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41.
Meenu Varshney Abhijit Chandra L. K. S. Chauhan Sudhir K. Goel 《Environmental science and pollution research international》2013,20(12):8709-8716
The genotoxic effects of oxidative metabolites of trichloroethylene (TCE), namely chloral hydrate, trichloroacetic acid (TCA), dichloroacetic acid (DCA), and trichloroethanol (TCEOH) were examined in human peripheral blood lymphocytes. In this context, lymphocytes were exposed in vitro to 25, 50, and 100 μg/ml concentrations of these metabolites separately for a period of 48 h and examined for micronucleus (MN) induction through flow cytometer. At 50 μg/ml TCE metabolites, TCA (6.33?±?0.56 %), DCA (5.06?±?0.55), and TCEOH (4.70?±?1.73) induced highly significant (p?<?0.001) frequency of MN in comparison to control (1.03?±?0.40) suggestive of their genotoxic potential. However, exposure of 100 μg/ml of all the metabolites consistently declined the frequencies of MN which in some cases was equable to that of observed at 25 μg/ml. Further, cytotoxicity and cell cycle disturbances were also measured to find out the association of these endpoints with the MN induction. DNA content analysis revealed 3–4-fold elevation of S-phase at all the concentrations tested. Particularly, at 100 μg/ml, treatment elevation of S-phase was significantly (p?<?0.0001) higher as compared to the control. Present findings together with earlier reports indicate that TCE induces genotoxicity through its metabolites. Interaction of these metabolites with DNA, as evident by elevated S-phase, seems to be the major cause of MN induction. However, involvement of spindle disruption cannot be ruled out. This comparative study also suggests that after TCE exposure, the metabolic efficiency of human to generate oxidative metabolites determines the extent of genotoxicity. 相似文献
42.
Shikha Raghuwanshi Tithi Agarwal Amit Yadav MGH Zaidi Yogesh Shouche Reeta Goel 《Chemistry and Ecology》2016,32(6):583-597
Bacterial strains with poly-3-hydroxybutyrate (PHB) degradation potential were isolated from waste yard soil samples of selected industrial sites in Uttarakhand, India, and two microbial consortia were developed, i.e. Consortium I comprises Pseudomonas sp. strain Rb10, Pseudomonas sp. strain Rb11 and Bacillus sp. strain Rb18, and Consortium II is composed of Lysinibacillus sp. strain Rb1, Pseudomonas sp. strain Rb13 and Pseudomonas sp. strain Rb19. The current study involved enrichment selection via liquid and semi-solid media, followed by isolation and screening of bacterial strains using PHB pellets and films. Furthermore, the identification and characterisation was done by triphasic approach. The utilisation of PHB by the characterised strains was confirmed by Fourier transform infrared spectroscopy and scanning electron microscopy. Moreover, the minimum inhibitory concentration of solubilised PHB was found to be 2.5?mg/mL, which was detected through ‘clear zone assay’. Further, the selection and biocompatibility testing of potential isolates were performed for the formation of bacterial consortia. Thus, the present work would provide direct and standardised protocol for screening and selection of potential microbiomes for biodegradation of polymers by overcoming the negative effect of organic solvents. Moreover, indigenously developed consortia would be evaluated for their in situ biodegradation potential against various bioplastic films. 相似文献
43.
Karnatak Gunjan Das Basanta Kumar Sarkar Uttam Kumar Borah Simanku Roy Aparna Parida Pranay Lianthuamluaia Lianthuamluaia Das Archan Kanti Behera Bijay Kumar Pandit Arun Sahoo Amiya Kumar Bhattacharjya Birendra Kumar Chakraborty Sangeeta Mondal Kausik Chandra Purna 《Environmental science and pollution research international》2022,29(50):75431-75440
Environmental Science and Pollution Research - The rapid degradation, overexploitation, and encroachment of floodplain wetlands have led to considerable decline in fish diversity and production... 相似文献
44.
Goyal Keshav Goel Harsh Baranwal Pritika Dixit Aman Khan Fahad Jha Niraj Kumar Kesari Kavindra Kumar Pandey Pratibha Pandey Avanish Benjamin Mercilena Maurya Ankit Yadav Vandana Sinh Rana Suryauday Tanwar Pranay Upadhyay Tarun Kumar Mittan Sandeep 《Environmental science and pollution research international》2022,29(41):61993-62013
Environmental Science and Pollution Research - Environmental mutagens are chemical and physical substances in the environment that has a potential to induce a wide range of mutations and generate... 相似文献
45.
Saxena Abhishek Norton Brian Goel Varun Singh Desh Bandhu 《Environmental science and pollution research international》2022,29(39):58537-58560
Environmental Science and Pollution Research - The successful use of solar energy for cooking requires the systems adopted not only to have technical attributes that conveniently address specific... 相似文献