A bee in the corridor: centering and wall-following

In an attempt to better understand the mechanism underlying lateral collision avoidance in flying insects, we trained honeybees (Apis mellifera) to fly through a large (95-cm wide) flight tunnel. We found that, depending on the entrance and feeder positions, honeybees would either center along the corridor midline or fly along one wall. Bees kept following one wall even when a major (150-cm long) part of the opposite wall was removed. These findings cannot be accounted for by the “optic flow balance” hypothesis that has been put forward to explain the typical bees’ “centering response” observed in narrower corridors. Both centering and wall-following behaviors are well accounted for, however, by a control scheme called the lateral optic flow regulator, i.e., a feedback system that strives to maintain the unilateral optic flow constant. The power of this control scheme is that it would allow the bee to guide itself visually in a corridor without having to measure its speed or distance from the walls.     

Groundwater dependent ecosystems. Part I: Hydroecological status and trends

Groundwater dependent ecosystems (GDEs) include valuable ecosystems such as springs, wetlands, rivers, lakes and lagoons. The protection of these systems and services they provide is highlighted by international agreements, i.e. Ramsar convention on wetlands, and regional legislation, i.e. the European Water Framework Directive. Groundwater provides water, nutrients and a relatively stable temperature. However, the role of groundwater in surface ecosystems is not fully understood. The ecosystem can depend on groundwater directly or indirectly, and the reliance can be continuous, seasonal or occasional. This has implications for the vulnerability of ecosystems, as some may be easily affected by external pressure. Conceptual models and quantitative assessments of how groundwater interacts with the environment are needed. GDEs are also threatened by different land use activities and climate change. Hence, we need to understand how GDEs are affected by changes in groundwater quantity and quality, as severe groundwater changes have been observed in many regions. This study examines key aspects of GDEs (hydrogeology, geochemistry and biodiversity) in order to improve conceptual understanding of the role of groundwater in such ecosystems. The status and baseline of different types of GDEs are discussed, with particular emphasis on past evidence of environmental change and potential thresholds and threats in GDEs in various parts of Europe with different land use, climate and geology.     

Groundwater dependent ecosystems. Part II. Ecosystem services and management in Europe under risk of climate change and land use intensification

Groundwater in sufficient amounts and of suitable quality is essential for potable water supplies, crop irrigation and healthy habitats for plant and animal biocenoses. The groundwater resource is currently under severe pressure from land use and pollution and there is evidence of dramatic changes in aquifer resources in Europe and elsewhere, despite numerous policy measures on sustainable use and protection of groundwater. Little is known about how such changes affect groundwater dependent ecosystems (GDEs), which include various aquatic and terrestrial ecosystems above ground and inside the aquifer. Future management must take this uncertainty into account. This paper focuses on multiple aspects of groundwater science, policy and sustainable management. Examples of current management methods and practices are presented for selected aquifers in Europe and an assessment is made of the effectiveness of existing policies such as the European Water Framework Directive and the Habitat Directive in practice and of how groundwaters and GDEs are managed in various conditions. The paper highlights a number of issues that should be considered in an integrated and holistic approach to future management of groundwater and its dependent ecosystems.     

Wintering Waterbirds and Recreationists in Natural Areas: A Sociological Approach to the Awareness of Bird Disturbance

Disturbance to wintering birds by human recreational activities has become a major concern for managers of many natural areas. Few studies have examined how recreationists perceive their effects on birds, although this impacts their behavior on natural areas. We surveyed 312 users on two coastal ornithological sites in Brittany, France, to investigate their perception of the effects of human activities on wintering birds. The results show that the awareness of environmental issues and knowledge of bird disturbance depends on the socioeconomic characteristics of each user group, both between the two sites and within each site. Results also indicate that, whatever the site and the user group, the vast majority of the respondents (77.6 %) believed that their own presence had no adverse effects on the local bird population. Various arguments were put forward to justify the users’ own harmlessness. Objective information on recreationists’ awareness of environmental issues, and particularly on their own impact on birds, is important to guide managers in their choice of the most appropriate visitor educational programs. We recommend developing global but also specific educational information for each type of user to raise awareness of their own impact on birds.     

The meta-hydroxylation step in the phenylpropanoid pathway: a new level of complexity in the pathway and its regulation

Together in silico and genetic mining approaches have recently designated the CYP98 family of plant cytochromes P450 as the family of enzymes that catalyzes the meta-hydroxylation step in the phenylpropanoid pathway. This meta-hydroxylation is not catalyzed on the free p-coumaric acid as anticipated, but on its conjugates with shikimic, quinic, or phenyllactic acids. While all CYP98s have in common phenol meta-hydroxylase activity, p-coumaroylshikimate remains their preferred substrate. High expression of CYP98s is detected in lignifying tissues in stems, roots, and siliques. The CYP98A3 gene disruption in Arabidopsis thaliana leads to a drastic inhibition of lignin synthesis, cell growth, and plant development. The meta-hydroxylation of phenolic precursors is thus essential for higher plant development. Isolation of coding sequences belonging to the CYP98 family from basil, wheat, and extensive functional analysis of the recombinant enzymes, together with CYP98s from other plant taxa, helps shedding some light on mechanisms of P450s evolution. Most importantly, the occurrence of the meta-hydroxylation on esters of shikimic or quinic acids introduces a new biochemical regulation mechanism in the phenylpropanoid pathway.     

Delimiting soil chemistry thresholds for nickel hyperaccumulator plants in Sabah (Malaysia)

Nickel hyperaccumulator plants have been the focus of considerable research because of their unique ecophysiological characteristics that can be exploited in phytomining technology. Comparatively little research has focussed on the soil chemistry of tropical nickel hyperaccumulator plants to date. This study aimed to elucidate whether the soil chemistry associated with nickel hyperaccumulator plants has distinctive characteristics that could be indicative of specific edaphic requirements. The soil chemistry associated with 18 different nickel hyperaccumulator plant species occurring in Sabah (Malaysia) was compared with local ultramafic soils where nickel hyperaccumulator plants were absent. The results showed that nickel hyperaccumulators in the study area were restricted to circum-neutral soils with relatively high phytoavailable calcium, magnesium and nickel concentrations. There appeared to be a ‘threshold response’ for the presence of nickel hyperaccumulator plants at >20 μg g^?1 carboxylic-extractable nickel or >630 μg g^?1 total nickel, and >pH 6.3 thereby delimiting their edaphic range. Two (not mutually exclusive) hypotheses were proposed to explain nickel hyperaccumulation on these soils: (1) hyperaccumulators excrete large amounts of root exudates thereby increasing nickel phytoavailability through intense rhizosphere mineral weathering; and (2) hyperaccumulators have extremely high nickel uptake efficiency thereby severely depleting nickel and stimulating re-supply of Ni from diffusion from labile Ni pools. It was concluded that since there was an association with soils with highly labile nickel pools, the available evidence primarily supports hypothesis (2).     

Uncertainty in establishing forest reference levels and predicting future forest-based carbon stocks for REDD+

Forest reference levels (FRLs) provide a benchmark for assessing reduced emissions from deforestation and forest degradation (REDD+), and they are central to demonstrate additionality of REDD+. Attaining realistic FRLs, however, is challenging, especially in complex mosaic landscapes. We established FRLs in northern Laos for different reference periods and tested them against actual carbon stock changes. Annual time series of Landsat satellite images were used to capture the subtle changes in carbon stocks in complex landscapes characterized by shifting cultivation. We found that FRLs differ considerably depending on the reference period chosen. Abrupt land-use changes occurred when hybrid maize replaced traditional shifting cultivation and forests, and this invalidated carbon stock trends that would have been predicted had the FRL been projected into the future. We conclude that demonstrating additionality of REDD+ in fast developing areas is difficult and that payment systems rewarding potential emission reductions against hypothetical extrapolation of FRLs are unlikely to be a cost-effective strategy.     

Pedogenesis and nickel biogeochemistry in a typical Albanian ultramafic toposequence

This study aimed at relating the variability of Ni biogeochemistry along the ultramafic toposequence to pedogenesis and soil mineralogy. Hypereutric Cambisols dominate upslope; Cambic Vertisols and Fluvic Cambisols occur downslope. The soil mineralogy showed abundance of primary serpentine all over the sequence. It is predominant upslope but secondary smectites dominate in the Vertisols. Free Fe-oxides are abundant in all soils but slightly more abundant in the upslope soils. Whereas serpentines hold Ni in a similar and restricted range in every soil (approx. 0.3 %), Ni contents in smectites may vary a lot and Mg-rich and Al-poor smectites in the Vertisol could hold up to 4.9 % Ni. Ni was probably adsorbed onto amorphous Fe-oxides and was also exchangeable in secondary smectites. High availability of Ni in soils was confirmed by DTPA extractions. However, it varied significantly along the toposequence, being higher in upslope soils, where Ni-bearing amorphous Fe-oxides were abundant and total organic carbon higher and sensibly lower downslope on the Vertisols: Ni_DTPA varied from 285 mg kg^?1 in the surface of soil I (upslope) to 95.9 mg kg^?1 in the surface of Fluvic Cambisols. Concentration of Ni in Alyssum murale shoots varied from 0.7 % (Hypereutric Cambisols) to 1.4 % (Hypereutric Vertisol). Amazingly, Ni uptake by A. murale was not correlated to Ni_DTPA, suggesting the existence of specific edaphic conditions that affect the ecophysiology of A. murale upslope.