Screening of agonistic activities against four nuclear receptors in wastewater treatment plants in Japan using a yeast two-hybrid assay

To assess the potential endocrine disruptive effects through multiple nuclear receptors(NRs),especially non-steroidal NRs,in municipal wastewater,we examined the agonistic activities on four NRs(estrogen receptor α,thyroid hormone receptor α,retinoic acid receptor α and retinoid X receptor α) of untreated and treated wastewater from municipal wastewater treatment plants(WWTPs) in Japan using a yeast two-hybrid assay.Investigation of the influent and effluent of seven WWTPs revealed that agonistic activities...     

Measurements of short-lived cosmic-ray-produced radionuclides in rainwater

Cosmic-ray-produced (CP) nuclides with half-lives shorter than 21h were measured in rainwater by ultra-low-background gamma spectrometry at Ogoya Underground Laboratory. As levels of CP nuclides are extremely low and their half-lives are very short, quick sampling methods for a large volume of rainwater and rapid chemical separations by ion exchange method were developed. The nuclides measured were short-lived (24)Na, (28)Mg, (38)S, (38)Cl, (39)Cl, as well as nuclides with longer half-lives (7)Be and (22)Na. The number of atoms of CP nuclides in rainwater were evaluated to range from 30 to 1500L(-1) for (24)Na (n=16, mean; 520 [6.7mBqL(-1)]), 80 to 600L(-1) for (28)Mg (n=13, mean; 260 [2.4mBqL(-1)]), 400 to 1900L(-1) for (39)Cl (n=6, mean; 1200 [250mBqL(-1)]), 1x10(6) to 4x10(7)L(-1) for (7)Be (n=16, mean; 7x10(6) [1.05BqL(-1)]) and 2x10(3) to 1x10(5)L(-1) for (22)Na (n=9, mean; 2x10(4) [0.2mBqL(-1)]).     

Bacterial community succession during the enrichment of chemolithoautotrophic arsenite oxidizing bacteria at high arsenic concentrations

To generate cost-effective technologies for the removal of arsenic from water, we developed an enrichment culture of chemolithoautotrophic arsenite oxidizing bacteria (CAOs) that could effectively oxidize widely ranging concentrations of As(III) to As(V). In addition, we attempted to elucidate the enrichment process and characterize the microbial composition of the enrichment culture. A CAOs enrichment culture capable of stably oxidizing As(III) to As(V) was successfully constructed through repeated batch cultivation for more than 700 days, during which time the initial As(III) concentrations were increased in a stepwise manner from 1 to 10-12 mmol/L. As(III) oxidation activity of the enrichment culture gradually improved, and 10-12 mmol/L As(III) was almost completely oxidized within four days. Terminal restriction fragment length polymorphism analysis showed that the dominant bacteria in the enrichment culture varied drastically during the enrichment process depending on the As(III) concentration. Isolation and characterization of bacteria in the enrichment culture revealed that the presence of multiple CAOs with various As(III) oxidation abilities enabled the culture to adapt to a wide range of As(III) concentrations. The CAOs enrichment culture constructed here may be useful for pretreatment of water from which arsenic is being removed.     

Why did the project fail to achieve its objectives in some villages? The experience of the Upland Settlement Project (USP) in Bangladesh

By sampling an upland settlement project (USP) village, this study examined why the project failed to achieve the planned objectives in some villages, and what livelihood strategies villagers (planters) have adopted to sustain their livelihoods. The paper uses data obtained by qualitative and quantitative methods. The USP was formulated with the objectives of settling 50 landless and marginal tribal families in a project village and introducing agroforestry and rubber plantations for short- and long-term socioeconomic development of planters, in a participatory manner. There are now only 15 planters in the project village and, although agroforestry has progressed well, only 30 to 40% of poor quality rubber trees remain. Planter participation in project activities was limited to wage labour, indicating that the USP failed to adequately achieve all its proposed objectives. In the face of project failure, planters have adopted diversified strategies to maintain their livelihoods. Several shortcomings caused failure of the project, such as the inability of project staff members to organize planters, failure to create awareness among planters about project benefits, lack of regular project money disbursement, sociocultural ignorance, and lack of social capital among planters. Recommendations are made for securing effective participation of local people to assure successful and sustainable project outcomes. Lessons learned will be helpful in formulating future development programmes in this country and elsewhere.     

Nematode community composition in hydrothermal vent and adjacent non-vent fields around Myojin Knoll,a seamount on the Izu-Ogasawara Arc in the western North Pacific Ocean

In contrast to specific large benthic invertebrates in chemosynthetic ecosystems such as hydrothermal vents, meiofaunal communities in such habitats have been reported to have strong taxonomic overlap with meiofauna in the adjacent “normal” environments. However, meiofauna have only recently been included in studies of those environments and detailed information on these communities is still rare. This is especially true in the Northwest Pacific Ocean, even though there are many seamounts with active vents in the calderas of the region. Nematode community composition at the genus level in sediments from a hydrothermal vent field in the caldera of Myojin Knoll (32°06′N, 139°52′E, depth 1,300 m), a seamount on the Izu-Ogasawara Arc, Japan, was investigated for the first time and was compared with adjacent non-vent areas inside and outside the caldera. Multivariate analyses showed that the composition of nematodes in the hydrothermal field was significantly different from that in the non-hydrothermal fields around the caldera. However, the common genera, such as Oxystomina, Pareudesmoscolex, Desmoscolex, and Microlaimus were found in two, or all three vent fields while their rank contributions differed among the three fields. When the data from Myojin Knoll were compared with those from other deep-sea vent environments in different regions (e.g., North Fiji Basin, East Pacific Rise, Mid-Atlantic Ridge), the nematode composition in the vent field of the Myojin caldera was more similar to that of the non-vent fields around the caldera than the composition in vent fields of other regions. These data from the Northwest Pacific Ocean also suggest the absence of long-range transport systems and local adaptations for meiofauna in hydrothermal vent fields.     

Chlorine promotes antimony volatilization in municipal waste incineration

Antimony volatilization in municipal waste incineration was studied. Two municipal waste samples and antimony(III) oxide (Sb₄O₆) were heated to 500°C and 700°C in an air stream in a quartz furnace. The volatilization of Sb₄O₆ occurred more at 700°C that at 500°C. Conversely, antimony volatilization form municipal waste was stronger at 500°C than at 700°C. This implies that antimony from municipal waste is volatilized as chloride instead of oxide. The chlorine sources for antimony chlorination, a gas-phase reaction involving hydrochloric acid and a solid-phase reaction of inorganic chlorine, e.g., CaCl₂, were compared. Only the solid-phase reaction could offer enough active chlorine to induce chlorination of antimony oxide. Received: July 2, 1998 / Accepted: January 28, 1999     

Driver exposure to particulate matter in Bangkok

The aims of this study were to determine the particulate matter with aerodynamic diameters > or = 2.5 microm (PM2.5) and 2.5-10 microm (PM10-2.5) exposure levels of drivers and to analyze the proportion of elemental carbon (EC) and organic carbon (OC) in PM2.5 in Bangkok, Thailand. Four bus routes were selected. Measurements were conducted over 10 days in August (rainy season) 2008 and 8 days in January (dry season) 2009. The mean PM2.5 exposure level of the Tuk-tuk drivers was 86 microg/m3 in August and 198 microg/m3 in January. The mean for the non-air-conditioned bus drivers was 63 microg/m3 in August and 125 microg/m3 in January. The PM2.5 and PM10-2.5 exposure levels of the drivers in January were approximately twice as high as those in August. The proportion of total carbon (TC) in PM2.5 to the PM2.5 level in August (0.97 +/- 0.28 microg/m3) was higher than in January (0.65 +/- 0.13 microg/m3). The proportion of OC in the TC of the PM2.5 in August (0.51 +/- 0.08 microg/m3) was similar to that in January (0.65 +/- 0.07 microg/m3). The TC exposure by PM25 in January (81 +/- 30 microg/m3) remained higher than in August (56-21 microg/m3). The mean level of OC in the PM2.5 was 29 +/- 13 microg/m3 in August and 50 +/- 24 microg/m3 in January. In conclusion, the PM exposure level in Bangkok drivers was higher than that in the general environment, which was already high, and it varied with the seasons and vehicle type. This study also demonstrated that the major component of the PM was carbon, likely derived from vehicles.     

Analysis of building environment assessment frameworks and their implications for sustainability indicators

Green construction is gaining increasing attention in the global context. However, the construction of sustainable green buildings and environments involves different tools and systems and diverse perspectives. Therefore, the development of environmental assessment tools is an important task for managing green housing and green building projects. In this paper, we discuss the benefits, limitations, and future directions of the assessment framework. There are four characteristics of building environmental assessment, i.e., comprehensiveness, design guideline, signaling, and communication tools, which afford both benefits and limitations. We illustrate the role of the assessment framework as a hub promoting integration of diverse knowledge, as a design guideline encouraging better design and action, as signaling environmentally friendly design and action, and as a communication tool. On the other hand, there are limitations, such as the use of a mixture of quantitative and qualitative measures, ambiguity of weighing, lack of financial evaluation, and lack of involvement of diverse disciplines and stakeholders. To develop an effective assessment framework, the following three factors must be considered: knowledge, power, and implementation. We propose that knowledge innovation, a credible approach for a salient solution, and collective action represent the future challenges of the assessment framework.     

Phosphorus recovery from secondary effluent and side-stream liquid in a sewage treatment plant using zirconium-loaded saponified orange waste

Zirconium was loaded onto orange waste, a cheap and available agricultural waste in Japan, to investigate the feasibility of its utilization for phosphorus recovery from secondary effluent and side-stream liquid, which contain 5.9 and 68.2 mg/dm³ phosphorus, respectively. The phosphorus removal from side-stream liquid by using zirconium-loaded saponified orange waste (Zr-SOW) gel increased with an increasing solid/liquid ratio, and it was found that Zr-SOW gel showed better performance than zirconium ferrite. The prepared adsorbent was effective for phosphorus removal and exhibited a reasonably high adsorption capacity, twice than that of zirconium ferrite. The secondary effluent was treated in a column packed with Zr-SOW gel, and an dynamic adsorption capacity of 1.3 mol-P/kg was attained. The adsorbed phosphorus from the column was successfully eluted as a concentrated form by using a small amount of 0.2 M NaOH. Throughout the elution process, zirconium was not leaked from the adsorption gel.