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241.
Dacha Atienza Albert Calbet Enric Saiz Miquel Alcaraz Isabel Trepat 《Marine Biology》2006,150(2):221-235
In this work we studied the trophic ecology and feeding impact of the cladoceran Penilia avirostris and the cyclopoid copepod Oithona nana, the two dominant zooplankters in the summer communities of the coastal NW Mediterranean, on the naturally occurring microbial communities. In order to ascertain carbon surplus for growth and reproduction and the contribution to carbon and nitrogen recycling of these two species, we also determined their basal metabolism and excretion rates. The experiments conducted during summers 2002, 2003, and 2004 indicate that P. avirostris grazed mostly upon small flagellates, dinoflagellates, and diatoms, whereas O. nana had a narrower prey range, selecting motile organisms such as ciliates and occasionally dinoflagellates. The grazing impact of both species accounted, on average, for <10% of the standing stock of the microbial groups considered. In spite of the oligotrophic conditions, the feeding activity of P. avirostris is in general sufficient to compensate basal metabolism and allows a surplus for growth and reproduction. This was not the case for O. nana, its daily rations being often lower than the carbon basal demands. Regarding excretion rates, both species presented different N:P excretion ratios, the ones of O. nana falling within values typical for copepods, whereas the absence of detectable phosphorus excretion by P. avirostris implied an unbalance recycling with respect to typical Redfield ratio composition of marine seston. 相似文献
242.
Massively parallel sequencing has revolutionized our understanding of Mendelian disorders, and many novel genes have been discovered to cause disease phenotypes when mutant. At the same time, next-generation sequencing approaches have enabled non-invasive prenatal testing of free fetal DNA in maternal blood. However, little attention has been paid to using whole exome and genome sequencing strategies for gene identification in fetal disorders that are lethal in utero, because they can appear to be sporadic and Mendelian inheritance may be missed. We present challenges and advantages of applying next-generation sequencing approaches to gene discovery in fetal malformation phenotypes and review recent successful discovery approaches. We discuss the implication and significance of recessive inheritance and cross-species phenotyping in fetal lethal conditions. Whole exome sequencing can be used in individual families with undiagnosed lethal congenital anomaly syndromes to discover causal mutations, provided that prior to data analysis, the fetal phenotype can be correlated to a particular developmental pathway in embryogenesis. Cross-species phenotyping allows providing further evidence for causality of discovered variants in genes involved in those extremely rare phenotypes and will increase our knowledge about normal and abnormal human developmental processes. Ultimately, families will benefit from the option of early prenatal diagnosis. © 2014 John Wiley & Sons, Ltd. 相似文献
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Predation influences the ecology and behaviour of prey species and it is well known that the risk of predation affects prey’s decision making. We investigated whether predation risk through moon phase and exposure to the faecal odour of a natural predator, the red fox Vulpes vulpes, affect feeding behaviour and physiological response in wood mice (Apodemus sylvaticus). Antipredatory response was studied by live trapping under new and full moon in odourless control areas and areas experimentally manipulated with red fox fresh faeces. Food intake by individuals was determined as the amount of bait remaining in each trap and the physiological response was measured non-invasively analysing faecal corticosterone metabolites (FCM). Traps treated with faeces of red fox were the most avoided, and this avoidance was more significant during full moon. Food intake by wood mice varied according to the moon phase being significantly lower under full moon nights. We found sex, breeding condition and weight of individuals explaining the variation found in FCM concentrations, but no changes in FCM levels due to moon phase or exposure to red fox faeces were detected. These results indicate that wood mice avoid red fox faecal odour and this antipredatory response as well as feeding behaviour are significantly influenced by moon phase. However, no physiological response was found due to predation risk suggesting that wood mice do not take these predation cues enough reliable to experience physiological changes. 相似文献
247.
Niche complementarity, in which coexisting species use different forms of a resource, has been widely invoked to explain some of the most debated patterns in ecology, including maintenance of diversity and relationships between diversity and ecosystem function. However, classical models assume resource specialization in the form of distinct niches, which does not obviously apply to the broadly overlapping resource use in plant communities. Here we utilize an experimental framework based on competition theory to test whether plants partition resources via classical niche differentiation or via plasticity in resource use. We explore two alternatives: niche preemption, in which individuals respond to a superior competitor by switching to an alternative, less-used resource, and dominant plasticity, in which superior competitors exhibit high resource use plasticity and shift resource use depending on the competitive environment. We determined competitive ability by measuring growth responses with and without neighbors over a growing season and then used 15N tracer techniques to measure uptake of different nitrogen (N) forms in a field setting. We show that four alpine plant species of differing competitive abilities have statistically indistinguishable uptake patterns (nitrate > ammonium > glycine) in their fundamental niche (without competitors) but differ in whether they shift these uptake patterns in their realized niche (with competitors). Competitively superior species increased their uptake of the most available N form, ammonium, when in competition with the rarer, competitively inferior species. In contrast, the competitively inferior species did not alter its N uptake pattern in competition. The existence of plasticity in resource use among the dominant species provides a mechanism that helps to explain the manner by which plant species with broadly overlapping resource use might coexist. 相似文献
248.
A laboratory-based study was performed to assess the impact of climate warming on the recruitment of the endangered population
of the European lobster (Homarus gammarus) at Helgoland (North Sea, German Bight). Egg-bearing females collected in situ just after spawning in late summer were subjected
to various seasonal temperature regimes. Regimes with elevated temperatures (mild winters) resulted in a strong seasonal forward
shift of larval hatching. Hatching took place at significantly lower temperatures than under regimes with normal winters.
Experiments on larval development across a range of constant temperatures showed that no successful larval development occurred
at temperatures below 14°C. Larval survival increased from 9% at 14°C to 80% at 22°C, while duration of larval development
decreased correspondingly from 26 to 13 days. We hypothesize that an ongoing warming of the North Sea will strongly affect
the recruitment success of the Helgoland lobster, mainly resulting from a decoupling of the seasonal peak appearance of larvae
from optimal external conditions (temperature, food availability) for larval development. 相似文献
249.
Rosa M. Pintó M. Isabel Costafreda Francisco J. Pérez-Rodriguez Lucía D’Andrea Albert Bosch 《Food and environmental virology》2010,2(3):127-135
Hepatitis A is the most common among all hepatitis worldwide in spite of an efficient vaccine and improved hygiene. Shellfish-borne
outbreaks are still of major concern causing hundreds of cases and huge economical losses in the present context of global
food trade. Hepatitis A virus (HAV) is a unique picornavirus with many differences in its molecular biology including both
its incapacity to induce the inhibition of the cellular protein synthesis and a highly biased and deoptimized codon usage
with respect the cell. The final goal of this intriguing strategy seems to be the need for a fine-tuning control of the translation
kinetics, particularly at the capsid coding region, and the underlying mechanism is the use of a right combination of common
and rare codons to allow a regulated ribosome traffic rate thus ensuring the proper protein folding. Capsid folding is critical
to warrant a high environmental stability for a virus transmitted through the fecal–oral route with long extracorporeal periods. 相似文献
250.
<正>两个五年规划的过渡期是中国的关键时刻,受到中外分析家的密切关注。但30年的极速增长之后,这种发展模式的所有困境和后果也随之而来,而第十二个五年规划(以下简称"十二五"规划)展现了中国政府承诺将继续胸怀壮志地走向更可持续发展的道路模式。若"十二五"规划取得成功,"十二五"或将成为中国发展的关键时期,甚至将成为具有国 相似文献