Genotoxic potential of selected cytostatic drugs in human and zebrafish cells

Due to their increasing use, the residues of anti-neoplastic drugs have become emerging pollutants in aquatic environments. Most of them directly or indirectly interfere with the cell’s genome, which classifies them into a group of particularly dangerous compounds. The aim of the present study was to conduct a comparative in vitro toxicological characterisation of three commonly used cytostatics with different mechanisms of action (5-fluorouracil [5-FU], cisplatin [CDDP] and etoposide [ET]) towards zebrafish liver (ZFL) cell line, human hepatoma (HepG2) cells and human peripheral blood lymphocytes (HPBLs). Cytotoxicity was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and acridine orange/ethidium bromide staining. All three drugs induced time- and dose-dependent decreases in cell viability. The sensitivity of ZFL and HepG2 cells towards the cytotoxicity of 5-FU was comparable (half maximal inhibitory concentration (IC₅₀) 5.3 to 10.4 μg/mL). ZFL cells were more sensitive towards ET- (IC₅₀ 0.4 μg/mL) and HepG2 towards CDDP- (IC₅₀ 1.4 μg/mL) induced cytotoxicity. Genotoxicity was determined by comet assay and cytokinesis block micronucleus (CBMN) assay. ZFL cells were the most sensitive, and HPBLs were the least sensitive. In ZFL cells, induction of DNA strand breaks was a more sensitive genotoxicity endpoint than micronuclei (MNi) induction; the lowest effective concentration (LOEC) for DNA strand break induction was 0.001 μg/mL for ET, 0.01 μg/mL for 5-FU and 0.1 μg/mL for CDDP. In HepG2 cells, MNi induction was a more sensitive genotoxicity endpoint. The LOEC values were 0.01 μg/mL for ET, 0.1 μg/mL for 5-FU and 1 μg/mL for CDDP. The higher sensitivity of ZFL cells to cytostatic drugs raises the question of the impact of such compounds in aquatic ecosystem. Since little is known on the effect of such drugs on aquatic organisms, our results demonstrate that ZFL cells provide a relevant and sensitive tool to screen genotoxic potential of environmental pollutant in the frame of hazard assessment.     

Alteration of cholinesterase activity as possible mechanism of silver nanoparticle toxicity

Due to their broad-spectrum antimicrobial activity, silver nanoparticles (AgNPs) have been used in a large number of commercial and medical products. Such proliferated AgNP production poses toxicological and environmental issues which need to be addressed. The present study aimed to investigate the effects of AgNPs on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), important enzymes in areas of neurobiology, toxicology and pharmacology. Three different AgNPs, prepared by the chemical reduction using trisodium citrate, hydroxylamine hydrochloride (Cl-AgNPs), and borohydride following stabilization with poly(vinyl alcohol), were purified and characterised with respect to their sizes, shapes and optical properties. Their inhibition potential on AChE and BChE was evaluated in vitro using an enzyme assay with o-nitrophenyl acetate or o-nitrophenyl butyrate as substrates, respectively. All three studied AgNPs were reversible inhibitors of ChEs. Among tested nanoparticles, Cl-AgNP was found to be the most potent inhibitor of both AChE and BChE. Although the detailed mechanism by which the AgNPs inhibit esterase activities remains unknown, structural perturbation of the enzyme may be the common mode of ChE inhibition by AgNPs.     

Declining coastal piscivore populations in the Baltic Sea: Where and when do sticklebacks matter?

Intraguild predation interactions make fish communities prone to exhibit alternative stable states with either piscivore or prey fish dominance. In the Baltic Sea, local declines of coastal piscivores like perch (Perca fluviatilis) have been observed to coincide with high densities of sticklebacks (Gasterosteus aculeatus). Mechanisms behind this shift between piscivore and stickleback dominance were studied both experimentally and in field. Results showed that predation by sticklebacks has a strong negative effect on perch larvae survival, but this effect rapidly decreases with increasing perch size, likely due to gape limitations and digestion constraints in sticklebacks. Large spatial and temporal variations in patterns of stickleback migration into perch spawning sites were observed. Whether or not high density of sticklebacks will cause declines in coastal piscivore populations is suggested to depend on the availability of spawning sites in which sticklebacks do not migrate into or arrive late in the reproduction season of coastal piscivores.     

Opportunities and Challenges to Capturing the Multiple Potential Benefits of REDD+ in a Traditional Transnational Savanna-Woodland Region in West Africa

The REDD+ scheme of the United Nations intends to offer developing countries financial incentives to reduce the rates of deforestation and forest degradation for reducing global CO₂ emissions. This is combined with building carbon stocks in existing wooded ecosystems and fostering other soil, biodiversity and water conservation objectives. Successful application of REDD+ to the Xylophone Triangle of West Africa faces substantial challenges and risks to both meeting REDD+ objectives and to the local people’s rights and livelihoods. The transnationality of the culturally coherent area requires collaboration of three national governments. The opportunities, however, are great to capitalize on the region’s biodiversity, the well-developed traditional ecological knowledge and the use of local medicinal plants as an integral part of the agro-ecosystem. Possibilities open to, not only sequester carbon, but also to increase the resilience of the ecosystem and of independent rural livelihoods in the face of climate change and globalization.     

Levels and possible sources of PCDD/PCDF in sediment and pike samples from Swedish lakes and rivers. (Part one)

One goal of the Swedish survey project is to discover possible sources of PCDD/F. Bottom sediment and pike were sampled at various sites near different types of industries along the coast line of two lakes and one river. The results show background levels of PCDD/F in some lakes which can only be explained by atmospheric input. In some lakes paper mill emissions of chlorinated PCDD/F give high levels of local contamination (≈1 ng/g IG) within short distances (<1 km) from the source. PCDD/F from paper mill effluents are transported long distances and traces can be detected over the entire lake area. The PCDD/F levels in pike are correlated with levels in the underlying sediment.     

在瑞典白尾海雕(Haliaeetus albicilla)繁殖过程中DDE、PCB、共面PCB和蛋壳参数所起的作用

1964～1999年,对三个受不同污染的瑞典白尾海雕亚种群[波罗的海沿岸种群(Bp)、瑞典中部内陆种群(Ip)和拉普兰种群(Lp)]的繁殖进行了监测.     

运用卫星数据和来自国家森林清查的野外数据进行全国森林变量估计

瑞典约有55%的土地面积被森林所覆盖.目前,瑞典的森林覆盖没有统一的和地理学上明晰的描述.现存的独立性数据源是Landsat TM和SPOT HRV卫星数据、地图蒙片(map mask)和森林清查样地.它们在一起,使森林变量(如材枳和林分年龄)的计算机估计成为可能.这些估计的精确度在像素水平上是低的,但在林分水平上较高.在规划木材资源应如何加以利用或出于监测目的时,这类基于栅格的森林数据对政府来说是有帮助的.本文描述了以有效成本建立森林变量估计全国数据库的生成流程.我们在林分水平上评价了瑞典西南部某地森林变量估计的精确度.结果表明,总材积估计的整体均方根误差为33%,年龄估计的整体均方根误差为23%.     

Does Tephrosia candida as fallow species, hedgerow or mulch improve nutrient cycling and prevent nutrient losses by erosion on slopes in northern Viet Nam?

Agroforestry is considered to be a promising alternative to short-fallow shifting cultivation or other monocropping systems. An on-farm experiment was established in 1996 in northern Viet Nam to examine the contribution of the leguminous bush Tephrosia candida (Roxb.) D.C. as a fallow or hedgerow species and as a mulch producer to improve nutrient cycling and prevent nutrient losses by erosion. The systems tested were upland rice monocropping (Mono), natural fallow (NaFa), fallow of Tephrosia (TepFa), hedgerow intercropping with upland rice (Oryza sativa L.) and internal mulching using pruned Tephrosia biomass (TepAl), and upland rice with external mulching using Tephrosia biomass (TepMu). Over two cropping seasons, from April 1996 to April 1998, nutrients recycled and inputs and exports were recorded, as well as changes in C-, N- and P-pools, and in pH in the 0–5 cm topsoil layer.The Tephrosia systems (TepFa, TepAl, TepMu) prevented nutrient losses by erosion effectively. Compared to the NaFa system, the TepFa system accumulated 34% more N in the above-ground plant parts and increased topsoil N by 20%, probably due to N-fixation. There was a trend that the less labile P-pools (NaOH-P) were reallocated into the more labile P-pools (Bicarb-P) in the soil of the TepFa system. Burning released significant amounts of the inorganic P-pools in both the NaFa and TepFa systems and this effect seemed to be more pronounced in the TepFa than in the NaFa. Organic input to crop export ratios for N and P were >1 in the TepAl and TepMu treatments. This was due to a sufficient quantity and quality of the Tephrosia mulching material. However, moderately labile NaOH-extractable organic P seemed to be depleted in the topsoil due to high P uptake in the TepMu treatment. Thus, nutrient cycling and nutrient balances were improved under the Tephrosia systems. But for long-term P sustainability, there is a belief that a combined use of mulching and mineral P fertiliser is needed.     

Polychlorinated biphenyls and some pesticides in perch (Perca fluviatilis) from inland waters of Latvia

Polychlorinated biphenyls (PCBs), DDT-related substances, hexachlorocyclohexane (HCH) isomers and hexachlorobenzene (HCB) were analysed in perch (Perca fluviatilis) from Latvian lakes and rivers. DDE is present in the highest concentrations in all samples; 62-170 ng/g lipid weight in perch from rural areas and 460 ng/g in perch from the Riga area. Individual dominating PCB congeners were in the range 16-45 ng/g and 200-210 ng/g, respectively. The degree of contamination of rural areas in Latvia of these organohalogen substances is in the same range as in background areas in Sweden. Riga, the major urban area in Latvia, is shown to be more polluted with PCBs than other areas in Latvia.