环介导等温扩增技术(LAMP)检测人星状病毒方法的建立及其在再生水检测中的应用

为快速高效地检测人星状病毒,建立了一种新的试验方法——环介导等温扩增技术(loop-mediated isothermal amplification technology,LAMP),并对人星状病毒LAMP反应条件进行了优化.结果表明,镁离子终浓度为4 mmol·L-1、甜菜碱终浓度为1mol.L-1的25μL体系下...     

类二噁英多氯联苯生殖毒性的研究进展

类二噁英多氯联苯(DlPCBs)作为典型的环境内分泌干扰物,会干扰接触个体或其后代的内分泌功能,破坏机体的神经系统、免疫系统,尤其对生殖系统的影响最为明显,比如对生殖器官的形态与功能、生殖内分泌、原始生殖细胞及其受精和早期胚胎发育等产生严重影响,最终导致各种生殖系统疾病的发生。DlPCBs的蓄积性及半挥发性使其在环境中分布广泛,半衰期长的特点使其将对动物及人类的生存和健康造成难以想象的影响。本文综述了DlPCBs的分类与构成、一般性质、生殖毒性以及其可能的致病机理,并对DlPCBs生殖毒性机理研究的重点方向进行了展望。     

Assessment of spatial distribution of soil loss over the upper basin of Miyun reservoir in China based on RS and GIS techniques

Soil conservation planning often requires estimates of the spatial distribution of soil erosion at a catchment or regional scale. This paper applied the Revised Universal Soil Loss Equation (RUSLE) to investigate the spatial distribution of annual soil loss over the upper basin of Miyun reservoir in China. Among the soil erosion factors, which are rainfall erosivity (R), soil erodibility (K), slope length (L), slope steepness (S), vegetation cover (C), and support practice factor (P), the vegetative cover or C factor, which represents the effects of vegetation canopy and ground covers in reducing soil loss, has been one of the most difficult to estimate over broad geographic areas. In this paper, the C factor was estimated based on back propagation neural network and the results were compared with the values measured in the field. The correlation coefficient (r) obtained was 0.929. Then the C factor and the other factors were used as the input to RUSLE model. By integrating the six factor maps in geographical information system (GIS) through pixel-based computing, the spatial distribution of soil loss over the upper basin of Miyun reservoir was obtained. The results showed that the annual average soil loss for the upper basin of Miyun reservoir was 9.86 t ha(-1) ya(-1) in 2005, and the area of 46.61 km(2) (0.3%) experiences extremely severe erosion risk, which needs suitable conservation measures to be adopted on a priority basis. The spatial distribution of erosion risk classes was 66.9% very low, 21.89% low, 6.18% moderate, 2.89% severe, and 1.84% very severe. Thus, by using RUSLE in a GIS environment, the spatial distribution of water erosion can be obtained and the regions which susceptible to water erosion and need immediate soil conservation planning and application over the upper watershed of Miyun reservoir in China can be identified.     

黄土丘陵沟壑区典型植物耐旱生理及抗旱性评价

通过实地采样获取黄土高原丘陵区柠条、侧柏、油松、山杏和山毛桃5种常见植物类型的4个耐旱指标(丙二醛含量、叶绿素含量、谷胱甘肽含量、束缚水/自由水含量比值)和2个抗旱指标(脯氨酸含量、可溶性糖含量)数据,采用模糊数学隶属函数法和灰色关联分析法评价不同植物的抗旱能力和不同指标的指示效果.结果表明,不同树种的上述6个生理指标对干旱条件的响应存在显著差异,但基于单个指标获得的树种抗旱能力排序结果不完全一致,说明单个指标无法全面描述树种的抗旱能力,对5个树种抗旱能力大小的综合评价结果为柠条＞侧柏＞油松＞山杏＞山毛桃.耐旱指标叶绿素含量、束缚水/自由水含量比值、谷胱甘肽含量和抗旱指标脯氨酸含量与抗旱隶属度的关联度值均＞0.60,可用以指示植物的抗旱能力,其中,以脯氨酸含量的指示效果最好.     

大断面/中断面、长距离隧道施工通风特性及风险分析

针对上海崇明越江遂道(大断面)及青草沙原水工程越江(中断面)超长距离隧道,基于通风的有关理论建立物理数学模型,利用有限体积方法和SIMPLE算法,研究隧道施工中盾构机掘进面附近流场的湍流特性.参照隧道内作业环境标准,讨论大断面隧道掘进面环境温度较高的情况与中断面隧道发生有害气体意外泄漏的事故,分析相应的通风方案对盾构机工作区域热量释放及对有害气体扩散的控制.     

单兵用主动式听力保护技术研究现状分析

战场噪声是造成单兵听力损伤的重要原因,通常要求单兵处于大于85dBA的噪声环境中需佩戴听力保护装置。听力保护装置通常包括耳罩式和耳塞式。新型单兵用耳塞式听力保护装置具有听力保护、环境声音的监听、持续及脉冲噪声抑制、通信接入等多种功能。     

Development of a Local-Scale Spatially Refined Multimedia Fate Model (LSRMFM) for Urban-Scale Risk Assessment: Model Formulation, GIS-Based Preprocessing, and Case Study

A local-scale spatially refined multimedia fate model (LSRMFM) was developed to evaluate in detail the multimedia transport of organic compounds at a spatial level. The model was derived using a combination of an advection?Cdispersion?Creaction partial differential equation, a steady-state multimedia fugacity model, and a geographical information system. The model was applied to predicting four major volatile organic compounds that are produced as emissions (benzene, toluene, xylene, and styrene) in an urban and industrial area (the 50?×?50-km area was divided into 0.5?×?0.5-km segments) in Korea. To test the accuracy of the model, the LSRMFM was used to predict the extent of dispersion and the data compared with actual measured concentrations and the results of a generic multimedia fate model (GMFM). The results indicated that the method developed herein is appropriate for predicting long-term multimedia pollution. However, the comparison study also illustrated that the developed model has some limitations (e.g., steady-state assumption) in terms of explaining all the observed concentrations, and additional verification and study (e.g., validation using a large observed data set, integration with a more accurate runoff model) would be desirable. In comparing LSRMFM and GMFM, discrepancies between the LSRMFM and GMFM outputs were found, as the result of geographical effects, even though the environmental parameters were identical. The geographical variation for LSRMFM output indicated the existence of considerable local human and ecological risks, whereas the GMFM output indicated less average risk. These results demonstrate that the model has the potential for improving the management of pollutant levels under these refined spatial conditions.     

长潭水库富营养化现状及防治对策

介绍了长潭水库的水文、功能及社会环境概况。跟踪统计分析了3个点位9年以来的监测数据和环境质量,研究显示,总体水质先降后升,水文因素非常明显。目前富营养化程度为中营养,主要污染因子波动指标为TP、TN、叶绿素a、pH值几个项目。同时分析了污染因子波动的原因以及氮、磷的来源。根据当地环境、经济状况,提出了控制水体富营养化的建议、方法。     

2000-2007年省级区域自然灾害灾情分析

自然灾害是严重影响人民正常生产生活、国家经济持续发展的突发公共事件。区域自然灾害评估对于做好防灾减灾工作有着重要的意义。对区域灾情进行了综合评估,归纳总结了区域灾情分布规律。选择受灾人口、死亡人口、紧急转移安置人口、农作物受灾面积、农作物绝收面积、倒塌房屋、损坏房屋和直接经济损失等8个基本指标参与区域灾情评估,并利用灾情综合指数法构建了灾情指数,其中绝对指数反映了区域灾情的绝对强度,相对指数反映了区域灾情对当地社会经济的影响程度。用灾情指数综合评估了我国东、中、西部三大地带以及省级区域的灾情强度和空间分布规律,认为西部区域受灾程度最大,自然灾害对其的影响程度最深,其次是中部地区和东部地区;文章利用综合灾情的绝对指数和相对指数的对应关系,将我国大陆地区的31个省级单元划分为4类地区,即灾情总量较大且对本地影响较大、灾情总量较大但对本地影响较小、灾情总量较小但对本地影响较大以及灾情总量较小且对本地影响较小等4种类型。文章给出的灾情区域分布规律符合灾害系统理论,特别反映了承灾体的暴露度、脆弱性和抗灾能力是区域灾情强度的决定因素。评估结果与区域实际情况相符,说明所给出的灾情指数评估法是评估区域灾情强弱的合理有效方法。     

生物炭施用对紫色土旱坡地土壤氮流失形态及通量的影响

明确生物炭施用对紫色土旱坡地土壤氮流失形态及通量的影响,为提升紫色土旱坡地耕地质量及减少紫色土旱坡地农业面源污染发生风险提供科学依据.以油菜/玉米轮作农田生态系统为研究对象,通过田间试验,研究了不施肥(对照)、常规施肥、优化施肥及生物炭(化肥减量配施生物炭)这4个处理对紫色土旱坡地地表径流和壤中流氮素流失形态及通量的影响.结果表明:①在各施肥处理中,常规处理总径流量最大,为16 133 L·a~(-1),生物炭处理总径流量最小,为11 893 L·a~(-1).各施肥处理以壤中流为主要径流方式,壤中流流失量占总流失量的61.80%～68.60%;与对照(不施肥处理)相比,其余各施肥处理泥沙流失量均有所降低,其中常规处理降低的效果最明显.②铵态氮主要通过地表径流流失,占总流失通量的86.51%～96.58%;铵态氮流失通量最大的为施生物炭处理[0.69 kg·(hm~2·a)~(-1)].③各施肥处理产流中的颗粒态氮浓度均高于对照处理,且常规施肥处理的颗粒态氮流失通量最大,为2.87 kg·(hm~2·a)~(-1).④各施肥处理的壤中流和地表径流中的全氮浓度和硝态氮浓度均存在极显著正相关关系(P0.01).硝态氮是全氮流失的主要形态,且二者均以壤中流为主要流失途径;全氮通过壤中流流失占比为72.86%～89.13%,且常规施肥处理的全氮总流失通量最大,为35.58 kg·(hm~2·a)~(-1),而施生物炭处理全氮总流失通量最小,为21.49 kg·(hm~2·a)~(-1).化肥减量配施生物炭能明显降低径流量和氮的流失通量,可有效阻控农业面源污染发生的风险.