Rival recognition affects male contest behavior in sand lizards (Lacerta agilis)

Individual male sand lizards meet repeatedly during mate search and engage in costly interactions. If males can recognize rivals, the number of costly fights with a predictable outcome may be reduced. In staged contests between males, second interactions are on average significantly shorter than first ones, suggesting individual recognition. When aggressive behavior differs substantially between the sexes, the more aggressive sex may be predicted to have more variation in skin traits located on the parts of the body displayed during contests; this would facilitate individual recognition. Male (aggressive) sand lizards are more variable in two of three lateral skin traits displayed during males' contests, while females (non-aggressive) are more variable in one dorsal skin trait; in four other dorsal traits there was no difference in variability between the sexes.     

Space use in lions, <Emphasis Type="Italic">Panthera leo</Emphasis>, in the Selous Game Reserve: social and ecological factors

Habitat qualities, such as food supply or access to refuges, often influence home-range size. Furthermore, such qualitative differences usually lead to conspecific competition over space, which can be an important factor in determining the distribution of individuals within populations. In carnivores, patterns of resource dispersion are hypothesized to determine home range-size and group size. But in contests over space (or other resources), larger groups usually dominate smaller ones, and group size should therefore also affect home-range size. Here I describe the space use of lions, Panthera leo, in the Selous Game Reserve, Tanzania, and ask whether space use is related to pride size, habitat, or relatedness. Home ranges varied in size, but size showed no correlation to number of adult females in the pride or to habitat type. Lions exhibited a significant preference for riverine and short-grass habitat, and a significant avoidance of acacia woodland. Habitat preference ratios largely reflected prey availability in each habitat. Outer areas, as well as core areas of home ranges, were often used by two or more prides. Overlaps showed no correlation to relatedness among prides or habitat type. Thus, whereas home-range sizes and overlaps were determined by factors that could not be revealed by demographic factors or analyses of habitat composition or genetic structure, lion space use within each home range seemed driven mostly by prey availability, which mainly varies with habitat type.     

Screening life cycle assessment (LCA) of tomato ketchup: a case study

A screening life cycle assessment (LCA) of tomato ketchup has been carried out. The purpose was to identify ‘hot-spots', that is parts of the life-cycle that are important to the total environmental impact. The system investigated includes agricultural production, industrial refining, packaging, transportation, consumption and waste management. Energy use and emissions were quantified and some of the potential environmental effects assessed. Packaging and food processing were found to be hot-spots for many, but not all, of the impact categories investigated. For primary energy use, the storage time in a refrigerator (household phase) was found to be a critical parameter.     

An erosion-based land classification system for military installations

The universal soil loss equation (USLE) has been integrated with a geographic information system known as the geographical resources analysis support system (GRASS) to create a land classification system for use by military trainers and land managers to minimize the environmental impacts of military training activities. The USLE provides an estimate of current average annual sheet and rill erosion based upon factors representing climate, soil erodibility, topography, cover, and conservation support practices. The erosion estimate is compared to erosion tolerance values to produce an expression of the current erosion status. An index of inherent site erodibility is also achieved through manipulation of the USLE. Based on published soil surveys, satellite imagery, and ground-truth vegetation transects, data layers are created within GRASS for each of the component factors of the USLE. Appropriate mathematical operations are performed with the data layers, and color-coded maps are produced that represent the erosion status and erodibility index for each 50-m × 50-m area of soil surface. These maps aid military trainers and land managers in scheduling appropriate kinds and intensities of military training activities.     

Chemosensory mate recognition may facilitate prolonged mate guarding by male snow skinks, Niveoscincus microlepidotus

We provide evidence that male lizards can use chemosensory cues to identify individual females and probably therefore maintain long-term associations with these females in the wild. In the laboratory, males preferentially followed the scent trail of their vitellogenic female “partner” rather than that of another vitellogenic female. Our 5-year field study of the small viviparous scincid lizard (Niveoscincus microlepidotus) in alpine Tasmania showed that sexually mature males and females commonly formed “pairs” for long periods (on average 29 days). These pairs occurred primarily during the mating season, always involved one adult male and one adult female, and usually involved vitellogenic rather than gravid females. Our laboratory experiments suggest that a significant factor in maintaining those prolonged partnerships is male scent trailing of partners. Received: 28 October 1997 / Accepted after revision: 28 May 1998     

Workshop report on the extraction of foetal DNA from maternal plasma

Objective Cell free foetal DNA (cff DNA) extracted from maternal plasma is now recognized as a potential source for prenatal diagnosis but the methodology is currently not well standardized. To evaluate different manual and automated DNA extraction methods with a view to developing standards, an International Workshop was performed. Methods Three plasma pools from RhD-negative pregnant women, a DNA standard, real-time-PCR protocol, primers and probes for RHD were sent to 12 laboratories and also to one company (Qiagen, Hilden, Germany). In pre-tests, pool 3 showed a low cff DNA concentration, pool 1 showed a higher concentration and pool 2 an intermediate concentration. Results The QIAamp DSP Virus Kit, the High Pure PCR Template Preparation Kit, an in-house protocol using the QIAamp DNA Blood Mini Kit, the CST genomic DNA purification kit, the Magna Pure LC, the MDx, the M48, the EZ1 and an in-house protocol using magnetic beads for manual and automated extraction were the methods that were able to reliably detect foetal RHD. The best results were obtained with the QIAamp DSP Virus Kit. The QIAamp DNA Blood Mini Kit showed very comparable results in laboratories that followed the manufacturer's protocol and started with ≥ 500 µL plasma. One participant using the QIAamp DNA Blood Midi Kit failed to detect reliably RHD in pool 3. Conclusions This workshop initiated a standardization process for extraction of cff DNA in maternal plasma. The highest yield was obtained by the QIAamp DSP Virus Kit, a result that will be evaluated in more detail in future studies. Copyright © 2007 John Wiley & Sons, Ltd.     

The importance of atmospheric deposition, charge and atomic mass to the dynamics of minor and rare elements in developing, ageing, and wilted leaves of beech (Fagus sylvatica L.)

The amounts of sixty elements in developing, maturing, senescent and wilting leaves, and in the wintering dead leaves attached to the branches, are reported for a beech (Fagus sylvatica) forest on mor Podzol in south Sweden, a site with no local sources of pollution or geological anomalies. The amounts (contents per leaf) of K (potassium), Rb (rubidium), Cs (caesium), Cu (copper) and P (phosphorus) were highest in young leaves, decreasing throughout the growing season and usually in the subsequent winter. The entirely opposite pattern with a continuous, mostly even increase of the amounts was measured with Be (beryllium), Ba (barium), Hg (mercury), Al (aluminium), Tl (thallium), Pb (lead), Bi (bismuth), V (vanadium), W (tungsten), As (arsenic), Sb (antimony), and Se (selenium). Amounts of rare-earth elements and some transition metals, such as Co (cobalt), Ti (titanium), and the actinides Th (thorium) and U (uranium) were more stable during the growing season, after an initial increase in early summer, but increased greatly in the winter. This winter increase in dead attached leaves has to be accounted for by uptake from long-distance transported constituents in dry and wet deposition. It was similar to deposition rate estimates using moss carpets from the same locality. A passive uptake was positively related to ionic charge and atomic mass. However, the amounts of several, mainly non-essential elements, such as Ni (nickel), Sc (scandium), Zr (zirconium), Cr (chromium), Ag (silver), and Cd (cadmium) were not much lower in the young or maturing leaves than in the wintered dead leaves of this deciduous (hardwood) forest and a proportion apparently originated from internal translocation in the trees. Seasonal fluxes or cycling of many of the scarce or rare elements reported here have never been studied before in forest ecosystems.     

The role of DDE,PCB, coplanar PCB and eggshell parameters for reproduction in the white-tailed sea eagle (Haliaeetus albicilla) in Sweden

The reproduction of white-tailed sea eagles was monitored in 1964-1999 in 3 differently contaminated sub-populations: Baltic Sea coast (Bp), inland central Sweden (Ip) and Lapland (Lp). 249 dead eggs from 205 clutches were obtained for analyses of DDE and PCBs and for eggshell measurements. A desiccation index (Di) value was calculated for each egg as a measure of water loss through the shell. In the highly contaminated Bp, p,p'-DDE concentrations in the eggs decreased continuously and 5-fold during the study period and PCB concentrations decreased 3-fold from the mid 1980s. The PCB pattern changed slightly over time towards more high-chlorinated congeners but the relative toxicity of the PCB mixture, expressed as 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents (TEQ), remained constant and TEQ can be assumed to have decreased in a similar way as PCB over time. Productivity (P), shell thickness (St), shell index (Si) and Di increased over time in the Bp but no change in Di or productivity occurred in the Lp, where residue concentrations were 5-8 times lower. P of the Bp was not correlated to St or Si but was negatively correlated to Di, DDE and PCB. An S-shaped dose-response relationship was indicated between P and DDE. After 1988, when the PCB/DDE ratio was considerably higher than previously, PCB but not DDE concentrations were significantly higher in eggs with dead embryos as compared to undeveloped eggs, implying lethal concentrations of PCB, and a LOEL of 320 pg g-1 TEQ is suggested for embryo mortality. In a subset of 21 eggs, representing productive and unproductive females, analyzed for a selection of coplanar PCB congeners, tris(4-chlorophenyl) methanol and bis(4-chlorophenyl) sulphone, there was no evidence for a correlation between P and any of these compounds. A reduction in residue concentrations in old females did not lead to increased P or improved Di-values, indicating a remaining effect from a previous, higher exposure to contaminants. The inability to reproduce included a high rate of undeveloped eggs, indicating effects at a prezygotic stage. P showed the strongest correlation with Di, and Di was most strongly correlated to DDE. Thus, the remaining effect of previous exposure resulted in a stronger correlation to the symptom (Di) rather than to the suggested causative agent (DDE). LOEL values for depressed P were estimated at 120 micrograms g-1 DDE and 500 micrograms g-1 PCB (lipid basis). It is concluded that the major reason for depressed P during the study period was DDE, but that effects also from PCB were largely concealed by the effects from DDE.     

生产体系对肉产品(主要是猪肉)品质的影响

肉产品的技术、营养和感官品质在屠宰前后可能会受到多种交互作用因素的影响.从文献来看,以创造猪肉可持续生产体系为目标的各种变革,通常对肉的最终pH值没有影响或影响较小.一些研究表明,可持续生产体系降低了猪肉的持水能力.与常规饲养体系相比,户外生产被认为更能增加猪肉的剪切力值.肉色的影响因素很多,这些因素可能导致猪肉颜色更暗、色素含量增加,或猪肉颜色更浅淡、结构有所变化.在使用含有多不饱和脂肪酸饲料的生产体系中,肉产品所含类脂的不饱和性更强,肉产品的营养价值更高.一句话,必须考虑各因素间的组合效应如:饲养、生产体系、遗传型、性别和宰前处理等对肉品质量的影响.     

The influence of production systems on meat quality, with emphasis on pork

Technological, nutritional, and sensory meat quality may be influenced by multiple interacting factors before and after slaughter. Reviewing the literature, it can be seen that alterations aimed at creating more sustainable production systems for pig meat generally have either no effect or a lowering effect on the ultimate pH value of the meat. Some studies indicate reduced water-holding capacity in sustainably produced pork. Outdoor production may be expected to increase further the shear force of the meat compared to conventional systems. The color may be affected in different ways, leading to either darker, more pigmented meat or paler, structurally effected meat. The more unsaturated profile of the lipids in meat produced in a system that includes feeds containing polyunsaturated fatty acids is favorable with respect to the nutritional quality of the meat. To conclude, the combinatory effects of, for example, feeding, production systems, genotype, sex, and preslaughter treatments, have to be addressed.