Formation of involatile methylantimony species by Clostridium spp

Trimethylantimony was detected by gas chromatography-mass spectrometry (GC-MS) in the headspace of a soil enrichment culture designed to promote growth of clostridia. Clostridial isolates from the soil enrichment culture were shown to biomethylate inorganic antimony in monseptic culture, using hydride generation-gas chromatographyatomic absorption spectrometry (HG-GC-AAS). GC-MS profiles of headspace gases from soil enrichment cultures shown to generate trimethylantimony, were used to select characterised Clostridium spp for assessment of antimony biomethylation capability. Involatile methylantimony species (up to 21 microg Sb dm(-3)) were detected by HG-GC-AAS in the medium of monoseptic cultures of C. acetobutylicum, C. butyricum and C. cochlearium. The relative quantities of involatile mono-, di- and trimethylantimony species produced over the course of a 28-day cultivation period is consistent with trimethylantimony oxide being a final product of antimony biomethylation by these bacteria, with mono- and dimethylantimony species appearing transiently in the cultures as intermediates of an antimony biomethylation pathway. Clostridia may be the principal agents of antimony biomethylation in methanogenic environments and could give rise to methylated forms of antimony in both the aqueous and gaseous phases.     

Genetic structure of giant clam (Tridacna derasa) populations from reefs in the Indo-Pacific

Large genetic differences were observed among the Great Barrier Reef (GBR), Fiji and Philippine populations of Tridacna derasa (Roding) sampled in 1989 and 1990 (Nei's unbiased genetic distance, D,=0.137 to 0.341). This result contrasted strongly with the low genetic distance (D=0.032) reported previously for the giant clam T. maxima over similar geographical scales. No significant genetic differentiation was observed among most populations from the GBR (mean D=0.007), consistent with the high gene exchange expected in this highly connected reef system. However, significant differentiation resulting from differences in the frequencies of less common alleles between the North-Central GBR and South GBR (Swain region) were observed. Historical isolation of blocks of the Central Indo-West Pacific from the GBR and present-day restrictions to gene exchange between the GBR, Fiji and the Philippines as a result of oceanographic current patterns, were thought to be responsible for the high degree of genetic differentiation of T. derasa populations. The relevance of these findings to clam mariculture and reef restocking are briefly discussed.Contribution No. 197 from the Marine Science Institute, University of the Philippines and Contribution No. 562 from the Australian Institute of Marine Science     

Selective feeding by the mudskipper (<Emphasis Type="Italic">Boleophthalmus pectinirostris</Emphasis>) on the microalgal assemblage of a tropical mudflat

Temporal variation in abundance of the microphytobenthos on an estuarine mudflat in Hong Kong was investigated over a 13-month period. Abundance, in terms of both cell counts and chlorophyll- a (chl- a) concentration, of the diatom-dominated assemblage was higher (peak at 47 mg chl- a m ^-2 in January) in the cooler months than in the warmer months (minimum at 11 mg chl- a m ^-2 in August). Variation in surface density (range: 0.25–1.9 individuals m ^-2) of the herbivorous mudskipper Boleophthalmus pectinirostris was also temporally variable, with a broadly inverse pattern to that of the microphytobenthos. Comparison of the relative abundance of diatom species in the gut and on the mud surface using Strauss' linear selectivity index suggests significant size-selective feeding by B. pectinirostris. Diatoms with maximum lengths >50 µm were selectively ingested whereas smaller species were rejected. This critical diatom length is close to the average distance between branchiospines of the fish's gills, suggesting their involvement in a physical sieving process that is responsible for the size-selective feeding. Significant differences in feeding pattern exist between adult (>80 mm standard length) and juvenile fish. As a result of seasonal variations in density and selective removal of the larger diatoms, B. pectinirostris exerts a significant impact on the abundance and structure of the mudflat microphytobenthos.     

Characterisation of Acid Mine Drainage Using a Combination of Hydrometric, Chemical and Isotopic Analyses, Mary Murphy Mine, Colorado

The legacy of mining continues to affect stream water quality throughout the western United States. Traditional remediation, involving treatment of acid mine drainage from portals, is not feasible for the thousands of abandoned mines in the West as it is difficult and expensive. Thus, the development of new methods to address acid mine drainage is critical. The purpose of this study was two fold; to identify and test new tools to identify sources of metal pollution within a mine and to identify low-cost treatment alternatives through the use of these tools. Research was conducted at the Mary Murphy Mine in Colorado, a multiple-level underground mine which produced gold, silver, copper, lead and zinc from 1870 to 1951. Source waters and flowpaths within the mine were characterised using analysis of hydrogen and oxygen isotopes of water (water isotopes) in combination with solute analysis and hydrometric techniques. Hydrometric measurements showed that while discharge from a central level portal increased by a factor of 10 during snowmelt runoff (from 0.7 to 7.2 Ls^–1), Zn concentrations increased by a factor of 9 (from 3,100 to 28320gL^–1). Water isotopes were used as conservative tracers to represent of baseflow and snowmelt inputs in a hydrologic mixing model analysis. The results showed that less than 7% of peak discharge was from snowmelt. Within the mine, approximately 71% of the high-flow Zn loading was caused by a single internal stream characterised by extremely high Zn concentrations (270600gL^–1) and low pH (3.4). Somewhat surprisingly, hydrologic mixing models using water isotopes showed that new water contributed up to 79% of flow in this high-Zn source during the melt season. Diversion of this high-Zn source within the mine resulted in a decrease in Zn concentrations at the portal by 91% to 2,510gL^–1, which is lower than the base-flow Zn concentration. The results suggest that in some mines remediation efforts can be concentrated on specific areas within the mine itself. Using the characterisation techniques demonstrated in this study, problem areas can be identified and contaminated flows diverted or isolated. The results also suggest that it may be possible to dewater contamination areas, greatly reducing costs of remediation.     

A field-based spectrophotometric flow-injection system for automatic determination of chloride in soil water

The use of chloride as a tracer for soil water investigations is discussed. Limitations with laboratory based soil core and field based sampling are considered with respect to the poor suitability of the data for rigorous assessment of mechanistic models. Investigation of water movement in soil has been restricted by limitations in spatial and temporal sampling. Fine resolution sampling generates large numbers of samples which cause problems with post sampling laboratory analysis. This paper describes a field-based system for the analysis of chloride in soil water. There are three component parts to the system, (i) a custom sampling sub-system comprising of ceramic cup suction samplers and sample traps, (ii) a sample routing sub-system to channel sample to (iii) a sample analysis sub-system utilizing a flow injection method for sample analysis using a custom built photo-diode detector. The three sub-systems were controlled by a suitably equipped personal computer. A calibration procedure is described with a third order polynomial equation derived to convert millivolt response from the detector into chloride concentration. Field and laboratory data from a tracer experiment are presented and discussed, and it is concluded that the system is well suited to field-based applications. Finally it is noted that the photo-detector is suitable for colourimetric analysis of any tracer with suitable chemical determination.     

Effects of geothermal effluents on aquatic ecosystems

The use of geothermal energy for electrical generation and a variety of other purposes requires the handling and disposal of large volumes of geothermal fluids. These fluids contain constituents such as dissolved ammonia, hydrogen sulfide gas, and trace elements. Facilities in the United States are not expected to routinely discharge large volumes of toxic geothermal fluids. However, chronic effects on aquatic ecosystems are possible at concentrations where acute toxicity would be neither expected nor observed. Also, some trace elements may accumulate to hazardous levels in ecosystems, even when released at nontoxic concentrations.     

WATER LOSSES ALONG A REACH OF THE PECOS RIVER IN NEW MEXICO1

ABSTRACT: A reach of the Pecos River, located in eastern New Mexico, was examined to evaluate losses of river flows due to evaporation, seepage, and transpiration. An accurate assessment of the water losses along this reach is critical for determining how water rights are adjudicated for water users in the Pecos basin and interstate compact accounting. Water losses significantly impact flows through critical habitat for species protected under the Endangered Species Act. Daily losses of river flows were analyzed for the study reach that extends from immediately below the Pecos River confluence with Taiban Creek to the United States Geological Survey (USGS) gage near Acme. The analysis was completed with consideration for other processes including flood wave travel times and attenuation along with stream bank storage and returns. The analysis was completed using daily stream flow data from USGS gages located along the study reach. Empirical seasonal functions were developed to relate flow loss to the flow rate in the river. The functions were ultimately developed to provide a method for comparing the effects of different river flows on the available water supply.     

APPLICATION OF A MULTIPURPOSE UNEQUAL PROBABILITY STREAM SURVEY IN THE MID‐ATLANTIC COASTAL PLAIN1

ABSTRACT: A stratified, spatially balanced sample with unequal probability selection was used to design a multipurpose survey of headwater streams in the Mid‐Atlantic Coastal Plain. Objectives for the survey include unbiased estimates of regional stream conditions, and adequate coverage of unusual but significant environmental settings to support empirical modeling of the factors affecting those conditions. The design and field application of the survey are discussed in light of these multiple objectives. A probability (random) sample of 175 first‐order nontidal streams was selected for synoptic sampling of water chemistry and benthic and riparian ecology during late winter and spring 2000. Twenty‐five streams were selected within each of seven hydrogeologic subre‐gions (strata) that were delineated on the basis of physiography and surficial geology. In each subregion, unequal inclusion probabilities were used to provide an approximately even distribution of streams along a gradient of forested to developed (agricultural or urban) land in the contributing watershed. Alternate streams were also selected. Alternates were included in groups of five in each subregion when field reconnaissance demonstrated that primary streams were inaccessible or otherwise unusable. Despite the rejection and replacement of a considerable number of primary streams during reconnaissance (up to 40 percent in one subregion), the desired land use distribution was maintained within each hydrogeologic subregion without sacrificing the probabilistic design.     

WATER QUALITY DURING STORM EVENTS FROM TWO CONSTRUCTED WETLANDS RECEWING MINE DRAINAGE1

ABSTRACT: Flow rates, pH, iron concentration, and manganese concentration were measured during several storm events at two constructed wetlands receiving mine water. During a substantial rain event, flow rates at both the wetland outlets surpassed flow rates at the wetland inlets, reflecting incident rainfall and differences in wetland area at the two sites. A significant positive correlation existed between local rainfall and outflow rates at the larger wetland, but not between rainfall and inflow rates. During storm events, outlet pH, relative to inlet pH, was slightly elevated at the larger wetland, and depressed at the smaller wetland. However, over the course of one year, rainfall was uncorrelated to outlet pH in the larger wetland. A substantial rain event at the smaller wetland resulted in a temporary elevation in outlet iron concentrations, with treatment efficiency reduced to near zero. However, in the larger wetland, outlet iron concentrations were not significantly affected by storm events. Although rainfall and outlet iron concentration were not significant correlates at the larger wetland, flow rate was positively correlated to outlet iron concentration. A normal manganese treatment efficiency of 50 percent at the smaller wetland was reduced to zero during a heavy rain.     

A study on organotin levels in Canadian drinking water distributed through PVC pipes

A study on organotin compounds in Canadian drinking water was carried out in winter-spring 1996 (28 sites) and autumn 1996 (21 sites). Approximately 29% and 40% of distribution waters supplied through PVC pipes installed recently (typically less than 6 months) contained organotin compounds in the winter-spring and autumn surveys respectively. Monomethyl-, dimethyl-, monobutyl- and dibutyltin levels ranged up to 291 ng Sn/L, 49.1 ng Sn/L, 28.5 ng Sn/L and 52.3 ng Sn/L, respectively. An additional study in summer 1996, of locations where the highest organotin levels were detected in the winter-spring survey, indicated that organotin levels had decreased in most distribution water samples. Samples of PVC pipe/tubing contained organotin compounds consistent with the organotin patterns found in the distribution water samples.