Sorption of polar and nonpolar aromatic compounds to four surface soils of eastern China

Improved predictions on the fate of organic pollutants in surface environments require a better understanding of the underlying sorption mechanisms that control their uptake by soils. In this study, we monitored sorption of nine aromatic compounds with varying physicochemical properties (hydrophobicity, electron-donor/acceptor ability and polarity), including two polycyclic aromatic hydrocarbons, two chlorobenzenes, two nitroaromatic compounds, dichlobenil, carbaryl and 2,4-dichlorophenol in aqueous suspension of four surface soils of eastern China. The tested soils were characterized with respect to organic carbon (OC) content, black carbon content, mineralogy, morphology and size fraction to assess the role of the diverse soil characteristics in sorption. The results of this study show that not only the solute hydrophobicity and the OC content of soil are important to the retention of organic pollutants, but also the solute molecular structure and the soil nature.     

Modulation of steroidogenesis by coastal waters and sewage effluents of Hong Kong, China, using the H295R assay

BACKGROUND, AIM, AND SCOPE: The presence of a variety of pollutants in the aquatic environment that can potentially interfere with the production of sex steroid hormones in wildlife and humans has been of increasing concern. The aim of the present study was to investigate the effects of extracts from Hong Kong marine waters, and influents and effluents from wastewater treatment plants on steroidogenesis using the H295R cell bioassay. After exposing H295R cells to extracts of water, the expression of four steroidogenic genes and the production of three steroid hormones were measured. MATERIALS AND METHODS: Water samples were collected during the summer of 2005 from 24 coastal marine areas and from the influents and effluents of two major waste water treatment plants (WWTPs) in Hong Kong, China. Samples were extracted by solid phase extraction (SPE). H295R cells were exposed for 48 h to dilutions of these extracts. Modulations of the expression of the steroidogenic genes CYP19, CYP17, 3betaHSD2, and CYP11beta2 were determined by measuring mRNA concentrations by real-time polymerase chain reaction (Q-RT-PCR). Production of the hormones progesterone (P), estradiol (E2), and testosterone (T) was quantified using enzyme linked immunosorbent assays (ELISA). RESULTS: Extracts from samples collected in two fish culture areas inhibited growth and proliferation of H295R cells at concentrations greater or equal to 10(5) L equivalents. The cells were exposed to the equivalent concentration of active substances in 10,000 L of water. Thus, to observe the same level of effect as observed in vitro on aquatic organisms would require a bioaccumulation factor of this same magnitude. None of the other 22 marine samples affected growth of the cells at any dilution tested. Twelve of the marine water samples completely inhibited the expression of CYP19 without affecting E2 production; inhibition of CYP17 expression was observed only in one of the samples while expression of CYP11beta2 was induced as much as five- and ninefold after exposure of cells to extracts from two locations. The expression of the progesterone gene 3betaHSD2 was not affected by any of the samples; only one sample induced approximately fourfold the production of E2. Although more than twofold inductions were observed for P and T production, none of these values were statistically significant to conclude effects on the production of these two hormones. While influents from WWTPs did not affect gene expression, an approximately 30% inhibition in the production of E2 and a 40% increase in P occurred for the exposure with influents from the Sha Tin and Stonecutters WWTPs, respectively. Effluents from WWTPs did not affect the production of any of the studied hormones, but a decrement in the expression of the aldosterone gene CYP11beta2 was observed for the Sha Tin WWTP exposure. No direct correlation could be established between gene expression and hormone production. DISCUSSION: Observed cytotoxicity in the two samples from fish culture areas suggest the presence of toxic compounds; chemical analysis is required for their full identification. Although effluents from WWTPs did not affect hormone production, other types of endocrine activity such as receptor-mediated effects cannot be ruled out. Interactions due to the complexity of the samples and alternative steroidogenic pathways might explain the lack of correlation between gene expression and hormone production results. CONCLUSIONS: Changes observed in gene expression and hormone production suggest the presence in Hong Kong coastal waters of pollutants with endocrine disruption potential and others of significant toxic effects. The aromatase and aldosterone genes seem to be the most affected by the exposures, while E2 and P are the hormones with more significant changes observed. Results also suggest effectiveness in the removing of compounds with endocrine activity by the WWTPs studied, as effluent samples did not significantly affect hormone production. The H295R cell showed to be a valuable toll in the battery required for the analysis of endocrine disrupting activities of complex environmental samples. RECOMMENDATIONS AND PERSPECTIVES: Due to the intrinsic complexity of environmental samples, a combination of analytical tools is required to realistically assess environmental conditions, especially in aquatic systems. In the evaluation of endocrine disrupting activities, the H295R cell bioassay should be used in combination with other genomic, biological, chemical, and hydrological tests to establish viable modes for endocrine disruption and identify compounds responsible for the observed effects.     

Fenton法氧化处理水中土霉素的研究

为了评价Fenton法预处理抗生素生产废水中残留的高浓度土霉素的可行性,对Fenton试剂(Fe2 -H2O2)法催化氧化降解水溶液中的土霉素进行了研究.探讨了H2O2与Fe2 投加比例、投加量以及起始pH值对降解效果的影响,同时考察了土霉素废水中大量共存的草酸根离子对氧化过程的影响.结果表明,当H2O2与Fe2 按照5:1的比例投加时降解效率最高,最佳初始pH值在3.0-4.0之间.在最佳投加比例下,H2O2投加量为0.9 mmol/L,100 mg/L的土霉素可在10 min内得到完全降解.最佳反应条件下经过处理后土霉素水溶液TOC的去除率达40%左右,可生化性(BOD5/COD)也得到明显的改善.水中共存的草酸根离子对氧化效率具有明显的影响,但通过增加亚铁离子的量可以消除草酸的影响.     

Rhodobacter sp. NP25b菌株缺氧降解壬基酚聚氧乙烯醚的研究

从城市污水处理厂活性污泥中分离得到一株能够在缺氧条件下以壬基酚聚氧乙烯醚(NPEOs)为惟一碳源和能源生长的菌株NP25b.经生理生化鉴定和16S rRNA基因序列分析,该菌株属于红细菌属(Rhodobacter sp.),对该菌株降解NPEOs的特性进行了研究.结果表明,在缺氧条件下,菌株NP25b在7 d内对初始底物浓度为400 mg/L NPEOs的降解率可达84%.利用液相色谱-质谱(LC-MS)和气相色谱-质谱(GC-MS)对NPEOs降解中间产物进行了分析,结果表明,主要降解产物为短链NPEOs和壬基酚聚氧乙烯醚乙酸(NPECs),其中包括具有较强内分泌干扰效应的NP1EO.该菌株能够代谢含有疏水基团的聚氧乙烯醚类表面活性剂,例如辛基酚聚氧乙烯醚和脂肪醇聚氧乙烯醚.推测菌株NP25b降解NPEOs是通过乙氧基(EO)链末端氧化后逐步切割完成的.     

压力式接触氧化法同步硝化反硝化脱氮性能研究

研究了压力式接触氧化法的脱氮性能,分析了容积负荷、溶解氧和停留时间等因素对反应器脱氮效果的影响.研究表明,压力式接触氧化法具有明显的同步硝化反硝化现象,当HRT=1.8 h时,DO高达5.4 mg/L,可获得90%以上的反硝化率.当HRT=1.8 h,溶解氧4～5 mg/L,容积负荷为10～12 kg COD/(m3·d)时,氨氮去除率80%左右,总氮去除率达70%～80%.     

二次热解吸-气相色谱-质谱分析室内挥发性有机化合物

采用二次热解吸-气相色谱.质谱法对室内空气进行了定量和定性分析,共检出挥发性有机物245种,包括烷烃、烯烃、芳香类化合物、卤代烃、醇、醛、酮、酯、醚等化合物,住宅类室内空气中挥发性有机化合物浓度平均值明显高于办公类室内这些物质的浓度平均值,对室内空气样品分析中的特例进行了可能的污染源解析,推测室内过量使用液体胶粘剂有可能是引起污染物严重超标的原因之一.     

错流式生物滴滤床净化甲苯废气

采用焦化厂污泥为菌源驯化甲苯降解菌,接种错流式生物滴滤床,净化含甲苯废气。研究了生物滴滤床的挂膜启动和长期运行情况,填料和营养液对滴滤床去除能力的影响,并对长期运行的压降进行了观察分析。反应器挂膜启动需要6 d时间,稳定运行的平均去除效率为95％,单位体积最大去除负荷为251 g/(m³·h)。结果表明,采用错流式生物滴滤床可以有效去除甲苯废气;以比表面积大的生物陶粒作为填料以及定期适量更换营养液,均有助于提高生物滴滤床的去除能力;错流式生物滴滤床具有压降小、气液分布均匀的特点。     

Pb/Zn冶炼废渣中重金属的生物浸出-盐浸处理

利用中温嗜热菌对某铅锌冶炼废渣进行生物浸出盐浸处理研究,并根据国家固体废物浸出毒性方法(HJ/T299-2007)对盐浸后余渣进行毒性分析。研究结果表明,在pH 1.5、温度65℃、矿浆浓度5%的优化条件下生物浸出3 d后,废渣中Cu、In、Ga和Zn的浸出率分别达到了91.5%、91.8%、84.9%和93.4%;盐浸生物浸出渣,其浸出液中Ag、Pb浓度分别为7.6和247.5 mg/L,可从废渣中有效回收Cu、In、Ga、Zn、Ag和Pb。生物浸出盐浸处理后余渣约为原渣量的70%;毒性分析浸出液中重金属元素Ag、As、Cd、Cu、Pb和Zn浓度分别为2～3.5、2～3、0.3～0.5、30～50、2～4、20～60 mg/L,低于国家危险废物鉴别标准(GB5085.3-2007)。根据试验结果,提出了针对冶炼废渣资源化、减量化、无害化的生物浸出盐浸联用工艺。     

高压热水液化厨余垃圾的可行性研究

以高压热水为反应媒体处理厨余垃圾,得到液、气、固3种产物,并用GC-MS、GC-TCD和元素分析的方法对产物进行了表征。结果表明该技术处理厨余垃圾在实现减量化（达90.6%）和无害化的同时,可以达到资源化的目的。得出最优反应条件为：反应温度400℃,压力21.2 MPa,反应时间为40 min,优化的条件下固、液、气3种产物收率分别为21.5%、55.0%和5.2%。此外,对主要典型产物的形成机理进行了探讨。     

强化混凝处理城市纳污河污水运行过程中搅拌能量的影响

介绍了采用混凝技术大规模处理运河污水时所遇到的问题,并通过混凝试验分析了问题的原因。提出并初步探讨了混凝搅拌能量及其计算方式,利用混凝搅拌能量参数分析混凝反应效果的差异,说明在实际生产中混凝反应过程需要有足够的能量。