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211.
Michael A. Schmidt Javier M. Gonzalez Jonathan J. Halvorson Ann E. Hagerman 《Chemosphere》2013,90(6):1870-1877
Polyphenols including tannins comprise a large percentage of plant detritus such as leaf litter, and affect soil processes including metal dynamics. We tested the effects of tannins on soil metal mobilization by determining the binding stoichiometries of two model polyphenols to Al(III) and Fe(III) using micelle-mediated separation and inductively coupled plasma optical emission spectroscopy (ICP-OES). By fitting the data to the Langmuir model we found the higher molecular weight polyphenol (oenothein B) was able to bind more metal than the smaller polyphenol (epigallocatechin gallate, EGCg). For example, oenothein B bound 9.43 mol Fe mol?1, while EGCg bound 4.41 mol of Fe mol?1. Using the parameters from the binding model, we applied the Langmuir model for competitive binding to predict binding for mixtures of Al(III) and Fe(III). Using the parameters from the single metal experiments and information about polyphenol sorption to soils we built a model to predict metal mobilization from soils amended with polyphenols. We tested the model with three natural soils and found that it predicted mobilization of Fe and Al with r2 = 0.92 and r2 = 0.88, respectively. The amount of metal that was mobilized was directly proportional to the maximum amount of metal bound to the polyphenol. The secondary parameter in each model was the amount of weak organically chelated Fe or Al that was in the soil. This study provides the first compound-specific information about how natural polyphenols interact with metals in the environment. We propose a model that is applicable to developing phytochelation agents for metal detoxification, and we discuss how tannins may play a role in metal mobilization from soils. 相似文献
212.
An analysis of the population genetics of the meiofaunal polychaete Petitia amphophthalma Siewing, 1956, in which the RAPD-PCR method was applied to 103 individuals from eight populations, some of them very far
apart (Atlantic: Florida, Tenerife, France; Mediterranean: two Greek islands, Tunisia; Red Sea: Egypt), gave closely reproducible
results. In the band patterns produced with 13 decamer primers, a total of 195 genetic characters was detected. The data were
evaluated by a number of methods, including the cluster programs UPGMA, WPGMA and neighbour-joining. The detected genetic
distances between the populations vary between 58.9 and 66.6, but 97% of the genetic characters, although polymorphic, are
found in at least two populations and usually in all the others as well. Phenograms of the analyses find four population clusters
[Florida, France (Atlantic), the Mediterranean and Tenerife]. They are, however, not completely congruent and show low bootstrap
values at the junction points of the clusters (with the exception of the Tenerife cluster). Mediterranean P. amphophthalma form a cohesive population, although within it the genetic distances are graded in parallel with the geographic distances
between the sites. The colonization of Tenerife, an island of relatively recent volcanic origin, can be taken as evidence
that this meiofaunal species can become dispersed not only along coastlines but also across expanses of open water. However,
the severely restricted variability of these populations implies that in this case a founder effect has operated, and that
transport over open water is not a routine event but extremely rare. The absence of the species on the Australian coast and,
for instance, on the Galapagos Islands indicates that there has been no continuous gene flow across the oceans. The idea that
all the populations investigated belong to one cosmopolitan species is discussed.
Received: 17 October 1997 / Accepted: 15 April 1998 相似文献
213.
Comparison of efficacy of three extractants to solubilize glomalin on hyphae and in soil 总被引:1,自引:0,他引:1
Glomalin, a glycoprotein produced by arbuscular mycorrhizal (AM) fungi, is a major component of the humus fraction of soil organic matter. Glomalin is extracted from soil and hyphae of AM fungi by using sodium citrate at 121 degrees C in multiple 1-h cycles, but extensive extraction does not solubilize all glomalin in all soils. Efficacies of 100 mM sodium salts of citrate, borate or pyrophosphate (pH 9.0, 121 degrees C) were tested for two 1-h cycles for hyphae from four AM fungal isolates and four 1-h cycles for seven soils from four US geographic regions. Residual soil glomalin was examined by pyrophosphate extraction of soils previously extracted with citrate or borate followed by extraction of all soils after treatment with NaOH. Hyphal extracts were compared using Bradford-reactive total protein (BRTP) values, and extracts from soils were compared using BRTP, percentage C and C weight. No difference among extractants was detected for AM fungal isolates or across soils. The residual glomalin across soils for extractants contained the following percentages of the total BRTP: pyrophosphate, 14%; borate, 17%; and citrate, 22%. Comparisons among individual soils indicated that pyrophosphate extracted significantly more BRTP (10-53%) than borate or citrate in six soils and borate was equal to pyrophosphate in one soil. Extraction with borate should be compared with pyrophosphate before initiating an experiment. For routine extractions of ca. 85% of the glomalin across a variety of soils, sodium pyrophosphate appears to be equal to or better than borate and better than citrate. 相似文献
214.
Coastal protection strategies increasingly have to take into account the effects of climate change. At present, engineering and natural science models that assess the impact of global climatic transformations on regional coastal zones and their protection structures remain rather detached from the knowledge and insights of regional practitioners. The main thesis of this contribution, using a case study from the North Sea Coast of Germany, is that innovative coastal protection requires not only interdisciplinary research but transdisciplinary collaboration in order to develop a viable adaptation strategy. The investigation of the social dimension of climate change and coastal protection strategies, using qualitative interviews with organized regional stakeholders, climate researchers and coastal engineers, as well as a representative public survey, contributes to a comprehensive understanding of regional perceptions with respect to climate change and coastal protection. 相似文献
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218.
Scattering layers beyond the Continental Shelf, in deep water, were often observed during cruises with the charter vessel of the Regional Fisheries Survey in West Africa. The layers were located at depths of 100 to 350 m during daytime; at night, they generally ascended to the surface. They consisted mainly of myctophids and small squids. In this report, results of first observations on deep-scattering layers of Sardinella aurita (Cuv. et Val.) are given. The layers occurred off Mauritania during the months of hydrological transition, characterized by low water-temperatures. In these months, S. aurita were rarely caught over the shelf. Apparently, they migrate north and south in deep water, and were not detected by commercial vessels. The environmental conditions and the difficulties for the industrial fishery during this transitional period are briefly described. the temporal and spatial occurrence of deep-scattering S. aurita is given, and diurnal vertical migrations are shown, together with examples of echo records. Results of fishing operations made in the layer, and some biological observations on S. aurita are presented. 相似文献
219.
220.
We analysed maternal serum human chorionic gonadotropin (hCG) in 16 pregnancies with fetal abdominal wall defects previously identified prenatally by elevated maternal serum alpha-fetoprotein (AFP) or at birth. The AFP levels had a mean of 6·38 MOM (range 0·34–15·65), as expected with these defects. The hCG levels had a mean of 1·82 MOM (range 0·23–4·11). The hCG levels in five pregnancies (31·25 per cent) were above 2·30 MOM. Elevated levels of hCG may be associated with fetal abdominal wall defects. 相似文献