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151.
The Cape fur seal, Arctocephalus pusillus pusillus is distributed along the southern African coastline from northern Namibia to the south-east coast of South Africa. The species
has been impacted by sealing operations since the 1600s, and historical records suggest that the taxon experienced a bottleneck
prior to the 20th century. Mitochondrial DNA control region sequences were generated for 106 individuals belonging to six
breeding colonies. Haplotype diversity was found to be high (0.975±0.014) whereas levels of nucleotide diversity were much
lower compared to other seal species (0.011±0.006). An analysis of molecular variance indicated that the largest percentage
of haplotype diversity is distributed within colonies rather than among them. This could be attributed to either extensive
gene flow among colonies, a lack of substantial female site philopatry, or incomplete lineage sorting of haplotypes. Mismatch
distribution and Fu’s F
S test indicated that the population has experienced a historical population expansion probably between c. 37,000–18,000 YBP and this date coincides very well with the height of the last glacial maximum when food resources were
abundant in the South Atlantic. These results also suggest that the recent sealing-induced bottleneck did not have a profound
influence on the haplotype diversity and a historical bottleneck prior to a demographic expansion may have been severe enough
to reduce nucleotide diversity substantially. 相似文献
152.
In order to characterize in more detail hemocytes from Mytilus edulis and establish links between histological and cytological featues, monoclonal antibodies (MABs) were prepared using hybridoma technology. Five MABs were identified and their reactivity patterns were analyzed with both immunoperoxidase and ultrastructural immunogold labelling. Four MAB classes were identified by their immunostaining patterns. Optical and ultrastructural examination revealed that Class I MAB reacted specifically with basophilic granulocytes (granulocytes containing small granules). Class II and III MABs differed in the intensity of their indirect immunofluorescence and degree of immunoperoxidase labelling, but all recognized both basophilic and eosinophilic granulocytes. Epitopes recognized by Class IV MABs were not always present in basophilic granulocytes, whereas they were always expressed in the eosinophilic granulocytes (granulocytes containing large granules) and could constitute differentiation antigens which progressively form during the maturation process. Molecular weights of the proteins recognized by these MABs were determined by Western blotting. It is concluded that immunostaining can identify at least three hemocyte types in M. edulis. 相似文献
153.
Comparative studies of fungal degradation of single or mixed bioaccessible reactive azo dyes 总被引:12,自引:0,他引:12
A screening using several fungi (Phanerochaete chrysosporium, Pleurotus ostreatus, Trametes versicolor and Aureobasidium pullulans) was performed on the degradation of syringol derivatives of azo dyes possessing either carboxylic or sulphonic groups, under optimized conditions previously established by us. T. versicolor showed the best biodegradation performance and its potential was confirmed by the degradation of differently substituted fungal bioaccessible dyes. Enzymatic assays (lignin peroxidase, manganese peroxidase, laccase, proteases and glyoxal oxidase) and GC-MS analysis were performed upon the assay obtained using the most degraded dye. The identification of hydroxylated metabolites allowed us to propose a possible metabolic pathway. Biodegradation assays using mixtures of these bioaccessible dyes were performed to evaluate the possibility of a fungal wastewater treatment for textile industries. 相似文献
154.
This study considers the characteristics of ground-level ozone (O3) in five Korean cities over a time period of 6-8 years. The focus of this study is daily maximum 1-hr and 8-hr concentrations. For all the study cities in the period examined, the mean and most of the percentiles (5, 10, 25, 50, 75, 90, and 95) for the daily maximum 1-hr and 8-hr concentrations showed increasing trends, although not all trends were statistically significant. The daily maximum 1-hr and 8-hr concentrations slowly increased during late winter, and peaks were attained during the summer season (from May to September). All the selected cities exhibited a high degree of correlation between their daily maximum 8-hr and 1-hr concentrations. The daily maximum 8-hr concentrations, which were climatologically equivalent to the Korean 1 hr/100 parts per billion (ppb) standard, were higher than the current 8 hr/60 ppb by a difference of 8-16 ppb. Compared with other cities in Korea, Seoul recorded a substantially higher frequency of days and hours with concentrations above 1 hr/100 ppb, and a higher frequency of days with concentrations above 8 hr/60 ppb and 8 hr/80 ppb. Seoul also recorded a substantially higher frequency of hours with concentrations above 1 hr/100 ppb than days with concentrations above 1 hr/100 ppb, implying that on some days severe exceedances persisted for more than one hour per day. During multiple-day episodes a North Pacific High dominated Korea, which is quite typical in Korea during the summer season. 相似文献
155.
Christoffoleti PJ Trentin R Tocchetto S Marochi A Galli AJ López-Ovejero RF Nicolai M 《Journal of environmental science and health. Part. B》2005,40(1):59-67
During the growing season of 2002--2003, field and greenhouse experiments were conducted with the objective of evaluating the influence of Italian ryegrass phenological stages and management alternatives on the control of resistant biotypes to glyphosate. Three field experiments were conducted in Lagoa Vermelha, RS, Brazil and glyphosate was applied alone and in combinations with alternative herbicides. Two greenhouse experiments were also conducted at the Department of Crop Science, ESALQ/USP, Piracicaba, SP, Brazil. The Italian ryegrass resistant population was collected from Lagoa Vermelha, RS, Brazil. From the results it was possible to conclude that: (i) the more advanced the phenological stage of application, the more difficult the control of resistant Italian ryegrass by glyphosate, mainly by the rate of 960 g a.i. ha(-1); however, this rate applied at earlier phenological stage (five tillers), the control was higher than 90%; (ii) with the increment of glyphosate rate, it significant response was observed on the control at all stages of application; (iii) the mixture of glyphosate + clethodim (1440 + 72 g a.i. ha(-1)), paraquat + diuron (500 + 250 g a.i. ha(-1)), at all stages of application and clethodim (96 g a.i. ha(-1)) and paraquat + diuron (300 + 150 g a.i. ha(-1)) at the initial stages until pre-flowering were excellent alternatives for management of these populations; and (iv) the response of control was much faster for the mixture of glyphosate + clethodim, independently of growth stage. 相似文献
156.
A limited number of different foods were analysed for dioxin-like compounds by the CALUX bioassay which is an in vitro luciferase reporter gene assay measuring chemical activation of the aryl hydrocarbon receptor. Sixty-two milk samples were obtained from a surveillance campaign, 34 meat samples and 34 fishery products were purchased from the Belgian market. Bio-analytical and chemo-analytical dioxin toxicity equivalents (TEQ) values of the same milk samples were compared. Spearman's Rank correlation coefficients of 0.72, 0.67, 0.73 were obtained respectively between CALUX-TEQ and PCDD/F-TEQ, DL-PCB-TEQ and PCDD/F+DL-PCB-TEQ. The bioassay limit of detection was 0.1 pg TEQ from 1 g animal lipid, the limit of quantification was 0.4 pg TEQ. The repeatability of the CALUX bioassay (variability of butter fat samples analysed in the same run) showed a coefficient of variation (CV) of 10%, intra laboratory reproducibility based on independent runs of the same butter fat samples showed more variation (CV of 26% for samples above 2 pg TEQ/g lipid). All milk samples with a chemical TEQ value above the current limit value in Belgium showed an elevated CALUX-TEQ concentration, above 6 pg TEQ/g lipid. No false negative results were obtained. Based on the good correlation between CALUX-TEQ and chemically measured TEQ levels, the CALUX bioassay can be recommended as a screening tool for routine measurement of potentially toxic PHAHs in milk samples. Chemical analyses could then largely be restricted to positive samples, in order to identify the nature and to quantify the concentration of the chemicals that give the positive signal. Meat samples showed lower CALUX-TEQ values per gram lipid compared to fish samples. The fish samples showed a wider range of CALUX-TEQ values than the meat samples. 相似文献
157.
Dewulf J van Langenhove H 《Environmental science and pollution research international》2002,9(4):267-273
Life cycle analysis is one of the tools in the assessment of the sustainability of technological options. It takes into account all effects on the ecosystem and the population which may endanger the possibilities of current and future generations. However, the main bottleneck in current LCA methodologies is the balancing of different effects, being all quantified on different scales. In this work, a methodology is proposed, which allows one to quantify different effects of the production, consumption and disposal of goods, and services on a single scale. The basis of the methodology is the second law of thermodynamics. All production, consumption and disposal processes affecting the ecosystem and the population, are quantified in terms of loss of exergy. The exergy content of a material is the maximum amount of energy which can be transformed into work at given environmental conditions. Next to the elaboration of the methodology, the new approach is illustrated by examples of the production of synthetic organic polymers, inorganic building insulation materials and different waste gas treatment options. 相似文献
158.
Désy JC Amyot M Pinel-Alloul B Campbell PG 《Environmental pollution (Barking, Essex : 1987)》2002,120(3):759-769
We found that Cd concentrations in three species of macrophyte-associated invertebrates (the gastropods Bithynia tentaculata and Physa gyrina, and the amphipod Gammarus fasciatus) collected at twenty sites along the St. Lawrence River were correlated with Cd concentrations in their main food source, i.e. macrophytes and associated periphyton. Cd in these invertebrates was not significantly correlated with Cd concentrations in the sediments (even when corrections for iron oxide or organic carbon content were applied) or to calculated free-cadmium concentrations in the water. Cd levels in juveniles of B. tentaculata were very tightly linked to Cd concentrations in macrophytes, which is consistent with the close relationship that these organisms have developed with macrophytes. Linear models predicting Cd levels in juveniles of B. tentaculata from Cd levels in different macrophyte species were strong (R2 = 0.69-0.90). Analysis of covariance on these models showed no statistical difference of slope or intercept for any of the macrophyte species, except Vallisneria americana. We suggest that the macrophyte-periphyton complex is a key link in the transfer of cadmium to some aquatic invertebrates in the littoral zone of the St. Lawrence River and that macrophytes and their associated epiphytes should also be used as biomonitors. 相似文献
159.
Jo Wheeler 《生态毒理学报》2004,(3):26-27
毫不令人吃惊,通过保护濒危物种,诸如老虎、犀牛和大熊猫,全世界大多数人都与世界野生动物基金会(WWF)和著名的大熊猫标志有联系.然而,为什么WWF会对人类在地球上的住宅感兴趣呢?…… 相似文献
160.
Jonathan Mark Wilson Alexandre Leitão Ana Filipa Gonçalves Catarina Ferreira Patrick Reis-Santos Ana-Violeta Fonseca Joana Moreira da Silva José Carlos Antunes Cristina Pereira-Wilson João Coimbra 《Marine Biology》2007,151(5):1633-1645
The Anguillid juvenile glass eel must deal with the osmoregulatory consequences of highly variable environmental salinities
on its recruitment migration from coastal to fresh waters. Changes in ionoregulatory parameters and branchial ion transport
protein [Na+/K+-ATPase, Na+:K+:2Cl− cotransporter (NKCC), cystic fibrosis transmembrane regulator (CFTR) anion channel, V-type proton ATPase] expression (activities,
protein and/or mRNA level expression and/or cellular localization) in response to acclimation to a broad range of ionic strengths
[distilled water (DW) to hypersaline water (HSW; 150%) sea water (SW 32‰)] was studied. The estuarine glass eels were very
euryhaline and successfully acclimated to acute changes in environmental ionic strength from 50% SW, with high mortality only
observed in HSW (51%) and sublethal osmoregulatory indicators (whole body water content and sodium levels) disturbed at the
extremes (DW and HSW). Central to a high salinity acclimation were elevated branchial Na+/K+-ATPase, NKCC and CFTR expression. At lower salinity, Na+/K+-ATPase expression was maintained and NKCC and CFTR expressions were reduced. Branchial chloride cells increased in size up
to SW but decreased in HSW. During hypotonic disturbance (DW), no compensatory elevation in V-ATPase or Na+/K+-ATPase expression was observed. 相似文献