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281.
282.
The polymerase chain reaction (PCR) was used on material from a blighted ovum to confirm indirectly the carrier status of a woman with a family history of Becker muscular dystrophy. Conventional testing including creatine kinase levels, muscle biopsy, and EMG had been inconclusive, and on the basis of one elevated creatine kinase level, the woman had been designated a possible carrier. Ultrasound examination at 10 weeks of pregnancy indicated a blighted ovum, from which DNA was subsequently extracted and subjected to PCR testing for determination of sex and genotypic status with respect to the known familial deletion of the dystrophin gene. The blighted ovum was found to have a Y chromosome and also to be deleted for at least exon 6 of the dystrophin gene, indirectly indicating that the mother most likely carried the family mutation for Becker muscular dystrophy. 相似文献
283.
284.
Baisen Zhang Ian Valentine Peter D. Kemp 《Agriculture, ecosystems & environment》2005,110(3-4):279-288
Decision tree, one of the data mining approaches, was used to model the relative abundance of five functional groups of plant species, namely high fertility response grasses (HFRG), low fertility tolerance grasses (LFTG), legume, moss and flatweeds in a New Zealand hill-pasture ecosystem using aboveground biomass. The model outputs were integrated with a geographic information system (GIS) to map and validate the predictions on a pasture. The decision tree models clearly revealed the interactions between the functional groups and environmental and management factors, and also indicated the relative importance of these factors in influencing the functional group abundance. Soil Olsen P was the most significant factor influencing the abundance of LFTG and moss, while soil bulk density, slope and annual P fertiliser input were the most significant factors influencing the abundance of legume, HFRG and flatweeds, respectively. Generally, slope and soil Olsen P were the two key factors underlying the patterns of abundance for these five functional groups. For the five functional groups studied, there was an overall predictive accuracy of 75%. Modelling functional group abundance simplified the investigation of the complex interrelationship between species and environment in a pasture ecosystem. The integration of the decision tree with GIS in this study provides a platform to investigate community structure and functional composition for a pasture over space, and thus can be applied as a tool in pasture management. 相似文献
285.
IntroductionFertilizationisessentialforagriculturalproduction ,butunreasonablyapplyingfertilizerscouldresultinseriesofproblemssuchascropqualitydecline,fertilizeruseefficiencydecrease,residualfertilizerspollution groundwaterandriversandnitrogencontented … 相似文献
286.
厦门西港海水质量对鱼卵胚胎发育畸形率的影响 总被引:3,自引:1,他引:3
以养殖场培育的花尾胡椒鲷受精卵为实验材料测试了厦门西域5个站位的表层水水样对花尾胡椒鲷受精卵的胚胎发育毒性,并运用GC-MS手段分析了水样中的美国EPA优控的16PAHs组分的含量。结果表明:3号站位(厦门市工业和生活污水主要排放口的外侧)和4号站位(厦门西港的港区)的水样对鱼卵的胚胎发育的毒性最强。8号站位(位于主航道并濒临嵩屿电厂)的水样对鱼卵胚胎发良的毒性次之,1号站位(湾口)和5号站位(内港)的水样对鱼卵的胚胎发育的毒性较小。水样中的PAHs含量分析结果表明,仅用水样中的PAHs含量不能全面地反映水样对鱼卵胚胎发育的毒性。最后,对本实验方法应用于海洋环境生物监测的有效性进行了探讨。 相似文献
287.
Farideh Z. Bischoff PhD Dorothy E. Lewis Joe Leigh Simpson Dianne D. Nguyen Jeffrey Scott Wendy Schober Sarah Murrell Sherman Elias 《黑龙江环境通报》1995,15(12):1182-1184
Recovering and analysing fetal erythrocytes from maternal blood is being pursued for non-invasive prenatal genetic diagnosis. We report the observation of 46, XY/47, XXY mosaicism in fetal cells from a woman whose first-trimester chorionic villus sampling (CVS) initially showed only 46, XY. Only after exhaustive (500 cells) analysis were four XXY cells found in cultured villi. 相似文献
288.
Mr. M. T. Rebello G. Hackett J. Smith F. E. Loeffler S. Robson N. Maclachlan R. W. Beard C. H. Rodeck R. Williamson D. V. Coleman C. Williams 《黑龙江环境通报》1991,11(1):41-46
Ten-ml samples of amniotic fluid were taken from pregnancies being terminated at 8–14 weeks' gestation. DNA was extracted from the amniotic cells by sequential centrifugation and analysed using the polymerase chain reaction (PCR). Fifteen samples were analysed for evidence of maternal contamination using Mfd5 oligo-nucleotide primers for repeat polymorphisms. Ten amniotic fluid samples were tested for the Delta-F508 deletion characteristic of cystic fibrosis to demonstrate a diagnostic application for the technique. In each case, DNA extracted from fetal tissue from the same pregnancy was included in the controls. In 14 of the 15 cases tested with the Mfd5 primers, both the amniotic fluid DNA and the fetal DNA showed no evidence of contaminating DNA. In one case, neither the amniotic fluid cells nor the fetal cells yielded results. In nine of the ten cases tested with the Delta-F508 primers, the amniotic fluid cell DNA provided accurate information about the genetic status of the fetus; in the tenth, the fetal DNA failed to amplify. The results indicate that adequate DNA can be extracted from amniotic fluid from 8 weeks' gestation onward and these samples are suitable for prenatal diagnosis using PCR. 相似文献
289.
A pleural effusion associated with congenital pulmonary lymphangiectasia was detected in a fetus in utero but was absent at the time of delivery. The pleural effusion was unilateral although the disease involved both lungs. In this case there was an association between polyhydramnios and congenital pulmonary lymphangiectasia. 相似文献
290.