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121.
P. R. N. Vieira-Girão C. B. Falcão I. R. C. B. Rocha H. M. R. Lucena F. H. F. Costa G. Rádis-Baptista 《Food and environmental virology》2017,9(3):277-286
The shrimp farming has been converted into a mature aquaculture industry dealing with over millions of metric tonnes of processed commodities. Nevertheless, the global shrimp productions are constantly threatened by disease outbreaks, mainly triggered by rapidly disseminating viruses. Infectious myonecrosis virus (IMNV) is one of these epizootic agents affecting shrimp production in Brazil, of which no treatment exists. Herein, the antiviral activity against IMNV of an eicosapeptide, named Ctn[15-34], derived from a member of the cathelicidin family of antimicrobial peptides, was demonstrated. Cultures of hemocytes from Litopenaeus vannamei were established that support IMNV replication and infectivity titration. The cytotoxic effect of IMNV in culture and the in vitro anti-IMNV activity of Ctn[15-34] were assessed using a high-sensitive fluorescent-based method in combination with quantitative PCR. The Ctn[15-34] (<12.5 µM) neutralized the toxic effects of IMNV at loads sufficient to kill 50% of shrimp hemocytes. This study reported for the first time the replication of IMNV in vitro and the employment of a straightforward methodology to assess cell viability and viral/antiviral activities. In addition, it provided the basis for the development of the anti-infective multi-effector Ctn[15-34] eicosapeptide and analogs as components of antiviral formulations against shrimp viral diseases. 相似文献
122.
Dr Gloria Pelizzo Giancarlo Conoscenti Karim D. Kalache Francesco Vesce Paolo Guerrini Luigi Cavazzini 《黑龙江环境通报》2003,23(4):292-294
An Erratum has been published for this article in Prenatal Diagnosis 23(9) 2003, 771 Antenatal detection of an isolated abdominal cyst was found to be a pancreatoblastoma in a female fetus with Beckwith–Wiedemann syndrome. Prenatal and post-natal features and management of this very rare tumour are discussed. Molecular investigation disclosed a mosaic paternal 11p15 uniparental disomy in the tumoral cells. The prognosis of a congenital pancreatoblastoma is good if complete surgical excision is achieved. However, the association with Beckwith–Wiedemann syndrome requires a prolonged follow-up because of the increased risk of developing malignant tumours. Copyright © 2003 John Wiley & Sons, Ltd. 相似文献
123.
124.
We present a family who sought prenatal diagnosis in order to bear a healthy child to serve as an HLA–identical bone marrow donor for their son affected with Wiskott–Aldrich syndrome. They intended to abort HLA-incompatible fetuses who would have been unsuitable bone marrow donors. This case led us to conclude that prenatal diagnosis should not be used to benefit a third party or facilitate the conception or abortion of a fetus for the purpose of generating an organ for transplantation. The limits of parental autonomy and physician responsibility are discussed. 相似文献
125.
Marcel Clusa Carlos Carreras Marta Pascual Stephen J. Gaughran Susanna Piovano Cristina Giacoma Gloria Fernández Yaniv Levy Jesús Tomás Juan Antonio Raga Fulvio Maffucci Sandra Hochscheid Alex Aguilar Luis Cardona 《Marine Biology》2014,161(3):509-519
Loggerhead turtles nesting in the Mediterranean Sea exhibit remarkable genetic structuring. This paper tests the hypothesis that young loggerhead turtles from different rookeries do not distribute homogeneously among the major Mediterranean foraging grounds, due to a complex pattern of surface currents. We extracted long fragments of mitochondrial DNA from 275 stranded or bycaught juvenile turtles from six foraging grounds (Catalano-Balearic Sea, Algerian basin, Tyrrhenian Sea, Adriatic Sea, northern Ionian Sea and southern Levantine Sea). We used a Bayesian mixed-stock analysis to estimate the contributions from rookeries in the Mediterranean, the North-west Atlantic and Cape Verde to the studied foraging grounds. Differences were found in the relative contribution of juvenile turtles of Atlantic and Mediterranean origin to each foraging ground. A decreasing proportion of Atlantic juveniles was detected along the main surface current entering the Mediterranean, with a high prevalence of turtles from eastern Florida in the Algerian basin and lower numbers elsewhere. In regard to the turtles of Mediterranean origin, juveniles from Libya prevailed in central and western Mediterranean foraging grounds other than the Algerian basin. Conversely, the Adriatic Sea was characterised by a large presence of individuals from western Greece, while the southern Levantine Sea was inhabited by a heterogeneous mix of turtles from the eastern Mediterranean rookeries (Turkey, Lebanon and Israel). Overall, the distribution of juveniles may be related to surface circulation patterns in the Mediterranean and suggests that fisheries might have differential effects on each population depending on the overlap degree between foraging and fishing grounds. 相似文献
126.
Remote sensing of aquatic vegetation: theory and applications 总被引:2,自引:0,他引:2
Aquatic vegetation is an important component of wetland and coastal ecosystems, playing a key role in the ecological functions
of these environments. Surveys of macrophyte communities are commonly hindered by logistic problems, and remote sensing represents
a powerful alternative, allowing comprehensive assessment and monitoring. Also, many vegetation characteristics can be estimated
from reflectance measurements, such as species composition, vegetation structure, biomass, and plant physiological parameters.
However, proper use of these methods requires an understanding of the physical processes behind the interaction between electromagnetic
radiation and vegetation, and remote sensing of aquatic plants have some particular difficulties that have to be properly
addressed in order to obtain successful results. The present paper reviews the theoretical background and possible applications
of remote sensing techniques to the study of aquatic vegetation. 相似文献
127.
da Costa Machado Matos Carvalho IM Cavalcante AA Dantas AF Pereira DL Rocha FC Oliveira FM Da Silva J 《Chemosphere》2011,82(7):1056-1061
Sodium metabisulfite is used in marine shrimp harvesting to prevent the occurrence of black spots. Shrimps are soaked in a sodium metabisulfite solution in ice, which is disposed of in sewages that run into marine canals, creating an environmental hazard. This study evaluates the toxicity and mutagenicity caused by sodium metabisulfite in sea waters and sediments collected in a shrimp farm in Cajueiro da Praia (Luis Correia), state of Piauí, Brazil, using the Allium cepa assay. Water and sediment samples were collected in the dry and in the rainy seasons, in three sites: upstream the shrimp farm (Site 1), at the point sodium metabisulfite is discharged (Site 2), and 100 m downstream the farm (Site 3). Three sample dilutions were used (50%, 25% and 10%) for all samples. A negative control (well water) and a positive control (copper sulfate 0.0006 mg mL−1) were used in each experiment. At the end of the 72-h exposure period, onion roots were measured and removed. Mutagenicity analysis included the determination of mitotic index, chromosomal aberrations and the detection of micronuclei; analysis of root size and mitotic index were used as an index of toxicity. The A. cepa assay revealed that the water and sediments samples collected in the Piauí coast contaminated with sodium metabisulfite induce toxicity. The results demonstrate that the assay may be used as a regular tool in the analysis of water parameters in shrimp farms in the coast of Piauí state, and in strategies to preserve the region’s marine ecosystem. 相似文献
128.
129.
Carlos Costa Javier Rodríguez M. Carmen Márquez 《Environmental Modeling and Assessment》2009,14(6):739-747
A simplified dynamic model for the activated sludge process with high-strength wastewaters is presented. The model is based
on activated sludge models and predicts effluent chemical oxygen demand concentration using only four parameters. Experimental
data for testing the model were obtained in an activated sludge plant using flax retting wastewater. The proposal of the model
is described: mass balance to the system, selection of variables and behaviour of the theoretical values versus experimental
results. 相似文献
130.
Ramsdorf WA Ferraro MV Oliveira-Ribeiro CA Costa JR Cestari MM 《Environmental monitoring and assessment》2009,158(1-4):77-85
Different genetic biomarkers have been used to evaluate the pollution effects of mutagenic agents such as metals and also a great variety of chemicals delivered on the environment by human activities. This way, the aim of the present report was to evaluate the effects of inorganic lead in fishes through the frequency of piscine micronuclei and nuclear morphological alterations in peripheral cells, chromosomal aberration frequency and comet assays in blood and kidney cells. Specimens of Hoplias malabaricus received different doses of lead by intra-peritoneal injections at time of 96 h. There was not a significant difference between control and treated groups for the piscine micronucleus and chromosomal aberration assays. In the comet assays there was a significant difference between control and contaminated groups. However, a significant difference between the applied doses was not observed. The results obtained with the comet assays also show that blood presented a higher sensibility than the kidney tissue, possibly due to the acute contamination. Although the results showed the genotoxic potential of lead at the 21 and 63 microg Pb(2 + )/g doses for both tissues, the lowest dose is considered more appropriate for future bioassays. 相似文献