Effects of HMX exposure upon metabolic rate of northern bobwhite quail (Colinus virginianus) in ovo

We evaluated the use of the gas exchange rate as an ecologically relevant indicator of chemical stress in avian embryos/eggs. Northern bobwhite quail (Colinus virginianus) were exposed to octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) via feed containing nominal concentrations of 0, 12.5, 50.0, and 125.0 mg kg(-1). Metabolic rates (oxygen consumption) of developing quail eggs were then measured via respirometry to examine potential effects of HMX exposure. Metabolic rates were examined on 5, 9, and 21 d of incubation. Next, concentrations of HMX in embryos/eggs were determined by liquid chromatography-mass spectrometry. Mean (+/-SE) concentrations of HMX in eggs were 21.0+/-5.9, 1113+/-79.0, 3864+/-154.0, and 7426+/-301.1 ng g(-1) in control, low, medium and high dose groups, respectively. There were significant differences in oxygen consumption among the three embryo ages, however differences among the ages were not consistent among dose groups (age x dose group interaction p<0.0001). Oxygen consumption rates did not vary as a function of HMX in embryos (p=0.18). No evidence was observed for alterations of in ovo metabolic rates associated with HMX exposure.     

Remediation of PAH contaminated soils: application of a solid-liquid two-phase partitioning bioreactor

The feasibility of a two-step treatment process has been assessed at laboratory scale for the remediation of soil contaminated with a model mixture of polycyclic aromatic hydrocarbons (PAHs) (phenanthrene, pyrene, and fluoranthene). The initial step of the process involved contacting contaminated soil with thermoplastic, polymeric pellets (polyurethane). The ability of three different mobilizing agents (water, surfactant (Biosolve) and isopropyl alcohol) to enhance recovery of PAHs from soil was investigated and the results were compared to the recovery of PAHs from dry soil. The presence of isopropyl alcohol had the greatest impact on PAH recovery with approximately 80% of the original mass of PAHs in the soil being absorbed by the polymer pellets in 48 h. The second stage of the suggested treatment involved regeneration of the PAH loaded polymers via PAH biodegradation, which was carried out in a solid-liquid two-phase partitioning bioreactor. In addition to the PAH containing polymer pellets, the bioreactor contained a microbial consortium that was pre-selected for its ability to degrade the model PAHs and after a 14 d period approximately 78%, 62% and 36% of phenanthrene, pyrene, and fluoranthene, respectively, had been desorbed from the polymer and degraded. The rate of phenanthrene degradation was shown to be limited by mass transfer of phenanthrene from the polymer pellets. In case of pyrene and fluoranthene a combination of mass transfer and biodegradation rate might have been limiting.     

Extinction of a West African Red Colobus Monkey

Abstract: We provide the first documented case of the extinction in the twentieth century of a widely recognized primate taxon. During surveys in Ghana and Côte d'Ivoire in 1993–1999, we were unable to find any surviving populations of Miss Waldron's red colobus monkey (   Procolobus badius waldroni ), a primate taxon endemic to the forests of this part of West Africa. We conclude that this monkey, which at least one authority considers worthy of species status, is probably extinct. Hunting by humans appears to be the ultimate cause of the extinction. Until our surveys began, little attention had been paid to the plight of this red colobus monkey, despite its listing as endangered by the World Conservation Union. The extinction of other large animals in the Upper Guinea rainforest region is likely to follow soon unless more attention is paid to the full range of endangered forms and more resources are devoted to their rigorous protection.     

Assessing sensitivity of source term estimation

Source term estimation algorithms compute unknown atmospheric transport and dispersion modeling variables from concentration observations made by sensors in the field. Insufficient spatial and temporal resolution in the meteorological data as well as inherent uncertainty in the wind field data make source term estimation and the prediction of subsequent transport and dispersion extremely difficult. This work addresses the question: how many sensors are necessary in order to successfully estimate the source term and meteorological variables required for atmospheric transport and dispersion modeling?The source term estimation system presented here uses a robust optimization technique – a genetic algorithm (GA) – to find the combination of source location, source height, source strength, surface wind direction, surface wind speed, and time of release that produces a concentration field that best matches the sensor observations. The approach is validated using the Gaussian puff as the dispersion model in identical twin numerical experiments. The limits of the system are tested by incorporating additive and multiplicative noise into the synthetic data. The minimum requirements for data quantity and quality are determined by an extensive grid sensitivity analysis. Finally, a metric is developed for quantifying the minimum number of sensors necessary to accurately estimate the source term and to obtain the relevant wind information.     

Mercury contamination in alligators (Melanosuchus niger) from Mamirauá Reservoir (Brazilian Amazon) and human health risk assessment

Mercury (Hg) concentrations in muscles of wild alligators (Melanosuchus niger) from the Mamirauá Reservoir (a reference area in the Brazilian Amazon) and the human health risks associated with its consumption were assessed. The mean Hg concentration in alligator muscles was 0.407?±?0.114 μg/g (N?=?61). Close to 5 % of the muscle samples showed Hg levels above the World Health Organization guideline for fish consumption (0.5 μg/g). A positive and significant relationship was observed between Hg concentrations in muscle and the age of the specimens. The dose-response approach suggests that close to 27.4 years is required for half of the exposed specimens to attain 0.5 μg/g. The hazard quotient (HQ) is a risk indicator which defines the ratio of exposure level and a toxicological reference dose. HQ resulted above the unity for all the specimens when the ingestion rate for riverine communities (200 g of muscle per day) is considered, indicating the existence of hazard. When the ingestion rate for market consumers (28.57 g/day) is considered, the risks are much lower (mean HQ?=?0.55), suggesting that such group is not at risk. The establishment of local and regional ingestion rates for riverine populations and market consumers is extremely recommended.     

Effect of Watershed Subdivision and Filter Width on SWAT Simulation of a Coastal Plain Watershed1

Cho, Jaepil, Richard R. Lowrance, David D. Bosch, Timothy C. Strickland, Younggu Her, and George Vellidis, 2010. Effect of Watershed Subdivision and Filter Width on SWAT Simulation of a Coastal Plain Watershed. Journal of the American Water Resources Association (JAWRA) 46(3):586-602. DOI: 10.1111/j.1752-1688.2010.00436.x Abstract: The Soil and Water Assessment Tool (SWAT) does not fully simulate riparian buffers, but has a simple filter function that is responsive to filter strip width (FILTERW). The objectives of this study were to (1) evaluate SWAT hydrology and water quality response to changes in watershed subdivision levels and different FILTERW configurations and (2) provide guidance for selecting appropriate watershed subdivision for model runs that include the riparian buffer feature through the FILTERW parameter. Watershed subdivision level is controlled by the critical source area (CSA) which defines the minimum drainage area required to form the origin of a stream. SWAT was calibrated on a 15.7 km² subdrainage within the Little River Experimental Watershed, Georgia. The calibrated parameter set was applied to 32 watershed configurations consisting of four FILTERW representations for each of eight CSA levels. Streamflow predictions were stable regardless of watershed subdivision and FILTERW configuration. Predicted sediment and nutrient loads from upland areas decreased as CSA increased when spatial variations of riparian buffers are considered. Sediment and nutrient yield at the watershed outlet was responsive to different combinations of CSA and FILTERW depending on selected in-stream processes. CSA ranges which provide stable sediment and nutrient yields at the watershed outlet was suggested for avoiding significant modifications in selected parameter set.     

n-Hexane Biodegradation in Trickle Bed Air Biofilters

While hydrophilic compounds are degraded easily in Trickling bed air biofilters (TBABs), hydrophobic compounds are retarded until biological cultures produce a sufficient RNA or enzyme/protein to utilize this compound. Hydrophobic compounds are not readily bio-available which makes them reluctant to biodegradation as mass transfer between the gas and liquid phases is a rate limiting step. To enhance the destruction of hydrophobic compounds in TBABs, the utilization of surfactant was introduced to increase the solubility which helps overcoming the rate limiting step. The surfactant was used as well to limit the growth of excess biomass ensuring smooth flow through the biofilter bed and preventing short circuits. Two different non-ionic non-toxic surfactants were used in this study: Triton X-100 and Tomadol® 25-7. Two lab-scale controlled TBABs were operated for investigating the performance difference for n-Hexane as an example of hydrophobic volatile organic compound (VOC) with and without the addition of surfactant. Operating conditions in both TBABs were as follows: nutrient feed rate (2L/day), air flowrate (1.4L/min), bed depth (60cm), empty bed retention time (120s), bed material (diatomaceous earth pellets) and room-temperature. The inlet concentration was changed from 50 to 100ppmv. Acclimation period, removal profile along biofilter depth, nitrogen consumption, and CO₂ production were compared under continuous loading operation condition. The optimum concentration of surfactant in the nutrient feed was determined by a batch experiment. The effect of different surfactant concentrations on VOC water solubility with time was studied by considering different VOC concentration sets within the TBAB loading rate range.     

Bioremediation of atrazine-contaminated soil by forage grasses: transformation, uptake, and detoxification

A sound multi-species vegetation buffer design should incorporate the species that facilitate rapid degradation and sequestration of deposited herbicides in the buffer. A field lysimeter study with six different ground covers (bare ground, orchardgrass, tall fescue, timothy, smooth bromegrass, and switchgrass) was established to assess the bioremediation capacity of five forage species to enhance atrazine (ATR) dissipation in the environment via plant uptake and degradation and detoxification in the rhizosphere. Results suggested that the majority of the applied ATR remained in the soil and only a relatively small fraction of herbicide leached to leachates (<15%) or was taken up by plants (<4%). Biological degradation or chemical hydroxylation of soil ATR was enhanced by 20 to 45% in forage treatment compared with the control. Of the ATR residues remaining in soil, switchgrass degraded more than 80% to less toxic metabolites, with 47% of these residues converted to the less mobile hydroxylated metabolites 25 d after application. The strong correlation between the degradation of N-dealkylated ATR metabolites and the increased microbial biomass carbon in forage treatments suggested that enhanced biological degradation in the rhizosphere was facilitated by the forages. Hydroxylated ATR degradation products were the predominant ATR metabolites in the tissues of switchgrass and tall fescue. In contrast, the N-dealkylated metabolites were the major degradation products found in the other cool-season species. The difference in metabolite patterns between the warm- and cool-season species demonstrated their contrasting detoxification mechanisms, which also related to their tolerance to ATR exposure. Based on this study, switchgrass is recommended for use in riparian buffers designed to reduce ATR toxicity and mobility due to its high tolerance and strong degradation capacity.     

Surface applied water treatment residuals affect bioavailable phosphorus losses in Florida sands

Water treatment residuals (WTR) can reduce runoff P loss and surface co-application of P-sources and WTR is a practical way of land applying the residuals. In a rainfall simulation study, we evaluated the effects of surface co-applied P-sources and an Al-WTR on runoff and leacheate bioavailable P (BAP) losses from a Florida sand. Four P-sources, namely poultry manure, Boca Raton biosolids (high water-soluble P), Pompano biosolids (moderate water-soluble P), and triple super phosphate (TSP) were surface applied at 56 and 224kgPha(-1) (by weight) to represent low and high soil P loads typical of P- and N-based amendments rates. The treatments further received surface applied WTR at 0 or 10gWTRkg(-1) soil. BAP loss masses were greater in leachate (16.4-536mg) than in runoff (0.91-46mg), but were reduced in runoff and leachate by surface applied WTR. Masses of total BAP lost in the presence of surface applied WTR were less than approximately 75% of BAP losses in the absence of WTR. Total BAP losses from each of the organic sources applied at N-based rates were not greater than P loss from TSP applied at a P-based rate. The BAP loss at the N-based rate of moderate water-soluble P-source (Pompano biosolids) was not greater than BAP losses at the P-based rates of other organic sources tested. The hazards of excess P from applying organic P-sources at N-based rates are not greater than observed at P-based rates of mineral fertilizer. Results suggest that management of the environmental P hazards associated with N-based rates of organic materials in Florida sands is possible by either applying P-sources with WTR or using a moderate water-soluble P-source.