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Stephan Jänsch Johannes Bauer David Leube Mathias Otto Jörg Römbke Hanka Teichmann Karolina Waszak 《Environmental Sciences Europe》2018,30(1):38
Background
Few suitable and standardized test methods are currently available to test the effects of genetically modified plants (GMP) on non-target organisms. To fill this gap and improve ecotoxicological testing for GMP, we developed a new soil ecotoxicological test method using sciarid larvae as test organisms.Results
Bradysia impatiens was identified as a candidate species. Species of the genus Bradysia occur in high numbers in European agroecosystems and B. impatiens can be reared in the laboratory in continuous culture. A functional basic test design was successfully developed. Newly hatched larvae were used as the initial life stage to cover most of the life cycle of the species during the test. Azadirachtin was identified as a suitable reference substance. In several tests, the effects of this substance on development time and emergence rate varied for different temperatures and test substrates. The toxicity was higher at 25 °C compared to 20 °C and in tropical artificial soil compared to coconut fiber substrate.Conclusions and outlook
Results suggest that the developed test system is suitable to enter a full standardization process, e.g., via the Organisation for Economic Co-operation and Development. Such a standardization would not only assist the risk assessment of GMP, but could include other stressors such as systemic pesticides or veterinary pharmaceuticals reaching the soil, e.g., via spreading manure. The use of sciarid flies as test organisms supports recommendations of EFSA, which stressed the ecological role of flies and encouraged including Diptera into test batteries.13.
Monitoring pollution in River Mureş, Romania, part II: metal accumulation and histopathology in fish
Triebskorn R Telcean I Casper H Farkas A Sandu C Stan G Colărescu O Dori T Köhler HR 《Environmental monitoring and assessment》2008,141(1-3):177-188
As a part of an exposure and effect monitoring conducted along the river Mureş, Western Romania in 2004, the health status
of two indigenous fish species, sneep (Chondrostoma nasus) and European chub (Leuciscus cephalus) was investigated upstream and downstream the city of Arad. In fish, histopathology was assessed in liver and gills, and
heavy metals (cadmium, copper, lead and zinc) were analyzed in liver samples. In both fish species, histopathological reactions
in the gills (epithelial lifting, focal proliferation of epithelial cells of primary and secondary lamellae and resulting
fusion of secondary lamellae, hyperplasia and hypertrophy of mucous cells, focal inflammation and necrosis of epithelial cells)
were most severe at the two sampling sites upstream Arad city, which were shown to be polluted by copper, cadmium, faecal
coliforms and streptococci in a parallel study. At these two sites, also histopathology in the liver of L. cephalus was more prominent than at the two downstream sites. In C. nasus, symptoms in the liver (focal inflammation with lymphocytic infiltrations, macrophage aggregates and single cell necrosis)
were also highly pronounced at the sampling site located directly downstream the municipal sewage treatment plant of Arad.
With the exception of copper accumulation in L. cephalus caught at the most upstream sampling site, in both fish species cadmium and copper accumulation were exceptionally high and
did not differ significantly between the four sampling sites. 相似文献
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Assessment of the environmental significance of heavy metal pollution in surficial sediments of the River Po 总被引:13,自引:0,他引:13
The magnitude and ecological relevance of metal pollution of the middle Po river deriving from the River Lambro tributary was investigated by applying different (complementary) sediment quality assessment approaches: (1) comparisons of concentrations with regional reference data, and (2) comparisons with consensus-based sediment quality guidelines (SQGs), as well as by investigations of the partitioning patterns of target heavy metals (Cd, Cu, Ni, Pb, Zn). Total metal concentrations in the surficial sediments revealed significant pollution inputs on the whole river stretch investigated, with a distinct peak at the inlet of the River Lambro. Based on the geoaccumulation index of target heavy metals, the middle reach of River Po has to be considered as moderately polluted with Cd (1相似文献
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The Science of Nature - 相似文献
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Cousins IT Hartlieb N Teichmann C Jones KC 《Environmental pollution (Barking, Essex : 1987)》1997,97(3):229-238
A laboratory experiment was carried out to measure volatilisation fluxes of polychlorinated biphenyls (PCBs) from sewage sludge-amended soils. The most commonly practised methods of applying sludge to agricultural land in the UK, namely, surface application, ploughing in to soil and subsurface injection, were simulated inside glass experimental chambers using an anaerobically digested sludge and a sandy loam soil. Humidified air was blown over the surface of the soil/sludge in the chambers for a period of 32 days, in order to sample a sufficient air volume to detect the volatilising PCBs. The resulting PCB volatilisation fluxes from the different sludge application methods were quantified and compared. Volatilisation fluxes of individual congeners were generally highest for the surface sludge (1-cm depth) application and slightly lower for the plough layer (5-cm depth) application. Fluxes from the subsurface layer of sludge (5-cm depth) were only quantified for the lightest congeners near to the end of the experimental run-time. Results from a multiple regression analysis showed that volatilisation fluxes of PCBs from the surface application are highly dependent on both the sludge concentration and the log of the octanol-air partition coefficient (K(OA)). A well-known soil volatilisation model, developed by Jury et al., was adapted and used to predict fluxes for the different sludge application methods during the experiment. The model predicted volatilisation fluxes that were reasonably comparable to measured fluxes for some congeners, but for others predicted fluxes that were more than an order of magnitude lower than measured fluxes. The model predicted similar loss kinetics to those observed in the experiment. Possible reasons for the dissimilarity between measured and predicted fluxes include inaccuracies in model input parameters and the fact that the models were not developed for predicting fluxes from sludge-amended soils. 相似文献
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