A terrain-based paired-site sampling design to assess biodiversity losses from eastern hemlock decline

Biodiversity surveys are often hampered by the inability tocontrol extraneous sources of variability introduced intocomparisons of populations across a heterogenous landscape. If not specifically accounted for a priori, this noisecan weaken comparisons between sites, and can make itdifficult to draw inferences about specific ecologicalprocesses. We developed a terrain-based, paired-sitesampling design to analyze differences in aquaticbiodiversity between streams draining eastern hemlock (Tsuga canadensis) forests, and those draining mixedhardwood forests in Delaware Water Gap National RecreationArea (USA). The goal of this design was to minimize variancedue to terrain influences on stream communities, whilerepresenting the range of hemlock dominated streamenvironments present in the park. We used geographicinformation systems (GIS) and cluster analysis to define andpartition hemlock dominated streams into terrain types basedon topographic variables and stream order. We computedsimilarity of forest stands within terrain types and usedthis information to pair hemlock-dominated streams withhardwood counterparts prior to sampling. We evaluated theeffectiveness of the design through power analysis and foundthat power to detect differences in aquatic invertebratetaxa richness was highest when sites were paired and terraintype was included as a factor in the analysis. Precision ofthe estimated difference in mean richness was nearly doubledusing the terrain-based, paired site design in comparison toother evaluated designs. Use of this method allowed us tosample stream communities representative of park-wide forestconditions while effectively controlling for landscapevariability.     

Spatial patterns in forest composition and standing dead red spruce in montane forests of the Adirondacks and northern Appalachians

The decline of red spruce (Picea rubens Sarg.) in montane forests of the northeastern United States has been previously reported. The objective of this study was to assess spatial patterns, if any, in standing dead red spruce stems in the Adirondacks of New York and northern Appalachians of Vermont, New Hampshire, and Maine. A stratified random sample of 19 mountains along a west to east transect in the Adirondacks and the northern Appalachians showed that the live basal area of all species was highest in the White Mountains (34.6 m² ha^–1) and lowest in the Adirondack Mountains (23.7 m² ha^–1) in the Green Mountains was significantly lower than in any other region. Intact standing dead red spruce in the Adirondack and Green Mountains (30%) was significantly higher than that in the three eastern clusters (14%). The amount of intact standing dead red spruce trees increased with elevation in only the western part of the region. With the exception of the Adirondacks, there was a greater average percent dead red spruce on the west side than on the east side of each mountain. The sum of standing dead for other tree species (average 13%) showed no statistically significant patterns with region, elevation or aspect, and was significantly lower than the amount of total dead red spruce (average 42%). The standing dead red spruce patterns we observed cannot be associated with any specific causal factors at this time.     

Field methods and example applications for the Min-Trap® mineral sampler

Important reactive minerals are commonly created during in situ groundwater remediation activities; for example, iron sulfides formed during enhanced reduction approaches can abiotically degrade many chlorinated solvents. However, cost-effective tools to evaluate these treatment processes in field applications are limited and the collection of samples to evaluate in situ mineral formation is costly due to drilling requirements. The new passive Min-Trap sampler is a simple and cost-effective tool that can directly measure the formation of reactive minerals in situ without the need for additional drilling or soil core collection. The methods presented here describe how Min-Traps deployed in conventional monitoring wells can measure reactive minerals and how these minerals can be identified through commercially available analytical methods. Several examples are presented that show how Min-Traps can be used to characterize the rate and spatial variability of reactive mineral precipitation and these data may support operation and optimization decisions.     

The Adaptation for Conservation Targets (ACT) Framework: A Tool for Incorporating Climate Change into Natural Resource Management

As natural resource management agencies and conservation organizations seek guidance on responding to climate change, myriad potential actions and strategies have been proposed for increasing the long-term viability of some attributes of natural systems. Managers need practical tools for selecting among these actions and strategies to develop a tailored management approach for specific targets at a given location. We developed and present one such tool, the participatory Adaptation for Conservation Targets (ACT) framework, which considers the effects of climate change in the development of management actions for particular species, ecosystems and ecological functions. Our framework is based on the premise that effective adaptation of management to climate change can rely on local knowledge of an ecosystem and does not necessarily require detailed projections of climate change or its effects. We illustrate the ACT framework by applying it to an ecological function in the Greater Yellowstone Ecosystem (Montana, Wyoming, and Idaho, USA)-water flows in the upper Yellowstone River. We suggest that the ACT framework is a practical tool for initiating adaptation planning, and for generating and communicating specific management interventions given an increasingly altered, yet uncertain, climate.     

Physical characterization of the fine particle emissions from commercial aircraft engines during the Aircraft Particle Emissions eXperiment (APEX) 1–3

The fine particulate matter (PM) emissions from nine commercial aircraft engine models were determined by plume sampling during the three field campaigns of the Aircraft Particle Emissions Experiment (APEX). Ground-based measurements were made primarily at 30 m behind the engine for PM mass and number concentration, particle size distribution, and total volatile matter using both time-integrated and continuous sampling techniques. The experimental results showed a PM mass emission index (EI) ranging from 10 to 550 mg kg^?1 fuel depending on engine type and test parameters as well as a characteristic U-shaped curve of the mass EI with increasing fuel flow for the turbofan engines tested. Also, the Teflon filter sampling indicated that ～40–80% of the total PM mass on a test-average basis was comprised of volatile matter (sulfur and organics) for most engines sampled. The number EIs, on the other hand, varied from ～10¹⁵ to 10¹⁷ particles kg^?1 fuel with the turbofan engines exhibiting a logarithmic decay with increasing fuel flow. Finally, the particle size distributions of the emissions exhibited a single primary mode that were lognormally distributed with a minor accumulation mode also observed at higher powers for all engines tested. The geometric (number) mean particle diameter ranged from 9.4 to 37 nm and the geometric standard deviation ranged from 1.3 to 2.3 depending on engine type, fuel flow, and test conditions.     

The effects of 3,3′,4,4′-tetrabromobiphenyl on rats fed diets containing a constant level of copper and varying levels of molybdenum

Copper (Cu) metabolism is altered in rats fed diets high in molybdenum (Mo) and low in Cu. This 10-week study was carried out to examine the effects of supplemental Mo (7.5–240 μg/g diet) on male Sprague–Dawley rats fed diets adequate in Cu (5 μg/g diet) and to determine the susceptibility of Mo-treated animals to the environmental pollutant 3,3′,4,4′-tetrabromobiphenyl (TBB). After 7 weeks of dietary treatment, half of the rats in each group received a single IP injection of TBB (150 μM/kg bw), while the other half received the corn oil vehicle. Rats sacrificed at 10 weeks showed no effects of Mo on growth, feed efficiency, or selected organ or tissue weights. Dose-dependent effects on plasma Mo (0–5.1 μg/mL), plasma Cu (0.95–0.20 μg/mL), and bone Cu (3.4–10 μg/g) in control through the high dose were found. Cu sequestration in the bone of Mo-treated rats is a new finding. TBB treatment resulted in dramatic weight loss and loss of absolute organ mass. Relative organ weights were increased, except for the thymus. TBB altered the concentrations of certain amino acids. Compared to control rats, this polybrominated biphenyl congener significantly decreased plasma Cu and ceruloplasmin at higher concentrations of dietary Mo and promoted the process of plasma Cu decrease by Mo, suggesting a combined effect.     

Development and Integration of Quantitative Real-Time PCR Methods for Detection of Mitochondrial DNA and Methanobrevibacter smithii nifH Gene as Novel Microbial Source Tracking Tools

A novel microbial source tracking (MST) method based on the detection of human and non-human markers was developed and applied to track the origin of fecal pollution in water systems. Mitochondrial DNA sequences were used to develop new quantitative real-time polymerase chain reaction (qPCR) assays for dog, poultry, and gull. The targets were included as part of a toolbox including human, cow, pig, and sheep assays. A primer and probe set for the detection of the human-specific nifH gene of Methanobrevibacter smithii was also designed as an indicator of human fecal contamination. The assays were tested for specificity and applied to fecal-spiked surface waters and environmental samples collected from two river catchments impacted by sources of human and non-human fecal contamination. The MST methods described were applicable to both spiked waters and environmental samples, and using the two approaches the origin of fecal pollution could be successfully determined in mixed source fecally polluted waters.     

Conservation Applications of Astronaut Photographs of Earth: Tidal-Flat Loss ( Japan), Elephant Effects on Vegetation (Botswana), and Seagrass and Mangrove Monitoring (Australia)

Abstract: National Aeronautics and Space Administration ( NASA) photographs taken by astronauts from low Earth orbit can provide information relevant to conservation biology. This data source is now more accessible because of improvements in digitizing technology, Internet file transfer, and availability of image processing software. We present three examples of conservation-related projects that benefited from the use of astronaut photographs. First, NASA scientists requested that astronauts photograph the area of the controversial Isahaya Bay reclamation project in Japan. Japanese researchers used photographs from before and after the reclamation as a tool for communication with the public about the effects of tidal-flat loss. The newly acquired images and the availability of high-resolution digital images from NASA archives provided timely public information on the observed changes. Second, we digitally classified and analyzed a Space Shuttle photograph of Chobe National Park in Botswana to identify the locations of woodlands affected by elephants. Field validation later confirmed that areas identified on the image showed evidence of elephant damage. Third, we used a summary map from intensive field surveys of seagrasses in Shoalwater Bay, Australia, as reference data for a supervised classification of a digitized photograph taken from orbit. The classification distinguished seagrasses, sediments, and mangroves with accuracy approximating that in studies using other satellite remote-sensing data. Astronaut photographs are in the public domain, and the database of nearly 400,000 photographs from the late 1960s to the present is available at a single searchable location on the Internet (  http://eol.jsc.nasa.gov/sseop ). These photographs can be used by conservation biologists as a source of general information about the landscape and for quantitative mapping.     

Measurement of organic contaminants and biological effects in Scottish waters between 1999 and 2005

Concentrations of organic contaminants were determined in sediment and fish collected annually at six sites around Scotland between 1999 and 2005, as part of the UK National Marine Monitoring Programme. Polycyclic aromatic hydrocarbons (PAHs) and chlorobiphenyls (CBs) were measured in sediment, while CBs and ethoxyresorufin-O-deethylase (EROD) activities were measured in the fish. Highest PAH concentrations in sediment were found at sites where higher coastal influences would be expected (e.g., Clyde and Solway) and four of the six sites gave PAH concentrations above OSPAR background assessment concentrations (BACs). A significant downward trend in the median total PAH concentrations, normalised to total organic carbon (TOC), was found at one of the six sites (Minches). The PAH profiles at all sites were consistent over the 6 year period, indicating that the sites are relatively stable and PAH sources are not changing. There was an indication of a greater petrogenic input on the west coast, with sediment from the Clyde, Solway and Minches having a greater proportion of 2- and 3-ring PAHs and a lower proportion of 5-ring PAHs. CB concentrations at all sites were low compared with UK estuarine sites and similar to sediment from more remote areas: however, BACs were exceeded. No significant trends were detected in either the sediment CB concentrations or patterns at any of the six sites. CB concentrations were significantly higher in fish liver collected from the Clyde. Concentrations at the other five sites were low, with the majority of samples having concentrations for the ICES7 CBs of <500 microg kg(-1) lipid weight. However, individual CB concentrations were still above the BACs. Hepatic EROD activities were measured in male plaice from 2002-2005 and were generally low (<10 pmol min(-1) mg protein(-1)). No temporal trends were detected in either CB concentrations or the EROD activity.