Gentamicin (Gent) is an aminoglycoside antibiotic being used in livestock sector. Gent residues could cause some genetic disorders by nonsense mutations. This study aimed to develop IgY-based ELISA for the detection of Gent in animal products. Gent was conjugated with Bovine serum albumin (BSA) by carbodiimide method for further immunization in the laying chickens. PEG-6000 extraction method was employed to extract IgY from the egg yolk. The titer of anti-Gent-IgY attained the peak of 1:256,000 after the 5th booster immunization. Checkerboard titration confirmed that, anti-Gent IgY in 1:2,000 dilution could give an Optical Density (OD) 1.0 at 2 µg mL?1 of Gent-OVA coating concentration. IgY-based indirect competitive ELISA (Ic-ELISA) showed that, the IC50 value of anti-Gent IgY was 2.69 ng mL?1 and regression curve equation was y = ?16.27x + 56.97 (R2 = 0.95, n = 3), confirming that, the detection limit (LOD, IC10 value) was 0.01 ng mL?1. Recoveries from fresh milk, pork and chicken samples were ranged from 69.82% to 94.32%, with relative standard deviation lower than 10.88%. Our results suggested that generated anti-Gent IgY antibodies can be used in routine screening analysis of Gent residues in food samples. 相似文献
Environmental Science and Pollution Research - This study analyzed the determinants that impact innovation on offshore wind energy (OSW) for a select group of countries, applying mixed-method... 相似文献
In present study, an efficient ternary Ag/TiO2/mesoporous g-C3N4 (M-g-C3N4) photocatalyst was successfully synthesized through depositing Ag nanoparticles (NPs) on the surface of TiO2/M-g-C3N4 heterojunction. Ag/TiO2/M-g-C3N4 nanocomposite displayed the highest degradation efficiency for amoxicillin (AMX) compared to TiO2/M-g-C3N4 heterojunction, M-g-C3N4, and bulk-g-C3N4 (B-g-C3N4). The removal efficiency of AMX in real situation, surface water (SW), hospital wastewater (HW), and waste water treatment plant (WWTP) also were studied to illustrate the effectiveness of Ag/TiO2/M-g-C3N4 photocatalysts. The vulnerable atoms in AMX structure were revealed through DFT calculation. Additionally, the dominating active groups produced in time of the photocatalytic procedure were determined on account of free radical trapping experiments and ESR spectra. The mechanism of photocatalytic degradation was proposed and verified. The transfer of the electrons and the inhibition of the recombination of photogenerated electron-holes were enhanced effectively under the synergistic effect of the Ag NPs and TiO2. As a consequence, the catalytic activity of the composite was improved under visible light.