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171.
B-type esterases in the snail Xeropicta derbentina: an enzymological analysis to evaluate their use as biomarkers of pesticide exposure 总被引:1,自引:0,他引:1
Laguerre C Sanchez-Hernandez JC Köhler HR Triebskorn R Capowiez Y Rault M Mazzia C 《Environmental pollution (Barking, Essex : 1987)》2009,157(1):199-207
The study was prompted to characterize the B-type esterase activities in the terrestrial snail Xeropicta derbentina and to evaluate its sensitivity to organophosphorus and carbamate pesticides. Specific cholinesterase and carboxylesterase activities were mainly obtained with acetylthiocholine (Km = 77.2 mM; Vmax = 38.2 mU/mg protein) and 1-naphthyl acetate (Km = 222 mM, Vmax = 1095 mU/mg protein) substrates, respectively. Acetylcholinesterase activity was concentration-dependently inhibited by chlorpyrifos-oxon, dichlorvos, carbaryl and carbofuran (IC50 = 1.35 × 10−5-3.80 × 10−8 M). The organophosphate-inhibited acetylcholinesterase activity was reactivated in the presence of pyridine-2-aldoxime methochloride. Carboxylesterase activity was inhibited by organophosphorus insecticides (IC50 = 1.20 × 10−5-2.98 × 10−8 M) but not by carbamates. B-esterase-specific differences in the inhibition by organophosphates and carbamates are discussed with respect to the buffering capacity of the carboxylesterase to reduce pesticide toxicity. These results suggest that B-type esterases in X. derbentina are suitable biomarkers of pesticide exposure and that this snail could be used as sentinel species in field monitoring of Mediterranean climate regions. 相似文献
172.
Annette Fliedner Heinz Rüdel Burkhard Knopf Karlheinz Weinfurtner Martin Paulus Mathias Ricking Jan Koschorreck 《Environmental science and pollution research international》2014,21(8):5521-5536
Cadmium, lead, mercury, copper, nickel, zinc, and arsenic were analyzed in suspended particulate matter (SPM), zebra mussels, and bream sampled yearly under the program of the German Environmental Specimen Bank (ESB) in the rivers Rhine, Elbe, Danube, Saar, Mulde, and Saale and in Lake Belau. Temporal and spatial trends were analyzed, correlations between metal levels in different specimen types assessed, and sampling sites ranked according to their metal levels by calculating a Multi-Metal Index (MMI) for every specimen type and site. SPM: Highest metal loads were detected in Mulde, Saale, and Elbe right downstream of the Saale confluence. In the Elbe, metal loads in SPM were mostly highest in the upper and middle section of the river while in Rhine and Saar concentrations increased downstream. Temporal trends since 2005 were detected only at three sites. Zebra mussel: MMIs were highest in the tidal section of the Elbe and the lower Rhine and lowest in Lake Belau and the upper Danube. Different temporal trends were detected since the early 1990s depending on site and metal. Bream: As, Pb, Cu, and Hg were analyzed in muscle tissue and Pb, Cd, Cu, and Zn in liver. For both tissues, MMIs were highest in Mulde and Saale and the lower and middle Elbe. Since the early 1990s, Hg, Pb, and Cu decreased in bream muscle at many sites while As increased at 6 of the 17 sites. The findings indicate that Hg, Pb, and Cu have obviously decreased in many freshwater ecosystems in recent years, whereas As and Ni levels have increased at several sites. Metal levels and temporal trends mostly differed between the specimen types under investigation and only few correlations between specimen types were detected. This underlines the importance of including different components of an ecosystem when assessing its environmental quality. 相似文献
173.
Werner Kördel Hemda Garelick Bernd M. Gawlik Nadia G. Kandile Willie J. G. M. Peijnenburg Heinz Rüdel 《Environmental science and pollution research international》2013,20(5):2810-2827
Substance-related monitoring is an essential tool within environmental risk assessment processes. The soundness of policy decisions including risk management measures is often directly related to the reliability of the environmental monitoring programs. In addition, monitoring programs are required for identifying new and less-investigated pollutants of concern in different environmental media. Scientifically sound and feasible monitoring concepts strongly depend on the aim of the study. The proper definition of questions to be answered is thus of pivotal importance. Decisions on sample handling, storage and the analysis of the samples are important steps for the elaboration of problem-oriented monitoring strategies. The same applies to the selection of the sampling sites as being representative for scenarios to be investigated. These steps may become critical to handle for larger international monitoring programs and thus trigger the quality of their results. This study based on the work of an IUPAC (International Union of Pure and Applied Chemistry) task group addresses different kinds and approaches of substance-related monitoring of different compartments of soil, groundwater and surface water, and discusses their advantages and limitations. Further important aspects are the monitoring across policies and the monitoring data management using information systems. 相似文献
174.
Hofer N Alexou M Heerdt C Löw M Werner H Matyssek R Rennenberg H Haberer K 《Environmental pollution (Barking, Essex : 1987)》2008,154(2):241-253
The effect of free-air ozone fumigation and crown position on antioxidants were determined in old-growth spruce (Picea abies) trees in the seasonal course of two consecutive years (2003 and 2004). Levels of total ascorbate and its redox state in the apoplastic washing fluid (AWF) were increased under double ambient ozone concentrations (2xO3), whilst ascorbate concentrations in needle extracts were unchanged. Concentrations of apoplastic and symplastic ascorbate were significantly higher in 2003 compared to 2004 indicating a combined effect of the drought conditions in 2003 with enhanced ozone exposure. Elevated ozone had only weak effects on total glutathione levels in needle extracts, phloem exudates and xylem saps. Total and oxidised glutathione concentrations were higher in 2004 compared to 2003 and seemed to be more affected by enhanced ozone influx in the more humid year 2004 compared to the combined effect of elevated ozone and drought in 2003 as observed for ascorbate. 相似文献
175.
Haberer K Jaeger L Rennenberg H 《Environmental pollution (Barking, Essex : 1987)》2006,139(2):224-231
In the present field study the role of ascorbate in scavenging the harmful atmospheric trace gases O3 and NO2 was examined. For this purpose ascorbate contents were determined in needles of adult Scots pine trees (Pinus sylvestris L.) during three consecutive years. Ascorbate contents were correlated with ambient tropospheric O3 and NO2 concentrations and with meteorological parameters. The results showed a strong correlation of atmospheric O3 but not of atmospheric NO2 concentrations with the apoplastic content of ascorbate during the seasonal course. Ascorbate contents in needle extracts did not correlate with ambient trace gas concentrations. In the apoplastic space, but not in needle extracts ascorbate contents correlate highly significantly with global radiation. From these results it is assumed that apoplastic ascorbate in Scots pine needles is adapted to the actual atmospheric O3 concentration to mediate immediate detoxification of O3, while the atmospheric O3 concentration itself is largely dependent on light intensity. 相似文献
176.
Fabrice Helfenstein Murielle Podevin Heinz Richner 《Behavioral ecology and sociobiology》2010,64(4):557-565
Sperm competition exerts strong selection on males to produce spermatozoa with an optimal morphology that maximizes their
fertilization success. Long sperm were first suggested to be favored because they should swim faster. However, studies that
investigated the relationship between sperm length and sperm competitive ability or sperm swimming velocity yielded contradictory
results. More recently, ratios of the different sections of a spermatozoon (the head, midpiece, and flagellum) were suggested
to be more crucial in determining swimming velocity. Additionally, sperm ability to remain and survive in the female storage
organs may also influence fertilization success, so that optimal sperm morphology may rather maximize sperm longevity than
velocity. In this study, we investigated how sperm morphology is related to sperm velocity and sperm longevity in the house
sparrow Passer domesticus. Sperm velocity was found to be correlated with head/flagellum ratio. Sperm with small heads relative to their flagellum showed
higher swimming velocity. Additionally, shorter sperm were found to live longer. Finally, we found sperm morphological traits
to vary substantially within males and the head/flagellum ratio to be unrelated to total sperm length. We discuss the hypothesis
that the substantial within-male variation in sperm morphology reflects a male strategy to produce a diversity of sperm from
long, fast-swimming to short, long-living sperm to maximize their fertilization success in a context of sperm competition. 相似文献
177.
Tamas Komives Gabor Gullner Heinz Rennenberg John E. Casida 《Water, Air, & Soil Pollution: Focus》2003,3(3):277-283
The ability of poplar trees to resist chemical stress caused by chloroacetanilide herbicides was studied. Detached leaves of Lombardy poplar (Populus nigra L.) were exposed to seven chloroacetanilide herbicides via uptake through the cut petiole. The leaves showed high tolerance against the phytotoxicity of these compounds. Their tolerance was further enhanced by treatment with 2-oxothiazolidine-4-carboxylic acid (OTC, a precursor of the amino acid cysteine). High levels of glutathione (GSH) and GSH S-transferase (GST) activity were detected in poplar leaves. Treatments with chloroacetanilide herbicides left the GSH content in the leaves unchanged but strongly induced the GST activity. In contrast, in OTC-treated leaves increased GSH contents were measured, but GST activities remained unchanged. It therefore appears that a highly active and inducible GSH-conjugation Phase II detoxification system in their leaves may make poplar trees useful for phytoremediation of soils contaminated with chloroacetanilide herbicides. Based on these findings a phytoremediation project using different poplar hybrids at a site heavily polluted with such compounds in Hungary has been launched. 相似文献
178.
Schröder HC Brandt D Schlossmacher U Wang X Tahir MN Tremel W Belikov SI Müller WE 《Die Naturwissenschaften》2007,94(5):339-359
Biomineralization, biosilicification in particular (i.e. the formation of biogenic silica, SiO2), has become an exciting source of inspiration for the development of novel bionic approaches following “nature as model”.
Siliceous sponges are unique among silica forming organisms in their ability to catalyze silica formation using a specific
enzyme termed silicatein. In this study, we review the present state of knowledge on silicatein-mediated “biosilica” formation
in marine sponges, the involvement of further molecules in silica metabolism and their potential application in nanobiotechnology
and medicine.
Werner E. G. Müller dedicated this study to Prof. Vera Gamulin (Rudjer Boskovic Institute, Zagreb, Croatia) in honour of her
unique contributions in molecular evolution. 相似文献
179.
Heinz L. Engelbrecht 《Environment international》1981,6(1-6)
Dust build-up on adjacent surfaces is often an unwanted effect in industrial processes handling pulverized materials. In the case of electrostatic precipitation, build-up of dust on the collecting surface is the purpose of the process. Removal of this build-up is sometimes difficult, but necessary to maintain the continuity of the process. Dry-process methods for dust removal include rapping by impact.It is the intent of this paper to provide information on the general subject of dust removal from plates, on rapping systems used in electrostatic precipitators, and on measurements and order of magnitude of rapping forces.Rapping acceleration test results obtained in the laboratory are expanded to cover full-scale collecting surface plates.Effects of plate design, plate height and length, method of support, rapping, hammer weight, and lift are analyzed. 相似文献
180.