Enzymatic degradation of poly([R,S] β-hydroxybutyrate)

The synthetic analogue of a bacterially produced polyester, poly(-hydroxybutyrate) (PHB) was synthesized from racemic -butyrolactone using anin situ trimethyl aluminum-water catalyst. The polymer was fractionated into samples differing in molecular weight and isotactic diad content. The latter was closely related to degree of crystallinity. The biodegradation of these fractions were examined by monitoring mass loss over time in the presence of anAlcaligenes faecalis T1 extracellular bacterial poly(-hydroxybutyrate) depolymerase. The fraction with high isotactic diad tacticity content showed little or no degradation over a 50 hour incubation period, whereas the fraction of intermediate isotactic diad content degraded in a continuous steady fashion at a rate that was less than that for bacterial PHB. The low isotactic diad fraction underwent a rapid initial degradation, followed by no further mass loss. The presence of stereoblocks in the polymer structure of the various fractions was an influence on the degree of susceptibility towards degradation and is related to sample crystallinity.     

Plankton inhabiting the surface layer of the southern and southwestern lagoon of New Caledonia

In the southern and southwestern coral reef lagoon of New Caledonia and the adjacent oceanic waters, 42 neuston samples were collected from the upper surface layer (0 to 10 cm) along nine transects from the coast to the coral barrier reef and the open ocean immediately beyond the reef, in March and April 1979. There was a progressive numerical decrease in zooplankton densities from the coast to the reef and from the reef to the open sea. Generally, 80 to 95% of the surface plankton consisted of holoplankton and 5 to 20% of meroplankton. Zooplankton was very abundant in littoral bays with a marked eutrophication. In a few samples collected in very shallow waters close to coral patches, cladocerans were numerous and constituted up to 75% of the total plankton, whilst in other samples collected above greater depths, copepods made up 60 to 85% of the total plankton. In the coral reef lagoon of south and southwest New Caledonia, typically hyponeustonic copepods (pontellids) often comprised 5% of the total copepod populations.     

Age determination and growth of the bonnethead shark Sphyrna tiburo: a comparison of two populations

From July 1982 to December 1986, a study of the age and growth of the bonnethead shark Sphyrna tiburo was conducted in Tampa and Florida Bays, Florida, USA. Tetracycline-injected sharks held in captivity and, to a lesser extent, tagged, released and recaptured, were utilized for validating the annual nature of the rings (herein defined as the narrow, translucent regions) appearing on vertebral centra. The technique was validated for all age groups (0 to 6+yr) included in the study. Marginal increment analysis likewise suggested annual ring formation. The rings formed during the winter, when water temperatures were lowest. Using the vertebral ring aging-technique, von Bertalanffy growth curves for males and females from both tampa and Florida Bays were constructed. Growth of sharks born and held in captivity demonstrated that the male and female growth curves diverge after 1 yr and that mean sizes at age are statistically distinct after 2 yr. In both populations, females grew to larger sizes tha males and apparently are longer-lived. Reproductively mature females from Tampa Bay were significantly larger than those from Florida Bay.     

Sulphur status in some Swedish podzols as influenced by acidic deposition and extractable organic carbon

To evaluate the changes in sulphur pools in response to acidic deposition, two studies were made-one in southwest Sweden where podzolic B horizons originally sampled in 1951 were resampled in 1989. At the Norrliden site, northern Sweden, sulphur pools in control plots were compared to plots that had been subjected to H(2)SO(4) application between 1971 and 1976. The results show that in southwest Sweden neither organic S nor extractable SO(4)(2-) increased significantly over the 38-year period, despite a decreasing pH and a high S deposition. At Norrliden, about 37% of the applied S was still remaining in the upper and central parts of the Bs horizon, most of which was inorganic sulphate. These contrasting results are explained by intrinsic differences in the soil organic carbon status between the sites-in southwest Sweden, organic carbon concentrations were high which inhibited SO(4)(2-) adsorption. Low organic carbon concentrations and high extractable Fe/Al concentrations promoted SO(4)(2-) adsorption and caused a low subsequent SO(4)(2-) desorption rate at the Norrliden site. The results suggest that sulphate adsorption may be an important mechanism which delays the response in soil chemistry to H(2)SO(4) deposition, provided that soil organic carbon concentrations are low. Organic S retention was not shown to be an important S retention mechanism in any of the sites studied.     

Effects of population density and cadmium toxicity on growth and survival of blowflies

The blowfly Lucilia sericata has been reared on artificial diets containing from 0 to 200 mg Cd kg(-1) at population densities ranging from 8 larvae per 4-g diet to 8 larvae per 32-g diet. Peak larval weight, development period, pupal weight, adult-emergence weight, and fecundity were measured. Larvae of blowflies respond to food deprivation by a reduction in larval weight and subsequent pupal and adult size. Contaminating the food supply with cadmium produced similar additive effects, reduced the growth rate, and increased the age of pupation. The results are of importance in determining the effects of environmental pollutants on population dynamics.     

Proximate and elemental composition and energy content of mesopelagic crustaceans from the Eastern Gulf of Mexico

The proximate and elemental chemical compositions of 25 species of pelagic decapod and mysid crustaceans collected from the eastern Gulf of Mexico (27°N; 86°W, 1984 to 1989) was examined. Water level ranged from 63 to 95% and increased slightly with species' increased depth of occurrence. Protein levels were generally high (1.5 to 18.3% wet wt, WW; 27.6 to 62.4% ash-free dry wt, AFDW) and comprised the primary organic component in the majority of species. Protein, both as % WW and % AFDW, decreased with increased depth of occurrence. In contrast to protein, lipid levels were low (0.5 to 8.9% WW; 5.7 to 60.9% AFDW), and increased with increased depth of occurrence. Carbon and nitrogen best mirrored measured lipid and protein levels when considered as non-protein carbon and non-chitin nitrogen, respectively. C:N ratios increased with increased depth, consistent with changes in protein and lipid with depth. Because of their compositional attributes, resident Gulf of Mexico species have a low total wet weight energy content relative to species from more productive regions. Energy content showed no significant trend with depth. Vertical migration patterns were distinctly different between shallow-and deep-living gulf species and these differences were largely responsible for the relationships observed between composition and depth. In migrating species, the protein and nitrogen content were higher, the lipid and carbon contents and C:N ratio lower, than in non-migrating species. Three deep-living species of the genus Acanthephyra were found to be compositionally atypical, resembling shallow, migrating types rather than other deep-living, non-migratory species.     

WORLD PETROLEUM RESOURCE ESTIMATES AND PRODUCTION FORECASTS: IMPLICATIONS FOR GOVERNMENT POLICY

Resource estimates alone will not give advance warning of encroaching production difficulties. An analysis of the general stages in the evolution of petroleum production and discovery and of industry statistics provides an estimate of when the stage characterised by increasing production in the market economy countries outside the United States and Canada will end. The analysis indicates that the year of maximum production will be in the 1990s. Economic growth aggravates the difficulties accompanying the production decline by advancing the date of peak production and by increasing the adjustments that must be made as oil production declines. Des prévisions de ressources à elles seules ne constituent pas des signes précurseurs de difficultés croissantes de production. Une analyse des étapes générales marquant l'evolution de la découverte et de la production du pétrole ainsi que des statistiques industrielles nous permet de prévoir l'époque à laquelle prendra fin la phase de production accrue dans les pays à économie de marché en dehors des Etats-Unis et du Canada. Cette analyse révèle que l'année de production maximale se situera dans les années 90. La croissance économique accentue les difficultés qui accompagnent le déclin de la production de pétrole en avancant la date à laquelle cell-ci atteindra son plafond et en augmentant le nombre d'ajustements qui s'avèrent nécessaires au fur et à mesure qu'elle baisse. Las evaluaciones de recursos no dan por si solas un aviso anticipado de las dificultades de producción. Un análisis de las etapas principales en la evolución del descubrimiento y producción de petróleo y de las estadísticas industriales provee un estimado de cuando va a terminar la etapa caracterizada por un crecimiento de producción en los paises de economias de mercado otros que Estados Unidos y Canada. El análisis indica que el año de producción máxima se hallará en la década del 90. El crecimiento económico agrava las dificultades debidas a la declinación de la producción al adelantar la fecha de máxima producción (pico) y al aumentar los ajustes que deven hacerse cuando la producción de petróleos disminuye.     

Interaction of temperature and salinity on oxygen consumption of the estuarine crab Panopeus herbstii

Marine Biology - The combined effects of temperature and salinity on the rate of oxygen consuption by the estuarine crab Panopeus herbstii Milne-Edwards (Crustacea: Decapoda: Xanthidae) were...