Mole rats (<Emphasis Type="Italic">Spalax ehrenbergi</Emphasis>) select bypass burrowing strategies in accordance with obstacle size

Mole rats inhabit extensive individual tunnel systems. Since the energetic cost of burrowing far exceeds that of surface locomotion, excellent orientation ability is crucial. Here we examined whether mole rats can bypass an obstacle (ditch) intersecting an existing tunnel in order to rejoin the two tunnel sections. The mole rats dug two bypass types, depending on the size of the obstacle confronting them: a bypass around the small ditches, parallel and close to the ditch walls; or a bypass beneath the floor of the large ditches. These results demonstrate that the mole rat has the ability to avoid obstacles by digging accurate and energy-conserving bypass tunnels. In order to utilize such a capacity, the mole rat must possess both the means to evaluate the size of the obstacle as well as the ability to perceive its exact position relative to the original tunnel that it will rejoin. Possible mechanisms of orientation that could explain such ability are briefly discussed.     

Evidence of behavioral co-option from context-dependent variation in mandible use in trap-jaw ants (Odontomachus spp.)

Evolutionary co-option of existing structures for new functions is a powerful yet understudied mechanism for generating novelty. Trap-jaw ants of the predatory genus Odontomachus are capable of some of the fastest self-propelled appendage movements ever recorded; their devastating strikes are not only used to disable and capture prey, but produce enough force to launch the ants into the air. We tested four Odontomachus species in a variety of behavioral contexts to examine if their mandibles have been co-opted for an escape mechanism through ballistic propulsion. We found that nest proximity makes no difference in interactions with prey, but that prey size has a strong influence on the suite of behaviors employed by the ants. In trials involving a potential threat (another trap-jaw ant species), vertical jumps were significantly more common in ants acting as intruders than in residents (i.e. a dangerous context), while horizontal jumps occurred at the same rate in both contexts. Additionally, horizontal jump trajectories were heavily influenced by the angle at which the substrate was struck and appear to be under little control by the ant. We conclude that while horizontal jumps may be accidental side-effects of strikes against hard surfaces, vertical escape jumps are likely intentional defensive behaviors that have been co-opted from the original prey-gathering and food-processing functions of Odontomachus jaws.     

Fate of PCBs, PAHs and their source characteristic ratios during composting and digestion of source-separated organic waste in full-scale plants

Composting and digestion are important waste management strategies. However, the resulting products can contain significant amounts of organic pollutants such as polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs). In this study we followed the concentration changes of PCBs and PAHs during composting and digestion on field-scale for the first time. Concentrations of low-chlorinated PCBs increased during composting (about 30%), whereas a slight decrease was observed for the higher chlorinated congeners (about 10%). Enantiomeric fractions of atropisomeric PCBs were essentially racemic and stable over time. Levels of low-molecular-weight PAHs declined during composting (50-90% reduction), whereas high-molecular-weight compounds were stable. The PCBs and PAHs concentrations did not seem to vary during digestion. Source apportionment by applying characteristic PAH ratios and molecular markers in input material did not give any clear results. Some of these parameters changed considerably during composting. Hence, their diagnostic potential for finished compost must be questioned.     

Prenatal diagnosis of thrombocytopenia absent radius syndrome using ultrasound and fetoscopy

The prenatal diagnosis of thrombocytopenia absent radius syndrome in both dizygotic twins utilizing ultrasound, radiography and fetoscopy is described.     

Rapid Geomorphic and Habitat Stream Assessment Techniques Inform Restoration Differently Based on Levels of Stream Disturbance

Visual‐based rapid assessment techniques provide an efficient method for characterizing the restoration potential of streams, with many focusing on channel stability and instream habitat features. Few studies, however, have compared these techniques to see if they result in differing restoration priorities. Three rapid assessment techniques were contrasted at three wild trout streams in western New York with different amounts of channel disturbance. Two methods focused only on geomorphic stability, whereas the third addressed physical habitat condition. Habitat assessment scores were not correlated with scores for either geomorphic assessment method and they varied more between channels with different degrees of disturbance. A model based on dynamic equilibrium concepts best explains the variation among the streams and techniques because it accounts for a stream's capacity to maintain ecological integrity despite some inherent instability. Geomorphic indices can serve as effective proxies for biological indices in highly disturbed systems. Yet, this may not be the case in less disturbed systems, where geomorphic indices cannot differentiate channel adjustments that impact biota from those that do not. Dynamically stable streams can include both stable and unstable reaches locally as characterized by geomorphic methods and translating these results into restoration priorities may not be appropriate if interpretations are limited to the reach scale.     

Effects of Land Use and Climate Change on Stream Temperature II: Threshold Exceedance Duration Projections for Freshwater Mussels

We developed a stochastic hourly stream temperature model (SHSTM) to estimate probability of exceeding given threshold temperature (T) for specified durations (24 and 96 h) to assess potential impacts on freshwater mussels in the upper Tar River, North Carolina. Simulated daily mean stream T from climate change (CC) and land‐use (LU) change simulations for 2021‐2030 and 2051‐2060 were used as input to the SHSTM. Stream T observations in 2010 revealed only two sites with T above 30°C for >24 h and Ts were never >31°C for more than 24 h at any site. The SHSTM suggests that the probability, P, that T will exceed 32°C for at least 96 h in a given year increased from P = 0, in the 20th Century, to P = 0.05 in 2021‐2030 and to P = 0.14 in 2051‐2060. The SHSTM indicated that CC had greater effects on P for 24 and 96 h durations than LU change. Increased P occurred primarily in higher order stream segments in the downstream reaches of the basin. The SHSTM indicated that hourly stream T responded to LU change on the daily scale and did not affect stream T for durations >24 h. The SHSTM indicated that known thermal thresholds for freshwater mussels could be exceeded within the next 50 years in many parts of the upper Tar River basin in North Carolina, which could have negative consequences on the recruitment of freshwater mussels.     

Bedded Sediment Conditions and Macroinvertebrate Responses in New Mexico Streams: A First Step in Establishing Sediment Criteria

Excess fine sediments in streambeds are among the most pervasive causes of degradation in streams of the United States. Simple criteria for acceptable streambed fines are elusive because streambed fines and biotic tolerances vary widely in the absence of human disturbances. In response to the need for sediment benchmarks that are protective of minimum aquatic life uses under the Clean Water Act, we undertook a case study using surveys of sediment, physical habitat, and macroinvertebrates from New Mexico streams. Our approach uses weight of evidence to develop suggested benchmarks for protective levels of surficial bedded sediments <0.06 mm (silt and finer) and <2.0 mm (sand and finer). We grouped streams into three ecoregions that were expected to produce similar naturally occurring streambed textures and patterns of response to human disturbances. Within ecoregions, we employed stressor response models to estimate fine sediment percentages and bed stability that are tolerated by resident macroinvertebrates. We then compared individual stream sediment data with distributions among least‐disturbed reference sites to determine deviation from natural conditions, accounting for natural variability across ecoregion, gradient, and drainage area. This approach for developing benchmark values could be applied more widely to provide a solid basis for developing bedded sediment criteria and other protective management strategies in other regions.     

Organochlorine contamination in shorebirds from Washington state and the significance for their falcon predators

Arctic-breeding shorebirds collected in western Washington state during winter and spring, and a comparative sample collected in coastal California during the winter were analyzed for organochlorine contaminants to determine the potential impact of these residues on populations of peregrine falcons (Falco peregrinus) and merlins (F. columbarius) which prey upon shorebirds in western Washington. Dunlins (Calidris alpina), an important winter prey for falcons in western Washington, were collected between 1975 and 1981. During winter 1980–81, dunlins carried low organochlorine residues; DDE levels ranged from 0.01 to 1.2 ppm, and PCB levels ranged from 0.02 to 0.82 ppm (wet weight). Levels of other organochlorine contaminants (HCB, Chlordane compounds, Dieldrin, and Heptachlor Epoxide), analyzed in a subsample of dunlins, were consistently lower than DDE and PCB levels, and ranged from 0.001 to 0.22 ppm (wet weight). Dunlins in western Washington did not significantly increase their DDE or PCB burdens over the 1980–81 winter. A decline in DDE residues between 1978 and 1981 was noted, and declines in PCB residues from both 1975 and 1978 to 1980–81 were noted. Residues in other wintering shorebirds from western Washington were similar. Wintering sanderlings (Calidris alba) from California, revealed much higher DDE contamination than in Washington (up to 32 ppm, wet weight). Spring migrant shorebirds in western Washington contained both low and very high DDE residues (up to 417 ppm, wet weight). There is evidence suggesting these high DDE concentrations are accumulated along the Pacific coast of North America.     

Mitochondrial energetics of benthic and pelagic Antarctic teleosts

Antarctic fauna are highly adapted to the frigid waters of the Southern Ocean. This study describes the in vitro temperature sensitivity of oxygen consumption rates measured in liver mitochondria from the pelagic notothenioid Pleuragramma antarcticum between 5 and 35 °C. Oxygen fluxes were measured after the addition of millimolar levels of pyruvate, malate, succinate and glutamate (state II, LEAK) and saturating levels of ADP [state III, oxidative phosphorylation (OXPHOS)]. State III respiration significantly decreased above 18.7 °C. A comparison of the oxidative capacities among P. antarcticum and other notothenioids showed significant differences in state III respiration, where benthic species exhibited about 50 % lower rates than P. antarcticum. In addition, state III respiration rates normalized per milligram of mitochondrial protein of P. antarcticum were up to eight times higher than state III rates reported in the literature for other notothenioids. The comparatively high respiration rates measured in this study may be explained by our approach, which engaged both complexes I and II under conditions of oxidative phosphorylation. State III rates of independently activated complexes I and II were found to range from 42 to 100 % of rates obtained when both complexes were activated simultaneously in the same species. The remarkable tolerance of P. antarcticum OXPHOS toward warmer temperatures was unexpected for an Antarctic stenotherm and may indicate that thermal sensitivity of their mitochondria is not the driving force behind their stenothermy.