Protected Area Management in Jordan

/ Protected area management is increasingly important throughout the world, particularly in less developed countries and arid regions. The Middle East, includingJordan, has important and unique resources due to its varied topography and climate. In Jordan, the protected areas are privately, rather than publicly, managed, and this provides for a unique and somewhat challenging management effort. The purpose of this paper is to review the establishment and administration of Jordan's protected areas with particular emphasis on the challenges of multiple administrative and legislative layers, departmental working relationships, and a paucity of funding. Interviews with government and nongovernmental experts in Jordan, coupled with a review of pertinent academic and planning literature, served as the information base for this study. Despite new legislative and administrative initiatives, results reveal important and continuing challenges for Jordan. Recommendations include completion of protected area inventories, government wide institutional strengthening, partnering with organizations and the public, as well as legislative reexamination.     

SALSA: a simulation tool to assess ecological sustainability of agricultural production

In order to assess the ecological sustainability of agricultural production systems, there is a need for effective tools. We describe an environmental systems analysis tool called SALSA (Systems Ana/ysis for Sustainable Agriculture). It consists of substance/material flow models in which the simulation results are interpreted with life-cycle assessment methodology. The application of SALSA is demonstrated in a case study in which three different ways of producing pigs are compared with respect to energy input and the environmental impacts of global warming, eutrophication, and acidification. The scenario that combined a low-protein diet without soy meal with an improved manure-management technique with low nitrogen losses was the best for all impact categories studied. The strength of the SALSA models was their capacity to capture consequences of management options that had an influence on several processes on a farm, which enabled the type of complex studies we describe.     

Use of questionnaires and an expert panel to judge the environmental consequences of chemical spills for the development of an environment-accident index

Assessing the environmental consequences of a chemical accident is a complex task. To date, the methods used to evaluate the environmental effects of an acute release of a chemical have often been based on measurements of chemical and physical variables deemed to be important, such as the concentration of the chemical. However, a broader strategy is needed to predict the environmental consequences of potential accidents during the planning process. An Environment-Accident Index (EAI), a simple tool based on such a strategy, has been developed to facilitate the consideration of a multitude of influential variables. The objectives of this study were to evaluate whether questionnaire-based expert panel's judgements could provide useful data on the environmental consequences of chemical spills, and an effective basis for further development of the EAI. As expected, the judgements did not agree perfectly, but they do give rough indications of the environmental effects, and highlight consistent trends that should be useful inputs for planning, prevention and decontamination processes. The different accidents were also judged to have caused everything from minor to very major effects in the environment, implying that a wide range of accident scenarios were represented in the material and covered by the EAI. Therefore, questionnaires and expert panel judgements can be used to collect useful data for estimating the likely environmental consequences of chemical accidents and for further development of the EAI.     

Semilobar holoprosencephaly in a 46,XY female fetus

We report the prenatal echographic diagnosis of holoprosencephaly (HPE) at 11 weeks' gestation. Fetopathological examination revealed an unusual variant of semilobar HPE with middle interhemispheric fusion associated with sex-reversal: 46,XY normal male karyotype, normal external and internal female genitalia and streak gonads. Copyright © 2001 John Wiley & Sons, Ltd.     

Quantitative determination of de-conjugated chrysene metabolites in fish bile by HPLC-fluorescence and GC--MS

Two analytical methods have been evaluated for quantitative determination of de-conjugated chrysene metabolites in fish bile. High performance liquid chromatography-fluorescence (HPLC-F) and gas chromatography-mass spectrometry (GC--MS) were compared regarding instrumental and overall limits of detection (LOD) as well as recoveries for the following nine chrysene compounds: 1-, 2,- 3-, 4- and 6-hydroxychrysene (1-, 2-, 3-, 4- and 6-OH-chr), 1,2-dihydroxy-1,2-dihydrochrysene (1,2-DHD-chr), 3,4-dihydroxy-3,4-dihydrochrysene (3,4-DHD-chr), 5,6-dihydroxy-5,6-dihydrochrysene (5,6-DHD-chr) and chrysene. Instrumental LODs were comparable for the two methods whereas the overall LOD was better for HPLC-F. Recoveries varied per chrysene compound for both HPLC-F (62-107%) and GC-MS (48-124%). In vivo formed chrysene metabolites were studied in the bile of Atlantic cod (Gadus morhua) exposed to chrysene (1 mg/kg) via intra-peritoneal (i.p.) and inter-muscular (i.m.) injection. Total amounts of chrysene metabolites were three times higher in i.p. compared to i.m. exposed cod bile, but the relative distribution of determined metabolites was very similar. 1,2-DHD-chr was the most prominent metabolite in de-conjugated bile and constituted more than 88% of the total chrysene metabolites. Additional chrysene metabolites formed were 3,4-DHD-chr and 1-, 2-, 3- and 4-OH-chr. K-region chrysene metabolites (oxidation at carbons 5 and 6) were not detected and seem to be a less favoured biotransformation route. The two methods were applied and evaluated for analysis of chrysene metabolites in two bile reference materials (BCR 720 and 721) and a limited number of field exposed cods.     

Direct and interactive effects of enemies and mutualists on plant performance: a meta-analysis

Plants engage in multiple, simultaneous interactions with other species; some (enemies) reduce and others (mutualists) enhance plant performance. Moreover, effects of different species may not be independent of one another; for example, enemies may compete, reducing their negative impact on a plant. The magnitudes of positive and negative effects, as well as the frequency of interactive effects and whether they tend to enhance or depress plant performance, have never been comprehensively assessed across the many published studies on plant-enemy and plant-mutualist interactions. We performed a meta-analysis of experiments in which two enemies, two mutualists, or an enemy and a mutualist were manipulated factorially. Specifically, we performed a factorial meta-analysis using the log response ratio. We found that the magnitude of (negative) enemy effects was greater than that of (positive) mutualist effects in isolation, but in the presence of other species, the two effects were of comparable magnitude. Hence studies evaluating single-species effects of mutualists may underestimate the true effects found in natural settings, where multiple interactions are the norm and indirect effects are possible. Enemies did not on average influence the effects on plant performance of other enemies, nor did mutualists influence the effects of mutualists. However, these averages mask significant and large, but positive or negative, interactions in individual studies. In contrast, mutualists ameliorated the negative effects of enemies in a manner that benefited plants; this overall effect was driven by interactions between pathogens and belowground mutualists (bacteria and mycorrhizal fungi). The high frequency of significant interactive effects suggests a widespread potential for diffuse rather than pairwise coevolutionary interactions between plants and their enemies and mutualists. Pollinators and mycorrhizal fungi enhanced plant performance more than did bacterial mutualists. In the greenhouse (but not the field), pathogens reduced plant performance more than did herbivores, pathogens were more damaging to herbaceous than to woody plants, and herbivores were more damaging to crop than to non-crop plants (suggesting evolutionary change in plants or herbivores following crop domestication). We discuss how observed differences in effect size might be confounded with methodological differences among studies.     

Mapping epistemic cultures and learning potential of participants in citizen science projects

The ever‐widening scope and range of global change and interconnected systemic risks arising from people–environment relationships (social‐ecological risks) appears to be increasing concern among, and involvement of, citizens in an increasingly diversified number of citizen science projects responding to these risks. We examined the relationship between epistemic cultures in citizen science projects and learning potential related to matters of concern. We then developed a typology of purposes and a citizen science epistemic‐cultures heuristic and mapped 56 projects in southern Africa using this framework. The purpose typology represents the range of knowledge‐production purposes, ranging from laboratory science to social learning, whereas the epistemic‐cultures typology is a relational representation of scientist and citizen participation and their approach to knowledge production. Results showed an iterative relationship between matters of fact and matters of concern across the projects; the nexus of citizens’ engagement in knowledge‐production activities varied. The knowledge‐production purposes informed and shaped the epistemic cultures of all the sampled citizen science projects, which in turn influenced the potential for learning within each project. Through a historical review of 3 phases in a long‐term river health‐monitoring project, we found that it is possible to evolve the learning curve of citizen science projects. This evolution involved the development of scientific water monitoring tools, the parallel development of pedagogic practices supporting monitoring activities, and situated engagement around matters of concern within social activism leading to learning‐led change. We conclude that such evolutionary processes serve to increase potential for learning and are necessary if citizen science is to contribute to wider restructuring of the epistemic culture of science under conditions of expanding social‐ecological risk.     

All-in-one whole exome sequencing strategy with simultaneous copy number variant,single nucleotide variant and absence-of-heterozygosity analysis in fetuses with structural ultrasound anomalies: A 1-year experience

Objective

We performed a 1-year evaluation of a novel strategy of simultaneously analyzing single nucleotide variants (SNVs), copy number variants (CNVs) and copy-number-neutral Absence-of-Heterozygosity from Whole Exome Sequencing (WES) data for prenatal diagnosis of fetuses with ultrasound (US) anomalies and a non-causative QF-PCR result.

Methods

After invasive diagnostics, whole exome parent-offspring trio-sequencing with exome-wide CNV analysis was performed in pregnancies with fetal US anomalies and a non-causative QF-PCR result (WES-CNV). On request, additional SNV-analysis, restricted to (the) requested gene panel(s) only (with the option of whole exome SNV-analysis afterward) was performed simultaneously (WES-CNV/SNV) or as rapid SNV-re-analysis, following a normal CNV analysis.

Results

In total, 415 prenatal samples were included. Following a non-causative QF-PCR result, WES-CNV analysis was initially requested for 74.3% of the chorionic villus (CV) samples and 45% of the amniotic fluid (AF) samples. In case WES-CNV analysis did not reveal a causative aberration, SNV-re-analysis was requested in 41.7% of the CV samples and 17.5% of the AF samples. All initial analyses could be finished within 2 weeks after sampling. For SNV-re-analysis during pregnancy, turn-around-times (TATs) varied between one and 8 days.

Conclusion

We show a highly efficient all-in-one WES-based strategy, with short TATs, and the option of rapid SNV-re-analysis after a normal CNV result.     

Developing safety indicators for preventing offshore oil and gas deepwater drilling blowouts

An important question with respect to the Macondo blowout is whether the accident is a symptom of systemic safety problems in the deepwater drilling industry. An answer to such a question is hard to obtain unless the risk level of the oil and gas (O&G) industry is monitored and evaluated over time. This article presents information and indicators from the Risk Level Project (RNNP) in the Norwegian O&G industry related to safety climate, barriers and undesired incidents, and discusses the relevance for deepwater drilling. The main focus of the major hazard indicators in RNNP is on production installations, whereas only a limited number of incident indicators and barrier indicators are related to mobile drilling units. The number of kicks is an important indicator for the whole drilling industry, because it is an incident with the potential to cause a blowout. Currently, the development and monitoring of safety indicators in the O&G industry seems to be limited to a short list of “accepted” indicators, but there is a need for more extensive monitoring and understanding. This article suggests areas of extensions of the indicators in RNNP for drilling based on experience from the Macondo blowout. The areas are related to schedule and cost, well planning, operational aspects, well incidents, operators’ well response, operational aspects and status of safety critical equipment. Indicators are suggested for some of the areas. For other areas, more research is needed to identify the indicators and their relevance and validity.     

Sustainable Aquaculture: Are We Getting There? Ethical Perspectives on Salmon Farming

Aquaculture is the fastest growing animal producing sector in the world and is expected to play an important role in global food supply. Along with this growth, concerns have been raised about the environmental effects of escapees and pollution, fish welfare, and consumer health as well as the use of marine resources for producing fish feed. In this paper we present some of the major challenges salmon farming is facing today. We discuss issues of relevance to how to ensure sustainability, by focusing on animal production systems, breeding approaches, sources for feed ingredients, and genetic engineering strategies. Other crucial issues such as animal welfare, environmental quality, and ethics are elaborated with regard to relevance for the sustainability of aquaculture. Additionally, we comment on socio-economic distributive implications by intellectual property rights (IPR) strategies on access to genetic material and traceability. To improve sustainability of salmon farming we suggest that there is a need for new approaches to guide research, for identification of ethical issues, and for engaging stakeholders in resolving these challenges.