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891.
Using the polymerase chain reaction (PCR), it was possible to amplify a single copy fragment of the β-globin gene from 2–32 human embryonic cells obtained from arrested preimplantation embryos. For the detection of β-thalassaemia mutations, allele specific priming of the PCR using nested primers was employed using approximately 10 pg of DN A from individuals known to carry these mutations. This approach was successful in detecting the presence or absence of five Asian Indian β-thalassaemia mutations that were selected for this study. In spite of meticulous precautions against contamination, false-positive amplification was observed, a problem that will have to be overcome before this approach can be used in clinical practice. 相似文献
892.
A framework is presented showing the process of integrated resource planning in the electric power sector. This takes into account the traditional utility planning process, and shows how the use of advanced decision analysis tools would facilitate the integration of demand-side management (DSM) and environmental factors. The concept of influence diagram is introduced in the utility planning arena. Using the interdependent data analysis (IDA) technique, a way is shown to provide the probability estimates necessary for the influence diagram. The IDA technique allows the use of expert opinions and intuitive judgements to develop the necessary probability estimates. A sample case study is presented where the issue of environmental impact from stack emissions is incorporated. A base case coal option is compared against a gas turbine combined cycle (GTCC) alternative. Energy costs from these two options are compared by taking into account the capital, licensing, fuel, operation and maintenance, and emissions related costs. 相似文献
893.
E. Machevin-Surugue N. David E. Verspyck G. Labadie G. Blaysat I. Durand V. Ickowicz L. Marpeau 《黑龙江环境通报》2002,22(10):898-902
The dilated coronary sinus (DCS) has only recently been clearly visualised in the fetus due to progress in prenatal echography. This is a retrospective study of 22 fetuses presenting with DCS revealed by prenatal echography. We report the circumstances leading to the detection of a DCS and the neonatal outcome of these fetuses. The coronary sinus was defined as dilated depending on its visualisation in cross-section from the ‘4 chamber’ view, as well as a pseudo inter-atrial septal defect from a more posterior view. In each case the gestational age, circumstances of detection, associated anomalies and postnatal outcome are reported. The circumstances were: evaluation of a clearly identified DCS in four cases and during detailed fetal echocardiography because of suspected congenital heart disease in 18 cases. Five cases were associated with a cardiac anomaly, three with an extracardiac anomaly, six with both cardiac and extracardiac anomaly and eight were isolated. Postnatal outcome was related to the associated anomaly. In conclusion, it is important that the echography image be correctly interpreted, as a DCS often implies possible associated defects and therefore affects prognosis. When not associated with other anomalies this condition is not considered serious. Copyright © 2002 John Wiley & Sons, Ltd. 相似文献
894.
Congenital limb deficiency (CLD) occurs in 0.54 to 0.59/1000 live-born infants and varies according to its anatomic location, type and cause. Our aim was to present a fetus with a prenatal ultrasonographic diagnosis of CLD (transverse reduction deficiency of the left upper limb), and to determine the prevalence and clinical characteristics of CLD at a tertiary medical centre in Israel. Among 78 500 live-born infants, there were 24 cases of CLD (0.31/1000): 45.8% of the CLD cases with affected upper limbs, 45.8% with affected lower limbs, and 8.4% with both limbs affected; 88.4% of the limb deficiencies were longitudinal and 11.6% were transverse; and, 33.3% (8/24) of the affected newborn infants had additional congenital anomalies. We conclude that CLD is not an infrequent finding in live-born infants. Comprehensive ultrasonography of the fetus allows early prenatal diagnosis of CLD and provides the parents with important information and helps them in their decision regarding the fate of the pregnancy. Copyright © 2003 John Wiley & Sons, Ltd. 相似文献
895.
The acute toxicity of two organophosphorus (OP) insecticides, azinphosmethyl and acephate, was evaluated in the mummichog, Fundulus heteroclitus. In addition, the effects of certain intrinsic (sex) and extrinsic (salinity and multiple toxicant interraction) variables on the toxic response were also investigated. Azinphosmethyl was by far the most toxic of the two OP insecticides with a 96h LC50 approximately 100,000 x lower than that for acephate. Slight sex differences were observed in the sensitivity of mummichogs to each of the OP insecticides with male fish being marginally more sensitive. Significant effects of low salinity stress were observed only with acephate exposure and, in this case, low salinity appeared to be slightly protective. In general, all of the insecticide mixtures (azinphosmethyl/endosulfan, azinphosmethyl/fenvalerate and acephate/fenvalerate) tested exhibited simple additive toxicity. 相似文献
896.
Aerosol composition as a function of haze and humidity levels in the Southwestern U.S. 总被引:1,自引:0,他引:1
Luis A. de P. Vasconcelos Edward S. Macias Warren H. White 《Atmospheric environment (Oxford, England : 1994)》1994,28(22)
The observed composition of visibility-reducing aerosols in the Grand Canyon region is summarized in climatological terms. Observations are from SCENES, a measurement program extending from 1984 to 1989. Results are presented as average mass balances stratified by various factors.Aerosols were found to exhibit substantial seasonal variation, but little systematic diurnal variation. Crustal material was a dynamic component, and peaked during springtime. Aerosol composition, but not total concentration, depended strongly on ambient relative humidity, with crustal material augmented at low humidities and sulfates augmented at high humidities. Total fine-particle concentrations correlated strongly with light scattering, as expected; however, little association between chemical composition and light scattering was observed. 相似文献
897.
898.
An easy and reproducible technique for direct fetal chromosome analysis after chorionic biopsy is described. Very high colchicine concentration and rehydratation of the fixed villi are the two original points of this method. 相似文献
899.
Dr Isabel Lorda-Sánchez Dan Diego-Alvarez Carmen Ayuso Marta Rodríguez de Alba Maria Jose Trujillo Carmen Ramos 《黑龙江环境通报》2005,25(10):934-938
Balanced reciprocal translocation is one of the known causes of recurrent spontaneous abortions. Cytogenetic studies of unbalanced miscarriages are difficult due to the growth failure of early loss and usually macerated abortions. We present a molecular study of an abortion in which the father carries a balanced reciprocal translocation t(2;17)(q32.1;q24.3) using QF-PCR and CGH techniques. DNA analysis showed the presence of a trisomy 2 due to a 3:1 interchange segregation. Recombinant events could also be investigated by comparing DNA samples from the family. We propose QF-PCR in addition to CGH as an efficient diagnostic method to improve our knowledge of unbalanced offspring in balanced translocation carriers. Copyright © 2005 John Wiley & Sons, Ltd. 相似文献