Evaluation of a Porcine Gastric Mucin and RNase A Assay for the Discrimination of Infectious and Non-infectious GI.1 and GII.4 Norovirus Following Thermal,Ethanol, or Levulinic Acid Plus Sodium Dodecyl Sulfate Treatments

Human noroviruses (NoVs) are a major source of foodborne illnesses worldwide. Since human NoVs cannot be cultured in vitro, methods that discriminate infectious from non-infectious NoVs are needed. The purpose of this study was to evaluate binding of NoV genotypes GI.1 and GII.4 to histo-blood group antigens expressed in porcine gastric mucin (PGM) as a surrogate for detecting infectious virus following thermal (99 °C/5 min), 70 % ethanol or 0.5 % levulinic acid (LV) plus 0.01 or 0.1 % sodium dodecyl sulfate (SDS) sanitizer treatments and to determine the limit of detection of GI.1 and GII.4 binding to PGM. Treated and control virus samples were applied to 96-well plates coated with 1 µg/ml PGM followed by RNase A (5 ng/µl) treatment for degradation of exposed RNA. Average log genome copies per ml (gc/ml) reductions and relative differences (RD) in quantification cycle (Cq) values after thermal treatment were 1.77/5.62 and 1.71/7.25 (RNase A) and 1.73/5.50 and 1.56/6.58 (no RNase A) for GI.1 and GII.4, respectively. Treatment of NoVs with 70 % EtOH resulted in 0.05/0.16 (GI.1) and 3.54/10.19 (GII.4) log reductions in gc/ml and average RD in Cq value, respectively. LV (0.5 %) combined with 0.1 % SDS provided a greater decrease of GI.1 and GII.4 NoVs with 8.97 and 8.13 average RD in Cq values obtained, respectively than 0.5 % LV/0.01 % SDS. Virus recovery after PGM binding was variable with GII.4 > GI.1. PGM binding is a promising surrogate for identifying infectious and non-infectious NoVs after capsid destruction, however, results vary depending on virus strain and inactivation method.     

More than just linking the nodes: civil society actors as intermediaries in the design and implementation of payments for ecosystem services–the case of a blue carbon project in Costa Rica

While in many cases the benefits of ecosystem services (ES) can be enjoyed on different scales, the provision of ES requires engagement and commitment at the local scale. Therefore, the local level becomes important when thinking about compensation schemes or payments for ecosystem services (PES) as an approach to managing ES. The difficult task of bringing together different actors and institutions at different scales for specific conservation projects at the local level often remains in the hands of intermediaries. In this paper, we investigate the role of a civil society organisation (CSO) as an intermediary organisation in a PES scheme, the Community Blue Carbon Project (CBCP) in Costa Rica. To assess the role of intermediaries in a PES scheme, we rely on social network analysis and examine the position and role of the intermediary organisation. Based on Net-Map interviews, which is an interview-based mapping tool that helps people understand, visualise, discuss and improve situations in which many different actors influence outcomes, we find that the intermediary organisation in the CBCP is composed of several institutional and individual intermediaries who create both formal networks for connecting the international to the local level and informal networks for creating trusting relationships among the actors. Different spatial levels are reflected within the CSO’s organisational structure, and the CSO mitigates the distributional, procedural, recognition and contextual aspects of environmental justice.     

Text analysis tools for identification of emerging topics and research gaps in conservation science

Keeping track of conceptual and methodological developments is a critical skill for research scientists, but this task is increasingly difficult due to the high rate of academic publication. As a crisis discipline, conservation science is particularly in need of tools that facilitate rapid yet insightful synthesis. We show how a common text‐mining method (latent Dirichlet allocation, or topic modeling) and statistical tests familiar to ecologists (cluster analysis, regression, and network analysis) can be used to investigate trends and identify potential research gaps in the scientific literature. We tested these methods on the literature on ecological surrogates and indicators. Analysis of topic popularity within this corpus showed a strong emphasis on monitoring and management of fragmented ecosystems, while analysis of research gaps suggested a greater role for genetic surrogates and indicators. Our results show that automated text analysis methods need to be used with care, but can provide information that is complementary to that given by systematic reviews and meta‐analyses, increasing scientists’ capacity for research synthesis.     

Effects of initial resources on the development of strains during a stressful training situation: Some counterintuitive results

Resource theories of occupational stress argue that employees' personal and environmental resources protect them from too much distress or strain during stressful work experiences. We examined four resources (emotional stability, previous experience, low drain on pre‐existing resources, and workgroup quality) available to soldiers at the beginning of a stressful 3‐month training experience as predictors of the trajectories of their strains over that period of time. Based on conservation of resources theory and the job demands–resources model, we predicted that the trends of strains would be more favorable (would increase more slowly or decline more quickly) if participants started the training with greater resources. The resources, primarily emotional stability and lack of pre‐existing resource drain, tended to be negatively related to strains, consistent with the idea that they can reduce strains. Significant interactions predicting trends were found predicting two of the three strains (post‐traumatic stress symptoms and depression, but not reports of physical health). Contrary to expectations, however, the three resources that significantly predicted trends over time (emotional stability, previous experience, and low pre‐existing resource drain) were associated with worsening rather than improving strains. Copyright © 2014 John Wiley & Sons, Ltd.     

The Influence of Two‐Stage Ditches with Constructed Floodplains on Water Column Nutrients and Sediments in Agricultural Streams

The two‐stage ditch is a novel management practice originally implemented to increase bank stability through floodplain restoration in channelized agricultural streams. To determine the effects of two‐stage construction on sediment and nutrient loads, we monitored turbidity, and also measured total suspended solids (TSS), dissolved inorganic nitrogen (N) species, and phosphorus (P) after two‐stage ditch construction in reference and manipulated reaches of four streams. Turbidity decreased during floodplain inundation at all sites, but TSS and P, soluble reactive phosphorus (SRP) and total phosphorus (TP) decreased only in the two‐stage ditches with longer duration of inundation. Both TSS and TP were positively correlated within individual streams, but neither were correlated with turbidity. Phosphorus was elevated in the stream to which manure was applied adjacent to the two‐stage reach, but not the reference reach, suggesting that landscape nutrient management plans could restrict nutrient transport to the stream, ultimately determining the efficacy of instream management practices. In addition, ammonium and nitrate decreased in two‐stage reaches with lower initial N concentrations. Overall, results suggest that turbidity, TSS, and TP were reduced during floodplain inundation, but the two‐stage alone may not be effective for managing high inorganic N loads.     

Ecology of drowning risk at a public swimming pool

INTRODUCTION: The fourth-leading cause of death in middle childhood is drowning, but there is remarkably little known about swimmer or lifeguard behavior patterns at public swimming pools. METHOD: This study used non-intrusive observational methodology to examine risk-taking by swimming patrons (predominantly children) and surveillance habits of lifeguards at a public swimming pool. The study also examined whether risk-taking behavior might be associated with density of swimmers, temperature, or lifeguard behaviors. RESULTS: Results suggested risk-taking behavior was common, with dangerous incidents observed over 90 times per hour. Particularly high were rates of running on the deck, which exceeded 100 incidents per hour near the deepest water of the pool, and jumping into the water dangerously close to other swimmers, which was witnessed about once every two minutes in the shallow water of the pool. Lifeguards tended to scan the pool well, and remain attentive to the areas under their responsibility, but they were distracted about 10 times per hour and warned patrons only about once for every 14 dangerous incidents observed. CONCLUSIONS: No consistent correlates to risk-taking behavior by swimming pool patrons were identified. Results are discussed with respect to previous findings and implications for intervention. Impact on Industry: Findings emphasize the need to increase awareness and adherence to safety rules by swimmers at swimming pools; to educate and remind lifeguards about proper swimming pool surveillance techniques; and to consider environmental changes at public swimming pools that might increase swimmer safety.     

A Data-Based Conservation Planning Tool for Florida Panthers

Habitat loss and fragmentation are the greatest threats to the endangered Florida panther (Puma concolor coryi). We developed a data-based habitat model and user-friendly interface so that land managers can objectively evaluate Florida panther habitat. We used a geographic information system (GIS) and the Mahalanobis distance statistic (D ²) to develop a model based on broad-scale landscape characteristics associated with panther home ranges. Variables in our model were Euclidean distance to natural land cover, road density, distance to major roads, human density, amount of natural land cover, amount of semi-natural land cover, amount of permanent or semi-permanent flooded area–open water, and a cost–distance variable. We then developed a Florida Panther Habitat Estimator tool, which automates and replicates the GIS processes used to apply the statistical habitat model. The estimator can be used by persons with moderate GIS skills to quantify effects of land-use changes on panther habitat at local and landscape scales. Example applications of the tool are presented.     

Decomposition of forest products buried in landfills

The objective of this study was to investigate the decomposition of selected wood and paper products in landfills. The decomposition of these products under anaerobic landfill conditions results in the generation of biogenic carbon dioxide and methane, while the un-decomposed portion represents a biogenic carbon sink. Information on the decomposition of these municipal waste components is used to estimate national methane emissions inventories, for attribution of carbon storage credits, and to assess the life-cycle greenhouse gas impacts of wood and paper products. Hardwood (HW), softwood (SW), plywood (PW), oriented strand board (OSB), particleboard (PB), medium-density fiberboard (MDF), newsprint (NP), corrugated container (CC) and copy paper (CP) were buried in landfills operated with leachate recirculation, and were excavated after approximately 1.5 and 2.5 yr. Samples were analyzed for cellulose (C), hemicellulose (H), lignin (L), volatile solids (VS), and organic carbon (OC). A holocellulose decomposition index (HOD) and carbon storage factor (CSF) were calculated to evaluate the extent of solids decomposition and carbon storage. Samples of OSB made from HW exhibited cellulose plus hemicellulose (C + H) loss of up to 38%, while loss for the other wood types was 0–10% in most samples. The C + H loss was up to 81%, 95% and 96% for NP, CP and CC, respectively. The CSFs for wood and paper samples ranged from 0.34 to 0.47 and 0.02 to 0.27 g OC g^?1 dry material, respectively. These results, in general, correlated well with an earlier laboratory-scale study, though NP and CC decomposition measured in this study were higher than previously reported.