城市空气中多环芳烃定量测定方法

本文发展了一种对城市空气中多环芳烃(PAH)定量测定的方法。该方法可以用极少量溶剂,相当快地完成测定工作。     

用广义λ—分布拟合空气污染数据

广义λ-分布是一个四参数可变通的概率模型,包括多种单变量分布,这一模型可用于表示空气污染浓度的频率分布。本文采用土耳其安卡拉每日的烟和SO_2监测数据,来证明广义λ-分布具有良好的拟合性质。这里还讨论了与分位数估计和拟合优度有关的一些结果,并与经典对数正态分布进行了比较。     

Characterization of microbial communities from coastal waters using microarrays

Molecular methods, including DNA probes, were used to identify and enumerate pathogenic Vibrio species in the Chesapeake Bay; our data indicated that Vibrio vulnificus exhibits seasonal fluctuations in number. Our work included a characterization of total microbial communities from the Bay; development of microarrays that identify and quantify the diversity of those communities; and observation of temporal changes in those communities. To identify members of the microbial community, we amplified the 16S rDNA gene from community DNA isolated from a biofilm sample collected from the Chesapeake Bay in February, 2000. The resultant 75 sequences were 95% or more similar to 7 species including two recently described Shewanella species, baltica and frigidimarina, that have not been previously isolated from the Chesapeake. When the genera of bacteria from biofilm after culturing are compared to those detected by subcloning amplified 16S fragments from community DNA, the cultured sample exhibited a strong bias. In oysters collected in February, the most common bacteria were previously unknown. Based on our 16S findings, we are developing microarrays to detect these and other microbial species in these estuarine communities. The microarrays will detect each species using four distinct loci, with the multiple loci serving as an internal control. The accuracy of the microarray will be measured using sentinel species such as Aeromonas species, Escherichia coli, and Vibrio vulnificus. Using microarrays, it should be possible to determine the annual fluctuations of bacterial species (culturable and non-culturable, pathogenic and non-pathogenic). The data may be applied to understanding patterns of environmental change; assessing the health of the Bay; and evaluating the risk of human illness associated with exposure to and ingestion of water and shellfish.     

Biodegradation of undecylbenzenesulphonate by mixed methane-oxidizing culture

Biodegradation of undecylbenzenesulphonate (C(11)LAS) was performed in shake flasks at 21 degrees C by using a mixed methanotrophic-heterotrophic culture containing type II methanotrophs. Concentrations of C(11)LAS and aromatic intermediates were determined by reversed-phase high-performance liquid chromatography (RP-HPLC). Methane and carbon dioxide concentrations were measured in headspace samples by using gas chromatography. RP-HPLC analyses of aqueous samples show that the culture MM1 expresses the capability of C(11)LAS transformation in the presence or absence of methane. Simultaneous methane oxidation and C(11)LAS degradation, and the inhibition of both transformation processes by acetylene were observed. This suggests the possibility that C(11)LAS transformation is catalyzed by methane monooxygenase (MMO). Comparable affinity of culture MM1 for both methane and C(11)LAS ( [Formula: see text], respectively), and more than four times higher maximum transformation rate for methane than for C(11)LAS ( [Formula: see text] (dry weight) cells day(-1), respectively), were determined. This further supports the involvement of MMO enzyme system in transformation and suggests a pronounced competitive inhibition of C(11)LAS degradation by methane.     

原子吸收法测定矿化水中的银

<正> 由于检出限低,准确度高和分析速度快,在测定银的分析方法中原子吸收光谱法得到广泛应用。例如,在低矿化天然水中银的相对检出限为0.03μg/L。用无火焰原子吸收法直接测定高矿化水中的银有困难,因为基体效应大。消除此效应的最有效的方法是利用炉温稳定的平台技术。实现     

菲律宾制糖工业废水的厌氧处理

1.前言菲律宾制糖工业在国民经济中占有重要地位,甘蔗种植面积达50万公顷,在1983～84年度的制糖季节里,全国41个糖厂的蔗糖产量为230万吨。在粗糖生产中的主要副产物有:(1)糖蜜,通常用来生产酒精;(2)蔗渣,通常用来作燃料或者造纸;(3)滤泥,通常用来加工成有机肥料。在粗糖、精制糖与酒精的生产过程中要产生大量废水,通常都将这类废水排入工厂附近的河流或港湾中,常常给这些水域造成难以承受的环境污染。菲律宾国家污染控制委员会最近发布命令指出,不久的将来将颁布控制地表水污染的法规。为此,菲律宾工业产生的废水必须进行处理才能向外排放,有些糖厂已经设置了废水处理设施。传统的废水处理装置通常包括曝气池或贮水塘,然而,因为能源耗用量大,曝气系统的费用很高。     

Distribution of actinomycetes in oil contaminated ultisols of the Niger Delta (Nigeria)

The distribution of actinomycetes in oil contaminated sandy loam ultisols of the Niger Delta region of Nigeria was studied to aid in understanding the effect of hydrocarbons on indigenous microbial population in tropical soils. The average total counts of actinomycetes in all the oil samples analysed was 103 cfu/g. Higher counts of actinomycetes were observed during the dry season than during the wet season. The counts of hydrocarbonoclastic actinomycetes correlated positively with the total count of actinomycetes.The actinomycetes were generally restricted to the top soil(0-10 cm soil depth) although a seemingly deeper(down to 40 cm soil depth) distribution was noticed in the dry season. The isolates included oil degrading species of Actinoplanes, Norcadia,Streptomyces and Streptosporangium. Their high oil utilization ability indicates their positive potential and role in the bioremediation of oil-spilled soils.     

远东的水质污染(下)

在远东国家的水生生态系中,保守型污染物方面的可用资料少于有机物方面的资料。由于痕量金属和有机氯或碳水化合物的测定技术要求很高,远东的许多设施还不能提供所需的仪器。除此而外,该地区所面临的明显问题是加强仪器的维护。尽管如此,