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Kaluzhnaya OV Belikov SI Schröder HC Wiens M Giovine M Krasko A Müller IM Müller WE 《Die Naturwissenschaften》2005,92(3):134-138
In a preceding study it has been reported that the freshwater sponge Lubomirskia baicalensis, living in Lake Baikal (East Siberia), is composed of spicules forming a characteristic pattern which follows radiate accretive growth. Here we report that the spicules are synthesized by the enzyme silicatein, a protein which is related to cathepsin L. The cDNAs for silicatein and the related cathepsin L were isolated and used as probes to show that the mRNA levels of silicatein in the bases of the spicule skeleton of the animals are low, while the mRNA level of cathepsin L in this region exceeds that of the growing zone. This is the first comprehensive study on the importance of the axial filament/silicatein as an essential structural and functional component determining the growth and stability of demosponge spicules.Note: Sequences from Lubomirskia baicalensis have been deposited in the EMBL/GenBank; cDNAs for the putative silicatein (LBSILICA) are deposited under the accession no. AJ786771 and for cathepsin L (LBCATL) under the accession no. AJ786770. 相似文献
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The date when a landbird migrant arrives on its breeding grounds may have reproductive consequences. Generally, early arriving individuals begin breeding earlier and consequently experience greater seasonal reproductive performance. Here, we describe relationships between arrival timing and seasonal reproductive performance in the American redstart (Setophaga ruticilla), a long-distance passerine migrant, arriving at northerly breeding grounds in Michigans eastern Upper Peninsula. Evidence suggests that both males and females benefited from early arrival at the breeding grounds. Early males appeared to settle on higher quality territories and hatched nestlings sooner than later arrivals. Early females began their clutches early, produced heavier nestlings and possibly laid more eggs than later arrivals. Larger clutches and heavier offspring increase the likelihood of offspring recruiting into the breeding population. The findings of this study point to fitness consequences arising from when a bird arrives at its breeding grounds. These results also have implications for understanding how events occurring during spring migration influence reproductive performance as migratory delays likely influence arrival timing.Communicated by: W. Wiltschko 相似文献
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Geochemistry and bioavailability of metals in sediments from northern San Francisco Bay 总被引:7,自引:0,他引:7
In this study, metals (Be, Cr, Mn, Fe, Ni, Cu, Zn, Ag, Cd, Pb and Hg) in the fine-grained fraction (<63 microm) from 12 sites at different locations in northern San Francisco Bay over a year period from March 2000 to March 2001 were analyzed after acid extraction. The results showed that metal concentrations in the sediments varied from site to site, whereas some of them were found elevated with respect to the sediment of Tomales Bay, CA, which has little contamination history, indicating an enrichment of the metals in the sediment samples analyzed. Sediment toxicity and bioaccumulation evaluation by a clam species, Macoma nasuta, exposed to the sediment samples collected from the six sampling sites was carried out. The results showed that the sediment samples tested significantly reduced clam survival. Toxicity of the sediments to the clam was, in part, related to elevated metal concentrations in the sediments. In order to examine geochemistry of the metals and to understand potential correlations between metal concentrations and geochemical matrix elements of the sediments, bioavailability and toxicity of the metals, detailed analysis of metal concentrations associated with total organic carbon and the Fe-oxy-hydroxides in the sediment samples was performed. The analysis showed that sediment geochemistry appeared to influence metal bioavailability and may have important impacts on the toxicity of these metals to the clam. 相似文献
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OBJECTIVE: This study was undertaken to develop biomechanical corridors applicable to the small-sized female in side impacts. METHODS: Sled tests were conducted using post mortem human subjects at a velocity of 6.7 m/s. Three chestbands were used to compute deflection-time histories at the axilla, xyphoid process, and tenth rib levels. Triaxial accelerometers were fixed to the upper and lower spine and sacrum to record acceleration-time histories. Specimens contacted the load wall with varying initial conditions (rigid and padded; flat wall and offset) from which impact forces to the thoracic, abdominal, and pelvic regions were obtained using load cell data. Adopting signal processing and mass-based scaling methods, corridors were derived for forces, accelerations, and chest deflections at three levels for all initial conditions. RESULTS: All time history corridors were expressed as mean plus/minus one standard deviation and provided in the article. CONCLUSIONS: Acceleration-, deflection-, and force-time corridors obtained for the chest and pelvic regions of the human body will assist in the assessment of anthropomorphic test devices used in crashworthiness evaluations. 相似文献
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