An amniocentesis was performed at 13.3 weeks' gestation for advanced maternal age. A mosaic sex chromosome pattern was found: of 50 cells examined, 34 had a 45,X karyotype. In 14 cells with a modal number of 46, a recognizable Y was substituted by a small non-fluorescent marker. C-banding identified the marker as an isodicentric in 12 cells. In two cells, the non-fluorescent marker appeared to be monocentric and looked like a non-fluorescent del (Yq), but could have been an isodicentric Y with inactivation of one of the centromeres. Two cells with a modal number of 47 showed two copies of the monocentric marker. Fluorescent in situ hybridization with an alpha satellite Y-specific centromeric probe confirmed the Y-chromosome origin of the markers and allowed for more accurate prenatal diagnostic information. 相似文献
Steam gasification of dehydrochlorinated poly(vinyl chloride) (PVC) or activated carbon was carried out in the presence of
various alkali compounds at 3.0 MPa and 560°C–660°C in a batch reactor or in a semi-batch reactor with a flow of nitrogen
and steam. Hydrogen and sodium carbonate were the main products, and methane and carbon dioxide were the minor products. Yields
of hydrogen were high in the presence of sodium hydroxide and potassium hydroxide. The acceleration effect of the alkali compounds
on the gasification reaction was as follows: KOH > NaOH > Ca(OH)2 > Na2CO3. The rate of gasification increased with increasing partial steam pressure and NaOH/C molar ratio. However, the rate became
saturated at a molar ratio of NaOH/C greater than 2.0. 相似文献
The mass food poisoning incident yusho occurred in Japan in 1968. It was caused by the ingestion of rice bran oil contaminated with polychlorinated biphenyls and various dioxins and dioxin-like compounds including polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDFs). Notably, PCDFs were found to contribute to approximately 65% of the total toxicity equivalent in the blood of yusho patients. Lipophilic dioxins are retained in the body for a longer period than previously estimated. Victims suffered from characteristic skin manifestations associated with non-specific systemic symptoms, neurological symptoms, and respiratory symptoms. The severe symptoms seen in the initial phase subsequently faded, but recently, improvements have scarcely been observed. The Yusho Group has been researching treatments for this condition. Several clinical trials with chelating agents or dietary fibers aimed at accelerating the excretion of compounds. While some treatments increased dioxin excretion, none provided satisfactory symptom relief. Concurrently, various phytochemicals and herbal extracts have been found to possess biological activities that suppress dioxin-induced toxicity via aryl hydrocarbon receptor or activate the antioxidant nuclear factor-erythroid 2-related factor-2 (NRF2) signal pathway, making them promising therapeutic candidates. Here, we summarize the current status of yusho and findings of clinical trials for yusho patients and discuss the treatment prospects.
It is known that aeration reduces rapidly the concentration of organic matter in leachate. However, the oxygen flow rate required to attain a certain reaction rate of organic matter should be carefully estimated. In this study, using the oxygen ratio (the ratio of oxygen flow rate by aeration to oxygen consumption rate of waste layer) as a parameter, the reaction rate of organic matter in leachate from landfilled incineration ash and incombustible waste upon aeration was evaluated. Total organic carbon (TOC) in the leachate was reduced rapidly when the oxygen ratio was high. The decomposition rate exceeded the elution rate of TOC in the leachate from the waste layer for several days when the oxygen ratio was above 102. The results indicate that the oxygen ratio can be used as a parameter for the aeration operation in actual landfill sites, to rapidly stabilize organic matter in leachate. 相似文献
Loss of zooxanthellae (dinoflagellate Symbiodinium) from corals will sometimes lead to mass mortality of corals. To detect and quantify Symbiodinium released from corals, we developed a zooxanthellae “trap” and a quantitative PCR (qPCR) system with Symbiodinium clades A–F-specific primer sets. The trap was attached to a branch or the surface of several wild stony corals, and the water
samples within the traps, including released Symbiodinium, were subjected to qPCR. All tested corals released clade C Symbiodinium at estimates of ~5,900 cells h−1 cm−2 of coral surface. Although all tested Pocillopora eydouxi harboured both clades C and D, some of these colonies released only clade C or released a lesser amount of clade D than that
in the tissues. Our Symbiodinium quantification system revealed that wild hermatypic corals constantly release Symbiodinium to the environment. Our result suggests that some corals may discharge certain clades of Symbiodinium alternatively. 相似文献
Extracellular DNA is omnipresent in aquatic environments and is thought to be a genetic material for horizontal gene transformation between microorganisms. We studied the impact of gamma irradiation on the transformation efficiency (transformants number per ng of DNA per ml) of extracellular DNA. Plasmid pEGFP as a model extracellular DNA was irradiated by gamma rays. The transformation efficiency decreased with the increase in radiation dose. A total dose of 10Gy is normally not lethal for microorganisms but certainly affects the transformation efficiency of extracellular DNA. The decrease in the efficiency would be induced by strand breaks of extracellular DNA because the yield of both single-strand breaks (SSBs) and double-strand breaks (DSBs) increased with the increase in radiation dose. The relative transformation efficiency of SSBs and DSBs to that of covalently closed circles (CCCs) was 30.3% and 0.2%, respectively. This impact on natural transformation suggests an inability of microorganisms to acquire new characteristics which should be normally acquired. 相似文献
The present research investigated the relationship between nitrate–nitrogen (NO3–N) in river water and the land use/land cover (hereafter, land use) in the Teshio River watershed located in northern Hokkaido
island to understand the effect of human activities such as agriculture, forestry, industry, and urbanization in the drainage
basin on the river ecosystem quality and services. River water was sampled at nine points seasonally during a 2-year period
and the nutrients concentration was measured. Land use profiles were estimated at two spatial scales, riparian and sub-catchment,
for each sampling station. The spatial pattern of water quality in the Teshio River showed increased NO3–N levels associated with agriculture and urban expansion, and forest reduction in the watershed. Land use at the riparian
scale closely reflected that at the sub-catchment scale, which masked the unique riparian buffer effect on the river water
condition. The increased agricultural and reduced forest area in the riparian zone, especially in the upper middle reach,
could be a possible reason for a decline of ecosystem service for the provisioning of clean water and habitat for aquatic
organisms. Measures towards sustainable and more nature-friendly agricultural management are necessary in the area to protect
the Teshio River ecosystem and its ecosystem services. 相似文献
Effects of acute γ-irradiation were investigated in the aquatic microcosm consisting of green algae (Chlorella sp. and Scenedesmus sp.) and a blue–green alga (Tolypothrix sp.) as producers; an oligochaete (Aeolosoma hemprichi), rotifers (Lecane sp. and Philodina sp.) and a ciliate protozoan (Cyclidium glaucoma) as consumers; and more than four species of bacteria as decomposers. At 100 Gy, populations were not affected in any taxa. At 500–5000 Gy, one or three taxa died out and populations of two or three taxa decreased over time, while that of Tolypothrix sp. increased. This Tolypothrix sp. increase was likely an indirect effect due to interspecies interactions. The principal response curve analysis revealed that the main trend of the effects was a dose-dependent population decrease. For a better understanding of radiation risks in aquatic microbial communities, effect doses of γ-rays compared with copper, herbicides and detergents were evaluated using the radiochemoecological conceptual model and the effect index for microcosm. 相似文献
Most landfilled plastic waste is a mixture or is in the form of composites with incombustible wastes such as glass, metals,
and ceramics. After hydrothermal treatment, including a steam-explosion process, the separation of mixed waste (MW) into organic
and inorganic substances becomes easy. However, the effect of hydrothermal pretreatment on the subsequent liquefaction of
organic substances from MW is not obvious. In this study, the effects on the liquefaction of polystyrene and high-density
polyethylene are discussed. Moreover, optimum conditions for the liquefaction of organic substances from hydrothermally treated
MW are identified. By means of this hydrothermal pretreatment, including the steam-explosion process, polystyrene and high-density
polyethylene can be significantly converted to oil by liquefaction at 300°–400°C. In comparison with liquefaction of hydrothermally
pretreated mixed waste (HMW) at 300°–400°C with a batch type reactor, the yield of oil increases significantly on liquefaction
using a semi-batch type reactor. It is considered that the radical chain and termination reactions among the radicals from
HMW were inhibited in the semi-batch type reactor. On liquefaction of HMW in a semi-batch reactor, the conversion of HMW to
oil was enhanced on increasing the liquefaction temperature to 350°C and the holding time to 60 min.
Chemical Feedstock Recycling & Other Innovative Recycling Techniques 6 相似文献