Natural products may be applied in a wide range of domains, from agriculture to food and pharmaceutical industries. In this study, the antioxidant properties and the capacity to inhibit some enzymatic activities of Euphorbia resinifera and Euphorbia officinarum aqueous extracts and honeys were assessed. The physicochemical characteristics were also evaluated. Higher amounts of iron, copper and aluminium were detected in E. officinarum honey, which may indicate environmental pollution around the beehives or inadequate storage of honey samples. This honey sample showed higher amounts of total phenols and better capacity for scavenging superoxide anion free radicals and DPPH free radicals as compared with E. resinifera honey, but poorer capacity for inhibiting lipoxygenase, acetylcholinesterase, tyrosinase and xanthine oxidase. The ratio plant mass:solvent volume (1:100) and extraction time (1 - 2 h) were associated with higher total phenols and better antioxidant activities and lipoxygenase, acetylcholinesterase and tyrosinase inhibitory activities, regardless of the plant species. The aqueous extracts had systematically higher in vitro activities than the respective honey samples.
Environmental Science and Pollution Research - This work is focused on the design and preparation of polymer inclusion membranes (PIMs) for potential applications for stannous cation sequestration... 相似文献
Per-and polyfluoroalkyl substances(PFASs) are ubiquitous in sludge and water from waste water treatment plants, as a result of their incorporation in everyday products and industrial processes. In this study, we measured several classes of persistent PFASs,precursors, transformation intermediates, and newly identified PFASs in influent and effluent sewage water and sludge from three municipal waste water treatment plants in Sweden, sampled in 2015. For sludge, samples from 2012 and 2014 were analyzed as well.Levels of precursors in sludge exceeded those of perfluoroalkyl acids and sulfonic acids(PFCAs and PFSAs), in 2015 the sum of polyfluoroalkyl phosphoric acid esters(PAPs) were 15–20 ng/g dry weight, the sum of fluorotelomer sulfonic acids(FTSAs) was 0.8–1.3 ng/g,and the sum of perfluorooctane sulfonamides and ethanols ranged from non-detected to 3.2 ng/g. Persistent PFSAs and PFCAs were detected at 1.9–3.9 ng/g and 2.4–7.3 ng/g dry weight, respectively. The influence of precursor compounds was further demonstrated by an observed substantial increase for a majority of the persistent PFCAs and PFSAs in water after waste water treatment. Perfluorohexanoic acid(PFHxA), perfluorooctanoic acid(PFOA), perfluorohexane sulfonic acid(PFHxS), and perfluorooctane sulfonic acid(PFOS)had a net mass increase in all WWTPs, with mean values of 83%, 28%, 37% and 58%,respectively. The load of precursors and intermediates in influent water and sludge combined with net mass increase support the hypothesis that degradation of precursor compounds is a significant contributor to PFAS contamination in the environment. 相似文献
Norovirus (NoV) is recognized as the most common cause of foodborne outbreaks. In 2014, an outbreak of acute gastroenteritis occurred on a cruise ship in Brazil, and NoV became the suspected etiology. Here we present the molecular identification of the NoV strains and the use of sequence analysis to determine modes of virus transmission. Food (cream cheese, tuna salad, grilled fish, orange mousse, and vegetables soup) and clinical samples were analyzed by ELISA, conventional RT-PCR, qRT-PCR, and sequencing. Genogroup GII NoV was identified by ELISA and conventional RT-PCR in fecal samples from 5 of 12 patients tested (41.7%), and in the orange mousse food sample by conventional RT-PCR and qRT-PCR. Two fecal GII NoV samples and the orange mousse GII NoV sample were successfully genotyped as GII.Pe (ORF 1), revealed 98.0–98.8% identities among them, and shared phylogenetically distinct cluster. Establishing the source of a NoV outbreak can be a challenging task. In this report, the molecular analysis of the partial RdRp NoV gene provided a powerful tool for genotyping (GII.Pe) and tracking of outbreak-related samples. In addition, the same fast and simple extraction methods applied to clinical samples could be successfully used for complex food matrices, and have the potential to be introduced in routine laboratories for screening foods for presence of NoV. 相似文献
We reviewed existing and planned adaptation activities of federal, tribal, state, and local governments and the private sector in the United States (U.S.) to understand what types of adaptation activities are underway across different sectors and scales throughout the country. Primary sources of review included material officially submitted for consideration in the upcoming 2013 U.S. National Climate Assessment and supplemental peer-reviewed and grey literature. Although substantial adaptation planning is occurring in various sectors, levels of government, and the private sector, few measures have been implemented and even fewer have been evaluated. Most adaptation actions to date appear to be incremental changes, not the transformational changes that may be needed in certain cases to adapt to significant changes in climate. While there appear to be no one-size-fits-all adaptations, there are similarities in approaches across scales and sectors, including mainstreaming climate considerations into existing policies and plans, and pursuing no- and low-regrets strategies. Despite the positive momentum in recent years, barriers to implementation still impede action in all sectors and across scales. The most significant barriers include lack of funding, policy and institutional constraints, and difficulty in anticipating climate change given the current state of information on change. However, the practice of adaptation can advance through learning by doing, stakeholder engagements (including “listening sessions”), and sharing of best practices. Efforts to advance adaptation across the U.S. and globally will necessitate the reduction or elimination of barriers, the enhancement of information and best practice sharing mechanisms, and the creation of comprehensive adaptation evaluation metrics. 相似文献
The purpose of this study was to demonstrate that shellfish can be used to detect enteric viruses in marine waters where they
are present at very low concentrations. Aqua-cultured mussels were placed in the sea just off the mouth of a drainage channel
affected by human and animal faecal contamination. Samples were taken from the channel, the sea and the mussels at intervals
over two 4-week periods. The samples were tested to verify the presence of both rotaviruses and E. coli. Rotaviruses were detected by Real Time-PCR, typed by multiplex PCR and subsequently sequenced. E. coli was enumerated in water matrices by a filtering method and in mussels by the MPN method. The presence of E. coli in the examined matrices demonstrates contamination of faecal origin throughout the studied environments. Rotaviruses were
recorded in channel waters, but not in sea water. In both experiments, rotaviruses were detected in mussels 21 and 28 days
after being placed in the sea water off the channel mouth. The use of mussels thus enabled the detection of rotaviruses in
waters where the high dilution rendered direct investigation impossible. This study indicates that mussels can be used in
marine virological surveillance programs. 相似文献
Recent events have shown that humans may become infected with some pathogenic avian influenza A viruses (AIV). Since soil
and water, including lakes, rivers, and seashores, may be contaminated by AIV excreted by birds, effective methods are needed
for monitoring water for emerging viruses. Combining water filtration with molecular methods such as PCR is a fast and effective
way for detecting viruses. The objective of this study was to apply a convenient method for the detection of AIV in natural
water samples. Distilled water and lake, river, and seawater were artificially contaminated with AIV (H5N3) and passed through
a filter system. AIV was detected from filter membrane by real-time RT-PCR. The performance of Zetapor, SMWP, and Sartobind
D5F membranes in recovering influenza viruses was first evaluated using contaminated distilled water. SWMP, which gave the
highest virus recoveries, was then compared with a pre-filter combined GF/F filter membrane in a trial using natural water
samples. In this study, the cellulose membrane SMWP was found to be practical for recovery of AIVs in water. Viral yields
varied between 62.1 and 65.9% in distilled water and between 1 and 16.7% in natural water samples. The borosilicate glass
membrane GF/F combined with pre-filter was also feasible in filtering natural water samples with viral yields from 1.98 to
7.33%. The methods described can be used for monitoring fresh and seawater samples for the presence of AIV and to determine
the source of AIV transmission in an outbreak situation. 相似文献
We assessed the economic suitability of 4 greenhouse gas (GHG) mitigation options and one GHG offset option for an improvement
of the GHG balance of a representative Swiss suckler cow farm housing 35 Livestock units and cultivating 25 ha grassland.
GHG emissions per kilogram meat in the economic optimum differ between the production systems and range from 18 to 21.9 kg
CO2-eq./kg meat. Only GHG offset by agroforestry systems showed the potential to significantly reduce these emissions. Depending
on the production system agroforestry systems could reduce net GHG emissions by 66% to 7.3 kg CO2-eq./kg meat in the most intensive system and by 100% in the most extensive system. In this calculation a carbon sequestration
rate of 8 t CO2/ha/year was assumed. The potential of a combination of the addition of lipids to the diet, a cover of the slurry tank and
the application of nitrification inhibitors only had the potential to reduce GHG emissions by 12% thereby marginal abatement
costs are increasing much faster than for agroforestry systems. A reduction of the GHG emissions to 7.5 kg CO2-eq./kg meat—possible with agroforestry only—raised costs between 0.03 CHF/kg meat and 0.38 CHF/kg meat depending on the production
system and the state of the system before the reduction. If GHG emissions were reduced maximally average costs ranged between
0.37 CHF/kg meat, if agroforestry had the potential to reduce net GHG emissions to 0 kg CO2-eq., to 1.17 CHF/kg meat if also other options had to be applied. 相似文献
Time series analysis has been used to evaluate the mechanisms regulating population dynamics of mammals and insects, but has
been rarely applied to amphibian populations. In this study, the influence of endogenous (density-dependent) and exogenous
(density-independent) factors regulating population dynamics of the terrestrial plethodontid salamander Speleomantes strinatii was analysed by means of time series and multiple regression analyses. During the period 1993–2005, S. strinatii population abundance, estimated by a standardised temporary removal method, displayed relatively low fluctuations, and the
autocorrelation function (ACF) analysis showed that the time series had a noncyclic structure. The partial rate correlation
function (PRCF) indicated that a strong first-order negative feedback dominated the endogenous dynamics. Stepwise multiple
regression analysis showed that the only climatic factor influencing population growth rate was the minimum winter temperature.
Thus, at least during the study period, endogenous, density-dependent negative feedback was the main factor affecting the
growth rate of the salamander population, whereas stochastic environmental variables, such as temperature and rainfall, seemed
to play a minor role in regulation. These results stress the importance of considering both exogenous and endogenous factors
when analysing amphibian long-term population dynamics. 相似文献