Contrasting effects of sulfide and thiosulfate on symbiotic CO2-assimilation of Phallodrilus leukodermatus (Annelida)

Experiments were conducted in order to specify the reductants responsible for the carbon dioxide fixation of the symbiotic sulfur bacteria in the gutless marine oligochaete Phallodrilus leukodermatus (Annelida) from shallow calcareous sediments in Bermuda. Carbon dioxide-uptake rates were suppressed by S⁼ and stimulated by S₂O₃ ⁼. Individuals which hosted bacteria containing reserve energy substances maintained a high short-term CO₂-uptake activity, while bacteria in worm homogenates and in worms treated with an antibiotic (Baypen) did not show any significant metabolic activity. Absolute uptake rates in P. leukodermatus were usually considerably higher than those reported for other animals harbouring prokaryotic sulfuroxidizing symbionts. Utilization of thiosulfate rather than sulfide is compatible with the preferred occurrence of the worms around the redox discontinuity layer and has been confirmed in other thiobiotic animals. Sulfur stored in the symbiotic bacteria appears to be oxidized to sulfate and be excreted when the worms are held under energy-limited conditions. The data emphasize the complexity of the possible metabolic pathways involved in the oxidation of reduced-sulfur compounds by bacterial symbionts in marine invertebrates.     

REGIONAL SEWERING AND GROUNDWATER QUALITY IN THE SOUTHERN SAN JOAQUIN VALLEY1

ABSTRACT: Sewage effluent is commonly disposed of on land in arid regions, and the majority usually recharges the groundwater. Few evaluations have been made of the effect of effluent disposal on groundwater quality in the west. Groundwater hydrologists are rarely involved with treatment plant design and operation or effluent disposal in the San Joaquin Valley, California. The author's purpose is to evaluate the effect of regional sewering programs on groundwater quality in this valley. Research was conducted on past studies of sewage effluent disposal. An extensive water sampling program was undertaken near the Fresno Sewage Treatment Plant. Chloride contents were used to trace recharged effluent. The extent of recharged effluent was delineated and traced for about ten miles. Sampling between the plant and the urban Fresno area indicated a high probability of large-scale leakage of raw sewage into the groundwater.