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941.
Waled M. El-Senousy Adel Abdel-Moneim Mahmoud Abdel-Latif Mohamed H. EL-Hefnawy Rehab G. Khalil 《Food and environmental virology》2018,10(1):89-98
This study proposed to detect the enterovirus (EV) infection in children with type 1 diabetes mellitus (T1D) and to assess the role of insufficiently treated water and sewage as sources of viral spreading. Three hundred and eighty-two serum specimens of children with T1D, one hundred serum specimens of children who did not suffer from T1D as control, and forty-eight water and sewage samples were screened for EV RNA using nested RT-PCR. The number of genome copies and infectious units of EVs in raw and treated sewage and water samples were investigated using real-time (RT)-PCR and plaque assay, respectively. T1D markers [Fasting blood glucose (FBG), HbA1c, and C-peptide], in addition to anti-Coxsackie A & B viruses (CVs A & B) IgG, were measured in control, T1D-negative EV (T1D–EV?), and T1D-positive EV (T1D–EV+) children specimens. The prevalence of EV genome was significantly higher in diabetic children (26.2%, 100 out of 382) than the control children (0%, 0 out of 100). FBG and HbA1c in T1D–EV? and T1D–EV+ children specimens were significantly higher than those in the control group, while c-peptide in T1D–EV? and T1D–EV+ children specimens was significantly lower than that in the control (n = 100; p < 0.001). Positivity of anti-CVs A & B IgG was 70.7, 6.7, and 22.9% in T1D–EV+, T1D–EV?, and control children specimens, respectively. The prevalence of EV genome in drinking water and treated sewage samples was 25 and 33.3%, respectively. The prevalence of EV infectious units in drinking water and treated sewage samples was 8.5 and 25%, respectively. Quantification assays were performed to assess the capabilities of both wastewater treatment plants (WWTPs) and water treatment plants (WTPs) to remove EV. The reduction of EV genome in Zenin WWTP ranged from 2 to 4 log10, while the reduction of EV infectious units ranged from 1 to 4 log10. The reduction of EV genome in El-Giza WTP ranged from 1 to 3 log10, while the reduction of EV infectious units ranged from 1 to 2 log10. This capability of reduction did not prevent the appearance of infectious EV in treated sewage and drinking water. Plaque purification was performed for isolation of separate EV isolates from treated and untreated water and sewage samples. Characterization of the EV amplicons by RT-PCR followed by sequencing of these isolates revealed high homology (97%) with human coxsackievirus B4 (CV B4) in 60% of the isolates, while the rest of the isolates belonged to poliovirus type 1 and type 2 vaccine strains. On the other hand, characterization of the EV amplicons by RT-PCR followed by sequencing for T1D–EV+ children specimens indicated that all samples contained CV B4 with the same sequence characterized in the environmental samples. CV B4-contaminated drinking water or treated sewage may play a role as a causative agent of T1D in children. 相似文献
942.
G. Bonanno Ferraro P. Mancini M. Divizia E. Suffredini S. Della Libera M. Iaconelli G. La Rosa 《Food and environmental virology》2018,10(4):386-390
Human Cosavirus (HCoSV) is a newly discovered virus whose role in human enteric diseases is still unknown. In Italy, the prevalence and genetic diversity of HCoSV are unexplored. One hundred forty-one raw sewage samples collected throughout Italy were screened for HCoSV by RT-nested PCR. HCoSV was detected in 25.5% of samples. Species A, C, and D, and a potentially new species were detected. Our results show a significant circulation and heterogeneity of HCoSV in Italy. 相似文献
943.
944.
945.
J. Besseau-Ayasse C. Violle-Poirsier A. Bazin N. Gruchy A. Moncla F. Girard M. Till F. Mugneret A. Coussement F. Pelluard M. Jimenez P. Vago M. F. Portnoï C. Dupont C. Beneteau F. Amblard M. Valduga J. L. Bresson F. Carré-Pigeon N. Le Meur S. Tapia C. Yardin A. Receveur J. Lespinasse E. Pipiras M. P. Beaujard P. Teboul S. Brisset M. Catty E. Nowak N. Douet Guilbert H. Lallaoui S. Bouquillon V. Gatinois G. Joly-Helas F. Prieur F. Cartault D. Martin P. Kleinfinger D. Molina Gomes M. Doco-Fenzy F. Vialard 《黑龙江环境通报》2014,34(5):424-430
946.
Wolff Alexander Günther Taras Albert Thiemo Johne Reimar 《Food and environmental virology》2020,12(4):350-354
Food and Environmental Virology - Hepatitis E virus (HEV) infection can cause acute and chronic hepatitis in humans. The zoonotic HEV genotype 3, which is highly prevalent in Europe, is mainly... 相似文献
947.
948.
Werner E. G. Müller 《Die Naturwissenschaften》1998,85(1):11-25
Geodia cydonium , which code for proteins. The analyses of their deduced amino acid sequences allowed a molecular biological approach to solve
the problem of monophyly of Metazoa. Molecules of the extracellular matrix/basal lamina, with the integrin receptor, fibronectin,
and galectin as prominent examples, cell-surface receptors (tyrosine kinase receptor), elements of sensory systems (crystallin,
metabotropic glutamate receptor), and homologs/modules of an immune system (immunoglobulin like molecules, scavenger receptor
cysteine-rich, and short consensus repeats, rhesus system) classify the Porifera as true Metazoa. As living fossils, provided
with simple, primordial molecules allowing cell-cell and cell-matrix adhesion as well as processes of signal transduction
as known in a more complex manner from higher Metazoa, they also show peculiarities not known in other metazoan phyla. Tissues
of sponges are rich in telomerase activity, suggesting a high plasticity in the determination of cell lineages. It is concluded
that molecular biological studies with sponges as model will not only help to understand the evolution of Protoctista to Metazoa
but also the complex, hierarchial regulatory network of cells in higher Metazoa. 相似文献
949.
Effects of northeast monsoon on trace metal distribution in the South China Sea off Peninsular Malaysia 总被引:1,自引:0,他引:1
G. Adiana N. A. M. Shazili M. A. Marinah J. Bidai 《Environmental monitoring and assessment》2014,186(1):421-431
Concentrations of trace metals in the South China Sea (SCS) were determined off the coast of Terengganu during the months of May and November 2007. The concentrations of dissolved and particulate metals were in the range of 0.019–0.194 μg/L and 50–365 μg/g, respectively, for cadmium (Cd), 0.05–0.45 μg/L and 38–3,570 μg/g for chromium (Cr), 0.05–3.54 μg/L and 21–1,947 μg/g for manganese (Mn), and 0.03–0.49 μg/L and 2–56,982 μg/g for lead (Pb). The order of mean log K D found was Cd?>?Cr?>?Pb?>?Mn. The study suggests that the primary sources of these metals are discharges from the rivers which drain into the SCS, in particular the Dungun River, which flows in close proximity to agricultural areas and petrochemical industries. During the northeast monsoon, levels of particulate metals in the bottom water samples near the shore were found to be much higher than during the dry season, the probable result of re-suspension of the metals from the bottom sediments. 相似文献
950.
Rupali Sabale T. P. Ahammed Shabeer Sagar C. Utture Kaushik Banerjee Manjusha R. Jadhav Dasharath P. Oulkar Pandurang G. Adsule Madhukar B. Deshmukh 《Environmental monitoring and assessment》2014,186(4):2369-2374
A field dissipation study was conducted to evaluate the pre-harvest interval (PHI) and processing factor (PF) for kresoxim methyl (Ergon 44.3 SC) residues in grapes and during raisin making process at recommended dose (RD) and double the recommended dose (DRD). Kresoxim methyl residues dissipated following 1st-order kinetics with a half-life of 10 and 18 days at RD and DRD, respectively. The PHIs with respect to the European Union maximum residue limit (EU-MRL) of 1 mg kg?1 for grapes were 13 and 30 days at RD and DRD, respectively. The degradation data during grape to raisin making process were best fitted to nonlinear 1st?+?1st-order kinetics with a half-life ranging between 4 and 8 days for both shade drying and with raisin dryer at different doses. The PFs were 1.19 and 1.24 with shade drying and 1.09 and 1.10 with raisin dryer, respectively, which indicates concentration of the residues during raisin making process. The dietary exposure of kresoxim methyl on each sampling day was less than the respective maximum permissible intake both at RD and DRD. The residues of kresoxim methyl in market samples of grapes and raisins were well below the EU-MRL and were also devoid of any risk of acute toxicity related to dietary exposure. 相似文献