Use of a physiologically based extraction test to estimate the human bioaccessibility of potentially toxic elements in urban soils from the city of Glasgow,UK

A simple, two-stage, physiologically based extraction has been applied to assess the human bioaccessibility of potentially toxic elements (PTE) in 20 urban soils from a major UK city. Chromium and iron bioaccessibilities were found to be markedly higher in the intestinal phase, whilst lead and zinc bioaccessibilities were higher in the stomach. Copper and manganese bioaccessibilities were generally similar under both extraction conditions. Principal component analysis was used to study relationships amongst bioaccessible element concentrations and land use. Distinctions could be observed between the distributions of the urban metals—copper, lead and zinc—and metals predominantly of geogenic origin, such as iron. There was no clear delineation between roadside soils and soils obtained from public parks. Bioaccessible analyte concentrations were found to be correlated with pseudototal (aqua regia soluble) analyte concentrations for all elements except iron. Results of the BCR sequential extraction did not, in general, provide a good indication of human bioaccessibility. Comparison of bioaccessible PTE concentrations with toxicological data indicated that lead is the element of greatest concern in these soils but that levels are unlikely to pose a health risk to children with average soil intake.     

Examining the Factors Affecting Air Pollution Emission Growth in China

In this study, a structural decomposition method was applied to research the factors affecting the changes in air pollution emissions in China. Based on 1995–2009 data from the World IO Database, we combine China’s (Import) Noncompetitive IO Table and the Environmental Account Table. The results indicate that emission intensities represent the most important factor for reducing air pollution emissions in China. In contrast, economies of scale and the intermediate input product structure constitute the major causes for acceleration in the growth of air pollution emissions in China. From the perspective of final demand, the economic scale effect caused by investment demand is the main reason for this accelerated growth in China’s air pollution emissions in recent years. Consumption-driven economic growth is cleaner, while investment-driven economic growth is dirtier. This study constructed a structure decomposition model based on the input-output tables, which is suitable for studying the driving forces of various economic indicators, such as energy, carbon dioxide, and economic growth. At the same time, this method is helpful for analyzing the factors that influence changes in economic indicators that result from different economic pull modes, such as the final demand mode. However, the model does have limitations; for example, it does not consider the difference between general trade and processing trade in exports.     

A neural network experiment on the simulation of daily nitrate-nitrogen and suspended sediment fluxes from a small agricultural catchment

In this paper, we report an application of neural networks to simulate daily nitrate-nitrogen and suspended sediment fluxes from a small 7.1 km² agricultural catchment (Melarchez), 70 km east of Paris, France. Nitrate-nitrogen and sediment losses are only a few possible consequences of soil erosion and biochemical applications associated to human activities such as intensive agriculture. Stacked multilayer perceptrons models (MLPs) like the ones explored here are based on commonly available inputs and yet are reasonably accurate considering their simplicity and ease of implementation. Note that the simulation does not resort on water quality flux observations at previous time steps as model inputs, which would be appropriate, for example, to predict the water chemistry of a drinking water plant a few time steps ahead. The water quality fluxes are strictly mapped to historical mean flux values and to hydro-climatic variables such as stream flow, rainfall, and soil moisture index (12 model input candidates in total), allowing its usage even when no flux observations are available. Self-organizing feature maps based on the network structure established by Kohonen were employed first to produce the training and the testing data sets, with the intent to produce statistically close subsets so that any difference in model performance between validation and testing has to be associated to the model and not to the data subsets. The stacked MLPs reached different levels of performance simulating the nitrate-nitrogen flux and the suspended sediment flux. In the first instance, 2-input stacked MLP nitrate-nitrogen simulations, based on the same-day stream flow and on the 80-cm soil moisture index, have a performance of almost 90% according to the efficiency index. On the other hand, the performance of 3-input stacked MLPs (same-day stream flow, same-day historical flux, and same-day stream flow increment) reached a little more than 75% according to the same criterion. The results presented here are deemed already promising enough, and should encourage water resources managers to implement simple models whenever appropriate.     

Fate of metal-associated POM in a soil under arable land use contaminated by metallurgical fallout in northern France

Organic matter is a major metal-retaining constituent in soils. Among the diversity of organic components in soils, particulate organic matter (POM) accumulates large amounts of metals, but the fate of such metal-associated POM is unknown. We studied different POM size fractions and their corresponding mineral size-fractions isolated from the surface horizon of a soil affected by metallurgical fallout. Analyses of total and EDTA extractible metal contents performed on all size fractions demonstrated that with decreasing POM size, larger metal concentrations were observed but they were less extractable. Micromorphological study revealed the occurrence of opaque parts in decaying POM fragments and their individualization as fine, irregularly shaped opaque fragments in the soil matrix. This work suggested a mutual sequestration of metal pollutants and organic carbon as micro-meter sized, metal-enriched organic particles derived from POM, representing an original pathway for natural attenuation of risk related to metal contaminated soils.     

Effects of meteorological conditions and topography on the bioaccumulation of PAHs and metal elements by native lichen (Xanthoria parietina)

The bioaccumulation of PAHs and metal elements in the indigenous lichens Xanthoria parietina was monitored during two years at a quarterly frequency, in 3 sites of contrasted anthropic influence. The impact of the meteorological factors (temperature, relative humidity, rainfall, wind speed) was first estimated through principal component analysis, and then by stepwise multilinear regressions to include wind directions. The pollutants levels reflected the proximity of atmospheric emissions, in particular from a large industrial harbor. High humidity and mild temperatures, and in a lower extent low wind speed and rainfall, also favored higher concentration levels. The contributions of these meteorological aspects became minor when including wind direction, especially when approaching major emission sources. The bioaccumulation integration time towards meteorological variations was on a seasonal basis (1–2 months) but the wind direction and thus local emissions also relied on a longer time scale (12 months). This showed that the contribution of meteorological conditions may be prevalent in remote places, while secondary in polluted areas, and should be definitely taken into account regarding long-term lichen biomonitoring and inter-annual comparisons. In the same time, a quadruple sampling in each site revealed a high homogeneity among supporting tree species and topography. The resulting uncertainty, including sampling, preparation and analysis was below 30% when comfortable analytical conditions were achieved. Finally, the occurrence of unexpected events such as a major forest fire, permitted to evaluate that this type of short, although intense, events did not have a strong influence on PAH and metals bioaccumulation by lichen.     

Do herbicide treatments reduce the sensitivity of mosquito larvae to insecticides?

Invasive mosquitoes are economic and sanitary concerns especially in Europe and America. Most work has emphasized the role of resistance [Berrada, S., Fournier, D., Cuany, A., Nguyen, T.X., 1994. Identification of resistance mechanisms in a selected laboratory strain of Cacopsylla pyri (Homoptera: Psyllidae): altered acetylcholinesterases and detoxifying oxidases. Pesticide Biochemistry and Physiology 48, 41-47; Hemingway, J., Hawkes, N.J., McCarroll, L., Ranson, H., 2004. The molecular basis of insecticide resistance in mosquitoes. Insect Biochemistry and Molecular Biology 34, 653-665] to insecticides. Compounds acting on larval sensitivity to insecticides are not well studied and their action remains poorly understood. Among several residual chemicals in ecosystems, particularly in wetlands, we identified a possible interaction of an herbicide on larval resistance to an insecticide. Our work contributes to the global control of mosquito populations by identifying possible pathways of resistance to insecticides of these vectors. Resistance or tolerance to insecticide treatments might contribute to successful invasion by mosquitoes. Here we report an ecotoxicological approach to test the hypothesis of an indirect effect of atrazine on mortality of an invasive vector. A brief contact (48h) between Aedes aegypti mosquito larvae and atrazine led to a modification of larval sensitivity to an insecticide: using atrazine as an inducer led to a decrease in the mortality of larvae treated with Bacillus thuringiensis var. israelensis (Bti).     

Human bioaccessibility of Cr, Cu, Ni, Pb and Zn in urban soils from the city of Torino, Italy

Several physiologically based extraction procedures have been proposed to estimate the fraction of the potentially toxic element content that would be bioaccessible in the human gastro-intestinal tract following accidental ingestion of soil. Many of these procedures are complex, they have been applied to a very limited range of soils, and most work has focussed on arsenic and lead. In the present study, a simplified, two-stage extraction, simulating the human stomach and intestine, was developed and applied to urban soil samples from ten public-access areas in the City of Torino, Italy. The human oral bioaccessibility of chromium, copper, nickel, lead and zinc was estimated. Lead and zinc bioaccessibilities were found to be higher in the stomach, but chromium was more bioaccessible in the intestine. Analyte concentrations were higher in roadside soils than in soils from parks. A higher proportion of the soil metal content was found in bioaccessible forms at roadsides than in parks. Comparison of the current findings with results of earlier work involving sequential extraction of the same soils indicated that the sequential procedure gave a relative, but not an absolute, indication of bioaccessibility. Calculations based on the bioaccessible analyte concentrations suggest that ingestion of only 2–3 g of some of the roadside soil samples from Torino could deliver the tolerable daily oral intake of chromium, nickel and lead to a 20-kg child. The developed procedure is useful for preliminary screening of soils and prediction of whether their bioaccessible metal contents are likely to pose a risk to human health.     

Speciation of mercury in South African coals

Total mercury (Hg_TOT) concentrations were determined by inductively coupled plasma mass spectrometry (ICP MS) for South African Highveld coals. The distribution of Hg in coals was investigated using a four-stage sequential leaching protocol and isotope dilution/gas chromatography coupled to ICP MS (ID-GC-ICP MS). The results show that Hg_TOT ranged from 144 to 303?µg?kg^?1 with a mean of 199?±?26?µg?kg^?1, while Hg_TOT leached from coals using different solvents ranged between 103 and 310?µg?kg^?1 (mean: 218?±?60?µg?kg^?1). Hg leaching rates of 53–78% were achieved in crushed coals. Hg⁰, Hg²⁺, and CH₃Hg⁺ were identified in all coals. CH₃Hg⁺ in studied coals ranged between 0.1 and 0.4 (mean: 0.2) µg?kg^?1. GC ICP MS chromatograms also showed unknown Hg peaks which were identified as other organomercury species such as ethylmercury. Modes of occurrence of Hg in coals were variable with the organic-bound (37–40%) and the sulfide-bound (37–39%) being the dominant mercury forms. Increasing the HCl concentration in the used protocol increased the amount of Hg leached (16%) during this step.     

Sex ratio bias and reproductive strategies: what sex to produce when?

Several theories predict the evolution of bias in progeny sex ratio based on variations in maternal or offspring reproductive value. For mammals, however, tests of sex-bias theories have produced inconsistent results, and no clear patterns have emerged. Each theory is based on assumptions that are difficult to satisfy, and empirical tests require large data sets. Using a long-term study on bighorn sheep (Ovis canadensis), we identified several parameters that influence progeny sex ratio according to maternal state. For older females, progeny sex ratio was affected by an interaction between reproductive strategy and environmental conditions. When conditions were good, old females reproduced every year but minimized fitness costs by producing daughters. When conditions were poor, old females produced more sons but did not reproduce every year. Sons of older females were of similar mass to those born to younger females under poor conditions but were smaller and likely disadvantaged under good environmental conditions. For young and prime-aged females, progeny sex ratio was independent of environmental conditions. Environmental conditions and age should be considered when studying sex ratio bias, which appears to be a function of maternal state rather than of maternal condition. We suggest that a conservative reproductive strategy drives progeny sex ratio in older females according to the "cost of reproduction hypothesis." By manipulating offspring sex ratio, older females reduced the cost of reproduction and increased their expected fitness returns.