Development of an ELISA for the detection of bromoxynil in water

For development of an indirect competitive enzyme-linked immunosorbent assay (ELISA) for the nitrile herbicide bromoxynil, the polyclonal antibodies raised against 2,6-dibromo-4-cyano-phenoxyacetic acid (hapten) conjugated to bovine serum albumin (BSA) by the N-hydroxysuccinimide-activated ester method. Antiserum with a sufficiently high titer to provide the determinations of targeted compounds was obtained only 77 days after the primary immunization. Antiserum A2 was applied to the residual analysis of some water samples, under optimized ELISA condition, the quantitative working range was from 10 to 500 ppb with a limit of detection of 5 ppb. Cross-reactivity to structurally similar agrochemicals and related chemicals was determined. The antiserum showed little cross-reactivity with 2,6-dibromophenol and bromoxynil octanoate ester which have a dibromophenol group as common structure, but showed no cross-reactivity with other herbicides. Each water sample (river water, tap water, purified water, and bottled water) had a matrix effect and was investigated by adding Tween20 in the assay buffer. These four kinds of water samples were fortified with bromoxynil at several concentration levels and were directly analyzed with only dilution with an equal volume of antiserum solution, the mean recovery was 102.3%, and the mean coefficient of variation was 5.96%. The proposed ELISA turned out to be a powerful tool for monitoring of residual bromoxynil in water samples at trace level.     

常减压蒸馏装置改造中的火灾危险性研究

应用火灾危险性预先分析法,对化工生产中的常减压蒸馏装置改造施工的火灾危险性进行分析,制定出相应的消防安全对策,以确保改造中的消防安全.     

事故树分析系统设计与老空区透水分析

通过计算机编程,开发了事故树分析系统,可以方便、准确地进行事故树分析计算,规范地绘制事故树图形,提高了事故分析的速度和质量;利用该系统,对老空区积水透水事故进行事故树分析,并根据分析结果,提出了该类透水事故的预防方案。     

磷酸三苯酯(TPP)和磷酸三(2-氯异丙基)酯(TCPP)诱导人胚肾细胞HEK293凋亡的机制研究

磷系阻燃剂对人体的潜在毒性作用引起了国内外研究者的广泛关注。肾脏是机体重要的排毒器官,若肾脏细胞受损,可能影响肾脏功能的正常发挥。本研究以人胚肾细胞HEK293为研究对象,结合传统毒理学实验,筛选出磷酸三苯酯(TPP)及磷酸三(2-氯异丙基)酯(TCPP)诱导人胚肾细胞HEK293凋亡的关键靶标基因p53。在此基础上采用分子对接模拟和光谱法分析发现,TPP和TCPP分别以嵌插方式和沟槽方式结合p53-DNA,改变基因片段的框架结构,启动分子起始事件,通过影响相关基因(Bax、Hrk、Bcl-2和Bad)的表达量,导致线粒体途径释放cyt c,最终激活Caspase 7实现细胞凋亡。研究结果阐明了此类污染物诱导凋亡的作用机制,为毒害化学品的污染防控提供理论依据。     

静电除尘在球团竖炉烟尘治理上的应用

介绍了球团竖炉烟尘治理的改造经验,总结了电除尘工程概况及效果。     

探讨面向21世纪环境保护发展的压力

按目前形势发展预测,近些年内我国环境状况难以得到根本改善,由于能源和水资源紧缺,下世纪初环境保护将承受巨大压力。根据数据分析,改善消费结构、推行清洁生产、推动经济国际化进程有利于降低对环境的压力     

合肥台CTS-1分析系统网络组建

本文阐述了利用UNIX和WIN98/2000互联的局域网技术,对CTS-1系统进行优化配置,组建CTS-1网络分析系统,实现CTS-1系统数据共享,提高地震分析的效率,确保数据备份。利用代理软件用一个MODEM让各个子机同时上网,同时充分利用台站现有的计算机设备,最大限度的实现地震监控、速报、分析以及地震研究的同时开展。     

农林生物质在含铬废水处理中的应用

农林生物质作为低成本吸附剂在环境污染治理中日益受到重视。综述了农林生物质在含铬废水处理中的应用研究进展，分析了生物质处理含铬废水的机理、影响因素，指出了生物质法处理含铬废水的发展方向。     

Human exposure and health risk of alpha-, beta-, gamma- and delta-hexachlorocyclohexane (HCHs) in Tianjin, China

The dynamic exposures to HCHs of individuals born between the years 1913 and 1993 in Tianjin have been simulated by connecting a fugacity model (IV) with a multimedia exposure model. Ingestion is the most important pathway for human beings to take up HCHs, and concentrations in the human body correlate with body weight changes. Accumulations of HCHs were derived assuming that the degradation in human body behaved linearly. The health risk of exposure to HCHs was measured using cancer risk and loss of life expectancy (LLE), and LLE was modified from its original definition to incorporate a dynamic calculation that takes variances in exposure into account. Monte-Carlo simulations were run to analyze the uncertainties of the model.