Reducing atrazine losses: water quality implications of alternative runoff control practices

Water quality is being affected by herbicides, some allegedly harmful to human health. Under scrutiny is atrazine (1-chloro-3-ethylamino-5-isopropylamino-2,4,6-triazine), a commonly used herbicide in corn (Zea mays L.) and sorghum [Sorghum bicolor (L.) Moench] production. Concentrations of soluble and adsorbed atrazine losses sometimes exceed the safe drinking water standard of 3 microg L(-1) established by the USEPA. This study assesses the protective implications of runoff control structures and alternative crop farming practices to minimize atrazine losses. Using a computerized simulation model, APEX, the following four practices were the most effective with respect to the average atrazine loss as a percent of the amount applied: (i) constructing sediment ponds, 0.09%; (ii) establishing grass filter strips, 0.14%; (iii) banding a 25% rate of atrazine, 0.40%; and (iv) constructing wetlands, 0.45%. Other atrazine runoff management options, including adoption of alternative tillage practices such as conservation and no-till as well as splitting applications between fall and spring, were marginally effective.     

Overexpression of ATP sulfurylase in Indian mustard: effects on tolerance and accumulation of twelve metals

Indian mustard [Brassica juncea (L.) Czern.] transgenics overexpressing ATP sulfurylase (APS plants) were shown previously to have higher levels of total thiols, S, and Se. The present study explores the effect of ATP sulfurylase overexpression on tolerance and accumulation of other metals, both oxyanions and cations, reasoning that some anions may react directly with ATP sulfurylase, while other ions may be bound by its thiol end products. The APS transgenics were compared with wild-type plants with respect to tolerance and accumulation of As, Cd, Cr, Cu, Hg, Mn, Mo, Ni, Pb, V, W, and Zn, supplied individually in agar medium (seedlings) or in hydroponics (mature plants). At the seedling stage, APS transgenics were more tolerant than wild type to As(III), As(V), Cd, Cu, Hg, and Zn, but less tolerant to Mo and V. The APS seedlings had up to 2.5-fold higher shoot concentrations of As(III), As(V), Hg, Mo, Pb, and V, and somewhat lower Cr levels. Mature APS plants contained up to 2.5-fold higher shoot concentrations of Cd, Cr, Cu, Mo, V, and W than wild type. They also contained 1.5- to 2-fold higher levels of the essential elements Fe, Mo, and S in most of the treatments. Mature APS plants showed no differences in metal tolerance compared with the wild type. Overexpression of ATP sulfurylase may be a promising approach to create plants with enhanced phytoextraction capacity for mixtures of metals.     

Assessment of manure phosphorus export through turfgrass sod production in Erath County, Texas

A best management practice (BMP) for exporting manure phosphorus (P) in turfgrass sod from the North Bosque River (NBR) watershed in central Texas was assessed using a geographic information system (GIS). The NBR watershed has a mandate to reduce the total annual P load to the NBR by 50% as a result of total maximum daily load regulation. Since dairy waste applications to fields are identified as the major nonpoint source of P to the river, innovative BMPs, such as export of manure P in turfgrass, will be needed to achieve the 50% reduction. However, methods are needed to evaluate the feasibility of these innovative management practices prior to their implementation. A geospatial database of suitable turfgrass production sites was developed for Erath County using GIS. Erath County largely encompasses the upper portion of the NBR watershed. Information from field experiments, production practices, and ground-truthing was used to search, analyze, and verify a geospatial database developed from national and regional sources. The integration and analyses of large databases supports the search by turf producers for sites suitable for turfgrass sod production in Erath County. In addition, GIS enables researchers and regulators to estimate manure P exports and reduced P loading due to implementation of the manure export BMP on a county scale. Under optimal conditions 198,000 kg manure P yr(-1) could be used and 114,840 kg manure P yr(-1) exported from the NBR watershed through implementation of a system using dairy manure to produce turfgrass sod. This is the equivalent of the manure P applied from 10,032 dairy cows yr(-1) and exported from 5808 dairy cows yr(-1). Application of GIS to large-scale planning and decision-making transcends traditional field-scale applications in precision agriculture.     

Constructing land-use maps of the Netherlands in 2030

The National Environmental Assessment Agency of the RIVM in the Netherlands is obliged to report on future trends in the environment and nature every 4 years. The last report, Nature Outlook 2, evaluated the effects of four alternative socio-economic and demographic scenarios on nature and the landscape. Spatially detailed land-use maps are needed to assess effects on nature and landscape. The objective of the study presented here was how to create spatially detailed land-use maps of the Netherlands in 2030 using the Environment Explorer, a Cellular Automata-based land-use model to construct land-use maps from four scenarios. One of these is discussed in great detail to show how the maps were constructed from the various scenario elements, story lines and additional data and assumptions on national, regional and local land-use developments. It was the first time in the history of our outlooks that consistent, spatially detailed land-use maps of the Netherlands for 2030 were constructed from national economic and demographic scenarios. Each map represents a direct reflection of model input and assumptions. The maps do not show the most probable developments in the Netherlands but describe the possible change in land use if Dutch society were to develop according to one of the four scenarios. The large (societal) uncertainties are reflected in the total set of future land-use maps. The application of a land-use model such as the Environment Explorer ensures that all relevant aspects of a scenario, i.e. economic and demographic developments, zoning policies and urban growth, are integrated systematically into one consistent framework.     

Anatomical and experimental evidence for particulate feeding in<Emphasis Type="Italic"> Lucinoma aequizonata</Emphasis> and<Emphasis Type="Italic"> Parvilucina tenuisculpta</Emphasis> (Bivalvia: Lucinidae) from the Santa Barbara Basin

Previous nutritional models for adults of the lucinid bivalve Lucinoma aequizonata contend that symbiotic chemoautotrophic bacteria provide most of the organic carbon for the host. The existence of this symbiosis, coupled with the hosts distinctive anatomical features, shaped the impression that particulate feeding was not a significant part of L. aequizonata nutrition. Here, we use several techniques to show that particulate feeding is a consistent and important part of the L. aequizonata nutritional strategy. Histological and scanning electron microscopy observations reveal that the gills of L. aequizonata, like those of the lucinid Parvilucina tenuisculpta, have functional mucociliary epithelia, able to transport captured particles to the mouth. Observations of gut content and radiolabeled feeding experiments indicate that L. aequizonata does ingest and assimilate carbon from particulate organic matter. Furthermore, molecular identification of a broad spectrum of organisms in the guts of native adult specimens demonstrates that L. aequizonata is non-selective when ingesting organic material, and has a mixotrophic diet.Electronic Supplementary Material Supplementary material is available in the online version of this article at Communicated by P.W. Sammarco, Chauvin     

Patterns in swimming by a scyphomedusa: a novel approach to quantifying behavior in individuals

Behavior is commonly studied at the group level using several individuals, but there is increasing evidence that the behavior of a few individuals often has a disproportionate effect on the response of a population to its environment. The present study used a suite of statistical techniques, random series analysis, analysis of variance, spectral analysis, and goodness-of-fit tests of frequency histograms, to quantitatively describe the time-dependent changes in individual behavior. Each technique reveals a different facet of the behavior and, when simultaneously applied to the data, distinguishes significant differences among the behaviors of several individuals. The approach was developed and tested on the swimming behavior of four specimens of the scyphomedusa Aurelia aurita (Linnaeus, 1758), which were observed for 19 days, beginning 16 January 1998, and videotaped under identical environmental conditions during that period. The analyses showed that each medusa swam in a unique pattern, varying swimming at characteristic frequencies. Application of the approach to individual-based numerical modeling, to the role of endogenous stimuli in the behavioral repertoire, and to in situ studies of animal behavior is discussed.Communicated by J.P. Grassle, New Brunswick     

Cellular energy allocation in zebra mussels exposed along a pollution gradient: linking cellular effects to higher levels of biological organization

Organisms exposed to suboptimal environments incur a cost of dealing with stress in terms of metabolic resources. The total amount of energy available for maintenance, growth and reproduction, based on the biochemical analysis of the energy budget, may provide a sensitive measure of stress in an organism. While the concept is clear, linking cellular or biochemical responses to the individual and population or community level remains difficult. The aim of this study was to validate, under field conditions, using cellular energy budgets [i.e. changes in glycogen-, lipid- and protein-content and mitochondrial electron transport system (ETS)] as an ecologically relevant measurement of stress by comparing these responses to physiological and organismal endpoints. Therefore, a 28-day in situ bioassay with zebra mussels (Dreissena polymorpha) was performed in an effluent-dominated stream. Five locations were selected along the pollution gradient and compared with a nearby (reference) site. Cellular Energy Allocation (CEA) served as a biomarker of cellular energetics, while Scope for Growth (SFG) indicated effects on a physiological level and Tissue Condition Index and wet tissue weight/dry tissue weight ratio were used as endpoints of organismal effects. Results indicated that energy budgets at a cellular level of biological organization provided the fastest and most sensitive response and energy budgets are a relevant currency to extrapolate cellular effects to higher levels of biological organization within the exposed mussels.     

A review of hypotheses for the functions of avian duetting

Avian duets are striking for the remarkable precision with which duetting partners sometimes coordinate their songs. Duetting species are taxonomically diverse, and the form of their duets varies. The reasons some birds duet when most do not remains unclear despite numerous hypotheses for its function. I review work done so far on duetting, discuss evidence for and against hypotheses for its functions, and highlight approaches useful for future research. The four hypotheses that appear most promising are that individuals join their partners songs to form duets: (1) to avoid being usurped from a partnership, (2) to prevent their partner being usurped, (3) as a collaborative display in defence of some resource, or (4) to signal commitment to their partner. These hypotheses are not mutually exclusive, and duetting is likely to have multiple roles both within and among species. However, much basic research is still required. Characteristics of duets have rarely been quantified in detail, and information about variability among species in the precision of duetting is necessary, not only to test hypotheses about function, but also to define duetting more precisely. Quantifying the relative frequencies of alternative vocal strategies (for example, remaining silent when a partner sings versus joining in to form a duet) between species and in different contexts will help to determine why partners coordinate their songs to form duets. Furthermore, social systems and sex roles in duetting species are poorly understood, yet understanding these is critical to determining the functions of avian duetting.Communicated by A. Cockburn     

Using Multimedia Modeling to Expedite Site Characterization

GOAL, SCOPE AND BACKGROUND: This paper uses two case studies of U.S. Department of Energy nuclear weapons complex installations to illustrate the integration of expedited site characterization (ESC) and multimedia modeling in the remedial action decision making process. CONCEPTUAL SITE MODELS, MULTIMEDIA MODELS, AND EXPEDITED SITE CHARACTERIZATION: Conceptual site models outline assumptions about contaminates and the spatial/temporal distribution of potential receptors. Multimedia models simulate contaminant transport and fate through multiple environmental media, estimate potential human exposure via specific exposure pathways, and estimate the risk of cancer and non-cancer health outcomes. ESC relies on using monitoring data to quantify the key components of an initial conceptual site model that is modified iteratively using the multimedia model. CASE STUDIES: Two case studies are presented that used the ESC approach: Los Alamos National Laboratory (LANL) and Pantex. LANL released radionuclides, metals, and organic compounds, into canyons surrounding the facility. The Pantex Plant has past waste management operations which included burning chemical wastes in unlined pits, burying wastes in unlined landfills, and discharging plant wastewaters into on-site surface waters. CONCLUSIONS: The case studies indicate that using multimedia models with the ESC approach can inform assessors about what, where, and how much site characterization data needs to be collected to reduce the uncertainty associated with risk assessment. Lowering the degree of uncertainty reduces the time and cost associated with assessing potential risk and increases the confidence that decision makers have in the assessments performed.     

Environmental impact of unleaded gasolines in the bay of Cádiz (Spain)

Given the strategic situation of the José León de Carranza bridge, which spans the Bay of Cádiz (in the SW of Spain) and carries very heavy motor traffic, together with knowledge of the currents and tidal flows in the zone, we have used a technique of radioactive dating of sediments to study the temporal evolution presented by contamination from lead in the sediment column. This has allowed us to observe the environmental impact, in terms of the concentration of Pb in the sea bed sediments, that has been produced in the zone by the introduction of unleaded gasolines as substitutes for traditional automobile fuels that employ organic forms of tetra methyl lead as an antidetonant agent in the fuel.