EDTA reduces lead accumulation in Symphytum officinale L. (comfrey) roots

The addition of EDTA in phytoextraction studies has been reported to increase heavy metal accumulation in above-ground parts or to have no negative impact on the overall (root/shoot) accumulation levels in terrestrial plants. At a purely quantitative level, this study assessed the phytoextraction potential of a previously untested high-biomass terrestrial plant, Symphytum officinale L. (comfrey), in the presence of Pb and EDTA. In this hydroponic-based study, we report a small increase in shoot accumulation of Pb with EDTA but, conversely, the presence of EDTA in the nutrient medium markedly reduced the overall quantity of Pb in the plant root by at least 80%. The loss does not appear to be explained by EDTA acting alone, increased transport of Pb to the shoots, or anionic charge repulsion of the [PbEDTA]^2? complex. The elusive action and negative effect of EDTA on Pb accumulation in S. officinale provides additional reasons towards a growing trend away from the use of EDTA as a chelating agent in phytoextraction.     

Overview of the relationship between organizational and workplace factors and injury rates

This paper summarizes data from ten studies examining the relationship between organizational and workplace factors and injury rates. The studies were identified from a systematic literature search, as well as from other sources, and were included if they reported comparisons among at least 20 workplaces. Factors that were examined in at least two studies were identified. Their relationship with injury rates was determined. It was not possible to make quantitative comparisons between studies. We looked for ‘consistency’ or ‘contradiction’ in the relationships. Among the variables ‘consistency’ associated with lower injury rates were: empowerment of the workforce (in general matters); delegation of safety activities; and an active role (in health and safety) of top management. Among the ‘contradictory’ variables were the level and use of discipline for safety violations; and several others that were contradictory only in subgroups of the same study. We discuss the limitations of synthesizing results from the diverse studies.     

Model of a chemostat utilising phenol as inhibitory substrate

A pure culture of Acinetobacter lwoffi was grown in a batch and continuous culture using phenol as the limiting substrate. Five kinetic models were applied to the data to determine the kinetic parameters governing growth of the organisms. A non-linear leastsquares technique was used to fit the data to the different functions. No significant differences were found on statistical basis between the models and so a choice was made on the grounds of mathematical simplicity. The so-called ‘Haldane function’ was chosen as the best and utilised in the model describing the steady-state behaviour of the chemostat. This model takes into account the inhibitory character of phenol and also the maintenance energy of the bacteria. Analysis of local stability shows that one can expect to obtain three steady states of which one is the trivial washout state, another is unstable and the third is a high conversion stable steady state.This model was tested with a pure culture of Acinetobacter lwoffi and phenol inlet concentrations of 200, 500 and 1000 mgl^?1. Good agreement was found between the model and the results of the continuous experiments.     

WATER USE RELATIONSHIPS AND PROJECTION CORRESPONDING WITH REGIONAL GROWTH,SEATTLE REGION1

ABSTRACT: The paper outlines both the methods used and the results obtained in a study of the demand for municipal and industrial water for the Seattle region. The study was made as part of a regional water management study program, one objective of which is to “… identify, quantify, and set priorities for all current and future water uses …”. A basic concept in the study of municipal and industrial water use is that the demand for water is derived from the demand for output and the direct services that water provides. Principal characteristics of the study are: (1) Water use is studied by type - residential, commercial, industrial and public -with identification of factors affecting each; (2) Water demands are studied by season as well as on an annual basis; (3) Projections of future water use are tied directly to projections of economic change in the service area; and (4) The effects of alternative policies on water use are estimated. Water use levels are projected under alternative regional growth assumptions provided by the Puget Sound Governmental Conference, a regional planning agency. Thus, the water use planning is consistent with other regional planning programs in this respect. The results can be varied according to changes in specific factors affecting water use. The factors considered in the present study include: single-family residential lot size, distribution of population between single- and multi-family units, per capita water use by multi-family unit residents, and industrial and commercial water use per employee. An income elasticity of demand was estimated for single-family residential water use.     

Handling intensity and the short- and long-term survival of elephant seals: addressing and quantifying research effects on wild animals

This study addresses the consequences of repeated human handling on the survival of an endangered phocid, the southern elephant seal and the implications for wildlife research. Southern elephant seal pups were repeatedly handled during the first six weeks of their lives. The possibility that such anthropogenic research may have altered the very parameters that were being investigated is a topical and relevant study area that we address here. Our results show that there were no measurable effects on pups that were repeatedly handled and subjected to invasive research methods with respect to survivorship in the short term (the 24-day nursing period) nor in the long term (the first year of life and beyond) and hence fitness one year after handling. In support of this conclusion we were unable to detect any significant differences in the survival rates of the most intensively handled seals and the least intensively handled seals.     

Crown ratio influences allometric scaling in trees

Allometric theories suggest that the size and shape of organisms follow universal rules, with a tendency toward quarter-power scaling. In woody plants, however, structure is influenced by branch death and shedding, which leads to decreasing crown ratios, accumulation of heartwood, and stem and branch tapering. This paper examines the impacts on allometric scaling of these aspects, which so far have been largely ignored in the scaling theory. Tree structure is described in terms of active and disused pipes arranged as an infinite branching network in the crown, and as a tapering bundle of pipes below the crown. Importantly, crown ratio is allowed to vary independently of crown size, the size of the trunk relative to the crown deriving from empirical results that relate crown base diameter to breast height diameter through crown ratio. The model implies a scaling relationship in the crown which reduces to quarter-power scaling under restrictive assumptions but would generally yield a scaling exponent somewhat less than three-quarters. For the whole tree, the model predicts that scaling between woody mass and foliage depends on crown ratio. Measurements on three boreal tree species are consistent with the model predictions.     

A Comparison of Corridors and Intrinsic Connectivity to Promote Dispersal in Transient Successional Landscapes

Low-vagility organisms that specialize on transitory successional habitats may be especially dependent upon habitat connectivity to maintain population viability. We analyzed the theoretical intrinsic connectivity of successional landscapes (i.e., the natural juxtaposition of similar habitats that allows dispersal) as a function of patch geometry coupled with the disperser's habitat specificity. Habitat specialists living in poorly connected landscapes (approximating hexagonal patches) have only a 26.5% chance of colonizing a new site when their resident patch becomes unsuitable. In contrast, generalists living in well connected landscapes can virtually always colonize a new site when needed. We infer from our simulation that for some habitat specialists, such as the rare, endemic Florida scrub lizard (Sceloporus woodi), anthropogenic control of successional dynamics for commercial logging may significantly reduce intrinsic connectivity. Lizard population viability may now depend upon the extrinsic connectivity provided by artificial corridors. However, the use of corridors will not serve as a general solution to the problem of anthropogenically reduced intrinsic connectivity until key logistical design problems have been resolved. Moreover, efforts to enhance intrinsic connectivity by modifying patch geometry may produce undesirable edge effects and conflict with old-growth preservation. Future research should focus on developing spatially explicit corridor models, documenting natural levels of intrinsic connectivity, quantifying anthropogenic disruption of natural connectivity, and describing species-specific mechanisms of inter-patch dispersal.