Quantifying MTBE biodegradation in the Vandenberg Air Force Base ethanol release study using stable carbon isotopes

Compound-specific isotope analysis (CSIA) was used to assess biodegradation of MTBE and TBA during an ethanol release study at Vandenberg Air Force Base. Two continuous side-by-side field releases were conducted within a preexisting MTBE plume to form two lanes. The first involved the continuous injection of site groundwater amended with benzene, toluene and o-xylene ("No ethanol lane"), while the other involved the continuous injection of site groundwater amended with benzene, toluene and o-xylene and ethanol ("With ethanol lane"). The delta(13)C of MTBE for all wells in the "No ethanol lane" remained constant during the experiment with a mean value of -31.3 +/- 0.5 per thousand (n=40), suggesting the absence of any substantial MTBE biodegradation in this lane. In contrast, substantial enrichment in (13)C of MTBE by 40.6 per thousand, was measured in the "With ethanol lane", consistent with the effects of biodegradation. A substantial amount of TBA (up to 1200 microg/L) was produced by the biodegradation of MTBE in the "With ethanol lane". The mean value of delta(13)C for TBA in groundwater samples in the "With ethanol lane" was -26.0 +/- 1.0 per thousand (n=32). Uniform delta(13)C TBA values through space and time in this lane suggest that substantial anaerobic biodegradation of TBA did not occur during the experiment. Using the reported range in isotopic enrichment factors for MTBE of -9.2 per thousand to -15.6 per thousand, and values of delta(13)C of MTBE in groundwater samples, MTBE first-order biodegradation rates in the "With ethanol lane" were 12.0 to 20.3 year(-1) (n=18). The isotope-derived rate constants are in good agreement with the previously published rate constant of 16.8 year(-1) calculated using contaminant mass-discharge for the "With ethanol lane".     

Enhancement of sorption capacity of cocoa shell biomass modified with non-thermal plasma for removal of both cationic and anionic dyes from aqueous solution

Environmental Science and Pollution Research - Removal of cationic dye, Azur II, and anionic dye, Reactive Red 2 (RR-2) from aqueous solutions, has been successfully achieved by using a modified...     

A Review of Known and Hypothetical Transmission Routes for Noroviruses

Human noroviruses (NoVs) are considered a worldwide leading cause of acute non-bacterial gastroenteritis. Due to a combination of prolonged shedding of high virus levels in feces, virus particle shedding during asymptomatic infections, and a high environmental persistence, NoVs are easily transmitted pathogens. Norovirus (NoV) outbreaks have often been reported and tend to affect a lot of people. NoV is spread via feces and vomit, but this NoV spread can occur through several transmission routes. While person-to-person transmission is without a doubt the dominant transmission route, human infective NoV outbreaks are often initiated by contaminated food or water. Zoonotic transmission of NoV has been investigated, but has thus far not been demonstrated. The presented review aims to give an overview of these NoV transmission routes. Regarding NoV person-to-person transmission, the NoV GII.4 genotype is discussed in the current review as it has been very successful for several decades but reasons for its success have only recently been suggested. Both pre-harvest and post-harvest contamination of food products can lead to NoV food borne illness. Pre-harvest contamination of food products mainly occurs via contact with polluted irrigation water in case of fresh produce or with contaminated harvesting water in case of bivalve molluscan shellfish. On the other hand, an infected food handler is considered as a major cause of post-harvest contamination of food products. Both transmission routes are reviewed by a summary of described NoV food borne outbreaks between 2000 and 2010. A third NoV transmission route occurs via water and the spread of NoV via river water, ground water, and surface water is reviewed. Finally, although zoonotic transmission remains hypothetical, a summary on the bovine and porcine NoV presence observed in animals is given and the presence of human infective NoV in animals is discussed.     

Kinetics of the reactions of soot surface-bound polycyclic aromatic hydrocarbons with the OH radicals

The kinetics of the heterogeneous reaction of OH radicals with 15 polycyclic aromatic hydrocarbons (PAHs) present in laboratory generated simulated kerosene combustion soot was studied at T = 290 K in a low pressure discharge-flow reactor combined with an electron-impact mass spectrometer. The kinetics of soot-bound PAH consumption in reaction with OH were monitored using off-line HPLC measurements of their concentrations in soot samples as a function of time of exposure to OH. Concentration of OH radicals in the gas phase was measured by mass spectrometry. The first-order rate constants measured for the individual PAH at T = 290 K ranged from 0.02 to 0.04 s^?1 and were found to be independent of the OH concentration ([OH] = (0.34–2.5) × 10¹² molecule cm^?3) and of the molecular structure of the PAH. In addition, the uptake coefficient of OH on soot surface and the diffusion coefficient of OH in He were measured to be 0.19 ± 0.03 (calculated with geometric surface area) and (615 ± 80) Torr cm² s^?1, respectively. Comparison of the results on the PAH + OH reaction to those from previous studies carried out on different carbonaceous substrates, indicates probable dependence of the heterogeneous reactivity of PAH toward OH on the substrate nature. Rapid reaction with OH can be an important potential pathway of the atmospheric degradation of non-volatile PAH present mainly in the particulate phase in the atmosphere.     

Incentive mechanisms to enforce sustainable forest exploitation

Using a two-player differential game approach, this paper deals with the issue of tropical deforestation. The assumption is that developing forestry countries (called the South) do not, or cannot, necessarily have the same utility for forest conservation than the developed countries (called the North). Given the global nature of the problem, we assume that it may be wise that the North offers financial support to the South to help in achieving a sustainable forest policy. We derive sustainable and short-run deforestation policies and provide a transfer mechanism which insures that the South will indeed implement the sustainable one.     

Chemical recognition of queen cells by honey bee workersApis mellifera (Hymenoptera: Apidae)

Summary Honey bee workers are able to nurse or to destroy and thus to recognize the capped queen cells containing a pupa. Fatty acid esters, especially methyl oleate, methyl palmitate and ethyl oleate were found in significant amounts on the queen pupal cuticle. Methyl oleate, the major component, along with smaller amounts of methyl linoleate and methyl linolenate, were involved in the recognition of queen cells by workers. In natural conditions of the colony, queen cells containing a paraffin pupal lure with methyl oleate were accepted 5.9 days by workers, releasing about 1.8 queen pupa equivalents during that period, when control cells (without ester) were kept only 2.1 days. Although these esters are non specific to honey bees, they are of great importance in social regulation of the honey bee colony.     

Mechanized Logging, Market Hunting, and a Bank Loan in Congo

Financing for logging of tropical moist forests in the Republic of Congo is commonly sought in the form of loans from multilateral development banks. Pressure from nongovernmental conservation organizations and from within the banks themselves has resulted in their beginning to assess the environmental consequences of such loans. We conducted one of the first such assessments of an African Development Bank loan to a logging company. Geographic isolation, resulting transportation costs, and market demands have forced commercial loggers within the Sangha region of Congo to exploit only the most valuable timber. This form of timber extraction destroys an average of 6.8% of the canopy and thus, unlike clear cutting, was expected to have a minimal impact on wildlife populations. Line transect counts showed, however, that primate abundance was exceedingly low in logged forest. We believe this is not a direct consequence of canopy reduction, but results from the extremely intensive market hunting that coincides with timber surveying and extraction. Weapons and hunting camps were common, and logging company vehicles transported primates, duikers and other game daily. Wildlife laws of Congo are openly violated and they are not enforced. While market hunting is clearly facilitated and intensified by the presence of logging concessions, it is the Congo's highly urbanized population that provides the ever growing demand for meat, a demand not being met through animal husbandry. Thus, although selective logging in the absence of hunting may have only limited adverse effects on wildlife, when the two are combined the consequences are grave for the Sangha region's wildlife. Loans to logging companies from the African Development Bank should incorporate conditions for ensuring wildlife conservation.     

Maintenance of variable responses for coping with wetland drying in freshwater turtles

Aquatic animals inhabiting temporary wetlands must respond to habitat drying either by estivating or moving to other wetlands. Using radiotelemetry and capture mark recapture, we examined factors influencing the decisions made by individuals in a population of freshwater turtles (Chelodina longicollis) in response to wetland drying in southeastern Australia. Turtles exhibited both behaviors, either remaining quiescent in terrestrial habitats for variable lengths of time (terrestrial estivation) or moving to other wetlands. Both the proportion of individuals that estivated terrestrially and the time individuals spent in terrestrial habitats increased with decreasing wetland hydroperiod and increasing distance to the nearest permanent wetland, suggesting behavioral decisions are conditional or state dependent (i.e., plastic) and influenced by local and landscape factors. Variation in the strategy or tactic chosen also increased with increasing isolation from other wetlands, suggesting that individuals differentially weigh the costs and benefits of residing terrestrially vs. those of long-distance movement; movement to other wetlands was the near universal strategy chosen when only a short distance must be traveled to permanent wetlands. The quality of temporary wetlands relative to permanent wetlands at our study site varies considerably and unpredictably with annual rainfall and with it the cost-benefit ratio of each strategy or tactic. Residency in or near temporary wetlands is more successful during wet periods due to production benefits, but movement to permanent wetlands is more successful, or least costly, during dry periods due to survival and body condition benefits. This shifting balance may maintain diversity in response of turtles to the spatial and temporal pattern in wetland quality if their response is in part genetically determined.     

Measurement of metal bioaccessibility in vegetables to improve human exposure assessments: field study of soil–plant–atmosphere transfers in urban areas,South China

The quality of cultivated consumed vegetables in relation to environmental pollution is a crucial issue for urban and peri-urban areas, which host the majority of people at the global scale. In order to evaluate the fate of metals in urban soil–plant–atmosphere systems and their consequences on human exposure, a field study was conducted at two different sites near a waste incinerator (site A) and a highway (site B). Metal concentrations were measured in the soil, settled atmospheric particulate matter (PM) and vegetables. A risk assessment was performed using both total and bioaccessible metal concentrations in vegetables. Total metal concentrations in PM were (mg kg^?1): (site A) 417 Cr, 354 Cu, 931 Zn, 6.3 Cd and 168 Pb; (site B) 145 Cr, 444 Cu, 3289 Zn, 2.9 Cd and 396 Pb. Several total soil Cd and Pb concentrations exceeded China’s Environmental Quality Standards. At both sites, there was significant metal enrichment from the atmosphere to the leafy vegetables (correlation between Pb concentrations in PM and leaves: r = 0.52, p < 0.05) which depended on the plant species. Total Cr, Cd and Pb concentrations in vegetables were therefore above or just under the maximum limit levels for foodstuffs according to Chinese and European Commission regulations. High metal bioaccessibility in the vegetables (60–79 %, with maximum value for Cd) was also observed. The bioaccessible hazard index was only above 1 for site B, due to moderate Pb and Cd pollution from the highway. In contrast, site A was considered as relatively safe for urban agriculture.