / Accepted: 4 February 1997 相似文献
357.
358.
姚蓉 《应用与环境生物学报》1996,(1)
用一组多克隆抗体对马氏甲烷八叠球菌(Methanosarcinamazei)S-6菌株的基因组DNA文库进行了筛选.仅选出p60A克隆能对马氏甲烷八叠球菌中可发生细胞形态学变化菌株的抗血清发生阳性反应.对p60A克隆的双链DNA进行了序列分析.识别出一开式阅读框架(openreadingframeP,ORFP).表达的蛋白是ORFP编码的蛋白的3倍,并得到ORFP蛋白的三聚体,在SDS-PAGE中表现出整体蛋白的迁移行为.同时,此表达蛋白抗10%SDS,6mol/L尿素及热处理.基因结构分析表明,ORFP具有与M.barkrimcrA有同源性的核糖体结合位点和一个与甲烷细菌启动子共有序列相似度达77%的启动子序列.使用参照序列分析表明,由ORFP演绎的氨基酸序列,具有高密度的带电荷的氨基酸和占优势的β-层迭构型.对此表达蛋白作了Neurosroracrassa的porin蛋白抗血清试验,以研究其可能功能.检验了M.mazeiS-6细胞的蔗糖梯度制备物,对此原细胞中的ORFP蛋白作了定位.结果表明,ORFP蛋白可能是一种古细菌Porin,其在M.mazeiS-6中的表达,可能象大肠杆菌那样与渗透压调节有关. 相似文献
359.
Calcification rates in different fragments along branches of the hermatypic coral Stylophora pistillata were tested in the laboratory using a new technique, the optic glassfiber method. By this method, the tested colony remains constantly in dark conditions while a narrow beam of light, transferred through the optic fiber, illuminates a small distinct point of coral tissue (on a branch tip or base). The selected illuminated portion of the branch serves as the experimental fragment, while all the other parts of the same colony serve as the dark controls. The results indicate that significantly more calcium is incorporated in the tip fragments than in the bases, both in light and in dark conditions (4.1 to 13.2 times more). Illumination of the tips or the bases did not stimulate or enhance the calcification rates of these fragments. Thus, in all colonies tested, the calcification rates of the illuminated fragments were not significantly different from the average rates of other similar, non-illuminated fragments of the same colony. It is suggested that light does not directly enhance calcification in hermatypic corals, but rather, that light enhances O2 production, which consequently stimulates coral metabolism. Our preliminary results indicate that calcification rates recorded in aerated dark experiments are significantly higher than calcification rates of non-aerated dark controls. 相似文献
360.
S.?A.?PouletEmail author M.?Richer de Forges A.?Cueff J.-F.?Lennon 《Marine Biology》2003,143(5):889-895
Two double-labelling methods, Tunel+propidium iodide and Annexin V-FITC+propidium iodide, have been tested to diagnose cell degradation processes in N1–N2 nauplius stages produced by spawning females of the copepod Calanus helgolandicus fed either non-toxic (PRO: control) or toxic (TR1) diets under laboratory conditions. Observation of labelled samples with a confocal laser-scanning microscope revealed that the maternal-food effect, following absorption of the toxic diet, induced cell apoptosis and necrosis in 80–100% of the offspring, which sooner or later died. Similar cell damages were also observed in 18–50% of the nauplii produced by females incubated in filtered seawater. Such cell degradation processes and high mortality were not observed in nauplii produced by females fed the non-toxic diet. Our protocols allowed detection of cell degradations before death of unhealthy larvae, preferentially with a confocal microscope or a conventional visible-fluorescent-light microscope.Communicated by O. Kinne, Oldendorf/Luhe 相似文献