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61.
Preliminary evidence of the role of hydrogen peroxide in the degradation of benzo[a]pyrene by a non-white rot fungus Fusarium solani 总被引:1,自引:0,他引:1
Veignie E Rafin C Woisel P Cazier F 《Environmental pollution (Barking, Essex : 1987)》2004,129(1):1-4
In order to study the enzymatic mechanisms involved in the successive steps of BaP degradation by a Deuteromycete fungus Fusarium solani, we developed an indirect approach by using inhibitors of enzymes. We used either specific inhibitors of peroxidases (i.e. salicylhydroxamic acid) and of cytochrome P-450 (i.e. piperonyl butoxyde) or inhibitors of both enzymes (i.e. potassium cyanide). Surprisingly, no expected decrease of BaP degradation was observed with most inhibitors tested. On the contrary, more BaP was degraded. Only butylated hydroxytoluene, which acts as a free radical scavenger, inhibited BaP degradation. The inhibition of these enzymes, which use H(2)O(2) as a cosubstrate, might have resulted in an increase of hydrogen peroxide availability in the fungal cultures. This enhancement could induce formation of reactive oxygen species (ROS) which might be the agents that initiate benzo[a]pyrene oxidation. This study proposed a hypothetic alternative metabolic pathway involved in PAH metabolism by Fusarium solani. 相似文献
62.
In darkness, hamsters commute between their nest and a feeding site through path integration only, and therefore show cumulative
errors in the return direction to the nest. We examined whether a brief presentation of familiar room cues could reset the
path integrator. The hamsters could see the room cues either during, or at the end of, the outward journey to the food place,
in a conflict situation where motion cues and visual information were set at variance. In both conditions, the animals used
mainly visual information to return home. Thus, hamsters can determine their azimuth, and possibly their location, through
a visual fix, and can reset their path integrator through the fix. This allows them to update their position during further
locomotion in the dark and thus to compute a correct homing vector with respect to a visually induced reference frame. Taking
episodic positional fixes may greatly enhance the functional value of path integration.
Received: 17 May 2000 / Accepted in revised form: 11 September 2000 相似文献