Assessment of heavy metal bioavailability in contaminated sediments and soils using green fluorescent protein-based bacterial biosensors

A green fluorescent protein (GFP)-based bacterial biosensor Escherichia coli DH5alpha (pVLCD1) was developed based on the expression of gfp under the control of the cad promoter and the cadC gene of Staphylococcus aureus plasmid pI258. DH5alpha (pVLCD1) mainly responded to Cd(II), Pb(II), and Sb(III), the lowest detectable concentrations being 0.1 nmol L(-1), 10 nmol L(-1), and 0.1 nmol L(-1), respectively, with 2h exposure. The biosensor was field-tested to measure the relative bioavailability of the heavy metals in contaminated sediments and soil samples. The results showed that the majority of heavy metals remained adsorbed to soil particles: Cd(II)/Pb(II) was only partially available to the biosensor in soil-water extracts. Our results demonstrate that the GFP-based bacterial biosensor is useful and applicable in determining the bioavailability of heavy metals with high sensitivity in contaminated sediment and soil samples and suggests a potential for its inexpensive application in environmentally relevant sample tests.     

基于压电纤维复合材料的风沙冲击力传感器研究∗

沙尘暴是一种多发于沙漠等干旱地区的灾害天气，建（构）筑物常受其影响被侵蚀甚至产生破坏，这种破坏的本质是风沙两相流中沙粒对结构表面冲击产生细微损伤的积累。为了深入探索这种风沙两相流对结构的冲击作用和沙粒冲击力在结构表面的分布规律，研究了采用压电纤维复合材料作为力传感器来测量风沙流场中沙粒冲击力的方法，通过标定试验建立了压电传感器所受沙粒冲击力与其输出电信号之间的数学模型，构建了基于压电纤维复合材料的风沙冲击力传感器。分别采用高频测力天平和压电传感器测量低矮房屋迎风面在风沙风洞试验中所受到的沙粒冲击力，验证压电传感器测量沙粒冲击力的适用性和准确性。结果显示：两种测力方式的结果总体相差在-4.72%~5.80%，两种试验结果吻合良好；建筑物迎风面内侧所受到的沙粒冲击力大于外侧所受到的沙粒冲击力，沙粒冲击力的分布与表面风压力分布具有一定相关关系。研究成果可为结构防风沙灾害的相关研究提供参考。     

Numerical model for static chamber measurement of multi-component landfill gas emissions and its application

The quantitative assessment of landfill gas emissions is essential to assess the performance of the landfill cover and gas collection system. The relative error of the measured surface emission of landfill gas may be induced by the static flux chamber technique. This study aims to quantify effects of the size of the chamber, the insertion depth, pressure differential on the relative errors by using an integrated approach of in situ tests, and numerical modeling. A field experiment study of landfill gas emission is conducted by using a static chamber at one landfill site in Xi’an, Northwest China. Additionally, a two-dimensional axisymmetric numerical model for multi-component gas transport in the soil and the static chamber is developed based on the dusty-gas model (DGM). The proposed model is validated by the field data obtained in this study and a set of experimental data in the literature. The results show that DGM model has a better capacity to predict gas transport under a wider range of permeability compared to Blanc’s method. This is due to the fact that DGM model can explain the interaction among gases (e.g., CH₄, CO₂, O₂, and N₂) and the Knudsen diffusion process while these mechanisms are not included in Blanc’s model. Increasing the size and the insertion depth of static chambers can reduce the relative error for the flux of CH₄ and CO₂. For example, increasing the height of chambers from 0.55 to 1.1 m can decrease relative errors of CH₄ and CO₂ flux by 17% and 18%, respectively. Moreover, we find that gas emission fluxes for the case with positive pressure differential (?P_in-out) are greater than that of the case without considering pressure fluctuations. The Monte Carlo method was adopted to carry out the statistical analysis for quantifying the range of relative errors. The agreement of the measured field data and predicted results demonstrated that the proposed model has the capacity to quantify the emission of landfill gas from the landfill cover systems.

     

The toxicity of the glyphosate herbicide for Pardosa spiders’ predatory activity depends on the formulation of the glyphosate product

Environmental Chemistry Letters - Glyphosate is an herbicide which was previously considered safe for non-target organisms. 825.8 million kilograms of glyphosate-based products were used...     

Vertical distribution and temporal evolution of formaldehyde and glyoxal derived from MAX-DOAS observations: The indicative role of VOC sources

Formaldehyde (HCHO) and glyoxal (CHOCHO) are important oxidization intermediates of most volatile organic compounds (VOCs), but their vertical evolution in urban areas is not well understood. Vertical profiles of HCHO, CHOCHO, and nitrogen dioxide (NO₂) were retrieved from ground-based Multi-Axis Differential Optical Absorption Spectroscopy (MAX-DOAS) observations in Hefei, China. HCHO and CHOCHO vertical profiles prefer to occur at higher altitudes compared to NO₂, which might be caused by the photochemistry-oxidation of longer-lived VOCs at higher altitudes. Monthly means of HCHO concentrations were higher in summer, while enhanced amounts of NO₂ were mainly observed in winter. CHOCHO exhibited a hump-like seasonal variation, with higher monthly-averaged values not only occurred in warm months (July-August) but also in cold months (November-December). Peak values mainly occurred during noon for HCHO but emerged in the morning for CHOCHO and NO₂, suggesting that HCHO is stronger link to photochemistry than CHOCHO. We further use the glyoxal to formaldehyde ratio (GFR) to investigate the VOC sources at different altitudes. The lowest GFR value is almost found in the altitude from 0.2 to 0.4 km, and then rises rapidly as the altitude increases. The GFR results indicate that the largest contributor of the precursor VOC is biogenic VOCs at lower altitudes, while at higher altitudes is anthropogenic VOCs. Our findings provide a lot more insight into VOC sources at vertical direction, but more verification is recommended to be done in the future.