Bioaccumulation and biotransformation of arsenic compounds in Hediste diversicolor (Muller 1776) after exposure to spiked sediments

This study focused on the exposure of the common ragworm Hediste diversicolor (Müller 1776) to sediments enriched with different arsenic compounds, namely arsenate, dimethyl-arsinate, and arsenobetaine. Speciation analysis was carried out on both the spiked sediments and the exposed polychaetes in order to investigate H. diversicolor capability of arsenic bioaccumulation and biotransformation. Two levels of contamination (acute and moderate dose) were chosen for enriched sediments to investigate possible differences in the arsenic bioaccumulation patterns. The highest value of arsenic in tissues was reached after 15 days of exposure to dimethyl-arsinate (acute dose) spiked sediment (1,172?±?176 μg/g). A significant increase was also obtained in worms exposed both to arsenate and arsenobetaine. Speciation analysis showed that trimethyl-arsine oxide was the predominant chemical form in tissues of H. diversicolor exposed to all the spiked sediments, confirming the importance of this intermediate in biological transformation of arsenic.     

A multidisciplinary weight of evidence approach for classifying polluted sediments: Integrating sediment chemistry, bioavailability, biomarkers responses and bioassays

Evaluation of chemical bioavailability and onset of biological alterations is fundamental to assess the hazard of environmental pollutants, particularly when associated to sediments which need to be removed. In the present work, five sediment samples were collected from the Venice Lagoon and data from sediment chemistry were integrated with those of bioaccumulation of chemicals in European eel (Anguilla anguilla) exposed under laboratory conditions, responses of a wide battery of biomarkers, and standardized ecotoxicological bioassays. The overall results were elaborated within a recently developed, software-assisted weight of evidence (WOE) model which provides synthetic indices for each of considered line of evidence (LOE), before a general evaluation of sediment hazard. Levels of chemicals in sediments were not particularly elevated when compared to sediment quality guidelines of Venice Protocol. On the other hand, bioavailability was evident in some samples for Cd, Cu, Zn and, especially, polycyclic aromatic hydrocarbons. The ecotoxicological approach provided further evidence on the biological and potentially harmful effects due to released contaminants, and oxidative-mediated responses appeared of primary importance in modulating sublethal responses and the onset of cellular alterations. Biomarkers variations were sensitive, and more evident variations included significant changes of cytochrome P450 biotransformation pathway, antioxidant responses, onset of oxidative damages, lysosomal membrane stability and genotoxic effects. The results obtained from the battery of bioassays indicated that responses measured at organism level were in general accordance but less marked compared to the onset of sublethal changes measured through biomarkers. Overall this study revealed differences when comparing evaluations obtained from different LOEs, confirming the importance of considering synergistic effects between chemicals in complex mixtures. Compared to a qualitative pass-fail approach toward normative values, the proposed WOE model allowed a quantitative characterization of sediment hazard and a better discrimination of on the basis of various types of chemical and biological data.     

Ecotoxicological effects of sediments from Mar Piccolo,South Italy: toxicity testing with organisms from different trophic levels

The Mar Piccolo of Taranto (Ionian Sea, Southern Italy) is a semi-enclosed and strongly polluted basin. For decades, it has been subjected to different anthropogenic impacts. These stressors caused severe sediments contamination with high concentration of different pollutants (PAHs, PCB, heavy metals). In order to assess the current status of sediments contamination, an ecotoxicological investigation combined with chemical analysis (heavy metals, PAH, and PCB) has been performed. In order to derive ecologically relevant conclusions, a multiorganisms and multiend-points approach has been applied, exposing organisms from different trophic levels to elutriate and whole sediment. The battery of bioassays consists of a microalgal growth inhibition test (Dunaliella tertiolecta), acute and sublethal assays (end-points: mortality, immobilization and swimming speed alteration) on crustaceans larvae and juveniles, and rotifers (Amphibalanus amphitrite, Artemia salina, Corophium insidiosum and Brachionus plicatilis), and embryotoxicity test on echinoderms (Paracentrotus lividus). Considering the high levels of sediment contamination highlighted from chemical analysis, an unexpected very low toxic effect was observed, even considering the sublethal end-point (larval swimming speed alteration). The results of this study suggest a very complex contaminants dynamic in the Mar Piccolo sediments that, despite a strong level of contamination, seems to not affect in a proportional manner the biological compartment.     

Assessing sediment hazard through a weight of evidence approach with bioindicator organisms: a practical model to elaborate data from sediment chemistry, bioavailability, biomarkers and ecotoxicological bioassays

Quality assessments are crucial to all activities related to removal and management of sediments. Following a multidisciplinary, weight of evidence approach, a new model is presented here for comprehensive assessment of hazards associated to polluted sediments. The lines of evidence considered were sediment chemistry, assessment of bioavailability, sub-lethal effects on biomarkers, and ecotoxicological bioassays. A conceptual and software-assisted model was developed with logical flow-charts elaborating results from each line of evidence on the basis of several chemical and biological parameters, normative guidelines or scientific evidence; the data are thus summarized into four specific synthetic indices, before their integration into an overall sediment hazard evaluation. This model was validated using European eels (Anguilla anguilla) as the bioindicator species, exposed under laboratory conditions to sediments from an industrial site, and caged under field conditions in two harbour areas. The concentrations of aliphatic hydrocarbons, polycyclic aromatic hydrocarbons and trace metals were much higher in the industrial compared to harbour sediments, and accordingly the bioaccumulation in liver and gills of exposed eels showed marked differences between conditions seen. Among biomarkers, significant variations were observed for cytochrome P450-related responses, oxidative stress biomarkers, lysosomal stability and genotoxic effects; the overall elaboration of these data, as those of standard ecotoxicological bioassays with bacteria, algae and copepods, confirmed a higher level of biological hazard for industrial sediments. Based on comparisons with expert judgment, the model presented efficiently discriminates between the various conditions, both as individual modules and as an integrated final evaluation, and it appears to be a powerful tool to support more complex processes of environmental risk assessment.     

Agricultural and green infrastructures: the role of non-urbanised areas for eco-sustainable planning in a metropolitan region

Non-Urbanised Areas (NUAs) are part of agricultural and green infrastructures that provide ecosystem services. Their role is fundamental for the minimization of urban pollution and adaptation to climate change. Like all natural ecosystems, NUAs are endangered by urban sprawl. The regulation of sprawl is a key issue for land-use planning. We propose a land use suitability strategy model to orient Land Uses of NUAs, based on integration of Land Cover Analysis (LCA) and Fragmentation Analysis (FA). With LCA the percentage of evapotranspiring surface is defined for each land use. Dimensions and densities of NUAs patches are assessed in FA. The model has been developed with Geographical Information Systems, using an extensive set of geodatabases, including orthophotos, vectorial cartographies and field surveys. The case of the municipality of Mascalucia in Catania metropolitan area (Italy), characterized by a considerable urban sprawl, is presented.     

Sowing simulation tests of a pneumatic drill equipped with systems aimed at reducing the emission of abrasion dust from maize dressed seed

The utilization of dressed seed for spring sowing is a widespread practice to control some pests with reduced doses of chemical products. However some insecticides employed in maize seed dressing, namely belonging to the neonicotinoid family and fipronil, have been claimed to play a role in the decline of honeybees (Apis mellifera L.). Pneumatic drills used in maize sowing are charged with contributing to the dispersion of the abrasion dust produced by dressed seeds, favoring the contamination of the honeybee habitat. Different devices similar to air deflectors have been introduced on pneumatic drills in order to reduce dust drift. During previous field tests carried out by the authors during recent years reduction of dust concentration both in the air and at soil surface has been shown as a consequence of their application. As field tests are affected by the variability of environmental parameters (namely wind speed and direction) the results are not always reliable, comparable and of a general validity. This paper refers to a sowing simulation test system in which pneumatic drills can be tested at a fixed point under controlled conditions of the main environmental parameters. In the test area, protected by external influences, artificial wind conditions are created by means of a fan. The drill, suitably placed in the test area, operates the seed distribution "sur place" by means of an electric engine connected to the drill's driving wheel. A 22.5 m long sampling area, leeward with respect to the drill position, has been identified. Along the sampling area a series of Petri dishes has been placed, with the aim of capturing the depositing dust and providing the concentration of the active ingredients (a.i.) at ground level. At the same time, three air samplers with PTFE diskette filters have been used for the detection of the a.i. The test system has been used for the test of a pneumatic drill, equipped with and without air deflectors, using maize seed dressed with four a. i. (imidacloprid, clothianidin, thiametoxam, fipronil). The results showed regularly decreasing of the concentrations as distance increased, both in the air and at ground level. Moreover, the difference determined by the adoption of the drift reducing device (air deflectors) resulted clear and it can be quantified at around 50 % of the a.i. amounts observed without deflectors. Finally, the paper proposes a data processing method that, from the values observed at fixed point, provides the theoretical a.i. concentration behavior that would occur in field, under the same conditions of wind speed and direction and working speed. The obtained results are coherent with previous field test.     

Redefining and mapping global irreplaceability

Irreplaceability is a concept used to describe how close a site is to being essential for achieving conservation targets. Current methods for measuring irreplaceability are based on representative combinations of sites, giving them an extrinsic nature and exponential computational requirements. Surrogate measures based on efficiency (complementarity) are often used as alternatives, but they were never intended for this purpose and do not measure irreplaceability. Current approaches used to estimate irreplaceability have key limitations. Some of these are a result of the tools used, but some are due to the nature of the current definition of irreplaceability. For irreplaceability to be stable and useful for conservation purposes and to resolve limitations, irreplaceability measures should adhere to five axioms; baseline coherence, monotonic responsiveness, proportional responsiveness, intrinsic stability, and bounded outputs. We designed a robust method for measuring a site's proximity to irreplaceability that adheres to these requirements and used it to develop the first systematic global map of irreplaceability based on data for terrestrial vertebrates (n = 29,837 species, >1 million grid cells). At least 3.5% of land surface was highly irreplaceable, and 47.6% of highly irreplaceable cells were contained in 12 countries. More generous thresholds of irreplaceability flag greater portions of land surface that would still be realistic to protect under current global objectives. Irreplaceable sites should form a critical component of any global conservation plan and should be part of the UN Convention on Biological Diversity's post2020 Global Biodiversity Framework strategy, forming part of the 30% protection by 2030 target that is gaining support. The reliable identification of irreplaceable sites will be crucial to halting extinctions.