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71.
Rapid interphase analysis for prenatal diagnosis of translocation carriers using subtelomeric probes
Mark J. Pettenati Chris Von Kap-Herr Bethy Jackle Peggy Bobby Philip Mowrey Stuart Schwartz P. Nagesh Rao Jon Rosnes 《黑龙江环境通报》2002,22(3):193-197
Interphase fluorescence in situ hybridization (FISH) has become an accepted laboratory technique for the rapid and preliminary prenatal assessment of chromosome aneuploidy. The introduction of subtelomeric FISH probes now allows for the molecular–cytogenetic analysis of terminal chromosome rearrangements. In a prospective study, we examined the prenatal use of subtelomeric probes on interphase cells to rapidly detect the carrier status of a fetus when a parent carried a known reciprocal or Robertsonian chromosome translocation. Three of the cases were identified as being abnormal. All cases were confirmed by routine cytogenetic analysis. These findings clearly demonstrated the utility of this technique and these probes to rapidly and correctly identify balanced and unbalanced chromosome anomalies of a fetus that could result from parental translocations. Copyright © 2002 John Wiley & Sons, Ltd. 相似文献
72.
Beekman M Good G Allsopp MH Radloff S Pirk CW Ratnieks FL 《Die Naturwissenschaften》2002,89(10):479-482
In the Cape honey bee Apis mellifera capensis, workers lay female eggs without mating by thelytokous parthenogenesis. As a result, workers are as related to worker-laid eggs as they are to queen-laid eggs and therefore worker policing is expected to be lower, or even absent. This was tested by transferring worker- and queen-laid eggs into three queenright A. m. capensis discriminator colonies and monitoring their removal. Our results show that worker policing is variable in A. m. capensis and that in one colony worker-laid eggs were not removed. This is the first report of a non-policing queenright honey bee colony. DNA microsatellite and morphometric analysis suggests that the racial composition of the three discriminator colonies was different. The variation in policing rates could be explained by differences in degrees of hybridisation between A. m. capensis and A. m. scutellata, although a larger survey is needed to confirm this. 相似文献
73.
This research has demonstrated that even in a small urbanised country like Britain, communities can still be remote. The paper analyses the nature of remote settlements and suggests that beyond the rural idyll, there are deprived communities. In order to obtain real data, a study area in the North Pennines was selected. The types of remote communities were evident throughout the study area and one of each type was selected for further study. It became clear that villages with an industrial base had most potential, due to high energy demand, vulnerability and community involvement. A village with a high measure of multiple deprivation and suffering from fuel poverty was chosen for a more detailed investigation. Energy demand profiles were developed and candidate technologies nominated. The latter were tested by filters that assessed fitness for application, robustness and autonomy. The most appropriate combinations of technologies were proposed. The residents and their community representatives were involved in the process and recognised the benefits of the proposals to themselves and others. 相似文献
74.
Chris Riedy Jennifer Kent Nivek Thompson 《Journal of Environmental Planning and Management》2019,62(1):151-171
Effective environmental governance requires institutional change. While some actors work to change institutions, others resist change by defending and maintaining institutions. Much of this institutional work is ‘meaning work’, which we define as the practice of crafting, adapting, connecting and performing meanings to purposively create, maintain or disrupt institutions. This paper constructs a concept of meaning work that highlights agency in carrying meanings across scales and between discursive layers, while noting the structuring role of prevailing discourses. It grounds the concept using two environmental governance cases at very different scales: a local democratic innovation employed by Noosa Council in Queensland, Australia; and the international campaign to divest from fossil fuels. The cases demonstrate the diversity of meaning work and the difficulty of achieving deep discursive change. They point to the need for environmental governance practitioners to rework existing meanings to construct compelling stories for change, taking advantage of narrative openings. 相似文献
75.
76.
Chris Pratt Adrian S. Walcroft Julie Deslippe Kevin R. Tate 《Waste management (New York, N.Y.)》2013,33(2):412-419
Landfills that generate too little biogas for economic energy recovery can potentially offset methane (CH4) emissions through biological oxidation by methanotrophic bacteria in cover soils. This study reports on the CH4 oxidation efficiency of a 10-year old landfill cap comprising a volcanic pumice soil. Surface CH4 and CO2 fluxes were measured using field chambers during three sampling intervals over winter and summer. Methane fluxes were temporally and spatially variable (?0.36 to 3044 mg CH4 m?2 h?1); but were at least 15 times lower than typical literature CH4 fluxes reported for older landfills in 45 of the 46 chambers tested. Exposure of soil from this landfill cover to variable CH4 fluxes in laboratory microcosms revealed a very strong correlation between CH4 oxidation efficiency and CH4/CO2 ratios, confirming the utility of this relationship for approximating CH4 oxidation efficiency. CH4/CO2 ratios were applied to gas concentrations from the surface flux chambers and indicated a mean CH4 oxidation efficiency of 72%. To examine CH4 oxidation with soil depth, we collected 10 soil depth profiles at random locations across the landfill. Seven profiles exhibited CH4 removal rates of 70–100% at depths <60 cm, supporting the high oxidation rates observed in the chambers. Based on a conservative 70% CH4 oxidation efficiency occurring at the site, this cover soil is clearly offsetting far greater CH4 quantities than the 10% default value currently adopted by the IPCC. 相似文献
77.
78.
Wendy A. Williams Mark E. Jensen J. Chris Winne Roland L. Redmond 《Environmental monitoring and assessment》2000,64(1):105-114
Accurate delineation and characterization of valley-bottom settings is crucial to the assessment of the biological and geomorphological components of riverine systems; yet, to date, most valley-bottom mapping endeavors have been done manually. To improve this situation, we developed automated techniques in a Geographic Information System (GIS) for delineating and characterizing valley-bottom settings in river basins ranging in size from approximately 1,000–10,000 km2. All procedures were developed with ARC/INFO GIS software and fully automated in Arc Macro Language (AML). The GRID module is required for valley-bottom delineation and slope calculations; whereas characterization (i.e., measuring the width of the valley-bottom zone) requires Coordinate Geometry (COGO) in the ARCEDIT module. The process requires three inputs: a polygon coverage of the analysis area; an arc coverage of its hydrography, and a grid representing its digital elevation. The AML is designed to operate within a wide range of computer memory/disk space options, and it allows users to customize several procedures to match the scale and complexity of a given analysis area with available computer hardware. 相似文献
79.
Sebastiaan van Herk Jeroen Rijke Chris Zevenbergen Richard Ashley Broos Besseling 《Journal of Environmental Planning and Management》2015,58(3):554-575
Adaptive co-management and learning are paramount for integrated flood risk management. Relevant literature focuses on adaptation at the level of physical and societal systems. The level of projects and programmes is largely overlooked, but they comprise interventions that adapt our physical systems and they provide opportunities for learning to contribute to transitions of societal systems. This paper aims to increase understanding on how learning takes place and can be stimulated within a programme. The mixed-method case study of Room for the River, a €2.3 billion programme for flood risk management, shows that a programme can be organised using various governance arrangements to stimulate learning and be a means for adaptive co-management to deliver upon environmental objectives. 相似文献
80.
Judy Lawrence Frances Sullivan Alison Lash Gavin Ide Chris Cameron Lisa McGlinchey 《Local Environment》2015,20(3):298-320
Adaptation to climate change has been reviewed in several developed nations, but in none where consideration of the effects of climate change is required by statute and devolved to local government. We examine the role of institutional arrangements, the players operating under them, the barriers and enablers for adaptation decision-making in the developed nation of New Zealand. We examine how the roles and responsibilities between national, regional and local governments influence the ability of local government to deliver long-term flexible responses to changing climate risk. We found that the disciplinary practices of law, engineering and planning, within legal frameworks, result in the use of static mechanisms which create inflexible responses to changing risk. Several enablers are identified that could create greater integration between the different scales of government, including better use of national policy instruments, shared professional experience, standardised information collection and risk assessment methods that address uncertainties. The framing of climate risk as dynamic and changing that differentiates activities over their lifetime, development of mechanisms to fund transitions towards transformational change, are identified as necessary conditions for delivering flexible responses over time. 相似文献