Formic and acetic acid as agents for a cleaner fractionation of Miscanthus x giganteus

Miscanthus. x giganteus bark was subjected to acetic and formic acid fractionation. The kinetics of the process were explained by a model of two parallel first-order reactions, for pulp yield and delignification, and by Saeman's kinetics based models, for total polysaccharides and viscosity of pulps. The influence of hydrochloric acid concentration on both organosolv processes was studied at two levels: 0.10 and 0.15% by weight. The models provided a good fit of the experimental data and helped to predict the best set of independent variables to obtain a good fractionation.Both systems achieved similar delignification levels and pulp characteristics, although the evolution in formic acid was faster than in acetic acid, mainly due to the great extent of reactions in the warming up period that accounted for about a third part of the initial dry weight of the raw material.     

Active and legacy mining in an arid urban environment: challenges and perspectives for Copiapó, Northern Chile

Urban expansion in areas of active and legacy mining imposes a sustainability challenge, especially in arid environments where cities compete for resources with agriculture and industry. The city of Copiapó, with 150,000 inhabitants in the Atacama Desert, reflects this challenge. More than 30 abandoned tailings from legacy mining are scattered throughout its urban and peri-urban area, which include an active copper smelter. Despite the public concern generated by the mining-related pollution, no geochemical information is currently available for Copiapó, particularly for metal concentration in environmental solid phases. A geochemical screening of soils (n = 42), street dusts (n = 71) and tailings (n = 68) was conducted in November 2014 and April 2015. Organic matter, pH and elemental composition measurements were taken. Notably, copper in soils (60–2120 mg/kg) and street dusts (110–10,200 mg/kg) consistently exceeded international guidelines for residential and industrial use, while a lower proportion of samples exceeded international guidelines for arsenic, zinc and lead. Metal enrichment occurred in residential, industrial and agricultural areas near tailings and the copper smelter. This first screening of metal contamination sets the basis for future risk assessments toward defining knowledge-based policies and urban planning. Challenges include developing: (1) adequate intervention guideline values; (2) appropriate geochemical background levels for key metals; (3) urban planning that considers contaminated areas; (4) cost-effective control strategies for abandoned tailings in water-scarce areas; and (5) scenarios and technologies for tailings reprocessing. Assessing urban geochemical risks is a critical endeavor for areas where extreme events triggered by climate change are likely, as the mud flooding that impacted Copiapó in late March 2015.     

Functional clustering of varved lake sediment to reconstruct past seasonal climate

Annually laminated (varved) lake sediments constitutes excellent environmental archives, and have the potential to play an important role for understanding past seasonal climate with their inherent annual time resolution and within-year seasonal patterns. We propose to use functional data analysis methods to extract the relevant information with respect to climate reconstruction from the rich but complex information in the varves, including the shapes of the seasonal patterns, the varying varve thickness, and the non-linear sediment accumulation rates. In particular we analyze varved sediment from lake Kassjön in northern Sweden, covering the past 6400 years. The properties of each varve reflect to a large extent weather conditions and internal biological processes in the lake the year that the varve was deposited. Functional clustering is used to group the seasonal patterns into different types, that can be associated with different weather conditions. The seasonal patterns were described by penalized splines and clustered by the k-means algorithm, after alignment. The observed (within-year) variability in the data was used to determine the degree of smoothing for the penalized spline approximations. The resulting clusters and their time dynamics show great potential for seasonal climate interpretation, in particular for winter climate changes.     

Influence of the mineralogical composition on microbial activities in marine sediments: an experimental approach

We investigated the influence of the mineralogical composition of marine sediments on bacterial activity in experimental microcosms. Calcite and quartz were added to natural marine sediments and microbial response in terms of total bacterial abundance and biomass, β-D-glucosidase exo-enzymatic activity and bacterial incorporation of a radio-labelled (³H-leucine) substrate were investigated for a period of one month. We report here that after 15 days the mineralogical composition of the sediment (calcite vs. quartz) had an impact on bacterial abundance and activity (reduced for ca 15% and 56%, respectively). However, such impact was mitigated or even disappeared in high organic nutrient conditions.     

Herbivores on a dominant understory shrub increase local plant diversity in rain forest communities

Indirect effects of trophic interactions on biodiversity can be large and common, even in complex communities. Previous experiments with dominant understory Piper shrubs in a Costa Rican rain forest revealed that increases in herbivore densities on these shrubs caused widespread seedling mortality as a result of herbivores moving from Piper to seedlings of many different plant genera. We tested components of the Janzen-Connell hypothesis by conducting focused studies on the effects of specialist and generalist Piper herbivores on local seedling diversity. Whereas specialist herbivores are predicted to increase mortality to neighboring seedlings that are closely related to the source plant, true generalists moving from source plants may cause density-dependent mortality of many species, and possibly increase richness if new species replace abundant species that have been thinned by herbivores. Therefore, we hypothesized that seedling richness would be greater in understory control plots created in patches of Piper that had normal densities of generalist herbivores compared to plots from which we removed generalist herbivores manually from all Piper shrubs. After 15 months, generalist-herbivore-removal plots had > 40% fewer seedlings, > 40% fewer species, and 40% greater seedling evenness, on average, than control plots with generalist herbivores intact. Using a complementary approach in unmanipulated plots in four forests, we used path analysis to test for a positive association between seedling diversity and herbivore damage on Piper species. In unmanipulated plots, for both generalist and specialist herbivores, our data were significant fits to the causal model that Piper herbivores decrease evenness and increase plant species richness, corroborating the experimental results. Because herbivores changed how individuals were apportioned among the species and families present (lower evenness), one interpretation of these associations between herbivores on Piper shrubs and local seedling richness is that high seedling mortality in dominant families allowed the colonization or survival of less common species. If interspecific or apparent competition allowed for a relative increase in species richness, then the Janzen-Connell hypothesis may extend its predictions to generalist seedling predators. We speculate that apparent competition may explain some of the deviations from neutral model predictions, especially at small scales.     

Landowner Satisfaction with the Wetlands Reserve Program in Wisconsin

We evaluated ecological monitoring data and landowner perceptions to the federally funded Wetlands Reserve Program (WRP) in a three-county region in Wisconsin. We surveyed landowner satisfaction, involvement, participation, and use of the WRP restoration sites. We found that landowners are satisfied with the overall program (mean, 3.6 ± 0.2 [SE], on a scale of 1–5, with 5 being completely satisfied). WRP restorations significantly increased the area of wetland within the sites surveyed, the increase was primarily of fresh meadow (736.32 ha after restoration). Satisfaction is related to landowner participation during restoration and to the economic incentives provided by the WRP, Landowner satisfaction and the number of plant communities after restoration are unrelated to each other or to restoration and easement costs per hectare. Survey participants recommended some changes to the WRP, including a reduction in the tax rate of land enrolled in the WRP, approval for permanent deer stands, and increased communication with WRP officials during the restoration. Monitoring information collected for WRP restoration sites does not allow assessment of whether WRP sites are functionally equivalent to natural sites. We suggest that the WRP require a more rigorous monitoring program, including guidelines for invasive species control. Managers should also encourage collaborations with external researchers and consider restorations within an experimental framework.     

Effects of fish feeding by snorkellers on the density and size distribution of fishes in a Mediterranean marine protected area

Although there is a great deal of evidence to show that supplementary feeding by humans in terrestrial environments causes pronounced changes in the distribution and behaviour of wild animals, at present very little is known about the potential for such effects on marine fish. This study evaluated the consequences of feeding by snorkellers on fish assemblages in the no-take area of the Ustica Island marine protected area (MPA; western Mediterranean) by (1) determining if reef fish assemblage structure is affected in space and time by tourists feeding the fish; (2) assessing the effects of feeding on the abundance of the most common fish species; and (3) assessing the effects of feeding on the size structure of the two most numerically dominant ones. In particular, we hypothesised that both the abundance and the size structure of some fish species would increase at the study site following supplementary feeding, since the additional food provided by humans would make the site more appealing to them. Fish feeding influenced the fish assemblages within the Ustica MPA, and significant spatio-temporal changes occurred. While fish feeding appeared to have no effect on the ornate wrasse Thalassoma pavo, there was a noticeable increase in the number of Oblada melanura and Epinephelus marginatus in the impacted location after feeding. It is very likely that aggregations of fishes that evolve as a result of fish feeding by the public may have negative effects on local populations of fishes and invertebrates that make up their prey. Recreational use of coastal areas and MPAs is increasing elsewhere, making fish feeding a generalised human activity. Accurate information about its effect on the fish assemblage is essential to make responsible management decisions.Communicated by R. Cattaneo-Vietti, Genova     

Investigation of biomass waste biochar production to act as matrix for urea

Journal of Material Cycles and Waste Management - In this work, the synthesis of biochar from several biomass wastes to act as matrix for urea was investigated. The objective was to select the most...     

A Landscape Approach for Detecting and Evaluating Change in a Semi-Arid Environment

Vegetation change in the American West has been a subject of concern throughout the twentieth century. Although many of the changes have been recorded qualitatively through the use of comparative photography and historical reports, little quantitative information has been available on the regional or watershed scale. It is currently possible to measure change over large areas and determine trends in ecological and hydrological condition using advanced space-based technologies. Specifically, this process is being tested in a community-based watershed in southeast Arizona and northeast Sonora, Mexico using a system of landscape pattern measurements derived from satellite remote sensing, spatial statistics, process modeling, and geographic information systems technology. These technologies provide the basis for developing landscape composition and pattern indicators as sensitive measures of large-scale environmental change and thus may provide an effective and economical method for evaluating watershed condition related to disturbance from human and natural stresses. The project utilizes the database from the North American Landscape Characterization (NALC) project which incorporates triplicate Landsat Multi-Spectral Scanner (MSS) imagery from the early 1970s, mid 1980s, and the 1990s. Landscape composition and pattern metrics have been generated from digital land cover maps derived from the NALC images and compared across a nearly 20-year period. Results about changes in land cover for the study period indicate that extensive, highly connected grassland and desertscrub areas are the most vulnerable ecosystems to fragmentation and actual loss due to encroachment of xerophytic mesquite woodland. In the study period, grasslands and desertscrub not only decreased in extent but also became more fragmented. That is, the number of grassland and desertscrub patches increased and their average patch sizes decreased. In stark contrast, the mesquite woodland patches increased in size, number, and connectivity. These changes have important impact for the hydrology of the region, since the energy and water balance characteristics for these cover types are significantly different. The process demonstrates a simple procedure to document changes and determine ecosystem vulnerabilities through the use of change detection and indicator development, especially in regard to traditional degradation processes that have occurred throughout the western rangelands involving changes of vegetative cover and acceleration of water and wind erosion.     

Paternal factors in recurrent pregnancy loss: an insight through analysis of non-synonymous single-nucleotide polymorphism in human testis-specific chaperone HSPA2 gene

Environmental Science and Pollution Research - Heat shock protein A2 (HSPA2) is a testis-specific molecular chaperone of the 70 kDa heat shock protein (HSP70) family and reported to play a key role...