Oxidation of natural and synthetic hormones by the horseradish peroxidase enzyme in wastewater

Steroid estrogens, including both natural estrogens (e.g., estrone - E1; 17beta-estradiol - E2; and estriol - E3) and synthetic estrogens (e.g., 17alpha-ethinylestradiol - EE2), are known as endocrine-disrupting compounds. The objective of this research was to evaluate the feasibility of the enzymatic oxidation of estrogens and to optimize this process in municipal wastewater contaminated with steroid estrogens using horseradish peroxidase (HRP) and hydrogen peroxide. An initial HRP activity of 0.02 U ml(-1) was sufficient to completely remove EE2 from the synthetic solution, although greater HRP doses (up to 0.06 U ml(-1)) were required to remove E1, E2 and E3. The optimal molar peroxide-to-substrate ratio was determined to be approximately 0.45. Based on the Michaelis-Menten kinetics, the HRP had an increasing reactivity with E1, E3, E2, and EE2, in increasing order. In real activated sludge process effluent, an HRP dose of 8-10 U ml(-1) was required to completely remove all of the studied estrogens, while only 0.032 U ml(-1) of HRP was necessary to treat synthetic water containing the same estrogen concentrations.     

The perpetual state of emergency that sacrifices protected areas in a changing climate

A modern challenge for conservation biology is to assess the consequences of policies that adhere to assumptions of stationarity (e.g., historic norms) in an era of global environmental change. Such policies may result in unexpected and surprising levels of mitigation given future climate‐change trajectories, especially as agriculture looks to protected areas to buffer against production losses during periods of environmental extremes. We assessed the potential impact of climate‐change scenarios on the rates at which grasslands enrolled in the Conservation Reserve Program (CRP) are authorized for emergency harvesting (i.e., biomass removal) for agricultural use, which can occur when precipitation for the previous 4 months is below 40% of the normal or historical mean precipitation for that 4‐month period. We developed and analyzed scenarios under the condition that policy will continue to operate under assumptions of stationarity, thereby authorizing emergency biomass harvesting solely as a function of precipitation departure from historic norms. Model projections showed the historical likelihood of authorizing emergency biomass harvesting in any given year in the northern Great Plains was 33.28% based on long‐term weather records. Emergency biomass harvesting became the norm (>50% of years) in the scenario that reflected continued increases in emissions and a decrease in growing‐season precipitation, and areas in the Great Plains with higher historical mean annual rainfall were disproportionately affected and were subject to a greater increase in emergency biomass removal. Emergency biomass harvesting decreased only in the scenario with rapid reductions in emissions. Our scenario‐impact analysis indicated that biomass from lands enrolled in the CRP would be used primarily as a buffer for agriculture in an era of climatic change unless policy guidelines are adapted or climate‐change projections significantly depart from the current consensus.     

A framework for evaluating the impact of the IUCN Red List of threatened species

The International Union for Conservation of Nature (IUCN) Red List of Threatened Species, a species extinction risk assessment tool, has been guiding conservation efforts for over 5 decades. It is widely assumed to have been instrumental in preventing species from moving closer to extinction and driving recoveries. However, the impact of the IUCN Red List in guiding conservation has not been evaluated. We conducted, transcribed, and coded interviews with experts who use the IUCN Red List across a range of sectors to understand how the list is used in conservation. We developed a theory of change to illustrate how and why change is expected to occur along causal pathways contributing to the long-term goal of the IUCN Red List and an evaluation framework with indicators for measuring the impact of the IUCN Red List in generating scientific knowledge, raising awareness among stakeholders, designating priority conservation sites, allocating funding and resources, influencing development of legislation and policy, and guiding targeted conservation action (key themes). Red-list assessments were the primary input leading to outputs (scientific knowledge, raised awareness), outcomes (better informed priority setting, access to funding and resource availability, improved legislation and policy), and impact (implemented conservation action leading to positive change) that have resulted in achievement of IUCN Red List goals. To explore feasibility of attributing the difference made by the IUCN Red List across themes, we studied increased scientific knowledge, raised awareness, access to funding and resource allocation, and increased conservation activity. The feasibility exploration showed increased scientific knowledge over time identified through positive trends in publications referring to the IUCN Red List in the literature; raised awareness of the list following high IUCN activity identified by peaks in online search activity; an increased proportion of conservation funding bodies requesting IUCN Red List status in the application process; and, based on interviews with Amphibian Specialist Group members, red-list assessments were essential in connecting relevant stakeholders and ensuring conservation action. Although we identified the IUCN Red List as a vital tool in global conservation efforts, it was challenging to measure specific impacts because of its ubiquitous nature. We are the first to identify the influence of the IUCN Red List on conservation.     

Environmental News in Argentina,Chile, Mexico and the Philippines

From an environmental perspective, the media have raised the level of environmental awareness and, rightly or wrongly, focused the attention of citizens on specific environmental issues. Public reaction to environmental news can filter back into the media and highlight specific policy issues. The importance of the media in this respect has gained strength, particularly in developing countries, where environmental problems have become legitimate concerns, and where access to 'official' information has been historically difficult. In general, newspapers have been found to be one of the most predominant sources of environmental information available to the public, particularly in developing countries. In this paper, we summarise, briefly analyse and compare the newspaper coverage of environmental issues in Argentina, Chile, Mexico and the Philippines. Among numerous other results, it appears that the coverage of environmental issues fell considerably over the period 1990-94 despite an increase in world-wide environmental awareness. Most news reports actions by the environmental agencies (warnings and rewards, etc.). In the Philippines, however, most environmental news reports investments in pollution abatement activities by the private sector. While citizens' complaints represent a small percentage of the coverage of environmental issues, closer examination reveals that such complaints, in the vast majority of cases, trigger governmental actions (warnings) and ultimately an improvement of firms' environmental performance (investment).     

Physiological response of the cold-seep mussel Bathymodiolus childressi to acutely elevated temperature

It is predicted that deep-sea animals adapted to thermally stable conditions should be highly sensitive to temperature change and should not have inducible heat-shock responses. This premise was tested with the cold-seep mussel Bathymodiolus childressi Gustafson, 1998 from 750 m depth in the Gulf of Mexico at a site known as Brine Pool NR-1 (27°43.4157N, 91°16.756W). Mussels were collected during February 2003. Site temperature, measured in different months between 1995 and 2005, ranged between 6.5 and 7.2°C. Although Brine Pool NR-1 is stenothermal, hydrogen sulfide, oxygen, and salinity vary over temporal and spatial scales. In laboratory experiments, B. childressi survived increases up to approximately 20°C above ambient temperature for 6 h before suffering greater than 50% mortality. Although a high thermal tolerance was observed, B. childressi did not express an inducible 70 kDa heat-shock protein. However, high constitutive levels of hsp70 were present in B. childressi suggesting a necessity to remediate protein damage from stressors other than elevated temperature; these constitutive proteins probably confer an indirect thermal tolerance.     

Use of Riparian Buffer Strips as Movement Corridors by Forest Birds

We conducted a 3-year field experiment to measure the frequency of bird movements through riparian buffer strips before and after harvesting of adjacent forest. Our study was conducted in the boreal mixed wood forest of Alberta and was designed to determine empirically whether songbirds use riparian buffer strips of forest connecting forest reserves as corridors and if they move along these buffer strips more frequently than they cross adjacent clearcuts. We used mist nets to obtain an index of the frequency of bird movement in the forest, and we observed bird movements across adjacent clearcuts for comparison. We predicted that the frequency of movement would be greater (1) in buffer strips after harvesting of adjacent forest than before harvesting, (2) in buffer strips than across clearcuts and, (3) in buffer strips than at control sites (lakeshore forest with no adjacent clearcuts). After adjusting for year-to-year variation in abundance, we found that capture rates increased significantly from pre- to post-harvest, but only for juveniles. Capture rates of adults decreased immediately after harvesting, probably because of the removal of an adjacent source of birds that previously moved through the lakeside forest. Movement rates of forest species in clearcuts were significantly lower than capture rates in the forest. The number of adults captured was positively correlated with the number of territories in the buffer strips, indicating that most birds captured were probably residents. The number of local territories was a poor predictor of juvenile captures, supporting the notion that juveniles were likely dispersing individuals. Our results indicate that buffer strips enhanced movements of juveniles (i.e., acted as corridors) and maintained movement rates of adults. Furthermore, there appeared to be a threshold distance between reserves below which birds may be less reluctant to fly across openings, making corridor use less important.     

Microscale genetic differentiation in a sessile invertebrate with cloned larvae: investigating the role of polyembryony

Microscale genetic differentiation of sessile organisms can arise from restricted dispersal of sexual propagules, leading to isolation by distance, or from localised cloning. Cyclostome bryozoans offer a possible combination of both: the localised transfer of spermatozoa between mates with limited dispersal of the resulting larvae, in association with the splitting of each sexually produced embryo into many clonal copies (polyembryony). We spatially sampled 157 colonies of Crisia denticulata from subtidal rock overhangs from one shore in Devon, England at a geographic scale of ca. 0.05 to 130 m plus a further 21 colonies from Pembrokeshire, Wales as an outgroup. Analysis of molecular variance (AMOVA) revealed that the majority (67%) of genetic variation was distributed among individuals within single rock overhangs, with only 16% of variation among different overhangs within each shore and 17% of variation between the ingroup and outgroup shores. Despite local genetic variation, pairwise genetic similarity analysed by spatial autocorrelation was greatest at the smallest inter-individual distance we tested (5 cm) and remained significant and positive across generally within-overhang comparisons (<4 m). Spatial autocorrelation and AMOVA analyses both indicated that patches of C. denticulata located on different rock overhangs tended to be genetically distinct, with the switch from positive to negative autocorrelation, which is often considered to be the distance within which individuals reproduce with their close relatives or the radius of a patch, occurring at the 4–8 m distance class. Rerunning analyses with twenty data sets that only included one individual of each multilocus genotype (n = 97) or the single data set that contained just the unique genotypes (n = 67) revealed that the presence of repeat genotypes had an impact on genetic structuring (PhiPT values were reduced when shared genotypes were removed from the dataset) but that it was not great and only statistically evident at distances between individuals of 1–2 m. Comparisons to a further 20 randomisations of the data set that were performed irrespective of genotype (n = 97) suggested that this conclusion is not an artefact of reduced sample size. A resampling procedure using kinship coefficients, implemented by the software package GENCLONE gave broadly similar results but the greater statistical power allowed small but significant impacts of repeat genotypes on genetic structure to be also detected at 0.125–0.5 and 4–16 m. Although we predict that a proportion of the repeat multilocus genotypes are shared by chance, such generally within-overhang distances may represent a common distance of cloned larval dispersal. These results suggests that closely situated potential mates include a significant proportion of the available genetic diversity within a population, making it unlikely that, as previously hypothesised, the potential disadvantage of producing clonal broods through polyembryony is offset by genetic uniformity within the mating neighbourhood. We also report an error in the published primer note of Craig et al. (Mol Ecol Notes 1:281–282, 2001): loci Cd5 and Cd6 appear to be the same microsatellite. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users.     

Remediation of MTBE from drinking water: air stripping followed by off-gas adsorption

The widespread use of methyl tertiary butyl ether (MTBE) as an oxygenate in gasoline has resulted in the contamination of a large number of ground and surface water sources. Even though air stripping has been proven to be an effective treatment technology for MTBE removal, off-gas treatment often is required in conjunction with it. This study evaluated the combined treatment technologies of air stripping followed by off-gas adsorption on a pilot scale for the treatment of MTBE-contaminated water. The effect of air/water ratios on the treatment efficiency was studied, and the mass transfer coefficient was determined. Air/water ratios of 105:1, 151:1, 177:1, 190:1, 202:1, and 206:1 were used, and a treatment efficiency of >99% was achieved for all the runs conducted. The depth of packing required to achieve maximum treatment efficiency decreased with increasing air/water ratio. Relative humidity (RH) impacts on the MTBE adsorption capacity of a granular activated carbon (GAC) and carbonaceous resin were determined from pilot plant studies. Breakthrough profiles obtained from the pilot plant studies conducted at 20, 30, and 50% RH indicated that GAC has a higher adsorptive capacity than resin. The adsorptive capacity of GAC decreased with increasing RH, whereas RH did not impact the resin adsorptive capacity.