Behavioural response to gradually declining oxygen concentration by Baltic Sea macrobenthic crustaceans

Behavioural responses to decreasing oxygen concentrations were studied in the amphipods Monoporeia affinis (Lindström) and Pontoporeia femorata Krøyer and the isopod Saduria entomon (L.), all benthic macrofauna species from the Baltic Sea. Infrared time-lapse video recording was used. Seven oxygen concentrations were studied, starting near saturation (about 90%), followed by about 65, 49, 33, 16, 8 and 5% oxygen saturation, with each oxygen level maintained for 24?h. M. affinis was more active than P. femorata. The duration of swimming activity of M. affinis was shorter at ≤33% oxygen saturation compared with at the two highest levels. The duration of swimming by P. femorata was longest at 90, 65 and 5% oxygen saturation. For both amphipods the frequency of swimming activity was highest at 5% oxygen saturation. Above 7% oxygen saturation, S. entomon spent most of its time in the sediment, whereas at levels ≤7% it was most often found on the sediment surface. Both amphipods were predominantly night active (nocturnal), whereas no diurnal variation in behaviour was detected for S. entomon. It is suggested that the amphipods minimise the costs associated with obtaining oxygen as well as their risk of predation by moving little on or above the sediment until a lower critical oxygen level is reached. Below this level, they show an oxygen-seeking behaviour. S. entomon also hid in the sediment for as long as possible, suddenly coming to the surface once the oxygen concentration became intolerably low. Its behaviour is also indicative of a trade-off between predation risk and obtaining sufficient oxygen. It is suggested that the change in behaviour shown at about 33% oxygen saturation could lead to a long-term alteration in community structure in the deeper parts of the northern Baltic proper, whereas the change at about 8% oxygen saturation reflects a more acute, short-term response.     

气候变化与紫外线B辐射对北极苔原和极地荒漠生态系统的影响重要发现和扩展总结

引言 北极已成为对当前气候波动和预计的全球变暖增强的影响进行评估的重要地区.原因有以下几个方面:①在过去几十年中北极经历了大幅度的变暖过程(温度平均升高3℃,而在许多地区温度升高了4～5℃);②气候预测表明气候呈现持续变暖趋势,在2080年之前年平均气温升高4～5℃;③近期的气候变暖正在影响北极的环境和经济,这些影响还会加大,并对生活方式、文化及生态系统造成影响;④北极的变化可能会影响到地球上的其它地区.     

巴西雨林自然保护区河流中杀虫剂残毒:对水生生物和人类健康的影响评价

1998年11月、1999年3月和2000年元月分别在巴西雨林自然保护区河流的水体、沉积物和鱼体中检测杀虫剂残毒,每种杀虫剂浓度与基于急性毒性测试的生态毒理基准进行了比较,并且还与规定标准进行比较,以确定它们对水生生物和人类潜在影响的程度.在所有的7个调查地点都检测出杀虫剂及其代谢产物,在水体以及沉积物样品中检测出总共27种杀虫剂及其代谢物的残毒,鱼类富集其中某些最持久稳固的残毒.水体和沉积物中检测出的杀虫剂残毒浓度表明水生生物保护的问题需要引起重视,水体中的几种杀虫剂含量超过了巴西以及欧盟当局规定的指标,表明对人类的健康也有影响.     

气候变化对景观和区域过程的影响及其对气候系统的反馈

北极生态系统的生物和物理过程会在不同的时间、空间尺度上对地球生态系统产生反馈作用,并与之相互影响.气候变化对北极地区的影响及其对全球气候系统的反馈主要存在着四种潜在机制反照率改变、生态系统对温室气体的排放或吸收、甲烷类温室气体的排放、影响海洋暖流淡水量的增长.这些反馈机制在某种程度上是由生态系统的分布和特征,尤其是大规模植被区域变化来控制的.通过少量全年的CO2通量测量表明,目前在地理分布上碳源区要比碳汇区要多.根据目前现有的关于CH4排放源地信息表明,景观规模上的CH4排放量对北极地区的温室效应平衡至关重要.北极地区的能量和水量平衡在变化的气候下,也是一个很重要的反馈机制.植被密度以及分布范围的增加会导致反射率的下降,因而会使地表吸收更多的能量.其效果可能会抵消由于极地沙漠地带向极地苔原带的的转化,或极地苔原带向极地森林带的转化,而造成的植被总净初级生产力碳沉降能力的提高而引起的负反馈.永久冻土带的退化对示踪气体动力学有着很复杂的影响.在不连续的永久冻土带地区,升温将会导致其完全消失.依赖于当地水文条件,温室气体排放可能由于气候环境变的干燥或湿润而使得其通量有所变化.总的来说,影响反馈的各种过程复杂的相互作用,以及这些过程随着时间地点的变化,加之数据的缺乏,又会在陆地生态系统气候变化对气候系统产生反馈作用的净效应估计上,产生许多的不确定性,这种不确定性将会影响到一些反馈的大小和方向.     

Genetic variability and level of differentiation in North Sea and Baltic Sea populations of the green alga<Emphasis Type="Italic"> Cladophora rupestris</Emphasis>

Cladophora rupestris is a perennial filamentous macroalga belonging to the Chlorophyta. It is widely distributed on both sides of the northern Atlantic Ocean and penetrates into the brackish Baltic Sea down to ca. 4 psu salinity. In this paper we present evidence for genetic differentiation of a Baltic form of this marine alga. We assessed genetic structure within and among 11 populations ranging along a salinity gradient from the Norwegian coast to the northern Baltic Sea proper. Samples of 328 individuals were studied using starch-gel protein electrophoresis to evaluate genetic variability and interpopulation differentiation based on allozymes. Of 11 loci examined, only one was polymorphic. For this locus, encoding superoxide dismutase (SOD-3), a total of seven alleles were distinguished. We found two genetically differentiated groups of populations of C. rupestris, one Baltic Sea group and one North Sea group, with a distinct border in the southern Kattegat near the entrance to the Baltic Sea. The genetic differentiation for SOD-3, expressed as pairwise F_ST values between the populations, was generally higher within the Baltic Sea group (0.10-0.43) than within the North Sea group (0.05-0.10); in the latter group also fewer pairs of populations differed significantly. Pairs of populations from different groups had the highest F_ST values (0.20-0.60). Hierarchical analysis of variance showed that 29.6% of the total variation in the SOD-3 locus was explained by variation between the two groups, while only 4.2% was explained by variation among the populations within the groups. The remaining variation (66.2%) was found within the populations.     

Do plant density, nutrient availability, and herbivore grazing interact to affect phlorotannin plasticity in the brown seaweed Ascophyllum nodosum

Plants have different strategies to cope with herbivory, including induction of chemical defences and compensatory growth. The most favourable strategy for an individual plant may depend on the density at which the plants are growing and on the availability of nutrients, but this has not been tested previously for marine plant–herbivore interactions. We investigated the separate and interactive effects of plant density, nutrient availability, and herbivore grazing on the phlorotannin (polyphenolic) production in the brown seaweed Ascophyllum nodosum. Seaweed plants grown at low or high densities were exposed either to nutrient enrichment, herbivorous littorinid gastropods (Littorina obtusata), or a combination of nutrients and herbivores in an outdoor mesocosm experiment for 2 weeks. Seaweeds grown at a low density tended to have higher tissue nitrogen content compared to plants grown at a high density when exposed to elevated nutrient levels, indicating that there was a density dependent competition for nitrogen. Herbivore grazing induced a higher phlorotannin content in plants grown under ambient, but not enriched, nutrient levels, indicting either that phlorotannin plasticity is more costly when nutrients are abundant or that plants responded to herbivory by compensatory growth. However, there were no significant interactive or main effects of plant density on the seaweed phlorotannin content. The results indicate that plants in both high and low densities induce chemical defence, and that eutrophication may have indirect effects on marine plant–herbivore interactions through alterations of plant chemical defence allocation.     

Pesticide pollution remains severe after cleanup of a stockpile of obsolete pesticides at Vikuge, Tanzania

High levels of DDT residues and hexachlorocyclohexanes (HCHs) were found in soil, well water, and surface water around a collapsed pesticide storage shed at Vikuge Farm, Tanzania. Residues of DDT and HCHs were found at three soil depths down to 50 cm. Surface soil samples contained up to 28% total DDT and 6% total HCH residues. Water samples had concentrations of up to 30 microg L(-1) of organochlorine pesticides. Other compounds detected were aldrin, azinphos-methyl, carbosulfan, gamma-chlordane, chlorprofam, heptachlor, hexazinone, metamitron, metazachlor, pendimethalin, and thiabendazole. Although the visible remains of pesticides have been removed, the remaining soil is itself hazardous waste and poses a risk to the environment and the inhabitants of the surrounding villages. These findings show the necessity to follow up the environmental situation at former storage sites of obsolete stocks of pesticides, and that the environmental problems are not necessarily solved by removing the visible remains.     

Leaching of pesticides through normal-tillage and low-tillage soil--a lysimeter study. I. Isoproturon

Isoproturon is a herbicide, which was used in Denmark against grass weeds and broad-leaved weeds until 1998. Isoproturon has frequently been detected in ground water monitoring studies. Leaching of isoproturon (N,N-dimethyl-N'-(4-(1-methylethyl)-phenyl)urea) and its metabolites, N'-(4-isopropylphenyl)-N-methylurea and N'-(4-isopropylphenyl)urea was studied in four lysimetres, two of them being replicates from a low-tillage field (lysimeter 3 and 4), the other two being replicates from a normal tillage field (lysimeter 5 and 6). In both cases the soil was a sandy loam soil with 13-14% clay. The lysimetres had a surface area of 0.5 m2 and a depth of 110 cm. Lysimeter 3 and 4 were sprayed with unlabelled isoproturon while lysimeter 5 and 6 was sprayed with a mixture of 14C-labelled and unlabelled isoproturon. The total amount of isoproturon sprayed onto each lysimeter was 63 mg, corresponding to 1.25 kg active ingredient per ha. The lysimeters were sprayed with isoproturon on October 26, 1997. The lysimetres were installed in an outdoor system in Research Centre Flakkebjerg and were thus exposed to normal climatic conditions of the area. A mean of 360 l drainage water were collected from lysimeter 3 and 4 and a mean of 375 litres from lysimeter 5 and 6. Only negligible amounts of isoproturon and its primary metabolites were found in the drainage water samples, and thus no significant difference between the two lysimeter sets was shown. In a total of 82 drainage water samples, evenly distributed between the four lysimetres isoproturon was found in detectable amounts in two samples and N'-(4-isopropylphenyl)urea was found in detectable amounts in two other samples. The detection limit for all the compounds was 0.02 microg/l. 48% and 54% of the added radioactivity were recovered from the upper 10 cm soil layer in lysimeter 5 and 6, respectively, and 17 and 14% from 10-20 cm's depth. By extraction first with an aquatic CaCl2 solution 0.49% of the added radioactivity was extracted from the upper 10 cm layer in lysimeter 5. In the subsequent extraction with acetonitril, 1.19% of the added radioactivity was extracted. In lysimeter 6, upper 10 cm, 0.2% were extracted with water and 0.56% were extracted with acetonitril. Below 10 cm's depth no measurable amounts could be extracted.     

Effects on the function of Arctic ecosystems in the short- and long-term perspectives

Historically, the function of Arctic ecosystems in terms of cycles of nutrients and carbon has led to low levels of primary production and exchanges of energy, water and greenhouse gases have led to low local and regional cooling. Sequestration of carbon from atmospheric CO2, in extensive, cold organic soils and the high albedo from low, snow-covered vegetation have had impacts on regional climate. However, many aspects of the functioning of Arctic ecosystems are sensitive to changes in climate and its impacts on biodiversity. The current Arctic climate results in slow rates of organic matter decomposition. Arctic ecosystems therefore tend to accumulate organic matter and elements despite low inputs. As a result, soil-available elements like nitrogen and phosphorus are key limitations to increases in carbon fixation and further biomass and organic matter accumulation. Climate warming is expected to increase carbon and element turnover, particularly in soils, which may lead to initial losses of elements but eventual, slow recovery. Individual species and species diversity have clear impacts on element inputs and retention in Arctic ecosystems. Effects of increased CO2 and UV-B on whole ecosystems, on the other hand, are likely to be small although effects on plant tissue chemisty, decomposition and nitrogen fixation may become important in the long-term. Cycling of carbon in trace gas form is mainly as CO2 and CH4. Most carbon loss is in the form of CO2, produced by both plants and soil biota. Carbon emissions as methane from wet and moist tundra ecosystems are about 5% of emissions as CO2 and are responsive to warming in the absence of any other changes. Winter processes and vegetation type also affect CH4 emissions as well as exchanges of energy between biosphere and atmosphere. Arctic ecosystems exhibit the largest seasonal changes in energy exchange of any terrestrial ecosystem because of the large changes in albedo from late winter, when snow reflects most incoming radiation, to summer when the ecosystem absorbs most incoming radiation. Vegetation profoundly influences the water and energy exchange of Arctic ecosystems. Albedo during the period of snow cover declines from tundra to forest tundra to deciduous forest to evergreen forest. Shrubs and trees increase snow depth which in turn increases winter soil temperatures. Future changes in vegetation driven by climate change are therefore, very likely to profoundly alter regional climate.     

Assessment of the importance of sorption for steroid estrogens removal during activated sludge treatment

Distribution coefficients (K(d)) between water and activated sludge particles (f(oc)=27.7+/-0.1%) were measured for the steroid estrogens (SE), estrone (E1), 17beta-estradiol (E2) and 17alpha-ethinylestradiol (EE2) in batch experiments. Experimental concentration levels ranged from environmentally realistic low ng/l to the high microg/l. In this range K(d)s were independent of their water concentration. The experimentally obtained K(d)s (with 95% confidence intervals) were 402+/-126 l/kg, 476+/-192 l/kg and 584+/-136 l/kg for E1, E2 and EE2, respectively. K(d)s were used to estimate the fraction of the total SE concentration that is expected to be sorbed in the activated sludge treatment tanks of a typical STP assuming equilibrium conditions. Assuming a suspended solids concentration of 4 g/l dissolved solids (ds), it was estimated that 61+/-9%, 66+/-13% and 70+/-6% of the total concentration of E1, E2 and EE2, respectively, would be sorbed during activated sludge treatment. The fraction of the SEs that was expected to be sorbed to suspended sludge particles in the effluents from a typical Danish STP was estimated to be only 0.20+/-0.06%, 0.24+/-0.10% and 0.29+/-0.07% of the total concentration of E1, E2 and EE2, respectively, at a suspended solids concentration of 5 mg/lds. For a typical STP the removal of steroid estrogens with excess sludge was estimated to be only 1.5-1.8% of the total loading if equilibrium conditions exists. Sorption is therefore not important for the fate of SEs in STPs compared to biodegradation.